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JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya
Published by Universitas Negeri Malang
ISSN : 25496565     EISSN : 25496573     DOI : 10.17966
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
JC-T (Journal Cis-Trans) : jurnal kimia dan terapannya is published by Universitas Negeri Malang, Faculty of Mathematics and Science, Chemistry Department.
Arjuna Subject : Kimia(semua kategori) - Kimia (Lain-Lain)
Articles 5 Documents
 1 
Search results for , issue "vol. 2 no. 1 (2018)" : 5 Documents clear
Aktivitas Antioksidan Sari Rimpang Temu Giring (Curcuma heyneana) Terfermentasi Lactobacillus bulgaricus Ernanin Dyah Wijayanti; Linda Jausicha Yustin
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 2 No. 1 (2018)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v2i12018p001

Abstract

Temu giring (Curcuma heyneana) telah lama digunakan oleh masyarakat Indonesia terutama untuk menjaga kesehatan kulit. Senyawa kimia yang terdapat dalam temu giring adalah flavonoid, kurkumin, dan fenolik. Fermentasi pada sari temu giring dilakukan agar senyawa kompleks dipecah senyawa yang lebih sederhana. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan hasil sari temu giring (Curcuma heyneana) terfermentasi Lactobacillus bulgaricus. Tahap penelitian ini meliputi penyaringan rimpang temu giring, fermentasi temu giring, skrining fitokimia, pengujian aktivitas antioksidan analisis data dan membuat kesimpulan. Pembuatan sari temu giring dilakukan dengan menyari temu giring dengan air sampai didapatkan sari. Dilanjutkan dengan fermentasi menggunakan bakteri Lactobacillus bulgaricus selama 24 jam dengan suhu 37°C. Pengujian organoleptis meliputi warna, bau, rasa dan pH dilakukan. Pengujian skrining fitokimia sari temu giring segar dan terfermentasi positif mengandung fenolik, flavonoid, kurkumin. Pengujian aktivitas antioksidan menggunakan metode spektrometer UV-Vis dengan panjang gelombang 520 nm. Kesimpulannya bahwa sari temu giring terfermentasi memiliki aktivitas antioksidan dengan IC50 sebesar 3,49 ppm yang tergolong sangat kuat.
Perbandingan Kadar Fenolik Total Antara Seduhan Daun Gaharu Dan Kombucha Daun Gaharu (Aquailaria malaccensis) Nurmiati Nurmiati; Ernanin Dyah Wijayanti
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 2 No. 1 (2018)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v2i12018p006

Abstract

Agarwood leaf contains flavonoid, alkaloid, terpenoid, steroid, triterpenoid, so it is potential as anantioxidant. Fermentation can increase the antioxidant activity due to the increasing of phenoliccompound. This study aims to determine the comparison of total phenolic content of agarwood leaf brewand agarwood (Aquailaria malaccensis) leaf kombucha. The agarwood leaf simplicia was prepared bybrewing it at 80°C for 30 minutes, then fermented with kombucha for 10 days with 10% of sugaraddition. The organoleptic testing includes dye, flavor, aroma and pH. The agarwood leaf brew andkombucha were identified their phenolic, flavonoid, tannin, and anthraquinone. Finding shows that theypositively contain phenolic, flavonoid, tannin. The total phenolic content was tested by the FollinCiocalteau method. The determination result shows that total phenolic content of agarwood leaf brewis equal to 28.524 ± 0.359 mgGAE/gram and the total phenolic of agarwood leaf kombucha is equal to62.857 ± 2.104 mgGAE/gram. It can be concluded that there is a difference of total phenolic contentbetween the agarwood leaf brew and agarwood leaf kombucha, which has increased after thefermentation.
Efektivitas Antibakteri Ekstrak Etanol Daun Melinjo (Gnetum Gnemon L.) Terhadap Bakteri Escherichia Coli Nur Candra Eka Setiawan; Anny Indah Widianti
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 2 No. 1 (2018)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v2i12018p012

Abstract

Melinjo (Gnetum gnemon L.) is one of the plants which leaves can be used as antibacterial of the issuediarrhea problem because melinjo leaves contains alkaloids, flavonoids, saponins and tannins. One ofthe caused diarrhea is bacterium Escherichia coli. The purpose of this study was to determine theactivity and effectiveness of the antibacterial ethanol extract of melinjo leaves against Escherichia colibacteria. This study included the type of experimental research was conducted in the Mikrobiologylaboratory and Farmakognosi laboratory at Academy of Pharmacy of Putra Indonesia Malang. Thisresearch used diffusion method of wells with concentrations of 10% w/v, 20% w/v, 30% w/v, 40% w/v,50% w/v, 60% w/v, 70% w/v, 80% w/v, 90% w/v, and 100% w/v. Result of the study showed that ethanolextract of melinjo leaves can inhibit the growth of Escherichia coli bacteria. Effective concentration ofantibacterial against Escherichia coli was 90% w/v. The conclusion of this study of melinjo leaves ofethanol extract has activity and effectiveness antibacterial to bacterium Escherichia coli
Optimasi Metode Analisis Injeksi Alir pada Analisis Surfaktan Anionik Hanumi Oktiyani Rusdi; M. Bachri Amran
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 2 No. 1 (2018)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v2i12018p018

Abstract

Surfactant is an active ingredient in the detergent, soap, shampoo and others. Using large numbers ofsurfactant has an impact on environmental contamination, such as water pollution. It is necessary to determinethe concentration of surfactant in contaminated water. Determination of surfactant is generally done withvisible light spectrometry method through the extraction process. However, the extraction process is timeconsuming and requires a number of solvents which cause many of these methods are not effective and efficientin analysis. In this research, the analytical method of anionic surfactant has been developed based on flowinjection analysis using preconcentration techniques with C18 mini-column and detection by visible lightspectrometry. Anionic surfactant (Sodium Dodecyl Sulfate/ SDS) made ion pair formation with cationic dyes(Brilliant Green/ BG) in C18 mini-column. Ion pair can be eluted with organic solvents such as methanolchloroform (1:9). The visible spectrum of BG-SDS ion pair has a maximum absorption at 629 nm. The resultsalso show that BG-SDS ion-pair formed optimum at pH 6. Preliminary analysis showed a linear relationshipbetween SDS concentration variations with ion-pair absorbance value. The increasing concentration of SDScauses the absorbance value increase.
Inhibisi Xantin Oksidase oleh Ekstrak Daun Salam (Syzygium Polyanthum) Nur Candra Eka Setiawan; Ana Nurjanah
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 2 No. 1 (2018)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um026v2i12018p025

Abstract

Xhantine oxidase is an enzyme that plays a role in the formation of uric acid. Xanthine oxidase catalyzeshypoxanthine into xantin and then becomes uric acid. Bay leaf plants can lower blood uric acid levelbecause it contains secondary metabolites such as flavonoid, tannin, and essential oil. This reseachaimed are to find out xhantine oxidase inhibition by bay leaf extract. This reseach use experimentalresearch method which done in pharmacognosi of pharmacy of Putra Indonesia Malang. The reseachinclude extraction, isolation of xanthine oxidase enzyme, enzyme activity test and enzyme inhibition test.Testing of enzyme activity of xanthine oxidase inhibition using spectrophotometric at λ290 nm. Theresult showes that in variant concentrations of 5 ppm, 10 ppm, 20 ppm, 50 ppm dan 100 ppm wereproduced inhibitions is %, 0%, 100%, 100%, 0%. Allopurinol is used as a positive control with the sameconcentration, the % inhibition is 100%, 0%, 0%, 0%, dan 100%. Based on the result, the bay leafextract with a concentration of 5 ppm has 100% inhibition equivalent to allopurinol 5 ppm.Concentration 100 ppm of bay leaf extract not show inhibitory activity.

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