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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 10 Documents

Search results for , issue "Vol. 8 No. 2 (2018)" : 10 Documents clear

The Effect of E. scaber Extract to TNF-α and TGF-β on Mice under Carcinogen Treatment Rizki Amalia; Sri Widyarti; Moch. Sasmito Djati
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Carcinogen compounds are the compounds that cause DNA damage and trigger cancer. TNF-α and TGF-β are cytokines produced by immune cells and serve to maintain body homeostasis. Elephantopus scaber is a plant that prevents the cancer progression, and improve the body's immune system, so this study was conducted to determine the effect of E. scaber extract on TNF-α and TGF-β after the administration of carcinogen compounds. The study was conducted by administering the carcinogen compound DMBA with a dosage of 0.56 mg kg-1 BW and estradiol with a dose of 0.0504 mg kg-1 BW which was given alternately for 8 weeks to the Mus musculus test animals. The study was conducted in 3 groups: K- (the normal group of mice), K + (a carcinogen-induced group of mice), and P (a group of carcinogen-induced mice and E. scaber extract). The treatment was done in 1 week, 2 weeks, and 3 weeks. Immune cells were isolated from splenocytes and performed immunostaining for flow cytometry analysis. The computed relative amount is TNF-α produced by macrophages CD11b and TGF-β produced by Treg CD4CD25. The relative number of cells was analyzed by two-way ANOVA and advanced Tukey test with 95% confidence level (α = 0.05). The results showed no significant differences in the number of cytokines TNF-α and TGF-β in both the carcinogen-induced mice group and the mice group was given the extract of Elephantopus scaber for 1 week, 2 weeks and 3 weeks. Keywords: Carcinogen, Elephantopus scaber, TNF-α, TGF-β

Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum Nurul Mutmainnah; Yenny Risjani; Asus Maizar Suryanto Hertika
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Porphyridium cruentum microalgae have great potential to be developed as a source of active ingredients in various fields of health. It excretes secondary metabolites in the form of extracellular polysaccharides (EPS), potentially as antibacterial, antihyperglycemic, and immunostimulant components. This study aims to obtain the best culture density of P. cruentum, the active component of P. cruentum (EPS), and the chemical composition of EPS. The P. cruentum was cultivated on 15%, 20% and 25% culture stock, with an addition of silicate, Fe and vitamins in sterile seawater medium with salinity 35 ‰, pH 8, temperature 25-27°C, with continuous aeration and 2500 lux continuous 24 hours, for 14 days of culture period. The results of this study indicate differences in growth rates and abundance of microalgae to each culture stock. The 15%, 20% and 25% culture stocks each showed stationary phase at days 10th, 12th and 14th, with EPS concentrations of 10 ppt, 12 ppt, and 15 ppt, respectively. Variation of P. cruentum culture showed different EPS results, with a density of 25% capable of producing the highest EPS extract of 15,000 mg.L-1. The EPS is known to contain glucose and carboxylic acid compounds that can be utilized in the health and industrial fields. Keywords: culture, extracellular polysaccharides, Porphyridium cruentum.

Patellar Giant-Cell Tumor: a Case Report Istan Irmansyah Irsan; Satria Pandu Persada; Rakhmad Aditya
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Giant cell tumor (GCT) found mostly in the long bones metaphysis or epiphysis. GCT usually occur between the ages of a third and fourth decade and locally aggressive. Giant-Cell Tumor seldom affects the patella. Multicentric forms rarely reported. A fourteen-year-old female with a lump at her left patella since one-year associated with slight pain that aggravated by activity for six months. Plain X-ray left knee AP, and lateral views reveal expansile lytic lesion in left patella with thinning of the anteroinferior cortex and sclerotic septa within. MRI of left knee shows approximately 3x2x2 cm heterogeneous lobulated expansile soft tissue mass in left patella extending up to the patellofemoral joint with fluid-fluid appearance. From fine needle aspiration cytology, resulting giant-cell tumor with the differential diagnosis of an aneurysmal bone cyst. The operation already performed. Curettage, bone graft, and a biopsy taken. An immunocytochemical smear was performed and confirmed as a patellar giant-cell tumor. Six months after excision of the tumor, the patient complained no arthralgia and have a full range of motion for the knee. Keywords: Giant-cell tumor, knee lump, knee pain, patella

The Alteration Tissue Structure of Digestive diverticulum of Anodonta woodiana Induced by Lead Renanda Baghaz Dzulhamdhani Surya Putra; Sri Andayani; Hartati Kartika Ningsih; Diana Arfiati; Asus Maizar Suryanto Hertika
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Accumulated the toxic compound such as lead in A. woodiana caused an alteration in tissue. The aim of the present study is observation through histology to determine the alteration of the structure of digestive diverticulum tissue. A. woodiana were maintained in water tub that treated lead II nitrate exposure (Pb(NO3)2) 0, 15, 25, 35 mgL-1 for 7 days to determine the alteration of the structure of damaged the digestive diverticulum and determining water quality such temperature, DO and pH. The highest damage occurs in digestive diverticulum treated 35 mgL-1 due to the many alteration tissue structure such as hyperplasia, edema, atrophy, necrosis with the percentage of total damage of about 35%. the lowest alteration tissue structure occurs in 15 mgL-1 treatment group of the digestive diverticulum. the temperature was observed at about 25.3 - 26.4°C, pH about 6-7, and DO about 6.1-6.7 mgL-1. Thus, the increment of lead dose treatment induces increment of tissue structure alteration in digestive diverticula tissue, therefore, might promote the death of Anodonta woodiana. Keywords: Anodonta woodiana, digestive diverticulum, histology

Biological Analysis of Blue Swimmer Crab (Portunus pelagicus) from Collectors in Lamongan, Tuban, Pasuruan, and Rembang, Java, Indonesia Lini Murni; Diana Arfiati; Andi Kurniawan; Yuni Kilawati; Herwati Umi Subarijanti; Suliswanto Suliswanto; Muhammad Nafar Amani Syams; Agum Bayu Gumelar; Alif Raditya Amirul Huda
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Blue swimmer crab (Portunus pelagicus) can increase the country's incomes due to its high demand of export. This study aims to determine biological analysis on the population of blue swimmer crab in Lamongan, Tuban, Pasuruan, and Rembang. The sampling location was based on the fishing area and the method used survey method from collectors in each location. The blue swimmer crab's biological characteristics which observed are the correlation on wide of carapace and blue swimmer crab weight, sex ratio, and gonad maturity level. The results showed the correlation between the wide and weight of carapace in Lamongan (male: 113.80-122.99 mm and 87.98-121.60 g then female: 108.21-117.27 mm and 71.80-99.74 g), Tuban (male: 113.26-117.74 mm and 89.63-102.76 g g then female: 114.36 mm and 106.51 g), Pasuruan (male: 87.66-94.43 mm and 39.68-49.90 g then female: 101.94-110.89 mm and 66.98-82.89 g), and Rembang (male: 120.30-126.69 mm and 93.53-118.43 g then female: 125.30-132.13 mm and 134.42-157.47 g); sex ratio (male: female) in Lamongan (1.4: 1), Tuban (1: 1.5), Pasuruan (2.8: 1), and Rembang (1: 1.46); and also gonad maturity level in Lamongan (dominant male at level III was 30.29%, female at level II and III was 30.40%), Tuban (dominant male at level II was 41.93%, female at level III was 33.33%), Pasuruan (dominant male at level III was 25.79%, female at level III was 48.10%), and Rembang (dominant male at level II was 32.35%, female at level II was 33.33%). These results indicate that the total of Portunus pelagicus which was being caught had fulfilled the standards rules by No.1/PERMEN-KP/2015. However, it requires continuous direct monitoring and counseling to maintain the stock of blue swimmer crab resources. Keywords: Blue swimmer crab (Portunus pelagicus), gonad maturity level, sex ratio, wide and weight.

Rosella Flower Extract Prevent Increasing of Interleukin-6 and Amyloid-β Levels in Brain Tissue of Heated Diet-Treated Rats Ardhiyanti Puspita Ratna; Setyawati Soeharto; Edi widjajanto; Silvy Amalia Falyani; Pia Bataif Batmomolin
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Heated food is a source of formation of various types of Advanced Glycation Endproducts (AGEs). One of which is N-carboxymethyl-lysine (CML) that is often used as a sign of the formation of AGEs in the diet. The accumulation of AGEs in the brain can trigger neurodegenerative conditions. It also accelerates the accumulation of Amyloid β and proinflammatory cytokines which exacerbate neurodegeneration. Hibiscus sabdariffa L or rosella known to have antiglycation, antioxidant, and anti-inflammatory effects. This study aims to prove the effect of food heating to the increasing levels of CML serum and evaluate the effects of daily intake of Rosella extract IL-6 levels and Amyloid β in the Wistar rats' brain given a diet high of AGEs. This experimental study with post test only control group design was conducted using 25 white male rats Wistar strain (Rattus novergicus) which were divided into 5 groups namely positive control, negative control, the treatment group with rosella dose 200mg.kgBW-1, 300mg.kgBW-1, and 400mg.kgBW-1. The high diets of AGEs are given for 12 weeks, and it was added to the rosella extract with various doses on the 9th to 12th weeks. The examination of CML serum level, interleukin 6 brain level, and Amyloid β of the brain was carried by ELISA. This study found differences between CML serum level of rats which fed a standard diet and the one that was given a heated diet (p= 0.0001). It also found that rosella flower extract can prevent the increasing levels of interleukin 6 and Amyloid β in the brain tissue of rats which were given a heated diet, and both effective doses were on 200mg.kgBW-1. Keywords: Advanced Glycation End products, Amyloid β, Interleukin 6, N-carboxymethyl-lysine, Rosella.

Dynamical Analysis of Predator-Prey Model Leslie-Gower with Omnivore Rina Exviani; Wuryansari Muharini Kusumawinahyu; Noor Hidayat
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

This article discussed a dynamical analysis on a model of predator-prey Leslie-Gower with omnivores which is modified by Lotka-Volterra model with omnivore. The dynamical analysis is done by determining the equilibrium point with its existing condition and analyzing the local stability of the equilibrium point. Based on the analysis, there are seven points of equilibrium. Three of them always exist while the four others exist under certain conditions. Four points of equilibrium, namely andÂ are unstable, while the others three equilibrium point are local asymptotically stable under certain conditions. Moreover, it's also conducted numerical simulations to illustrate the analytical. The results of numerical simulations agree with the results of the dynamical analysis. Keywords: local stability, omnivore, predator-prey models, the equilibrium point

Heavy Metal Copper (Cu) Distribution in Water and Its Bioaccumulation in Green Mussel (Perna viridis) in Coastal Area of Ujung Pangkah, Gresik District Mohammad Qodri Fitra; Diana Arfiati; Bambang Semedi
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Environmental pollution in the form of increased concentration of heavy metal Copper (Cu) becomes the main factor that can affect water quality and inhibit green mussel culture (Perna viridis) in Ujung Pangkah Coastal Area of Gresik District. The purpose of this research is to analyze concentration of heavy metal Copper (Cu) on water and its bioaccumulation in green mussel (P. viridis) in Ujung Pangkah waters, Gresik District. This research will be conducted from June to August 2017. The method used in this research is descriptive method. Sampling of seawater and mussel by purposive sampling. Cu heavy metal content in Ujung Pangkah waters, Gresik District has 0.003 - 0.006 ppm in July and 0.002 - 0.006 ppm in August. That value is still safe below predetermined quality standards. The results of heavy metal measurement test in shellfish has 0.159 to 0.69 ppm in July and 0.162 - 0.71 ppm in August. The high concentrations of Cu heavy metals in Ujung Pangkah waters are simultaneous to the bioaccumulation concentration in the green mussel. The higher concentration in the waters, the higher the bioaccumulation in the green mussels. And vice versa. The distribution of Cu heavy metal concentration at point 4 is the highest. While at point 1 has the lowest Cu heavy metal concentration. Keywords: Copper, Bioaccumulation, Green Mussel, Heavy Metal, Perna viridis.

Geographically and Temporally Weighted Regression Modeling in Analyzing Factors Affecting the Spread of Dengue Fever in Malang Fahmi Indrayani; Henny Pramoedyo; Atiek Iriany
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Geographically and Temporally weighted regression (GTWR) modeling has been developed to evaluate spatial heterogeneity and temporal heterogeneity in factors influencing the spread of dengue fever in Malang city. By using the monthly data in 2012-2015 as the temporal unit of each urban village in Malang and village is considered as a spatial unit. GTWR model is compared with the GWR model using several statistical criteria. GTWR model shows that the relationship between dengue incidence with population density and monthly average temperature significantly affects each Village in Malang.Keywords : DHF, GTWR, Spatiotemporal Pattern

Optimal Control of Tumor Growth Model with Dendritic Cells as Immunotherapy Firmansyah Reskal Motulo; Trisilowati Trisilowati; Abdul Rouf
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

In this paper, optimal control of tumor growth model with dendritic cells as immunotherapy is provided. The model equation can be expressed into a nonlinear differential equation system consisting of four compartments namely, tumor cells, CTL cells, helper T cells, and dendritic cells. Dendritic cells as immunotherapy are injected to the body at time t. The aim of this optimal control is to minimize the tumor cells density as well as the cost of dendritic cells to be administered to the body.Optimal control problem is carried out based on Pontryagin's maximum principle and numerical simulation is solved by using Forward-Backward Sweep methods. Simulation results show that control strategy shrinks tumor cells density which is shown by tumor cells density graph that monotonically decreases after applying dendritic cells as immunotherapy.Keywords: immunotherapy, optimal control, Tumor cell.

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Jehan Ramdani Hariyati

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Jawa timur

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

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Abstract

Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA