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Brigitta Laksmi Paramita
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,193 Documents
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The Life Cycle and Sensitivity of the Local Copepod, Apocyclops sp to Tributyltin Exposure Inneke F.M. Rumengan; N.D. Rumampuk; D. Sumilat; J. Rimper
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i2.2690

Abstract

Uji toksisitas tributiltin secara akut telah dicobakan pada kopepoda tropis Apocyclopssp. yang diisolasi dari tambak Manembo-nembo Bitung, Sulawesi Utara. Kopepodadikultur dalam kondisi laboratorium (25-27oC, 30 ppt dan tanpa penerangan) denganpemberian mikroalga Nannochloropsis oculata sebagai pakan. Semua individukopepoda yang digunakan sebagai hewan uji berasal dari sepasang induk jantan danbetina. Kopepoda untuk eksperimen tributiltin (TBT) diberi perlakuan dalam air lautdan selama eksperimen tidak diberi pakan, dan larutan stok TBT-Cl dilarutkandalam aseton. Pengaruh starvasi (tanpa pemberian pakan) dan aseton diamatisebelum uji toksisitas TBT dilakukan. Setiap eksperimen, 10 kopepoda dewasa (5jantan dan 5 betina) dari satu kohort dimasukkan ke dalam cawan petri (diameter 3cm) berisi masing-masing 10 ml air laut. Ternyata perlakuan tanpa pemberian pakantidak mempengaruhi kopepoda selama periode eksperimen. Dalam uji toksisitas TBT,hanya 3 individu yang dapat bertahan sampai akhir eksperimen (8 jam) walaupundengan konsentrasi terendah (0.0001 ng.l-1). Kebanyakan individu telah mati sebelum8 jam diekspos ke konsentrasi TBT 0.01 ng.l-1. Pada konsentrasi TBT yang lebihtinggi (0.1 dan 1 ng.l-1), tingkat kelulusan hidup kopepoda hanya 50% dalam waktukurang dari satu jam, sedangkan kopepoda yang sisa masih hidup semuanya sebelummati jam ke-4 yang diberi perlakukan. Dalam uji toksisitas ini, semua konsentrasiyang dicobakan ternyata lebih kecil dari rata-rata konsentrasi TBT di alam (10 ng.l-1).Kisaran konsentrasi TBT yang lebih lebar masih perlu diuji-cobakan untukmengklarifikasi efek akut TBT agar dapat diperoleh konsentrasi untuk uji toksisitassecara kronis.
Molecular Identification of Genes Involved in Magnetosome Synthesis in Magnetospirillum magneticum AMB-1 Aris Tri Wahyudi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i2.2691

Abstract

Satu mutan Magnetospirillum magneticum AMB-1 yang tidak bersifat magnetik, yang didesain NMA41, dikonstruksi melalui mutagenesis dengan transposon Mini-Tn5Km1 untuk mengidentifikasi gen yang terlibat dalam sintesis magnetosom. Mutagenesis dengan transposon dilakukan melalui konjugasi antara M. magneticum AMB-1 dan Escherichia coli S17-1 ( pir) yang membawa plasmid pUTmimi-Tn5Km1. Frekuensi transkonjugasi tertinggi berkisar 1.8 x 10-7 sel per resipien. NMA41 tidak respon terhadap bidang magnet dan kehilangan kemampuan dalam mensintesis magnetosom. Sekuens DNA/gen yang disisipi oleh transposon (dinamakan DNA pengapit) diisolasi dengan PCR yang dibalik (inverse PCR) dan diklon ke dalam plasmid pCR2.1. Penyejajaran sekuen DNA dari DNA pengapit terhadap sekuens DNA genom lengkap AMB-1 dapat mengidentifikasi sebuah kerangka baca terbuka (open reading frame, ORF2) dalam suatu operon yang terdiri dari 4 gen. Sekuen asam amino yang dideduksi dari ORF2 menunjukkan homologinya dengan protein domain GGDEF dari Magnetospirillum magnetotacticum MS-1 (identik 90%; kemiripan 95%) yang mempunyai fungsi dalam mekanisme transduksi sinyal. Gen atau operon ini diduga berfungsi selama proses sintesis magnetosom pada M. magneticum AMB-1.
Era Emas Biologi P. Kianto Atmodjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i2.2692

Abstract

Selamat datang dalam era emas biologi! Setiap hari berita-berita yang berkaitan dengan biologi selalu bermunculan melalui koran harian, mingguan, dan berita-berita yang disiarkan media elektronika. Pemanfaatan biologi untuk bidang industri manufaktur, pertanian, kesehatan, farmasi dan makanan tidak pernah ada habisnya. Hal yang paling hangat adalah pemanfaat teknologi asam deoksi ribonukleat untuk penentuan jati diri teroris yang terbunuh dalam penyergapan di Temanggung apakah Nordin M Top atau bukan, penentuan jati diri korban ledakan bom atau kebakaran, serta penentuan jati diri korban kecelakaan pesawat, merupakan contoh-contoh peran biologi dalam kehidupan bermasyarakat modern, dan inilah yang dimaksud era emas biologi oleh Reece dan Simon dalam pengantar buku Essential Biology With Physiology.
Analisis Filogenetik Burung Maleo (Macrocephalon maleo) Berdasarkan Sekuen Intron Satu Gen Rhodopsin (RDP1) Nukleus I Made Budiarsa; I Wayan Tunas Artama; Langkah Sembiring; Jesmandt Situmorang
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2693

Abstract

The phylogenetic relationships of the maleo (Macrocephalon maleo) were analyzed based on thefirst intron of rhodopsin nuclear gene sequence data obtained from 15 individuals, along withthose of 22 individuals taken from GenBank. The phylogenetic trees were reconstructed byNeighbor-Joining (NJ) method. Results indicated that 956 bp of RDP1 sequence, 414 (43.4%)sites were variable and 317 (33.2%) sites were phylogenetically-informative. The basecomposition for all species analyzed in this research were as follows: T 25.3%, C 26.3%, A18.5%, and G 29.9%. Analysis of RDP1 sequence produced trees that were remarkably wellresolved and had topologies at the marga level. The phylogenetic analysis showed that maleowas monophyly of Macrocephalon and closely related to Aepypodius, Talegalla, Leipoa andAlectura.
Jenis Flora Asing Invasif di Taman Nasional Gunung Gede Pangrango, Jawa Barat Tahan Uji; Sunaryo Sunaryo,; Erlin Rachman; Eka Fatmawati Tihurua
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2694

Abstract

Ecological study of Invasive Alien Species (IAS) at two altitudes in Mount Gede PangrangoNational Park was carried out by using quadrate method. The studied plots located at altitude1400 m and 1500 m a.s.l, in 0.2 ha each. The result shows that 45 species of trees, 77 species ofsaplings and 48 species of seedlings were recorded. Five species (i.e. Bartlettina sordida,Austroeupatorium inulaefolium, Cestrum aurantiacum, Brugmansia suaveolens and Passiflorasuberosa) are catagorized as IAS which threaten the ecosystem and natural species.
Struktur dan Komposisi Jenis Tegakan Semai dan Pancang di Hutan Alam Akibat Pemanenan Kayu dengan Teknik Reduced Impact Timber Harvesting Diana Sofia Hanafiah; Muhdi Muhdi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2695

Abstract

The effect of reduced impact timber harvesting technique to structure and species composition of seedling and poles was studied using the data of three plots each sized 100 m x 100 m, which are placed randomly at landing, middle skiddtrail and tips of skiddtrail, respectively. The aim of the study was to know the effect of reduced impact timber harvesting technique to structure and species composition of seedling and poles in tropical forest. The research showed that the forest harvesting caused the changing of the structure and species composition of seedling and poles in natural tropical forest.
Kandungan Protein dan Isoflavon pada Kedelai dan Kecambah Kedelai Hery Winarsi; Agus Purwanto; Hidayah Dwiyanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2696

Abstract

The research was conducted to explore protein and isoflavone content on soy non germed and soy germed. Soybean “slamet” was rinsed, dipped over night, drained, put in a moist room, and water sprinkled once in a while until germed. The husk of soy and soy germ were removed, and then the soy and soy germ were extracted by NaCl physiologic to become slurry. The slurry pH was arranged to 5.0, centrifugated at 10.000 rpm, and the precipitation was dryed in 70oC to obtain the soy protein and the soy germ protein flour. The levels of protein and isoflavone flour were determined. The result showed that protein content of soy was 36.5%, while protein of soy germ was 42%. The isoflavone of soy protein was 26.7 ppm, consisting of 11.5 ppm genistein, 10.2 ppm daidzein and 5 ppm glicitein, but the soy germ protein was 39.1 ppm, consisting of 14.6 ppm genistein, 16.9 ppm daidzein and 7.6 ppm glicitein. The conclusion is that germed process could increase protein and isoflavone content. The soy protein isoflavone was dominated by genistein and daidzein, while soy germ protein isoflavone was dominated by daidzein and glicitein.
Perilaku Harian Buaya Muara (Crocodylus porosus, Schneider 1801) di Pusat Penyelamatan Satwa Jogja Purwo Setio I.; A. Fanani Muharromi; Subekti Prihantono; Tony Febri Qurniawan; A. Prima Nugraha; Rury Eprilurahman
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2697

Abstract

The aim of this research was to find out the daily behaviour of Crocodylus porosus in Jogja Animal Rescue Center (PPSJ). The daily behaviour included feeding habit, social behaviour, basking and movement. This research was using Focal Animal Sampling Method and observed for four months. As individual targets were male and female dominant of C. porosus, Monti and Bunda. The Runs test showed that the behavior of C. porosus had a pattern in daily activities. Based on Mann-Whitney U test, both male and female of C. porosus did not have any difference in feeding habit (U=16; n1= 6; n2=6; p>0,05), social behaviour (U=29; n1= 8; n2=8; p>0,05), basking (U=12; n1= 5; n2=5; p>0,05) and movement (U=16; n1= 6; n2=6; p>0,05).
Biotransformasi Pirokatekol Glikosida Menggunakan Kultur Suspensi Sel Solanum mammosum L. Yati Sudaryati Soeka; Joko Sulistyo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2704

Abstract

A syntesis of pyrocathecol glucoside was carried out by applying biotransformation cell culture suspension from calus of Solanum mammosum L., on modified medium of Murashige and Skoog (MS). A growth maximum volume at 15,5 ml of cell culture suspension of S. mommosum was achieved on day-8 incubation. The results showed that pyrocathecol glucoside as a bitransformation product that was obtained by application of Pyrocathecol at 50-200 ppm was determined by TLC and identified at Rf value of 0.82−0.83. Futhermore, the biotransformation products were determined by HPLC obtained from the cell culture suspension at concentration of 200 ppm pyrocathecol so that resulted in reaction products based on standard solution. The peaks number 1, 2 and 3 with retention time 2.53 min, 4.62 min and 7.58 min were appropriate to the retention time cellobiose, glucose and methyl α-glucoside, respectively. Peak number 4 with retention time 8.52 min conformed to pyrocathecol-glucobioside as a product of side transfer and peak number 5 with retention time 10.52 min in line with the retention time of arbutin were pyrocathecol-glucoside as a transfer product expected from the result of biotransformation.
Kelimpahan Cacing Tanah pada Beberapa Jenis Tegakan Pohon di Wanagama I Haryono Supriyo; Musyafa Musyafa; Arom Figyantika; Saptuti Gamayanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2705

Abstract

Earthworms feed exclusively on dead organic matter (OM). The earthworm population is regulated by organic matter availability and soil characteristics. The aim of the research was to investigate the effect of seasonal change to the abundance and biomass of earthworms in Wanagama I. Collection of the soil samples was conducted from a quadrangle 25 x 25x 25 cm with the depth of 0-10, 10-20 and 20-30 cm with 5 replications under stands of Glyricidae, Leucaena, Teak, Acacia, Eucalyptus and Mahogany. Soil samples were taken at rainy season (February), early dry season (July) and dry season (October) 2004. The earthworms were manually extracted from soil samples and the abundance was stated as number and biomass of dry weight. The result showed the abundance and biomass of earthworms were high only in February at the soil depth of 0-10 cm. The highest number of earthworm and biomass was found in Mahogany stand (224,000 individual.ha-1/105.6 kg.ha-1). Multiple linier regression analysis showed that abundance of earthworms was affected by soil moisture content, while the biomass of earthworms was affected by soil pH and bulk density (aeration).

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