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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 8 Documents

Search results for , issue "Vol. 5 No. 2 (2015)" : 8 Documents clear

The Effectiveness of hrGFP Gene Reporter Role in Carp Fish (Cyprinus carpio) Transgenesis Process Based on Convocal Microscopy Analysis Septi Anitasari; Agus Soeprijanto; Abd. Rahem Faqih
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Sperm cells can be used as a medium to insert foreign DNA into the egg. Electroporation humanized renilla reformis Green Fluorescent Protein (hrGFP) gene as a reporter gene is very important to know the effectiveness of gene transfer. Research is aimed to understand the effectiveness rate of hrGFP gene reporter to detect the successful gene transfer process based on the result of observation with convocal microscope over transgenic sperm level, embryo and larva of Carp Fish. Result of trans-genesis of hrGFP as sperm-mediated gene reporter must be accurately detected. Based on the result of observation with convocal microscope, it is shown that hrGFP enters the core level, indicates by PI pigmentation and thereby, hrGFP gene reporter can provide information about hrGFP fluorescence starting from sperm, embryo and larva of Carp Fish.Keywords: Carp Fish, effectiveness, gene reporter, hrGFP, sperm fluorescence.

Antibacterial Activity of Some Herbs Water Extract against Escherichia coli Bondan Prakoso; Eko Widodo; Edhy Sudjarwo
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

The research aims to select the highest diameter inhibition zone of several types of herb water extract against Escherichia coli and find the best herb water extract towards minimum inhibition concentration. It was divided into two parts, in the first stage was to evaluate antibacterial activity of herbs water extract and the second stage was to evaluate concentration of extract to effectively inhibit growth of E. coli. In the first experiment there were 6 treatments; P0 (aquadest 100%), P1 (antibiotic tetrachlor), P2 (Zingiber officinale extract), P3 (Z. officinale var. Rubrum extract), P4 (Kaempferia galanga extract) and P5 (Z. zerumbet L. extract) tested to find the best herbs on the basis of diameter inhibition zone. In the second experiment 6 treatments were used namely from 0%, 50%, 60%, 70%, 80%, 90% and 100% of Z. officinale var. Rubrum extract tested for Minimum Inhibition Concentration (MIC) against E. coli. The results showed that among the herbs, Z. officinale var. Rubrum extract had the strongest antibacterial activity based on diameter of inhibition zone against E. coli. While as low as 50% of Z. officinale var. Rubrum extract showed an MIC although the highest activity indicated when 100% of Z. officinale var. Rubrum extract used.Keywords: Z. officinale var. Rubrum, diameter of inhibition zone, MIC and E. coli.

Effect of Maintenance at Different Salinity against White Spot Syndrome Virus (WSSV) Infection Level in Post Larvae Litopenaeus vannamei Shrimp Attabik Mukhammad Amrillah; Sri Widyarti; Yuni Kilawati
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

White spot syndrime virus (WSSV) is a highly contagious disease in shrimp culture and causing 100% death within 3-10 days of clinical symptoms. WSSV can infect shrimp in post larvae stage (PL) to a size of 40 g. This study was aimed to determine the effect of different salinity against WSSV infection level in post larvae vannamei shrimp (Litopenaeus vannamei). In this study PL 40 size of vannamei shrimp infected with WSSV in virus concentration 20 μg.mL-1 in three different salinity is 5 ppt, 15 ppt, 25 ppt for 4 hours using the soaking method then maintened for 7 days post-infection and then infection level and water quality observed. Data analysis used simple linear regression and F test with a confidence level 95% (P <0.05). The results indicate changes in behavior between healthy and infected shrimp in three levels (mild, moderate, severe). The highest total mild infection in salinity treatment of 25 ppt by 30%, the highest total medium infection at salinity treatment 25 ppt by 37%, the highest total severe infection at salinity treatment 5 ppt by 57%. Shrimp that have mild level of infection are increasing in concominant higher salinity, while the severe infection level has decreases with increasing salinity. Keywords: Infection Level, Litopenaeus vannamei, Post Larvae, Salinity, WSSV

Potency of Green Cincau Leaves (Premna oblogifolia merr) Juice as Prebiotics and its Effect on Laying Hen Performances Anna Lidiyawati; Eko Widodo; Edhy Sudjarwo
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

This study investigated the potency of green cincau leaves (Premna oblongifolia merr) juice as prebiotics end its effect on laying performances (feed intake, egg weight, feed conversion and hen day production) based on pH and digestive viscosity. The potency of green cincau leaves juice as prebiotic was tested. The result showed that green cincau leaves juice was effectively support growth of Lactic Acid Bacteria (LAB) with 4.3 x 102 CFU.g-1 samples but did not support the growth of Escherichia coli. The effect of green cincau leaves juice on laying performances was examined. Two hundred fifty 56-wk-old Hisex Brown layers received different diets namely 0, 5, 7.5, 10 and 12.5 ml of green cincau leaves juice added in the basal feed. The method used in this study was experiment based on Completely Randomized Design and followed by Duncanâ€™s Multiple test if significant effect occurs. The result showed that increase level of P. oblongifolia merr in feed did not significantly affect (P>0.05) feed intake, egg weight, feed conversion and hen day production.Keywords: feed additives, laying hens performance, prebiotic, Premna Â oblongifolia merr juice.

Tapak liman (Elephantopus scaber L) Extract Induced CD4+ and CD8+ Differentiation from Hematopoietic Stem Cell/Progenitor Cell Proliferation of Mice (Mus musculus) M. Sasmito Djati; Hindun Habibu; Nabilah A Jatiatmaja; Muhaimin Rifai
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Tapak liman (Elephantopus scaber L) is one of the traditional medicinal plants, which are containing several active compounds that potentially affecting hematopoietic. The aims of this study are to elucidate the effect of leaf extract of Tapak Liman on hematopoietic in mice BALB/c, by observation on relative number of cells expressing CD4/CD8, CD4/CD62L, and TER119/B220 in the spleen; TER119/B220, TER119/VLA-4 and TER119/CD34 in bone marrow, after given the leaf extract for 2 weeks. This experiment was used 12 female mice, which is divided on 3group treated with leaf extract of Tapak Liman (P1= 0.5 g. g bw-1.day-1, P2= 1.0 g. g bw-1.day-1 and P3 = 2.0 g. g bw-1.day-1) and control. The relative numbers of cells expressing surface molecules were analyzed by flowcytometry and quantitative data were tested using one way ANOVA. The result showed the leaf extract of Tapak Liman has no significant effect on erythrocyte proliferation, on the other hand it have significant effect on both proliferation and differentiation of B lymphocytes (B220+) in the bone marrow (p=0.044) and increase the expression of CD4+, CD8+molecule in B cells (p=0.026) and erytroid (TER119+VLA-4+) in spleen and bone marrow. The administration of 1 g. g bw-1.day-1 of the extract (P2) stimulate proliferation of lymphocytes and erythrocytes lineage (TER119+VLA-4+), in spleen and bone marrow.Keywords: CD4+, CD8+, Elephantopus scaber, erythrocyte, hematopoiesis.

Bioactivity of Sauropus androgynus and Elephantopus scaber to CD4+IL2+ and CD4+IL4+ T Cells Modulation in Balb/c Pregnant Mice Model of Typhoid Yayu Tsamrotul Fuadah; Moch. Sasmito Djati; Sri Widyarti
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Pregnant woman have higher risk to get infection, because pregnancy decreasing the cell T activity. Sauropus androgynus and Elephantopus scaber has substance like saponin and flavonoid which has been well known as natural imunomodulator, particularly to increase amount of immunocompetent cell. This research is important to recognize effective supplement supply for immunomodulator of S. androgynus and E. scaber to increase mice's (Mus musculus Balb/c) immune system. This research conducted in seven treatments by 3 repetitions for each treatment by using pregnant mice which has been infected by bacteria Salmonella typhimurium (dose 107 CFU.mL-1). Bacteria are injected to mice intraperitoneal in day 5th after giving combination of extract E. scaber and S. androgynus. The dose of E. scaber and S. androgynus combination are 200; 150:37.5; 100:75; 50: 112.5; 150. Five group of treatment were infected by S. typhimurium. Two other groups were the control, namely negative control which was only given NaCMC 0.05% without infection and positive control which was given NaCMC 0.05% and infected by S. typhimurium. After being injected, treatment was redone till the day of surgery. The surgery was executed in day 12th and 18th of pregnancy. Data were analyzed using ANOVA (p < 0.05) and Duncan test. Result indicated that extract of S. androgynus and E. scaber could increase amount of immune system in pregnant mice. This was indicated from significant increasing in amount of cell T CD4+IL2+ and CD4+IL4+ in pregnant mice which has been infected by S. thypimurium. Formula of extract S. androgynus and E. scaber which could return immune condition was approached condition of healthy pregnant mice such as E. scaber 200 mg.kg-1 BW; E. scaber 100 mg.kg-1 BW and S. androgynus 75 mg.kg-1 BW; E. scaber 50 mg.kg-1 BW and S. androgynus 112.5 mg.kg-1 BW; and S. androgynus 150 mg.kg-1 BW, respectively. Keywords: CD4+IL2+,CD4+IL4+, E. scaber, Immunomodulator, S. androgynus, S. typhimurium

Bioactivity of Combination Elephantopus scaber and Sauropus androgynus on the Level of B220 cells of Lymph Node in Pregnant Typhoid BALB/c Mice Lulut Dwi Nurmamulyosari; Muhammad Sasmito Djati; Sri Widyarti
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Elephantopus scaber and Sauropus androgynus are herbal remedies that contain flavonoids as immunomodulatory agents. This study was aimed to observe the changes in the relative number of B cells in lymph node tissue of pregnant typhoid mice. Mice were divided into seven groups K-, K+, E. scaber 200 mg.kg-1 BW, E. scaber 150 mg.kg-1 BW + S. androgynus 37.5 mg.kg-1 BW, E. scaber 100 mg.kg-1 BW + S. androgynus 75 mg.kg-1 BW, E. scaber 50 mg.kg-1 BW + S. androgynus 112.5 mg.kg-1 BW, dan S. androgynus 150 mg.kg-1 BW. Mice were dissected on the 12th and 18th day after herbal treatment. Data was analyzed using one way ANOVA (p < 0.05) and Duncan. The result show that E. scaber combination of 200 mg.kg-1 BW and E. scaber 150 mg.kg-1 BW + S. androgynus 37.5 mg.kg-1 BW can increase the number of B220 cells (p < 0,05) on pregnant mice typoid model. Keywords: B220, Elephantopus scaber, pregnant, Sauropus androgynus, typhoid fever

Effect of Cilembu Sweet Potato Starch and Storage Times on Physicochemical and Microbiology of Synbiotic Yoghurt Ice Cream Lestari Kusumah Dewi; Lilik Eka Radiati; Imam Thohari
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

The purpose of this research was to determine the effect of adding cilembu sweet potato starch (1-6 %) and storage time (2, 4, 6 weeks) on physicochemical and microbiology of synbiotic yoghurt ice cream. The research methodology used was experiment laboratory. The first experiment was designed by Completely Randomized Design (CRD) using 7 treatments and 4 replications, while second step was designed by CRD using 4 treatments and 4 replications. Data were analized by Analysis of Variance (ANOVA) and continued by Duncan's Multiple Range Test (DMRT). Determination of the best treatment used Effectiveness Index, which has been modified. The result of first step showed the best treatment is the addition of cilembu sweet potato starch 3% with value 5.400 log10 cfu.mL-1 of total LAB, 4.322 of pH, 0.200 g.L-1 of EPS, 40.257 P of viscosity, 35.523 minutes.50g-1 of melting rate, 30.258% of overrun. For second step, the best treatment is 6 weeks stored with value 5.004 log10cfu.mL-1 of total LAB, 4.347 of pH, 0.327 g.L-1 of EPS, 114.928 P of viscosity, 48.828 minutes.50g-1 of melting rate. Keywords: cilembu sweet potato,exopolysaccharides, ice cream, periods

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Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

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+6282333752235

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jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA