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Iman Rusmana
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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 398 Documents
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Selection of Isolates of Entomopathogenic Fungi and the Bioefficacy of Their Liquid Production against Leptocorisa oratorius Nymphs SITI HERLINDA; SRI INDAH MULYATI; . SUWANDI
Microbiology Indonesia Vol. 2 No. 3 (2008): December 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.708 KB) | DOI: 10.5454/mi.2.3.9

Abstract

Entomopathogenic fungi are fungi pathogenic to insects and are widely used as biocontrol agents for insect pests. The aim of this research was to study the virulence of Beauveria bassiana and Metarhizium sp. isolates and to evaluate the efficacy of liquid production of those fungi against Leptocorisa oratorius (rice bug). Twelve isolates of B. bassiana and five isolates of Metarhizium sp. were used in this research. Selection result of B. bassiana isolates on third-instar rice bug nymphs showed that the isolate KBC caused the highest mortality rate (93%), while the lowest (46%) was caused by the isolate BBY 725. The shortest time needed to produce 50% mortality (Lethal time, LT50) was 3.52 days (isolate KBC). The longest time (10.36 days) was produced by isolate SLSS. The mortality of rice bug nymphs caused by Metarhizium isolates was only 50-62%. The shortest LT50 of Metarhizium (5.75 days) was produced by isolate Mtm, while the longest (7.46 days) was produced by isolate Mpx. Bioefficacy tests on six kinds of liquid formations of entomopathogenic fungi indicated that all were effective, mostly with LT50 d” two days. The mortality rates of rice bug nymphs caused by bioefficacy of fungus liquid production was generally above 85% up to 100%. The liquid media for entomopathogenic fungi performed better compared with solid media (SDA), as indicated by the greater mortality rate and shorter LT50.
The Influence of Oil Concentration, Nitrogen and Phosphorus Composition on Crude Oil Biodegradation by Epyzim and Mixed Cultures of Pseudomonas aeruginosa and Arthrobacter simplex ERLIZA NOOR; LINAWATI HARDJITO
Microbiology Indonesia Vol. 2 No. 3 (2008): December 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.721 KB) | DOI: 10.5454/mi.2.3.10

Abstract

Biological methods have gained attention as an alternative treatment for oil degradation in pollution remediation. External and internal factors have a great influence on crude oil biodegradation. This experiment studied the effect of oil concentrations and ratios of Ammonium and Phosphate on oil degradation in mixed cultures of local strains of Pseudomonas aeruginosa and Arthrobacter simplex. The oil degradation ability of this mixed culture was compared to the dormant culture of Epyzim. The increase of oil concentration, from 1, 3, 5 and 10% (w/v), significantly lowered the ability of both cultures to degrade the oil i.e from 83 % for 1% oil concentration to 64% for 10% oil concentration using local strains. The local strains showed better capability compare to the dormant culture. Medium composition was designed by three levels of ammonium concentration (7.6, 37.9 and 75.8 mg l-1) and two levels of phosphate concentration (2.0 and 9.9 mg l-1). The ratio of ammonium to phosphate of 3.8:1.0 in the growth media has resulted the maximum level of oil degradation, i.e 83% and 88%, for dormant and local cultures respectively. The results suggest a potential usage of local microorganisms in degrading crude oil-polluted water.
Influenza A Virus: Phylogeny of Neuraminidase Primers and Amplification of Polymerase Basic Protein 2 and Neuraminidase Genes MARIA OMEGA; RICHARD LAI; HANS HEINE; ROSS BARNARD
Microbiology Indonesia Vol. 3 No. 1 (2009): April 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.392 KB) | DOI: 10.5454/mi.3.1.1

Abstract

Influenza A virus is a highly contagious agent that causes bird flu. To date, 16 hemagglutinin (HA) and 9 neuraminidase (NA) subtypes are identified antigenically and can form any combinations or mutations with each other to confer non or low pathogenic to high pathogenic strains. Mutations in viral segments that are derived from avian isolates represent a novel subtypes to which human population is infected by influenza pandemics. In this work, polymerase basic protein 2 (PB2) gene segment of 8 different avian influenza subtypes were cloned to obtain more DNA samples for future work such as PB2 sequencing and to test HA primer annealing with PB2 gene. PCR amplification of NA gene segment of 3 different avian influenza subtypes was the second aim of this work to test primer universal for NA genes. Determination of the aligned sequences between 9 NA subtypes and NA primer PCR products was the second aim of this work, based on BLAST result homology 100% and phylogenetic trees of clustal
Genetic Diversity of Plant Growth Promoting Rhizobacteria of Bacillus sp. Based on 16S rRNA Sequence and Amplified rDNA Restriction Analysis SYAMSUL BAHRI SYAMSUL BAHRI; ARIS TRI WAHYUDI; NISA RACHMANIA MUBARIK
Microbiology Indonesia Vol. 3 No. 1 (2009): April 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.782 KB) | DOI: 10.5454/mi.3.1.2

Abstract

Plant-growth promoting rhizobacteria (PGPR) are rhizosphere associated soil-borne bacteria that can enhance plant growth and inhibit the development of root pathogens. Many soil bacteria have been used as PGPR, and one of them is Bacillus sp. The implementation of PGPR is constrained by genotype fluctuation that makes it inactive on the rhizosphere. Our previous study had characterized and revealed that 11 Bacillus sp. isolated from the soybean plant rhizosphere were PGPR. To asses and compare the genetic diversity of these isolates, Amplified Ribosomal DNA Restriction Analysis (ARDRA) and DNA sequence analysis of 16S rRNA were conducted. The construction of Neighbor-joining trees and bootstrap analysis of 100 resamples of ARDRA and 16S rRNA gene sequences were performed using Treecon software for windows ver. 1.3b. ARDRA analysis was done by using four restriction enzymes (RsaI, HaeIII, CfrI and HinfI), resulting in four phylotypes, respectively phylotype I (Bacillus sp. Cr24, Cr33, Cr64 and Cr68), phylotype II (Bacillus sp. Cr 31 and Cr66), phylotype III (Bacillus sp. Cr44 and Cr71) and phylotype IV (Bacillus sp. Cr67, Cr28 and Cr69). Results of BLASTN from 16S rRNA gene sequences showed that these isolates are genetically diversed. The evolution relationship of Bacillus sp. could be shown by the 16S rRNA gene sequences analysis, while ARDRA based on the digestion sites showed their variability.
Effect of pH, Temperature and Medium Composition on Xylanase Production by Bacillus sp. AQ-1 and Partial Characterization of the Crude Enzyme BUDIASIH WAHYUNTARI; NISA RACHMANIA MUBARIK; SISWA SETYAHADI
Microbiology Indonesia Vol. 3 No. 1 (2009): April 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.489 KB) | DOI: 10.5454/mi.3.1.3

Abstract

Bacillus sp. AQ-1 was isolated from household aquarium sediment. The isolate produced extracellular xylanolytic enzymes on xylan containing agar medium. Based on morphological, and physiological analysis, the isolate was identified as Bacillus sp. AQ1. The effect of temperature and pH on isolate growth and xylanase production were observed. The best condition observed for the enzyme production in Luria Broth supplemented with 0.5% oat spelt xylan medium was at 40 °C pH 7. The maximum enzyme production was 0.23 U mL-1 after 20 h of fermentation. Two different medium compositions (A and B) were examined for xylanase production. The maximum growth of the isolate and the xylanase production was better in A medium. Replacing oat spelt xylan in medium A with fruitless oil palm bunch in the medium caused the growth slightly slower than that of in the original formula. However, the xylanase production was 3 times higher in fruitless oil palm bunch medium. Optimum activity of the crude enzyme was observed at 60 °C and pH 7. Each ml of the crude enzyme contained 55.21 U xylanase, 8.12 U amylase and 0.50 U carboxymethylcellulase
Production and Purification of Xylanase From Indonesian Isolate Bacillus sp. AQ-1 Grown on Bunch Palm Oil PUJI RAHAYU; SISWA SETYAHADI; . HARMITA
Microbiology Indonesia Vol. 3 No. 1 (2009): April 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.89 KB) | DOI: 10.5454/mi.3.1.4

Abstract

Xylanase (endoxylanase, EC 3.2.1.8) is a commercial enzyme that has been applied in the industrial production of fuel, food, textiles and paper. Xylanase was isolated from the culture supernatant of Bacillus sp. AQ-1 grown on Nakamura medium containing 0.5% powder bunch palm oil. The optimum pH and temperature of xylanase activity were pH 7.0 and 60 °C, respectively. The enzyme was purified by anion exchange chromatography using DEAE-Sepharose-Fast-Flow column and gel filtration chromatography using Sephacryl S-300 column. The results showed that purification of xylanase produced two forms of xylanase, which were identified as xylanase A and xylanase B. Xylanase A can be separated from xylanase B by ultrafiltration using a 30 kDa polyethersulfone membrane. The molecular weight of xylanase A and B were 15.7 and 57.7 kDa, respectively.
The b’x Region of Yeast Protein Disulfide Isomerase is Not Essential for Saccharomyces cerevisiae Viability at 30 °C . PURKAN; LALU RUDYAT TELLY SAVALAS; MULIAWATI SINDUMARTA; DESSY NATALIA
Microbiology Indonesia Vol. 3 No. 1 (2009): April 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.074 KB) | DOI: 10.5454/mi.3.1.5

Abstract

Protein disulfide isomerase (PDI) catalyzes thiol oxidation, reduction and isomerization of disulphide bond of cell surface and secreted proteins. Yeast PDI1 consists of two catalytic domains (a and a’) which are separated by two non-catalytic domains (b and b’), and a x region linked the b’ and a domains. The b’ domain is important for the non-covalent binding of partially folded protein. To understand the contribution of b’ domain and x-linker of yeast PDI1 we have deleted the b’x and investigated its functional role in vitro and in vivo. Yeast PDI1 without b’x region retained only 50% activity and became more sensitive toward Proteinase K. Interestingly, yeasts containing full length PDI1 and pdi1Db’x showed approximately the same growth rate. However, the yeast pdi1Db’x mutant growth impaired severely at 37 °C compared to that of the full length PDI1. Our results suggested that the a-b-a’-c domains of PDI seems to be sufficient to support the growth of yeast cells in normal condition, but the b’x region might be essential in assisting refolding of highly accumulated unfolded protein at high temperature (37 °C).
Detection of Listeria monocytogenes in Pasteurized Milk Sold in Bogor and Its Relationship with Human Health AGATHA WINNY SANJAYA; MIRNAWATI SUDARWANTO; KIBUUKA ROBERT
Microbiology Indonesia Vol. 3 No. 1 (2009): April 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (54.831 KB) | DOI: 10.5454/mi.3.1.6

Abstract

Many countries have established a zero tolerance policy, under which ready-to-eat foods are contaminated with Listeria monocytogenes at a detectable level. The research was done in two parts. The first part was to qualitatively identify the presence of L. monocytogenes in pasteurized milk (n=32 samples) sold in different supermarkets in Bogor. The method was adopted from the Bacteriological Analytical Manual/Food and Drug Administration. All samples tested resulted negative to L. monocytogenes. The second part of the research was to evaluate the growth of L. monocytogenes in sterilized milk stored in an incubator set at 4oC and monitored for 7 days. The original L. monocytogenes culture at a concentration of 1x109 cfu mL-1 was diluted with buffered phosphate water 0.1% to reach a cell concentration of approximately 1.0 x 102 cfu mL-1. Growth was observed on the first, second, third, fourth and fifth day. On the sixth and seventh day, the numbers of colony forming units observed were almost similar (2.5-2.8 x 105 cfu mL-1). A population of 10 cells is sufficient to cause serious listeriosis infection in human.
Membrane Topology of Subunit 8 Variant of Yeast Saccharomyces cerevisiae Mitochondrial ATP Synthase I MADE ARTIKA
Microbiology Indonesia Vol. 3 No. 1 (2009): April 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.003 KB) | DOI: 10.5454/mi.3.1.7

Abstract

The yeast mitochondrial F1F0-ATP synthase is a multisubunit complex that contains at least 17 different subunits. Subunit 8 of yeast mitochondrial ATP synthase is a hydrophobic protein of 48 amino acids encoded by the mitochondrial ATP8 gene. There is no homologue of subunit 8 found in bacteria. Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain. Subunit 8 has been shown to adopt a transmembrane topology with the central hydrophobic domain spans the inner mitochondrial membrane once. In order to elucidate the need of subunit 8 to maintain transmembrane topology for its functioning, a severely functionally defective subunit 8 variant that has been introduced with double-charged residues within the central hydrophobic domain was analysed. A gene encoding this variant was expressed in a yeast strain lacking endogenous subunit 8. The subunit 8 variant was then targeted into mitochondria. Following its assembly into mitochondrial ATP synthase complex, its membrane topology was determined. The results obtained showed that subunit 8 was obligatory to maintain a transmembrane topology for providing proper functioning. The transmembrane topology may be critical for subunit 8’s proposed tructural roles as part of the stator stalk of the mitochondrial ATP synthase complex.
Neighboring Plants Alleviate Aluminum Toxicity on The External Hyphae of Gigaspora margarita AGUS ROHYADI
Microbiology Indonesia Vol. 3 No. 1 (2009): April 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (81.739 KB) | DOI: 10.5454/mi.3.1.8

Abstract

Excessive soluble aluminum (Al3+) in acidic soils is toxic to the external hyphae of arbuscular mycorrhizal fungi but it can be alleviated by other soil factors. A glasshouse experiment was conducted to study the effect of increased Al3+ concentration on the growth of the external hyphae of Gigaspora margarita in the presence of other plants near the host plants. The experiment used compartmentalized pots to facilitate the growth of mycorrhizal-inoculated host plants, external hyphae of the fungus and not mycorhizal-inoculated neighboring plants in different compartments; and measuring the effects of Al3+ and of the neighboring plants on the growth of the fungal hyphae independently. Increased concentration of Al3+ in soil affected the growth of external hyphae of G. margarita negatively. However, the hyphal length density of the fungus was much higher in the pots with neighboring plants than that in the other ones, despite the Al toxicity. This indicates that the hyphae could be taken away from the toxic effect of Al3+ by the stimulating growth from roots of the neighboring plants.

Page 27 of 40 | Total Record : 398

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