The Journal of Experimental Life Sciences (JELS)
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Articles
10 Documents
Search results for
, issue
"Vol. 9 No. 1 (2019)"
:
10 Documents
clear
<![CDATA[Sensitivity and Stability Analysis of a SEIR Epidemic Model with Information ]]>
<![CDATA[Robiatul Witari Wilda]]>;
<![CDATA[Trisilowati Trisilowati]]>;
<![CDATA[Moch. Aruman Imron]]>
<![CDATA[ The Journal of Experimental Life Science Vol. 9 No. 1 (2019) ]]>
Publisher : <![CDATA[Postgraduate School, Universitas Brawijaya ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (1163.176 KB)
|
DOI: 10.21776/ub.jels.2019.009.01.08
<![CDATA[In this paper, the construction and stability analysis of a SEIR epidemic model with information are discussed. This model contains information about how to prevent the spread of infectious diseases which is transmitted by infected individuals to susceptible individuals. Furthermore, the dynamical analysis of the model which includes determination of equilibrium points terms of existence, stability analysis of the equilibrium points and sensitivity analysis are observed. Local stability of the equilibrium point is determined by linearizing the system around the equilibrium point and checking for the eigenvalue sign of Jacobian matrix at each equilibrium point. Sensitivity analysis is performed by using a sensitivity index to measure the relative change of basic reproduction number on each parameter. Based on the analysis result, there are two equilibrium points namely disease-free equilibrium point and endemic equilibrium point. The disease-free equilibrium point always exists and is locally asymptotically stable if the basic reproduction number is less than one. Moreover, the endemic equilibrium point exists and is locally asymptotically stable under certain conditions. From sensitivity analysis, it is found that the rate of mortality is the most sensitive parameter and the least sensitive parameter is the rate of exposed individual becomes infected individual. Finally, numerical simulation is conducted to support the analysis result.Keywords: Epidemic, information, sensitivity analysis, SEIR, stability analysis.]]>
<![CDATA[Phytoremediation of Lead-Contaminated Soil by Using Vetiver Grass (Vetiveria zizanioides L.) ]]>
<![CDATA[Rawidh Said Abdallah]]>;
<![CDATA[Arief Rachmansyah]]>;
<![CDATA[Bagyo Yanuwiadi]]>
<![CDATA[ The Journal of Experimental Life Science Vol. 9 No. 1 (2019) ]]>
Publisher : <![CDATA[Postgraduate School, Universitas Brawijaya ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (1367.357 KB)
|
DOI: 10.21776/ub.jels.2019.009.01.09
<![CDATA[Lead become a frequent problem in the environment, especially in the soil. However, there are plenty of plant species that can be used to overcome that problem. This study was aimed to analyze the effectiveness of Vetiver grass to reduce hyper-accumulate heavy metal such as lead (Pb) in the soil. Moreover, this study focused on the determination of heavy metals in the soil before plantation and after plantation of Vetiver. The method used was by planting the Vetiver grass in soil that was already treated by lead nitrate, lead chloride, and lead sulfate. Samples were collected from these treated soil every two weeks and samples of the vetiver grass were taken after one week for ten weeks. The result shows that Vetiver grasses are able to remove hyper-accumulate lead from the treated soil.Key words: Heavy metals, hyper accumulator, Vetiver grass.]]>
<![CDATA[Role of Active Compounds of Bohadschia argus Inhibit Cancer Cell Survival ]]>
<![CDATA[Jantje Wiliem Souhaly]]>;
<![CDATA[Sri Rahayu]]>;
<![CDATA[Widodo Widodo]]>
<![CDATA[ The Journal of Experimental Life Science Vol. 9 No. 1 (2019) ]]>
Publisher : <![CDATA[Postgraduate School, Universitas Brawijaya ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (1142.888 KB)
|
DOI: 10.21776/ub.jels.2019.009.01.03
<![CDATA[Sea cucumber is marine biota with a high economic value and also has potential for anti-cancer. The purpose of this study was to explore the mechanism of active compound of Bohadschia argus on regulating cancer cell survival. The B. argus samples were collected from the sea of Kamal Village, West Seram Maluku, then extracted by water. The constituents of water extract of B. argus were examined by LC-MS. The network among active compound and its protein target were determined by Cytoscape app. The result shows that B. argus has several active compounds, such as chondroitin sulfate, holothurin A, holothurin B, and scabraside that might play a role in cancer cell apoptosis, proliferation, and metastasis. Keywords: Active compound, Bohadschia argus, LC-MS.]]>
