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All Journal The Journal of Experimental Life Sciences (JELS) Journal of Innovation and Applied Technology Research Journal of Life Science Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Pengabdian Magister Pendidikan IPA Journal of Aquaculture and Fish Health Journal Of Aquaculture Development and Environment

Agoes Soeprijanto, Agoes

Brawijaya University

Author-ID : 5918433

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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Profile of Amino Acid and Fatty Acid from Different Type of Worm as Feed of Broodstock Candidates for Domesticated Uceng (Nemacheilus fasciatus) Sholikah, Lik Anatus; Soeprijanto, Agoes; Kilawati, Yuni
Research Journal of Life Science Vol 7, No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.02.5

Uceng fish (Nemacheilus fasciatus) is a fish that lives wild on the river. All this time, the people have only relied on fishing from nature to consume, but the high market demand for fish will increase river fishing activities in excess. One of the steps to suppress the decline in fish populations is domestication because currently, the fish is not yet cultivated. The domestication process by feeding worms containing amino acids and fatty acids accelerates broodstock candidates' gonadal development. This research aims to determine the type of worms suitable as feed for the growth and gonadal development of broodstock candidates. The method that used in this research is Completely Randomized Design (CRD) with 3 treatments and 3 repetitions. Based on the analysis of amino acids and fatty acids in each type of worm, the best results were obtained on Lumbricus rubellus with an amino acid content of 50.91 gr/100gr and fatty acid 292.27%.

UTILIZATION OF ARTEMIA IN THE LAMINING OF CAT FISH, Clarias sp. IN KARANG BESUKI URBAN VILLAGE Agoes Soeprijanto; Pratama Deffi Samuel; Muhammad Fakhri
Journal of Innovation and Applied Technology Vol 3, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

AbstractThe main problem in catfish hatchery is low productivity and survival rate (SR). The aim of this work was to evaluate the application of artemia in catfish hatchery. Artemia was administered to 100,000 catfish larvae for 3 days with the total of 602 gr. The results showed that 70,000 catfish juveniles with size of 2-3 cm were obtained under artemia application. The survival rate of this study was 70%. It can concluded that probiotic application was able to improve the survival rate and the feed conversion ratio in catfish culture.

Application Of Vegetative Biofilter With Tricking Filter Technique On Water Supply With High Sulphur Content To Increase Seed Production Of Traditional Nile Tilapia Fish Hatchery In Tegal Randu Village, Klakah District, Lumajang. Agoes Soeprijanto
Journal of Innovation and Applied Technology Vol 1, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Tegal Randu Village, Klakah District has high potency of natural resources in the form of Ranu Klakah, where in this place there are Keramba Jaring Apung (KJA) to have business of conducting nile tilapia fish ± 300 slots. One of the obstaclesin this business is conducting nile tilapia fish seed supply which sus-pends from other area, in limited number and there is no guarantee of this quality. In Tegal Randu and its surrounding areas there is no business of nile tilapia fish hatchery. Furthermore, water irrigation of Ranu Klakah contains high rate of sulfur so its quite vulnerable to business of fish hatchery. The purposes of this program were a) to give an education, train about nila fish hatchery b) education, train in the field of biofilter technique to be able to repair and improve the water quality for the nila fish hatchery. c) management of ecofriendly fishery conducting and d) to create new employment. Method which is used in this program is Counselling, Training, Discussion and demonstration of filter in the form of vegetative biofilter with trickling filter. The Result of this activity are a) Biofilter Vegetative System and Biodrum Filter Trickling b) the increasing of society enthusiasm to business of fish conducting and nile tilapia fish hatchery by the construction of some pool. This activity drives society enthusiasm so it needs to be continued with further development activities.

DETERMINING THE OPTIMUM TEMPERATURE FOR GROWTH, FEED EFFICIENCY AND SURVIVAL OF DOMESTICATED INDONESIAN MAHSEER, Tor soro LARVAE Jojo Subagja; Emir Ma’arif Imanudin; Kurniawan Kurniawan; Agoes Soeprijanto; Yunita Maemunah
Indonesian Aquaculture Journal Vol 16, No 2 (2021): (December, 2021)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.16.2.2021.91-97

Temperature plays a pivotal role in the success of larvae production. Evaluation of the effects of different temperatures on growth, feed efficiency, and survival of domesticated Indonesian mahseer (Tor soro) larvae was carried out to determine an optimum rearing temperature. Five different temperature settings of 22°C, 24°C, 26°C, 28°C, and 30°C were used as the treatments arranged in triplicates, in indoor closed recirculating systems. The larvae were stocked in a rectangular glass tank with a stocking density of 150 larvae per tank and fed with a commercial feed (30% protein content) three times a day, about 10% per body weight over 61 days of observation. Measured parameters included the specific growth rate of total weight (SGRTW) and length (SGRTL), feed efficiency (FE), and survival rate (SR). The result showed that the treatments of different temperatures significantly affected the growth performance and feed efficiency of the larvae. The growth performance and feed efficiency of mahseer larva were much better at rearing temperature from 24°C to 28°C compared with those at 22°C and 30°C. There were no significant differences in SR in different temperatures tested. The SR was found to range from 86.44% to 100% in all treatments. Maintaining temperature ranging from 24°C to 28°C is the best rearing condition to achieve the optimum growth and feed efficiency of mahseer during the larval rearing period. The findings from this research could be set as a standard technique in larval rearing of Indonesian mahseer (Tor soro).

HIBRIDISASI INTERSPESIFIK TIGA SPESIES IKAN TOR (Tor soro, Tor douronensis, DAN Tor tambroides) SECARA RESIPROKAL PADA FASE LARVA Toni Yulian Kusmardani; Otong Zenal Arifin; Agoes Soeprijanto; Yunita Maimunah
Jurnal Riset Akuakultur Vol 16, No 1 (2021): (Maret, 2021)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Ikan Tor merupakan genus yang potensi dibudidayakan karena memiliki nilai ekonomis tinggi. Masalah yang ada adalah lambatnya pertumbuhan dan rendahnya tingkat sintasan benih ikan tersebut dalam jumlah yang memadai untuk pembudidaya. Hibridisasi atau persilangan merupakan suatu upaya untuk mendapatkan kombinasi antara populasi yang berbeda untuk menghasilkan keturunan yang memiliki sifat unggul. Hibridisasi tiga spesies ikan Tor secara resiprokal antara Tor soro (Ts), Tor douronensis (Td), dan Tor tambroides (Tt) dilakukan guna untuk mengetahui keberhasilan hibridisasi beda spesies tetapi masih dalam genus. Metode yang digunakan pada penelitian ini adalah eksperimen dengan enam perlakuan dan tiga kali ulangan hasil hibrida. Hasil yang diperoleh menunjukkan nilai derajat pembuahan tertinggi dihasilkan dari hibridisasi Td >< Ts (93,66 ± 1,15%), daya tetas tertinggi dihasilkan dari persilangan Td >< Ts (88,00 ± 1,00%), laju pertumbuhan spesifik bobot tertinggi dihasilkan dari hibridisasi Ts >< Td dan Ts >< Tt (5,62 ± 0,17%; 5,44 ± 0,06%), laju pertumbuhan spesifik panjang tertinggi dihasilkan dari hibridisasi Ts >< Td (1,79 ± 0,11%) dan sintasan tertinggi dihasilkan dari hibridisasi Tt >< Ts (86,00 ± 3,60%). Nilai heterosisyang meliputi derajat pembuahan, daya tetas telur, pertumbuhan benih, dan sintasan ikan sampai umur 41 hari menunjukkan nilai negatif, kecuali laju pertumbuhan spesifik bobot. Hibridisasi berdampak pada meningkatnya jumlah larva abnormal yang dihasilkan dibanding tetuanya (pure breed).Tor fish has the potential to be commercially developed as a farmed fish due to its high economic value and market demand. However, slow growth and low survival rate of the fish larvae and insufficient seed availability for aquaculture farmers are the main bottlenecks to develop a profitable aquaculture for tor fish. Hybridization or crossbreed is an attempt to get a combination between different populations to produce offspring inheriting superior characters. A reciprocal hybridization between Tor soro (Ts), Tor douronensis (Td), and Tor tambroides (Tt) was carried out to assess the success rate of hybridization between the three species. The study used an experimental design with six treatments and three replications to measure and compare hybridization and survival parameters between the species. The highest fertilization rate attained by hybridization Td >< Ts (93.66 ± 1.15%, the highest hatching rate was achieved by Td >< Ts (88.00 ± 1.00%), the highest weight specific growth rate was gained by hybridization Ts >< Td and Ts >< Tt (5.62 ± 0.17%; 5.44 ± 0.06%), the highest lenght specific growth rate was achieved by hybridization Ts >< Td (1.79 ± 0.11%) and highest survival rate was resulted from hybridization between Tor tambroides >< Tor tambroides (90.33 ± 1.52%). The observed heterosis (degree of fertilization rate, hatching rate, growth rate, and survival rate until the age of 41 days) showed that all parameter characters were negative, except for the weight-specific growth rate characters. This study concludes that hybridization have an impact on increasing the number of abnormal larvae produced compared to their parents (pure-breed).

Effectiveness of Using Pakcoy (Brassica rapa L.) and Kailan (Brassica oleracea) Plants as Vegetable Media for Aquaponic Culture of Tilapia (Oreochromis sp.) Heri Ariadi; Immanuel A.H. Pandaingan; Agoes Soeprijanto; Yunita Maemunah; Abdul Wafi
Journal of Aquaculture Development and Environment Vol 3, No 2 (2020): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v3i2.3320

Aquaponic is one way to use a narrow area of land as a medium for fish cultivation and vegetables in one compartment. The types of commodities that are often cultivated using this system are tilapia fish, pakcoy vegetables, and kailan plants. The purpose of this study was to assess the effectiveness the use of pakcoy (Brassica rapa L.) and kailan (Brassica oleracea) on growth rates and feed conversion ratios by tilapia fish (Oreochromis sp.) cultivated in aquaponic aquaculture systems. The method used was an experimental design with a completely randomized using 3 treatments and 3 replications. The main parameters analyzed in this study were the specific growth rate (SGR) and the fish feed conversion ratio (FCR). Meanwhile, the supporting parameters measured include water quality variables such as temperature, pH, dissolved oxygen, ammonia, nitrite and nitrate. The results showed, during the maintenance of tilapia fish (Oreochromis sp.) with an aquaponic system, pakcoy (Brassica rapa L.) and kailan plants (Brassica oleracea) had an effect on increasing growth rate and feed conversion ratio by tilapia (Oreochromis sp.). In addition, during the maintenance period the water quality parameters also tend to be stabilized and comply with quality standards. The conclusion from this research is that the application of kalian plant (Brassica oleracea) use in the aquaponic system of tilapia commodities is more effective than pakcoy plants (Brassica rapa L.) with an effective SGR value at 3.15% and an FCR value at 1.21.

The Mitogen-Activated Protein Kinase (MAPKs) Expression and Primary Stress Hormone (Cortisol) of Clarias sp. Juvenile in Different Light Intensity Agoes Soeprijanto; R Adharyan Islamy
Journal of Aquaculture and Fish Health Vol. 12 No. 1 (2023): JAFH Vol. 12 No. 1 February 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i1.35054

Clarias sp. is one of major genera of freshwater catfish and also common commodity in tropical aquaculture. Excessive light intensity is expected to be responded to by fish as environmental stressors. Fish that experience stress will increase the secretion of catecholamine, cortisol, blood glucose levels and p38 MAPKs in catfish. The different intensity of lights will affect the fish physiological processes. The purpose of this study was to analyze physiological responses (Level of blood glucose, primary stress hormone (Cortisol) and p38 MAPKs) catfish Clarias sp. which were incubated at different lights intensity of catfish. 300 fish were collected from Kepanjen Regency, East Java. Blood and meat sampling are carried out to test the profile of glucose, cortisol, and p38 MAPKs. The experimental research at this stage were carried out by transferred the catfish from Kepanjen to experimental aquarium (50 x 30 x 30 cm) that setted the different lights intensity (0 lux (control), 400 lux, 800 lux and 1200 lux). Each experimental aquarium is filled with 15 catfish and will be kept it for 21 days. Quantificationof the parameter using ELISA method. The study result showed highest peak of primary stress hormone (cortisol) levels are 34±1,50 ng/mL, blood glucose levels are 120±3,4 mg/dL, and p38 MAPK are 97±1,1 %. All of it were achieved in the treatment of 1200 lux on the 2nd week.

The Quality of Ornamental Japanese Carp (Cyprinus carpio var. Koi) After Electroporation as a Gene Material Transfer Method Saidah Luthfiyah; Agoes Soeprijanto; Yuni Kilawati
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Spermatozoa are male gametes that have an advantage in acting as gene transfer media (SMGT), because in transferring genetic material, sperm uses relatively natural vectors. This study was conducted to know sperm quality of Koi after short electroporation with different voltages using Gene Pulser. The study was conducted using a complete randomized design (RAL) with three (3) replications and five (5) treatments, namely: 10 V.cm-1, 20 V.cm-1, 30 V.cm-1, 40 V.cm-1, and 1 control. Electroporation was done with square wave, 1 ms duration of electroporation, 4 times of electroporation shocks with 0.1 s duration. The results showed that electroporation with different voltage levels significantly affected the motility and viability of sperm. Based on orthogonal polynomial analysis, it was known that the treatment affected motility and viability of sperm with a linear equation y = -0.9x + 5, 4 R² = 0.920 and r = 0.96 for motility and y = -11.26x + 91.61 R² = 0.823 and r = 0.91 for viability, meaning that the higher the level of electroporation the lower the viability and sperm motility produced. From the results of this study, it can be suggested that the electroporation of Koi sperm as a gene transfer medium should be performed at a voltage of 10 V.cm-1. Keywords: electroporation, Koi, sperm quality, transgenesis.

