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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 11 Documents

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Search results for , issue "Vol. 7 No. 1 (2017)" : 11 Documents clear

Influence of Different Pulse Length towards Motility and Viability of Ornamental Japanese Carp (Cyprinus carpio Var. Koi) Sperm through Electroporation Method Diana Aisyah; Agoes Soeprijanto; Yuni Kilawati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2016.007.01.06

Successful studies about implementation of electroporation method which sperm becomes gene transfer medium for fish transgenesis have reported. Motility and viability are two major factors in successful of electroporation. The objective of the study was to determine optimal pulse length of electroporation towards the motility and viability of Koi fish sperm. The study was conducted at the Central Laboratory of Biological Sciences and Reproduction of Brawijaya University. The data analysis was conducted using completely randomized design. The electroporation method was carried out using voltage of 30 volt with pulse number of 4 times. The pulse length of the electroporation (based on treatment) was 0.5, 1 and 1.5 ms. The result showed the highest percentage of motility was 3.67% in treatment A (pulse length 0.5 ms) with coefficient correlation R2 = 0.9643 and the best percentage of viability was 79.67% in treatment A (pulse length 0.5 ms) with coefficient correlation R2 = 1. Correlation between the pulse length treatment (electroporation) toward motility and viability of Koi fish sperm, in which longer duration of pulse length treatment (electroporation) would decrease the motility and viability percentage of the fish sperm.Keywords: Electroporation, Motility, Pulse length, Sperm, Viability.

The Role of Local Hydromacrophytes in Leachate Phytoremediation Performed Using Constructed Wetland System Sophia Laily; Bagyo Yanuwiadi; Catur Retnaningdyah
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

The purpose of this research is to analyze the effectiveness of using local hydromacrophytes for performing leachate phytoremediation in constructed wetland (CW) system. It was an ex situ experiment carried out in a glass house by means of free water surface (FWS) CW reactors containing sand and gravel substrates in 3:5 ratio. The reactor was waterlogged by 9 L leachate. The phytoremediation tests were performed in two-factor treatment design involving hydromacrophyte species and hydraulic retention time. Species of local hydromacrophyte used in this research included Alternanthera sessilis, Commelina nudiflora, Paspalum conjugatum, Typha angustifolia and the polyculture of the four species. The improvements in leachate qualities were identified through decreases of physico-chemical parameters. Among the mean values of the percentages indicating the decreases of physico-chemical leachate parameters, the greatest ones were resulted from the treatments using T. angustifolia and A. sessilis on the 30th day. It has proven in the experiment that these two species of hydromacrophyte perform the most effective process of improving leachate quality.Keywords: constructed wetland, leachate, local hydromacrophytes, phytoremediation.

The Impact of Dissolved Nitrate and Phosphate on Maximum Growth Rate and Carrying Capacity of Oscillatoria in Intensive Shrimp (Litopenaeus vannamei) Farming Pond Situbondo, East Java, Indonesia Dian Aliviyanti; Suharjono Suharjono; Catur Retnaningdyah
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

The aims of study are to analyze the effect of dissolved nitrate and phosphate content of the intensive shrimp farming pond Situbondo to maximum growth rate and carrying capacity of Oscillatoria population density in the laboratory. This is an experimental research method using completely randomized design with three replications. The treatment were variation of nitrate and phosphate concentration (N0; N6; N12; N24; N48; P0,2; P0,4; P0.8, P1.6 mg.L-1). Experiment was done using a pure Oscillatoria culture in condition 25 watt lamp; 12 hours a day. The initial amount of Oscillatoria cells used for the treatment is 8 - 15 x 104 cell.mL-1. During the incubation process, chemical parameters were also observed including nitrate, phosphate, DO, and pH at the beginning of the incubation period. Oscillatoria cell was count every day until stationary phase for 30 days. Furthermore, a different test between treatments was conducted to determine levels of nitrate and phosphate in triggering the blooming of Oscillatoria using Oneway ANOVA analysis with SPSS Program. The results showed that the intensive shrimp pond waters of Situbondo already contain dissolved phosphate between 0.4 - 0.5 mg.L-1. Oscillatoria growth is strongly influenced by dissolved phosphate content in waters, phosphate levels of 0.2 - 0.4 or equivalent to the actual level of 0.6 - 0.7 mg.L-1 can cause the highest abundance of Oscillatoria that could endanger the ecosystem. Keywords: dissolved phosphate, intensive shrimp farming, Nitrate, Oscillatoria.

Extract of Caesalpinia sappan L. as Antibacterial Feed Additive on Intestinal Microflora of Laying Quail Anang Widigdyo; Eko Widodo; Irfan Hadji Djunaidi
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Caesalpinia sappan L. is a phytobiotic plant that serves as an antibacterial. Active compound such as flavonoids in the C. sappan L. extract acts as an antibacterial. TheÂ protein food sources in Indonesia including poultry such as quail. Several studies demonstrated that quail are very susceptible to bacterial infections including Escherichia coli and Salmonella. Thus C. sappan has been used as food additive for quail in laying eggs. This research objective was to study the effects of C. sappan L. extract against intestinal miclofora of quail in laying eggs. This study used 168 quails with 4 treatments and 5 replications, each treatment used 7 quails. This study used 4 Wood Extract C. sappan L. treatment with different concentrations including 0%, 0.2%, 0.4%, 0.6%. The results showed that administration of Extract Wood C. sappan L. was not significantly different (P> 0.05) to the total number of bacteria E. coli with the average P0 (6.0903 Log CFU), P1 (6.0903 log CFU), P2 (6.0887 Log CFU), and P3 (6.0868 Log CFU). Meanwhile, it was significantly different (P <0.05) to the total number of Salmonella bacteria in the gut laying with the average number of bacteria P0 (5.4059 Log CFU), P1 (5.4048 Log CFU), P2 (5.4045 Log CFU), and P3 (5.4039 Log CFU). It is concluded that flavonoids compounds in C. sappan L. extract could decrease the number of Salmonella, but not E. coli, in the intestine of quail.Keywords: Caesalpinia sappan L., Eschericia coli, Flavonoid, Salmonella.

Dynamical Analysis of Fractional-Order Hastings-Powell Food Chain Model with Alternative Food Moh Nurul Huda; Trisilowati Trisilowati; Agus Suryanto
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

In this paper, a fractional-order Hastings-Powell food chain model is discussed. It is assumed that the top-predator population is supported by alternative food. Existence and local stability of equilibrium points of fractional-order system are investigated. Numerical simulations are conducted to illustrate analysis results. The analysis results show that alternative food can give a positive impact for top-predator population.Keywords: Alternative food, Fractional-order, Grunwald-Letnikov approximation, Hasting-Powell model, Stability.

The Effect of Electroporation Method towards the Motility and Viability of Java Barb Fish (Puntius javanicus) Sperm Dimas Adetia Rikianto; Agoes Soeprijanto; Yuni Kilawati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Electric shock treatment of Java Barb Fish (Puntius javanicus) sperm using electroporation method on sperm as transfer gen (Sperm Mediated Gen Transfer) has not been implemented in Indonesia. This study was conducted to know the effect of electric shock using gene pulser at different voltage level toward motility and viability of Java barb fish sperm. This research was conducted at Fish Breeding Laboratory, Faculty of Marine and Fisheries and LSIH of Brawijaya University-Malang in May 2017. The trial design used Complete Randomized Design with 3 different treatments and 1 control which each treatment repeated 3 times. The treatments used the Gene pulser with 3 different voltages: A (20 V), B (30 V), C (40 V). The result showed that the electric shock treatment with different voltages level affected motility and viability of Java barb fish sperm. Based on the data analysis used polynomial orthogonal, a linear-form of the relationship among the treatments in the form of equation was found (Puntius javanicus) with R2=0.9815 and equation y = -40.5x + 130.83 with R2= 0.8626. Based on the result of this research, electroporation for Java barb fish' sperm as gene transfer media should be done with voltage 20 V, pulse number 4 times and pulse length 1 ms. Keywords: electroporation, motility, Puntius javanicus, sperm, viability.

The Effect of Organic Stimulant and Inorganic Fertilizer on Two Rice Varieties (Oryza sativa L.) Erningtyas Widyaswari; Mudji Santosa; Moch. Dawam Maghfoer
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Increased the yield of rice could be done by organic stimulant application and inorganic fertilizer, as well as the used of appropriate variety. The field experiment was aimed to study the interaction of fertilizer application and the using of variety on rice. The research was conducted at April until July 2016 in Sekarputih Hamlet, Pendem Village, Junrejo District, Batu City. This research used the Randomized Complete Block Design (RCBD) Factorial methods with 2 factors are Rice Variety (V) and Fertilizers Doses (P) with 3 replications. Cows Biourine application by dissolved 1 L biourine with 10 L water sprayed on soil and plants. EM-4 application was doing by dissolved 100 cc EM-4 with 10 L water sprayed on soil. The result of research showed that interaction of Mapan-P.05 hybrid variety (V2) added with fertilizer doses 100 kg phonska+100 kg urea+cows biourine+EM-4 (P6) can increase yield on rice in parameter 1000 grain weight to 15.29% against which added fertilizer doses 200 kg phonska+200 kg urea (P1). Keywords: Inorganic Fertilizer, Organic Stimulant, Rice, Variety.

A Solid Waste Pond Tiger Shrimp (Peneaus monodon) as Fertilizer for Caulerpa lentillifera Nyoman Robby Manik Saputra; Sukoso Sukoso; Hartati Kartikaningsih
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Farming the shrimp, fish and another commodity could produce large quantities of waste. Aquaculture waste can be formed as feces, residual feed and dead organism which are accumulated in a cultivation area. Generally, the waste is discharged directly into the water without filtration. Thus, one of the problems that are often faced by farmer is the low quality of pure water due to the high content of nutrients. Moreover, suspended solid pollutant will be formed that lead to eutrophication, oxygen depletion, and precipitation. The aim of this research is to analyze the solid waste pond tiger shrimp as fertilizer for the growth of Caulerpa lentiilifera. Here, the Random Design complete (RAL) and three times in Deuteronomy are used in the experiment. The parameters of one control and three treatment doses are used 0, 2, 4, and 6 g.L-1 respectively. The results showed that solid waste shrimp ponds can be used as fertilizer to meet the needs of Caulerpa lentillifera for growth and the results show the highest value is found in the dose of 6 g.L-1 with NO3- (4.58 ppm), NH4+ (3.34 ppm), PO43- (2.03 ppm) and the value of the rate of growth and the PH are obtained (3.64 g.day-1) and (6,4-8), respectively. Keywords: Caulerpa lentillifera, growth rate, Nitrification, solid waste, Tiger Shrimp.

Phytochemical and Histochemical Screening of Toxic Plant Based on Knowledge of Tengger Tribe in Ngadiwono Village, Pasuruan Anggraeni In Oktavia; Jati Batoro; Serafinah Indriyani
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Around hundreds of plant's species have been confirmed to produce toxic that could endanger the life of human or domestic animals. Some of those plants have potentials to be developed as medicine. The objective of this study is to reveal the secondary metabolite content of toxic plants mentioned by Tengger tribe on Ngadiwono village through phytochemical and histochemical screening test. This study was conducted on Ngadiwono village, Tosari district, Pasuruan Regency. Informants were selected using snowball method by following the information of key person (n informant=14). Plants were selected and collected according to the local knowledge, then was analyzed qualitatively for its secondary metabolite content through phytochemical and histochemical screening test. The results showed that there are 8 plant species categorized as toxic by local people, including Bedor (Girardinia palmata), Kecubung Bunga Kuning (Brugmansia suaveolens), Kecubung Bunga Putih (Brugmansia suaveolens), Jarak (Ricinus communis), Terpasan Kuning (Cestrum elegans), Terpasan Merah (Cestrum elegans), Kudisan (Euphorbia pulcerrima), and Ciplukan (Physalis peruviana). The phytochemical result indicated that all toxic plants mentioned by the local people contain alkaloid substancess, while histochemical test showed that alkaloid substances were found in leaf trichomes, except in Terpasan Merah (Cestrum elegans). Keywords: Fitochemical, Histochemical, Tengger Tribe, Toxic Plant.

Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Fery Abdul Choliq; Tsang-Hai Chen; Liliek Sulistyowati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of 19.5 kDa as the size of coat protein of tobamoviruses was estimated by sodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigid rod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp. in Taiwan.Keywords: Frangipani plant, FrMV, mosaic disease, Tobamovirus.

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Home Page

OAI Link

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Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

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Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA