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Contact Name
Brigitta Laksmi Paramita
Contact Email
brigitta.laksmi@uajy.ac.id
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+6282329549978
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journal.biota@gmail.com
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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia
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Kota yogyakarta,
Daerah istimewa yogyakarta
INDONESIA
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Articles 18 Documents
Search results for , issue "Vol 14, No 1 (2009): February 2009" : 18 Documents clear
Akumulasi dan Toksisitas Effluen yang Mengandung Kromium pada Ikan Gupi (Poecilia reticulata) Djohan, Djohan
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.425 KB) | DOI: 10.24002/biota.v14i1.653

Abstract

Accumulation and toxicityofchromium in leather-tannery effluent on guppies were investigated. An acute (96 hour) statictoxicitytestingusing 5 serialdilution of theeffluentwas conducted. Each serial dilution consisted of 3 replicates, with eachreplicate contained 5litres of diluted effluent and 10 fish. Theeffluent was diluted 0.2%to 3.2%(v/v).Theconcentrations of chromium in thedilutedeffluent and fishrange from 0.9 - 9.1 µg.mL-1and 1.4 - 58.9 µg.g-1w.w, respectively. Bioconcentration factors inthisstudy vary from 1 to 39, with a median value of 6.2. The values of LC50and ILC50are 4.2 µg.mL-1and 56 µg.g-1w.w, respectively. The results show that chromiumaccumulates in guppy and causesacute toxicity. A comparison withthe concentrations of chromium in effluent, water, and fish from otherstudies was also reported.
Respon Pertumbuhan Bibit Picrasma javanica Blume terhadap Intensitas Naungan dan Media Tanam Setyowati, Ninik; Utami, Ning Wikan
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.011 KB) | DOI: 10.24002/biota.v14i1.2628

Abstract

Study on the growth response of Picrasma javanica Blume seedling to different shading intensities and medium was conducted at the Experimental Garden of Treub Laboratory, Research Centre for Biology, LIPI from June to December 2007. The research was arranged using Factorial in Completely Randomized Block Design with 2 factors and 5 replications. The first factor was shading intensities which were 0% (N0, without shading, average light intensities 39300 lux), 25% (N1, average light intensities 16430 lux), and 50% (N2, average light intensities 5867 lux), respectively and the second factor was medium (combinations of soil: manure:compost) with 6 levels were M1= 1:0:0, M2= 1:1:1, M3= 1:1:2, M4= 3:1:1, M5= 1:2:1, and M6= 2:1:1. The result showed that the N0 treatment (without shading) resulted the best growth response of Picrasma javanica Blume seedling, as showe in all parameters observed (plant height 27.11 cm; leaf number 15.57; diameter of trunk 4.32 mm; and root length of 15.97 cm, shoot dry weight of 1.762 g, root dry weight of 0.688 g and seedling quality index of 0.277). The growth media treatment of M5 (1-soil:2 manure:1 compost) showed the positive response on the growth of seedling better than other treatments and different with control (M1, soil media), with parameters were observed which was plant height 25.05 cm (M1= 19.10 cm); leaf number 16.53 (M1= 9.20); diameter of trunk 3.89 mm (M1= 2.76 mm); root lengh 15.23 cm (M1= 12.71 cm); shoot dry weight 1.58 g (M1= 0.663 g); root dry weight 0.51 g (M1= 0.221 g) and seedling quality index 0.220 (M1= 0.089). The combination treatment of N0 (without shading) and media M5 (1:2:1) gave the best response on the growth of Picrasma seedling (plant height 36.14 cm; leaf number 28.2; diameter of trunk 4.7 mm; and root length 16.7 cm, shoot dry weight 3 g and root dry weight 0.92 g).
Karakterisasi Genetik Kambing Gembrong dari Karangasem Bali Menggunakan DNA Mikrosatelit Puja, I Ketut; Sulabda, I Nyoman
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.575 KB) | DOI: 10.24002/biota.v14i1.2632

Abstract

The present study was undertaken with primary objective to characterize of Gembrong goat breeds. It is essential to characterize the germplasm for intragenetic variability, which will help in planning for conservation strategy as well as genetic improvement. DNA Genome was isolated from hairs. Nine microsatellite were amflified by PCR. PCR product were run on 6% bis-Acrylamide gel in automated DNA sequencer. Flourescent signals from the dye-labeled microsatellite were detecting using STRand software. The result show that all markers (INRA005, INRA063, INRA023, ILSTS19, ILSTS87, SRCRSP8, MAF65, OarFCB20, and McM547) successfully amplified in Gembrong goat microsatellite loci. The number of alleles per locus ranged from 1 (INRA005) to 4 (ILSTS87). All the microsatellites investigated were found to be highly polymorphic, except INRA063. In total, 23 alleles were observed for the 9 microsatellite loci. The allele sizes ranged from 99 bp (OarFCB20) to 240bp (SRCRSP8). The study can be extended to include large many microsatellites in different chromosome location to validate the results.
Aktivitas Antimutagen Isoflavon Glikosida Hasil Transglikosilasi Enzimatik CGT-ase Bacillus macerans Sulistyo, Joko; Soeka, Yati Sudaryati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.276 KB) | DOI: 10.24002/biota.v14i1.2629

Abstract

It has been known that isoflavone have biological activities such as antioxidant, antibacteria, antimutagenesis, and anticancer. Isoflavone aglycone uses such as genistein, daidzein and glycitein are limited since they are unstable and uneasily to dissolve in water. Through enzymatic transglycosylation reaction, its stability and solubility could be improved. In this study, genistin (isoflavone glycoside) was synthesized from genistein (isoflavone aglycone) by application of transfer reaction using enzyme cyclodextrin glucanotransferase (CGT-ase) of Bacillus macerans. Identification of the product was determined by TLC with methanol: chloroform (1:3, v:v) as eluent. Rf value 0.75 of the synthesized product was close to the Rf value standard of authentic genistin glycoside. The synthesized genistin was furthermore assayed to determine its antimutagenesis activity according to the Ames methode on E. minimal glucose media had been precultured with a mutant strain of Salmonella thypimurium TA98. The tested bacterial strain was induced with aflatoksin B1 as mutagen which had been activated with a lever homogenate. The result showed that the solubility and some biological properties of the synthesized genistin were improved higher than that of genistein, while it was found to be lower than that of the commercial genistin.
DAMPAK DERAAN KEKERINGAN P ADA FASE INISIASI BUNGA, FASE BERBUNGA DAN FASE PEMBENTUKAN POLONG T ERHADAP PEMBENTUKAN BUNGA DAN BUAH P ADA EMPAT VARIETAS VIGNA RADIATA Gaol, Mangadas Lumban; Ruma, Maria Longa; Sianturi, Hery Leo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.129 KB) | DOI: 10.24002/biota.v14i1.2634

Abstract

One of the greatest affected factors toward the growth of V. radiata is considered as the availability of water. Obviously, less production of V. radiata is due to short water supply. This research shows that flower initiation, flowering and fruiting to reproduction of four V. radiata varieties (Belu, Kenari, Perkutut and Murai), affected by drought are taken under this investigation. Based on numbers of initiated pod flowers and pods being produced, the most sensitive reproduction stages to drought in Belu variety were flower initiation and flowering, Kenari variety were flowering, Murai variety were flowering and fruiting, and Perkutut variety in all reproduction stages posses relatively similar level of sensitivity to drought. Generally, number of flowers initiated pods and the number of pods produced were higher in Kenari and Murai varieties compared to those on Perkutut and Belu. Based on numbers of flowers initiated pods and numbers of pods production, drought at flower initiation and flowering stage were more highly affected on Belu and Perkutut varieties compared to that of Kenari and Murai. Droughts on fruiting stage are highly affected on Perkutut varieties as well compared to other varieties. Therefore, based on plants production and their tolerance to drought, the best plants to be cultivated are Kenari and Murai.
Diatom dan Paleolimnologi: Studi Komparasi Perjalanan Sejarah Danau Lac Saint-Augustine Quebeq-City, Canada dan Danau Rawa Pening Indonesia Soeprobowati, Tri Retnaningsih; Hadisusanto, Suwarno
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.621 KB) | DOI: 10.24002/biota.v14i1.2635

Abstract

Diatoms are a micro-alga dominates in the aquatic ecosystem. Their silicious cell wall able to preserve death diatoms in the sediment for long periods of time, therefore, diatoms have an important role in the paleolimnological analysis. Diatoms assemblages in the sediment layer express the water quality whenever the diatom lives. This article provides information how to apply diatom on the paleo-limnological analysis, supporting with the case study in the Lac Saint-Augustine Quebec-City Canada and Rawa Pening Lake Indonesia. Modern diatom and the water quality from spatial and temporal range are used as a calibration set. The diatoms of below layers, then, Weighted Averaging (WA) with the calibration set to reconstruct the water quality in the past. Previously, both in Canada and Indonesia, those lakes were oligotrophic and sharply change into eutrophic condition since a lot of human activities developed around the lakes (anthropogenic factors). Naturally, the maturity of lake can not avoid and the succession had been fast by eutrophication. Paleolimnological approach provides baseline data in the past to develop the appropriate lake management.
Fitoekstraksi Sianida pada Centrosema pubescens Benth yang Tumbuh di Limbah Tailing Terkontaminasi Sianida Syarif, Fauzia; Hidayati, Nuril; Juhaeti, Titi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.918 KB) | DOI: 10.24002/biota.v14i1.2626

Abstract

Contamination of mined soil and water affects not only to agriculture system but alsofood chains and epidemiological problems. As soil metal can not be biodegraded,remediation of soil heavy metal risks has been a difficult and expensive goal. Presentlythere are several different strategies available for the clean up and restoration ofcontaminated soils. One approach to minimize risks from some toxic pollutants isphytoextraction using hyperaccumulator plants, known as phytoremediation. In thisresearch Centrosema pubescens was studied to examine its potency ashyperaccumulator against cyanide. Although cyanide is not categorized as heavymetal, its presence is considered as one of important toxic pollutants in theenvironments. Detoxification of cyanide (CN) contaminated soils and waters withplants seems to be a feasible option. Centrosema pubescens which proven tolerant anddominant in the CN contaminated environment was used in this research. The plantswere grown in tailing waste media added by 0, 10, 20 and 30 ppm CN. Different pHlevels were applied, i.e.+ 6 and +5. The results showed that the plants were capable ofgrowing under high level of CN. The results indicated that Centrosema pubescens canbe considered as high tolerance and potentially effective in accumulating CN, i.e up to3.564 ppm in roots and up to 3.564 ppm in shoot with concentration ratio (shoot/root)up to 1.13. The plants also indicated high tolerance by producing high biomass underhigh level of CN, i.e up to 36.517 g (under 20 ppm CN).
Understanding Food (Kajian Buku) Anugrahati, Nuri Arum
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i1.2636

Abstract

Kata understanding menarik untuk dipahami, terutama bagi pembaca yang tertarik dan memilih untuk menekuni jalur ilmu di bidang ilmu aplikatif, seperti teknologi pangan. Buku berjudul Understanding Food: Principles and Preparation yang menyajikan informasi mengenai ilmu pangan, gizi, dan food service, baik yang berupa prinsip-prinsip dasar maupun tren terbaru di bidang teknologi pangan menjadi pilihan yang menarik untuk dibaca.
Efek Kurkumin terhadap Pertumbuhan dan Perkembangan Fetus Rattus norvegicus L. Istriyati, Istriyati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i1.2631

Abstract

Curcumin which is isolated from Curcuma longa L. rhizome, has been used as a food additive, medicinal agent, cosmetic and fabric dye, whithout known its side effects. This research is to study the effects of curcumin on the structure of forelimb finger. Twenty virgin female rats, 2 month old, 150 g body weight were mated with fertile male rats. The present of vaginal plug or spermatozoa in the vagina were determined as the first day of pregnancy. Pregnant animals were devided into 4 groups of 5. Treatment were started from 10th to 17th day of pregnancy. The first 2 groups for control and vehicle only. Curcumin was dissolved in aquous 5% Tween 80. The second 2 groups were given curcumin at the dosage of 100 and 150 mg kg -1 bw. Rats were sacrificed at 18th day of pregnancy. Foetuses were dissected out, fixed in 10% formalin. Morphology of the foetuses were observed, forelimb were processed by paraffin method, and plantar paraffin section were made and used for histological observations and cell development of fingers. The results showed that curcumin at the dosage of 100 mg kg-1 body weight caused some foetuses dead, resorption of the embryo, hemorrhagi, unilateral phocomelia. Microscopical observations showed the treatment caused induced of nail formation, inhibition of indensity interdigity, and the increased of new blood vessel formation. It was concluded that the curcumin has no effect on the growth of foetuses. However it was affected the development of forelimb finger which is non dosage dependent.
Akumulasi dan Toksisitas Effluen yang Mengandung Kromium pada Ikan Gupi (Poecilia reticulata) Djohan Djohan
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i1.653

Abstract

Accumulation and toxicityofchromium in leather-tannery effluent on guppies were investigated. An acute (96 hour) statictoxicitytestingusing 5 serialdilution of theeffluentwas conducted. Each serial dilution consisted of 3 replicates, with eachreplicate contained 5litres of diluted effluent and 10 fish. Theeffluent was diluted 0.2%to 3.2%(v/v).Theconcentrations of chromium in thedilutedeffluent and fishrange from 0.9 - 9.1 µg.mL-1and 1.4 - 58.9 µg.g-1w.w, respectively. Bioconcentration factors inthisstudy vary from 1 to 39, with a median value of 6.2. The values of LC50and ILC50are 4.2 µg.mL-1and 56 µg.g-1w.w, respectively. The results show that chromiumaccumulates in guppy and causesacute toxicity. A comparison withthe concentrations of chromium in effluent, water, and fish from otherstudies was also reported.

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