The Effect of Electroporation Method towards the Motility and Viability of Java Barb Fish (Puntius javanicus) Sperm Dimas Adetia Rikianto; Agoes Soeprijanto; Yuni Kilawati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Electric shock treatment of Java Barb Fish (Puntius javanicus) sperm using electroporation method on sperm as transfer gen (Sperm Mediated Gen Transfer) has not been implemented in Indonesia. This study was conducted to know the effect of electric shock using gene pulser at different voltage level toward motility and viability of Java barb fish sperm. This research was conducted at Fish Breeding Laboratory, Faculty of Marine and Fisheries and LSIH of Brawijaya University-Malang in May 2017. The trial design used Complete Randomized Design with 3 different treatments and 1 control which each treatment repeated 3 times. The treatments used the Gene pulser with 3 different voltages: A (20 V), B (30 V), C (40 V). The result showed that the electric shock treatment with different voltages level affected motility and viability of Java barb fish sperm. Based on the data analysis used polynomial orthogonal, a linear-form of the relationship among the treatments in the form of equation was found (Puntius javanicus) with R2=0.9815 and equation y = -40.5x + 130.83 with R2= 0.8626. Based on the result of this research, electroporation for Java barb fish' sperm as gene transfer media should be done with voltage 20 V, pulse number 4 times and pulse length 1 ms. Keywords: electroporation, motility, Puntius javanicus, sperm, viability.

Effect of 17β-Estradiol on Feminization, Growth Rate and Survival Rate of Pacific White Shrimp (Litopenaeus vannamei, Boone 1931) Postlarvae I Nengah Gde Sugestya; Maheno Sri Widodo; Agoes Soeprijanto
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

This study was therefore aimed to determine the effect of different concentration of estrogen hormone, 17β-estradiol (E2) on feminization for the production of all female, growth rate and survival rate of Pacific white shrimp (Litopenaeus vannamei) postlarvae (PL). L. vannamei PL1 were stocked randomly at a density of 10 ind.L-1 into a 10 L of seawater for each experimental flask with three replicates each. PL1 was immersed in seawater containing 0.5, 1.0 and 1.5 mg.L-1 estrogen hormone, E2 for 4 hours. The experiment was continued for 30 days for larval rearing. At the termination of experiment, the specimens in each treatment groups were weighed and measured individually for their wet body weight (BW) and total length (TL) for the mean weight gain, length gain, and the specific growth rate (SGR) estimation. The mean sex ratio values of male to female postlarvae obtained from control till the highest E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 1:1, 1:2, 1:9 and 0:1 respectively. The weight gain and length gain were 40605% and 606% for control, 46310% and 647% for 0.5 mg.L-1 concentration, 49310% and 663% for 1.0 mg.L-1 concentration and 45048% and 628% for 1.5 mg.L-1. The mean SGR BW and SGR TL for control till the highest E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 20.00 and 6.51, 20.46 and 6.70, 20.67 and 6.77 and 20.37 and 6.62 respectively. The mean survival rate for E2 hormone concentration of 0, 0.5, 1.0 and 1.5 mg.L-1 were 52.67%, 54.33%, 54.67% and 52.33% respectively. The study shows that while the concentration of E2 hormone increased, the female sex ratio of L. vannamei PL also increased but the growth rate and survival rate decreased at concentration of 1.5 mg.L-1. Thus the optimum concentration of this hormone usage was 1.0 mg.L-1 immersed for 4 hours. Keywords: 17β-estradiol, feminization, growth rate, Litopenaeus vannamei, postlarvae, survival rate.

Co-Authors Abdul Wafi Aisyah, Diana Diana Aisyah Dimas Adetia Rikianto Emir Ma’arif Imanudin Harun Wijaya Heri Ariadi I Nengah Gde Sugestya Immanuel A.H. Pandaingan Jamroni, Mofit Jojo Subagja Kurniawan Kurniawan Maheno Sri Widodo, Maheno Sri Muhammad Fakhri Otong Zenal Arifin Pratama Deffi Samuel R Adharyan Islamy Saidah Luthfiyah Sholikah, Lik Anatus Subandi, Hendi Supriyadi Supriyadi Toni Yulian Kusmardani Yuni Kilawati Yunita Maemunah Yunita Maemunah Yunita Maimunah

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