<![CDATA[Antifeedant Effect of Moringa oleifera (L.) Leaf and Seed Extract on Growth and Feeding Activity of Spodoptera litura (Fab.) (Lepidoptera: Noctuidae) ]]>
<![CDATA[Amelia Tridiptasari]]>;
<![CDATA[Amin Setyo Leksono]]>;
<![CDATA[Dian Siswanto]]>
<![CDATA[ The Journal of Experimental Life Science Vol. 9 No. 1 (2019) ]]>
Publisher : <![CDATA[Postgraduate School, Universitas Brawijaya ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (1061.918 KB)
|
DOI: 10.21776/ub.jels.2019.009.01.05
<![CDATA[This study was conducted to evaluate the effect of Moringa oleifera leaf and seed extract on length, weight, and feeding activity of Spodoptera litura. Leaf and seed were extracted by maceration method in 70% ethanol. Bioassays were conducted by using five concentrations (0%, 5%, 10%, 15%, 20%) on 5 larvae by leaf-dip method with 5 times replication. The result showed that leaf and seed of Moringa extract contained alkaloid, flavonoid, and saponin which gave antifeedant effect to growth and feeding activity of larvae. The increased extract concentrations are lead to weight loss and the length shrinkage of larvae. The extracts promoted prolongation of the larval instar. Reduction of food intake of the larva is in line with the concentration increase of extracts. The antifeedant effect also affects time to stop feeding and give mortality of larvae.Keywords: antifeedant, leaf, moringa, seed, S. litura]]>
<![CDATA[The Effect of Difference Temperature on Cortisol, Glucose and Glycogen level of Uceng Fish (Nemacheilus fasciatus) ]]>
<![CDATA[Harun Wijaya]]>;
<![CDATA[Maheno Sri Widodo]]>;
<![CDATA[Agoes Soeprijanto]]>
<![CDATA[ The Journal of Experimental Life Science Vol. 9 No. 1 (2019) ]]>
Publisher : <![CDATA[Postgraduate School, Universitas Brawijaya ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (557.651 KB)
|
DOI: 10.21776/ub.jels.2019.009.01.01
<![CDATA[Nemacheilus is one of the genera of freshwater fish. The existence of Uceng fish (Nemacheilus fasciatus) in public waters is increasingly rare. Changes in water temperature in the maintenance medium will affect the physiological processes of fish. The purpose of this study was to analyze physiological responses (blood glucose levels, cortisol, and glycogen hormones) of Uceng Fish (N. fasciatus) which were incubated at different temperatures and find out the initial time of Uceng fish adaptation to its new environment. Total of 200 fish were collected from wild catches in the Lekso River, Blitar. Fish tissue collection was carried out to test the profile of glucose, cortisol, and glycogen. The research activities at this stage were carried out by raising the Uceng fish from nature to a cultivation container in an aquarium of size 50 x 30 x 30 cm that given the different temperatures (20°C, 24°C, 28°C and 32°C), and each aquarium is filled with 10 Uceng fish and will be kept for 14 days. ELISA method was used to quantify the parameter. The result of this study showed highest peak of cortisol levels and blood glucose levels was achieved in the treatment of 24°C on the 10th day and the lowest in the treatment temperature of 28°C on the 6th day. The highest glycogen peak was reached at 32°C on day 10 and the lowest peak was reached at 28°C on day 2. We conclude that temperature affects the physiological response (cortisol, blood glucose, and glycogen) of Uceng fish (N. fasciatus). High levels of cortisol and blood glucose indicate stressful fish. Keywords: Fish, Nemacheilus fasciatus, Physiological, Temperature, Uceng.]]>
<![CDATA[Protein Structure and Function Analysis Method of Aminoacyl-tRNA Synthetase Cofactor and Biotinylation Effect: Journal Review ]]>
<![CDATA[Restu Nugraha]]>;
<![CDATA[Chien Chia Wang]]>;
<![CDATA[Widodo Widodo]]>
<![CDATA[ The Journal of Experimental Life Science Vol. 9 No. 1 (2019) ]]>
Publisher : <![CDATA[Postgraduate School, Universitas Brawijaya ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (1083.411 KB)
|
DOI: 10.21776/ub.jels.2019.009.01.10
<![CDATA[Protein has been known as an important macromolecule which has a vital role among the living organism. One of the most interesting protein is Arc1p, which is a yeast-specific tRNA-binding protein. Arc1p is a unique protein that has the ability to form a ternary complex with glutamyl-tRNA synthetase (GluRSc) and methionyl-tRNA synthetase (MetRS) in the cytoplasm. This complex can significantly enhance the aminoacylation efficiency of these two aaRSs to their respective cognate tRNAs. Recently, it was found that Arc1p can be biotinylated via post-translational modification at Lys86 (K86) in the N-domain. Here, we try to figure it out what kind of method that will help to create some clear information both in structure and function of this protein, when mutations occur inside of the K86 site within SSKD motifs of Arc1p. Several methods to better understanding obviously about protein characteristics comprises protein structural analysis; such as gel mobility shift assay, CD Spectroscopy, and limited proteolysis; protein functional analysis, and in silico modeling.Keywords: Arc1p, biotinylation, function, in silico, structure.]]>
<![CDATA[Transoceanic Disperse of the White-lipped Island Pit Viper (Cryptelytrops insularis; Kramer,1997) from Sundaland to Lesser Sunda, Indonesia ]]>
<![CDATA[Bagus Priambodo]]>;
<![CDATA[Fu-Guo Robert Liu]]>;
<![CDATA[Nia Kurniawan]]>
<![CDATA[ The Journal of Experimental Life Science Vol. 9 No. 1 (2019) ]]>
Publisher : <![CDATA[Postgraduate School, Universitas Brawijaya ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (1141.019 KB)
|
DOI: 10.21776/ub.jels.2019.009.01.02
<![CDATA[White-lipped island pit viper (Cryptelytrops insularis) is one of the most distributed Viperidae in Indonesia, especially in eastern part of Sundaland and Lesser Sunda. To investigate the evolutionary history and the dispersal pattern of C. insularis, we collected 23 samples from 11 localities. Four simultaneous genes composing two mitochondrial genes (16S rRNA & ND4) and two nuclear genes (7IβFIB & 3ITBP) have been successfully amplified and sequenced. Bayesian inference was performed to reconstruct the phylogeny tree. Furthermore, time divergence and the population demography analyses were estimated. The phylogeny tree of C. insularis exhibits monophyletic group, with four geographically structured lineages. The time divergence estimation indicated that C. insularis evolved at approximately 7 million years ago (mya). Population demography was inferred by Bayesian Skyline Plot analysis, it shows that the population increased constantly from the past to recent time. The evolutionary history of C. insularis can be explained by a pattern of the time divergences estimation that indicating movement from West (Java) to East (Lesser Sunda). We expected that the dispersal factor of C. insularis into many different islands (in Lesser Sunda) is caused by the animal helped and also oceanic rafting which could be the stepping stones to another island. Keywords: Cryptelytrops insularis, dispersal patterns, phylogeny, population demography, time divergence]]>
<![CDATA[Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam ]]>
<![CDATA[Fitri Sil Valen]]>;
<![CDATA[Maheno Sri Widodo]]>;
<![CDATA[Yuni Kilawati]]>
<![CDATA[ The Journal of Experimental Life Science Vol. 9 No. 1 (2019) ]]>
Publisher : <![CDATA[Postgraduate School, Universitas Brawijaya ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (1080.646 KB)
|
DOI: 10.21776/ub.jels.2019.009.01.07
<![CDATA[In general, the process of naming several species of fish including species from the genus Mystacoleucus is still carried out based on morphological characters, which are often found in almost the same morphological characters in different fish species. Therefore, it is necessary to apply a more accurate identification method, which is a DNA-based identification method called DNA barcoding. The purpose of this study was to identify the species of the genus Mystacoleucus on the Sengguruh Dam molecularly based on the Cytochrome Oxidase C Subunit I (COI) gene. A caudal fin was taken on the test fish and preserved in 95% ethanol solution for molecular identification. The results of the identification showed that the sample belonged to the Mystacoleucus marginatus species with Identity values between 99-100% and E-value 0.0. The data obtained showed that from the calculation of genetic distance presented in the form of data matrix and phylogenetic tree reconstruction, there were 2 species that had a far genetic distance from the M. marginatus sample from the Sengguruh Dam namely M. atridorsalis with the furthest genetic distance of 0.1932-0.2595 and M. lepturus with genetic distance between 0.1117-0.1193. However, there are one species that has the closest distance, M. padangensis with genetic distance between 0.0019-0.0038 and identity values up to 99%.Keywords: DNA barcode, Molecular identification, Mystacoleucus, Phylogenetic COI.]]>
<![CDATA[Anatomical Characters of Shoot Apical Meristem (SAM) on Bulbil Porang (Amorphophallus muelleri Blume) At the End of Dormancy Period ]]>
<![CDATA[Muhammad Naufal Afifi]]>;
<![CDATA[Nunung Harijati]]>;
<![CDATA[Retno Mastuti]]>
<![CDATA[ The Journal of Experimental Life Science Vol. 9 No. 1 (2019) ]]>
Publisher : <![CDATA[Postgraduate School, Universitas Brawijaya ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (1095.941 KB)
|
DOI: 10.21776/ub.jels.2019.009.01.04
<![CDATA[Porang is one species member of the genus Amorphophallus. In Indonesia, Porang is cultivated extensively because it has a high economic value. There is three seeded material available for planting. One of seeded material is bulbil. Bulbil is a vegetative reproductive organ of Porang plants which is located in the branch of the leaf. Bulbil has 3 - 4 months dormancy period. The dormancy period will end with shoot growth. Morphologically, there can be found a rough structure in the form of bulges in bulbil. The bulge is spread throughout the bulbil surface and is a growing area of shoots. Shoots on bulbil will grow through the bulge and only grow on the adaxial part, contrary shoots do not grow from the abaxial part. This study aims to compare the anatomy of shoot apical meristem (SAM) in the adaxial and abaxial parts of bulbil at the end of the dormancy period, ie 100 days after harvest (DAH), 110 DAH, 120 DAH, and 130 DAH. Anatomical observations were carried out by making semi-permanent preparations. The results showed that the anatomy of the adaxial and abaxial parts of bulbil Porang had differences in the development of SAM, leaf primordia, primary thickening meristem (PTM) and procambium.Keywords: Anatomy, Bulbil Porang, Dormancy, Shoot Apical Meristem.]]>
<![CDATA[Effect of Place and Time Storage on the Quality of Tubers Amorphophallus muelleri Blume ]]>
<![CDATA[Putri Rabiah Al Adawiah]]>;
<![CDATA[Rodiyati Azrianingsih]]>;
<![CDATA[Retno Mastuti]]>
<![CDATA[ The Journal of Experimental Life Science Vol. 9 No. 1 (2019) ]]>
Publisher : <![CDATA[Postgraduate School, Universitas Brawijaya ]]>
Show Abstract
|
Download Original
|
Original Source
|
Check in Google Scholar
|
Full PDF (788.188 KB)
|
DOI: 10.21776/ub.jels.2019.009.01.06
<![CDATA[Porang tubers are plant commodities that physiologically susceptible to damage. Longtime storage can decrease the quality of tubers. Furthermore, the presence of fungi or bacteria makes tubers cannot be stored in a long time. Decreasing quality of Porang tuber became a problem because of the higher demand for porang tubers. This research was conducted to determine the suitable place and time storage of porang tubers. Therefore, farmers can control or reduce the decrease in tuber quality. Methods of this research were an observation of color change based on the Munsell Color System, odor observation, hardness observation using penetrometer, the rotten area of tuber was measured using Leaf Area Meter, shrinkage of tuber weight, the percentage of rotten and sprouted tubers, density, and moisture content by the oven method. The results of this study were analysed statistically by ANOVA test. The hardness of the tubers has a significant effect on the storage place (soil, rack, and floor) for period 14 weeks. Storage time has an effect on changes of the weight loss, moisture content and decay area. The longer the tuber stored the higher percentage of sprouted and rotten tubers. The color of porang tubers change and the odor in the part of the rotten tubers increase during the storage period in each storage place. Porang tubers should not be stored for more than two weeks and placed on a rack to minimize the damage of the tubers.Keywords: Physical change, porang tubers, storage time.]]>
