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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

biota
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Published by Universitas Atma Jaya Yogyakarta

ISSN : 25273221 EISSN : 2527323X DOI : doi.org/10.24002/biota

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 50 Documents

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Search results for , issue "Vol 15, No 3 (2010): October 2010" : 50 Documents clear

Aktivitas Antibakteri Ekstrak Karang Lunak Sarcophyton sp. yang Difragmentasi dan Tidak Difragmentasi dari Perairan Pulau Pramuka, Kepulauan Seribu, Jakarta Kawaroe, Mujizat; Soedarma, Dedi; Effendi, Hefni; Nurhayati, Tati; Hardiningtyas, Safrina Dyah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Fragmented and non-fragmented soft corals showed antibacterial activities. Soft corals were gradually extracted using methanol, ethyl acetate, and hexane. Crude extract of the samples was tested its antibacterial activity, Minimun Inhibitory Concentration, toxicity (Brine Shrimp Lethality Test method), and phytochemicals. Overall, the antibacterial activity of crude extract of non-fragmented soft coral Sarcophyton sp. was higher than the crude extract of fragmented soft coral Sarcophyton sp. Crude ethyl acetate extract showed higher antibacterial activities. The ethyl acetate crude extract of non-fragmented soft coral Sarcophyton sp. is able to inhibit all tested bacteria is E. coli, S. aureus, P. aeruginosa and B.cereus, while the ethyl acetate crude extract of fragmented Sarcophyton sp. is unable to inhibit bacteria P. aeruginosa. Minimum inhibitory concentration extracts of non-fragmented Sarcophyton sp. in range 240âˆ’480 Î¼g/disc. The 24-h LC50 extracts of fragmented and non-fragmented Sarcophyton sp. for Artemia salina were 149.50 ppm and 45.15 ppm, respectively. Bioactive compounds of fragmented and non-fragmented Sarcophyton sp. extract are steroid, flavonoid and alkaloid.

Pengaruh Ekstrak Daun Nerium oleander L. terhadap Mortalitas dan Perkembangan Hama Spodoptera litura Fab. Prabowo, Heri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Research was conducted at the Laboratory of Entomology, Tobacco and Fiber Crops Research Center from June to July 2009. Tests were conducted on the larvae of Spodoptera litura second instar. Concentration of leaf extract of N. oleander used was 0; 2x105; 3x105; 4x105; and 5x105ppm. Treatment was done by spraying method in the spray chamber. Treatment was arranged in a completely randomized design (RAL) with 4-time repetition. Each repetition used 25 larvae. Observed parameters included the death for every 24, 48, 72, and 96 hours; length, width, and weight of larvae after 96 hours after treatment. By 96 hours after treatment with the concentration of leaf extract 0; 2x105; 3x105; 4x105; and 5x105ppm it was capable of causing mortality S. litura, respectively for 0; 48.33; 53.33; 66.67%, dan 76.67%. By 96 hours after treatment extract concentration required to cause mortality S. litura was 25, 50, and 95% respectively of 2.35x105, 4.44x105, and 9.49x105ppm. After 96-hour treatment the concentration of leaf extract 0; 2x105; 3x105; 4x105; and 5x105ppm could cause the length, width, and weight of S. litura, respectively for 0.89 mm; 0.91 mm; 0.93 mm; 0.94 mm; 0.95 mm; 0.15 mm; 0.16 mm; 0.17 mm; 0.18 mm; 0.19 mm, 0.011 g; 0.012 gr; 0.013 g; 0.014 g and 0.016 gr. The use of N. oleander leaf extract caused mortality of S. litura between 13.33âˆ’76.67% at 24 to 96 hours after treatment. Leaf extract of N. oleander potentially caused mortality S. litura. The use of this leaf extract could also inhibit the length, width, and weight of larvae.

Kandungan Fenol dan Aktivitas Antioksidan Makroalga Bentik Caulerpa racemosa (Forsskal) dari Teluk Hurun, Lampung Santoso, Joko; Fitriani, Diini; Wardiatno, Yusli
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Caulerpa racemosa is a green benthic macroalga that mainly grows in tropical regions which is expected to bask of strong ultraviolet radiation from sunlight. This circumstance can cause to increase levels of reactive radical species. To reduce and/or protect, organism like macroalga may change its metabolism and stimulate to produce some active compounds, therefore, tropical macroalgae are estimated possessing a large number of active compounds such as antioxidant. In this experiment, edible green benthic macroalga Caulerpa racemosa grown in different water condition were used. The content of total phenol and antioxidant activity of ethyl acetate extract were performed. Pearson correlation between waters condition and antioxidant activities i.e. total phenol and DPPH inhibition were also analyzed. Caulerpa racemosa grown in station 2, exposed in strong radiation from sunlight, had the highest content of total phenol and percentage of DPPH inhibition, with their values were 12.60% and 46.43% respectively. Sunlight intencity in waters had strong positive correlation to the total phenol content and reducing activity of DPPH, however, parameters of nitrate and ammonia had strong negative correlation.

