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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 6 Documents
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Search results for , issue "Vol. 14 No. 3 (2024)" : 6 Documents clear
Assessment of Vitamin D Deficiency Prevalence among Type 2 Diabetes Mellitus Patients in a South Indian Tertiary Care Hospital Kalimuthu, Senthilkumar; Philip, Sachu; Elango Nandhini, Natarajan; Baba, Lalamiya Abdul Rahiman; Ravichandran, Shanmugasundaram
The Journal of Experimental Life Science Vol. 14 No. 3 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.03.01

Abstract

Vitamin D deficiency has become a global health issue and is associated with the multifactorial clinical manifestations of diabetes. The objective of this study is to analyze vitamin D deficiency in T2DM patients in association with biochemical parameters and thyroid-stimulating hormone (TSH). This study was a cross-sectional study in a tertiary care hospital conducted after the Institutional Ethical Committee (IEC) approval. The available descriptive data of patients, such as age, gender, biochemical parameters, TSH, and glycated hemoglobin (HbA1c), were collected. Vitamin D was measured using the enzyme-linked immunosorbent assay (ELISA) method. The descriptive parameters were statistically analyzed using chi-square analysis. The results comprise 322 T2DM patients, with 187 males and 135 females. Vitamin D status levels were observed to have severe deficiency < 10 ng.mL-1 (23.6%), deficiency >10-20 ng.mL-1 (42.2%), insufficient >20-29 ng.mL-1 (22.7%), and sufficient > 30 ng.mL-1 (11.5%). The male and female patients with vitamin D status were significantly (p<0.001) different between groups. No significant (p = 0.122) association was observed between HbA1c and vitamin D. The high status of vitamin D deficiency with high glycemic levels is associated with poor diabetic control. Therefore, patients require awareness about their vitamin D status; with a proper diet, adequate exposure to sunlight, and exercise can help them improve their health. Keywords: Cholesterol, HbA1c, TSH, Type 2 diabetes mellitus, Vitamin D.
The Comparison Effect of DOCA and L-NAME on T Cells Activation in the Preeclampsia Mice Model Khodijah, Riska Amalia; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rahayu, Sri; Rifa'i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 14 No. 3 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.03.03

Abstract

Preeclampsia is a systemic disorder of pregnancy characterized by the maternal immune system's failure to properly adapt to fetal antigens, resulting in chronic inflammatory responses. This research aimed to examine the effects of DOCA (deoxycorticosterone acetate) and L-NAME (Nω-nitro-L-arginine methyl ester) in inducing preeclampsia in pregnant BALB/c mice, focusing on lymphocyte activation. Twenty-four BALB/c mice were randomly divided into four groups: NP (non-pregnant mice), P (healthy pregnant mice), D (pregnant mice received DOCA), and L (pregnant mice received L-NAME). DOCA was dissolved in corn oil and administered subcutaneously at 12.5 mg.kg-1 BW before pregnancy and 6.5 mg.kg-1 BW weekly during pregnancy. L-NAME was administered daily starting from day 5 of pregnancy. Mice were dissected on day 16 of pregnancy, and the spleen was isolated to analyze the relative number of CD4+CD62L+ and CD8+CD62L+ T cells. The results showed that DOCA and LNAME exhibited fewer CD4+CD62L+ and CD8+CD62L+ T cells than healthy pregnant mice. The administration of DOCA at 12.5 mg.kg-1 BW (before mating) and 6.5 mg.kg-1 (day 7 of pregnancy) was more effective in triggering the activation of CD4+CD62L+ and CD8+CD62L+ T cells than the others indicated by the lowest number of CD4+CD62L+ and CD8+CD62L+ T cells. However, the conclusion of which one is more effective in inducing preeclampsia has not been reached. This is due to the many parameters that must be observed related to preeclampsia. Further studies are needed, especially examining proinflammatory cytokine production in T cells after induction with both inducers. Keywords: DOCA, L-NAME, Lymphocyte, Mice, Preeclampsia.
Evaluation of TNF-α and IFN-γ Levels in Macrophage of Preeclampsia Mice Model Induced by DOCA and L-NAME Susanti, Winda Karina; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rahayu, Sri; Rifa'i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 14 No. 3 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.03.05

Abstract

Preeclampsia is a pregnancy disorder characterized by high blood pressure, proteinuria, and chronic inflammation that potentially leads to multiorgan failure. This study investigated differences in the relative number of pro-inflammatory cytokines (IFN-γ & TNF-α) in preeclamptic mice induced by DOCA and L-NAME. Eighteen pregnant BALB/c mice (n=6): pregnant mice without any treatment (P), pregnant mice treated with Deoxycorticosterone acetate/DOCA (D), and pregnant mice treated with N-ω-L-arginine Methyl Ester/L-NAME (L) and healthy nonpregnant mice as control (NP). DOCA at 12.5 mg.kg-1 BW was injected subcutaneously before mating and 6.5 mg. kg-1 BW after mating every week for 16 days. L-NAME was administered orally at 75 mg.kg-1 BW daily after five days of gestation. Mice were dissected in the second trimester (day 16), and then the spleen was isolated to analyze the relative number of CD11b+IFN-γ+ and CD11b+TNF-α+ using flow cytometry analysis. The results showed that DOCA and L-NAME induction did not significantly induce the production of TNF-α levels in the macrophage of pregnant mice. However, both DOCA and L-NAME significantly induce the production of IFN-γ levels in the macrophage of pregnant mice. Further evaluation of other markers related to preeclampsia should be done to better understand the roles of DOCA and L-NAME in inducing preeclampsia. Keywords: DOCA, L-NAME, mice, preeclampsia, pregnant.
Evaluating SARS-CoV-2 Spike Protein Transfection in HEK-293T Cells for VLP Applications Rohmah, Ilmiana Nurur; Hafil Kusuma, Kavana; Ika Christina, Yuyun; Rizqi Dwijayanti, Dinia; Mustikaningtyas, Dewi; Widodo, Nashi; Sasmito Djati, Muhammad
The Journal of Experimental Life Science Vol. 14 No. 3 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.03.02

Abstract

The spike protein in SARS-CoV-2 works mainly to initiate infections, especially for binding to host cells and fusing with cell membranes. Characterizing SARS-CoV-2 spikes protein transfection cells could optimize the production of VLP in HEK-293T cells. Therefore, the present study aimed to investigate the characteristics of SARS-CoV-2 spike protein expression in transfected HEK-293T cells. Characterization of spike protein in transfected-HEK 293T cells was used to determine VLP formation in SARS-CoV-2. The study was conducted by isolating DNA and protein from transfected HEK 293T cells and analyzing spike expression using Polymerase Chain Reaction (PCR) and Flow Cytometry. The success of the SARS-CoV-2 spike protein transfection in PCR analysis has yet to be confirmed because DNA band smears do not specifically indicate the spike protein's size. However, the SARS-CoV-2 spike protein was successfully confirmed using flow cytometry with the percentage of spike-EGFP by 30%. Therefore, the present study confirmed the presence of spike protein in transfected HEK 293T cells by flow cytometry analysis. Keywords: Coronavirus, Characterization, DNA, Spike, Virus-Like Particle (VLP)
Analysis of Urban Groundwater Quality in Dryland Areas of Kebbi State, Nigeria Abubakar Usman, Abdulqadir; Wali, Saadu Umar; Shera, Ibrahim Muhammad
The Journal of Experimental Life Science Vol. 14 No. 3 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.03.04

Abstract

This study aims to assess the physicochemical composition of groundwater in the dryland area of Kebbi State, Nigeria. Groundwater samples were collected from four urban areas – Argungu, Birnin Kebbi, Gwandu, and Kalgo. Forty water samples from deep wells, i.e. boreholes/hand pumps, were obtained and analyzed. Twelve physicochemical parameters were analyzed, and these include Electrical conductivity (EC), pH, Total Dissolved Solids (TDS), Chloride (Cl-), Potassium (K+), Calcium (Ca2+), Iron (Fe3+), Magnesium (Mg2+), Zinc (Zn2+), Cupper (Cu2+) Bicarbonates (HCO3-) and Sodium (Na+). The Weighted Arithmetic Water Quality Index (WAWQI) assessment of groundwater quality for the four urban areas revealed that 45% of the water sources were good quality, 35% were poor quality, and 5% were very poor quality. A 15% were unsuitable for drinking due to the high concentration of EC, TDS, Fe3+, Cu2+, and Ca2+ ions. The physicochemical examination of groundwater from four urban areas showed results that satisfied the WHO drinking water standard. Results from a non-parametric statistical test (Kruskal-Wallis) showed a significant difference in EC and TDS are significant at < 0.001 in the groundwater of Gwandu, while no significant differences exist in the physicochemical parameters of Argungu, Birnin Kebbi, and Kalgo. Pearson’s correlation analysis indicated that rock weathering controls groundwater quality primarily. However, correlations between TDS and other physicochemical parameters are indications of anthropogenic contributions to groundwater pollution in the study area.  The overall assessment of the Water Quality Index in the study area indicates that groundwater needs treatment, proper maintenance, and compliance with the world standard. Keywords: Groundwater, Physicochemical parameters, Water quality, Weighted Arithmetic Water Quality Index (WAWQI).
Abundance and Characteristics of Microplastics in Coastal Sediment and Seawater Collected from Surabaya and Tulungagung Indriyasari, Khudrotul Nisa; Rahmatin, Nailul Muthiati; Marchellina, Ary; Mukholladun, Wildanun; Soegianto, Agoes
The Journal of Experimental Life Science Vol. 14 No. 3 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.03.06

Abstract

This research attempted to determine the abundance and characteristics, including morphology, color, and size of microplastics found in coastal sediment and seawater of Wonorejo, Surabaya, which is located on the north coast of East Java, and Popoh, Tulungagung on the south coast of East Java. Water and sediment samples were collected during the dry season with two replications. The standard method of water sample laboratory preparation and microplastic identification was carried out using NOAA Technical Memorandum NOS-OR&R-48, while the sediment sample was prepared with several modifications. The average microplastic abundance in Surabaya and Tulungagung seawater samples is equal to 2100 particles m-3 and 170 particles m-3, respectively. Meanwhile, as many as 740 particles kg-1 and 350 particles kg-1 were found consecutively in sediment samples of Surabaya and Tulungagung. Microplastics in seawater samples were dominated by 44.7% red and 80.9% fiber, while 43.1% black and 51.4% fiber microplastics were primarily found in sediment samples. The microplastic size ranges from 30—1450 μm in seawater samples and 50—3750 μm in sediment samples. Keywords: abundance, characteristics, microplastics, seawater, sediment.

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