Potensi Mangrove dan Manfaatnya bagi Kelompok Etnik di Papua Arobaya, Agustina; Pattiselanno, Freddy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

significant contribution to those who living in and around mangrove forests. Like any othercommunities who occupied areas surrounding forest sites, Papuan ethnic groups really connectand integrate with the forest environment such as mangrove forests. This paper is a review ofrelated literatures that compiled from different research which have been conducted inUniversitas Negeri Papua (UNIPA) on utilization of mangrove by local ethnic groups and its rolefor other life biota in Papua. The results indicated that mangrove plays a very important rolefor local tribes in Papua as food sources, construction materials and source of energy,traditional medicine sources and wildlife habitat that are usually consumed by the communities.Ecology-based tourism or ecotourism as one leading sector of mangrove forests in Papua,though has a good potential, has not been optimizedly used.

Optimasi Produksi Poli-Î²-Hidroksibutirat (PHB) oleh Bacillus sp. PSA10 Yanti, Nur Arfa; Margino, Sebastian; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

A new strain characterized as Bacillus sp. PSA10 was found to produce poly-Î²-hydroxybutyrate (PHB) at concentration of 52.28% (g PHB/g dry cell weight) in shaken flask culture, using sago starch as a carbon source. This research is aimed to determine the optimum culture condition of PHB production Bacillus sp. PSA10 at laboratory scale. Optimization of PHB production was conducted in this research, in terms of inoculum concentration, concentration of the major components in minimal medium, environmental condition and incubation time. The result showed that optimum conditions for the production of PHB by Bacillus sp. PSA10 were achieved at minimal medium (Ramsay medium) with 5% (v/v) inoculum concentration, 2% (w/v) sago starch, 1.0 g/l (NH4)2SO4, 6.7 g/l Na2HPO4.7H2O, and 0 g/l KCl. The optimum environmental conditions were achieved with initial pH 7, temperature 37oC, agitation speed at 150 rotary per minute (rpm) and the best of incubation time was 48 hour. Under this optimum condition, the maximum PHB production by Bacillus sp. PSA10 increased from 52.28% to 71.35% (g PHB/g dry cell weight) at 48 hour cultivation. Therefore, Bacillus sp. PSA10 is potential to apply for PHB production from sago starch at industrial scale.

Pengaruh Pupuk Nitrogen dan Ethephon terhadap Pertumbuhan, Pembungaan dan Hasil Padi Lokal (Oryza sativa L. cv. Rojolele) Rachmawati, Diah; Maryani, Maryani; Setyaningsih, Tri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Physiological studies on Rojolele rice were conducted after the application of nitrogen fertilizer and ethephon at different doses. Application of nitrogen fertilizer is one of the most important measurements to improve plant growth and yield of rice. Ethylene which has flowering initiate roles are induced by ethephon. The objective of this study was to analyze the effect of nitrogen fertilizer and ethephon on growth, flowering and yield of rice (Oryza sativa L. cv. Rojolele). The experiment was carried out using Complete Randomized Design with 2 factors, which each factor consisted of four levels. The first factor was doses of urea as nitrogen source 0, 50, 100 and 150 kg ha-1. The second factor was doses of ethephon 0; 100; 200 and 400 mg l-1. Measured parameters were plant height, tiller number, plant biomass, root shoot ratio, flowering time, yield and grain size. The application of nitrogen fertilizer improved the plant growth of Rojolele rice. Plant height, tiller number, plant biomass, panicle length, and number of grains increased by the increasing doses of nitrogen, but this factor did not significantly affect on grain size. Ethephon application reduced plant height and delayed flowering time. However, it simultaneously increased tillers when compared at the same N dose. Interaction between nitrogen fertilizer and ethephon gave significantly effects on the plant height and number of grains per panicle, but there were no significant effect on the rice grain size.

Enkapsulasi dan Regenerasi Kalus Embriogenik Mangga (Mangifera indica L.) Kultivar Bapang dan Gadung 21 Ermayanti, Tri Muji; Nugroho, Robertus; Hamim, Hamim
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

The mother plant and genetic variability of Indonesian mango need to be conserved. Encapsulation is one of in vitro conservation used for many plant species. The aim of this research was to study the regeneration of encapsulated mango cultivar Bapang and Gadung 21 embryogenic callus after storage at -14, 3âˆ’5, and 26âˆ’27oC for 0, 1, 2, 4, and 8 weeks. Embryogenenic callus was treated with 3% Na-alginate (in liquid 3M), then it was dropped into 100 mM CaCl2. Encapsulated callus beads were dehidrated and stored at -14, 3âˆ’5, and 26âˆ’27oC for 0, 1, 2, 4, and 8 weeks. After storage, the callus was cultured in 3M medium containing 2,4-D at 0, 1, and 2 mg/l for the regeneration. The results showed that after 8-week storage, callus of Bapang culticar was more viable (67.3%) and resulted more number of somatic embryos (191.6%) than Gadung 21 cultivar. The callus which was cultured in 3M medium without addition of 2,4-D was more viable (20.9%) and had more number of somatic embryos (1062.5%) than that which was cultured on medium containing 2,4-D. After 2-week storage, callus had viability of 7.6%. No storage callus formed more somatic embryos than storage callus. Storage at 26âˆ’27oC gave higher viability as well as higher number of somatic embryo than stored at -14 and 3âˆ’5 oC. The callus did not regenerate into shoots after storage at -14oC. Embryogenic callus could be stored at 3âˆ’5 and 26âˆ’27oC for 4 weeks.

Purifikasi Kolagenase dari Usus Bandeng (Channos channos, Forskal) Yuniarti, Tatty; Nurhayati, Tati; Jacoeb, Agoes Mardiono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Collagenase was purified from intestines of milkfish (Chanos chanos, Forskal) by extraction,ammonium precipitation, ion exchange chromatography on DEAE Sephadex A-50 and gelfiltration on a Sephadex G-100 column. The purification and yields were 114.731 fold and1.26% when compared to those in the starting-crude extract. The molecular mass of milkfishserine collagenase was estimated to be 14.63 kDa and 27.46 kDa.

Aktivitas Antibakteri Bacillus yang Berasosiasi dengan Landak Laut di Pantai Mentigi, Lombok Barat Suryadi, Bambang Fajar; Febrian, Novi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

The search for new antimicrobial agents is very significant. The prevalence of antimicrobialresistance among key microbial pathogens is increasing at an alarming rate worldwide. Bacillusspecies produce many kinds of antibiotics which share a full range of antimicrobial activities.The aim of this research is to study antibacterial activity of three bacillus isolates (1A, 2J, 3L)which were isolated in Mentigi Beach, West Lombok. Assessment for antibacterial activity wasconducted using Overlaid Molten agar method on Nutrient Agar medium at different salinity(concentration of NaCl 0, 5, 10%) and different pH (pH 6, 7 and 8). The study showed that therewere different antibacterial activities at different salinity and different pH media. Production ofantibacterial at 0% NaCl concentration occurred only at pH 6 and 7 and had narrow spectrumcharacter (only for Gram positive bacteria). Addition of 5% NaCl made antibacterialproduction might occur at pH 6, 7 and 8 and had wide spectrum character (for Gram positiveand negative bacteria).

Variasi Genetik pada Rusa Sambar (Rusa unicolor) di Penangkaran, Kabupaten Penajam, Kalimantan Timur Wirdateti, Wirdateti; Brahmantyo, Bram; Semiadi, Gono; Reksodihardjo, Andi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Sambar deer (Rusa unicolor) is the largest Indonesian deer species. The largest population of captivation is located at Penajam district, East Borneo (UPTD). First population was introduced in 1990 with four individuals. The use of molecular marker was aimed to identify and characterise the level of genetic diversity within the UPTD population as well as to identify a possible of botolneck population genetic status. This study discussed the relevance of the result for management purposes of captivation. The results indicated that sambar deer populatin (n=38) had an average genetic distance (d) in population as 0.006 with nucleotide diversity (Ï€) being 0.0159). A total of 43.48% of the population was homogeneous that showed no nucleotide differences among individuals.

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Brigitta Laksmi Paramita

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journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

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Abstract

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Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Brigitta Laksmi Paramita

Contact Email brigitta.laksmi@uajy.ac.id

Phone +6282329549978

Journal Mail Official journal.biota@gmail.com

Editorial Address Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

Phone
+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA