cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Brigitta Laksmi Paramita
Contact Email
brigitta.laksmi@uajy.ac.id
Phone
+6282329549978
Journal Mail Official
journal.biota@gmail.com
Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia
Location
Kota yogyakarta,
Daerah istimewa yogyakarta
INDONESIA
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,224 Documents
 119   120   121   122   123 
Pengaruh Lama Pelayuan dan Penyulingan Terhadap Rendemen serta Karakteristik Minyak Serai Wangi (Cymbopogon nardus L.) Rina Sardillah Mae; MM Endah Mulat Satmalawati; Jefrianus Nino
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2026): June 2026
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.14707

Abstract

(Cymbopogon nardus L.) is an important commodity with high economic value, but its quality and yield are greatly influenced by post-harvest processes. This study aims to determine the effect of withering and distillation on the yield, quality, and characteristics of citronella oil. The study was conducted using a Completely Randomized Design (CRD) with two variables, namely withering (1, 2, and 3 days) and distillation (2, 4, and 6 hours). The parameters observed were oil content, specific gravity, refractive index, solubility in 80% ethanol, and sensory assessments related to color and aroma. The duration of withering and distillation time had a significant effect on the quantity of citronella oil. The best oil yield of 0.8% was obtained from a three-day withering process with a six-hour distillation time, while the lowest yield of 0.5% was obtained from a one-day withering process and a two-hour distillation.  The resulting parameter values include specific gravity (0.893–0.899 g/mL), refractive index (1.467–1.470), and solubility (1:2), all of which meet the standard requirements set by SNI 06-3953-1995. Sensori- cally, the oil is pale yellow to brownish in color with a distinctive lemongrass aroma and is acceptable to the panelists.  
Effectiveness of Crescentia cujete Linnaeus Fruit Ethanol Extract as Aedes aegypti Linnaeus Larvicidal Veni Hoeriah; Sofia Ery Rahayu; Rahmi Masita
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2026): June 2026
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.9780

Abstract

Dengue fever vector control currently uses chemical larvicides, but the use of chemical larvicides is considered less effective because Ae. aegypti larvae have developed resistance. Berenuk plant (C. cujete) is one of the plants that has potential as a larvicide. The aim of the research was to analyze the effectiveness of C. cujete fruit skin extract and flesh extract as an Aedes aegypti larvicide and to determine the content of active compounds found in the skin and flesh of berenuk fruit. Phytochemical screening of fruit skin extract and fruit flesh extract using thin layer chromatography test. Mortality analysis of Ae. aegypti larvae using Two-way Anova and probit to determine the LC50 value. Research results show that C. cujete fruit skin and flesh extract contained alkaloid, flavonoid and triterpenoid compounds. C. cujete fruit skin extract and flesh extract can cause mortality of Ae. aegypti larvae with LC50 values, namely the concentration of fruit skin extract 3415.007 ppm and fruit pulp extract 3112.767 ppm during 96 hours of observation. Fruit skin extract and fruit flesh extract of C. cujete have larvicidal effect against Ae. aegypti.
In Silico Study of Black Garlic (Allium sativum) Against COX-2 in Anti-Inflammatory Therapy Riki Agustin Maulana; Alya Azzahra Ramadhani; Nafisa Shoya Ifara; Dzava Prawinsyah Fairus Ismail; Febby Pratama; Sabiq Salmandhiya Harits; Rina Fajri Nuwarda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2026): June 2026
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i2.14292

Abstract

Cyclooxygenase-2 (COX-2) is an inducible enzyme involved in prostaglandin biosynthesis during inflammatory processes and represents an important therapeutic target for anti-inflammatory drug development. Black garlic (Allium sativum) contains bioactive compounds such as S-allyl cysteine (SAC), 5-hydroxymethylfurfural, and flavonols with reported anti-inflammatory potential. This study aimed to investigate the potential of black garlic-derived compounds as COX-2 inhibitors using an in silico approach. Twenty-four compounds were evaluated through Lipinski’s Rule of Five, ADMET prediction, ligand-based pharmacophore screening, and molecular docking using the COX-2 crystal structure (PDB ID: 3LN1), with celecoxib as the reference inhibitor. In contrast, 5-hydroxymethylfurfural demonstrated high intestinal absorption and strong pharmacophore similarity to celecoxib, although its binding affinity was comparatively low. Flavonols showed the best docking performance, characterized by binding energy of −8.18 kcal/mol, high pharmacophore fit scores, and acceptable pharmacokinetic profiles. However, toxicity prediction suggested that both 5-hydroxymethylfurfural and flavonols may possess mutagenic and carcinogenic potential. Overall, flavonols and 5-hydroxymethylfurfural may warrant further investigation as potential COX-2-targeting compounds, although molecular dynamics, in vitro, and in vivo studies are needed to validate their activity and safety.
Pengaruh Variasi Komposisi Bioaktivator pada Kompos Pelepah Pisang (Musa acuminata L.) terhadap Diversitas Mesofauna Tanah Alifyanita Maulida 'Ainur Rahma; RR. Upiek Ngesti Wibawaning Astuti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2026): October 2026
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.9176

Abstract

Pisang (Musa acuminata L.) merupakan salah satu komoditas pertanian dengan permintaan pasar yang terus meningkat di masyarakat. Akan tetapi, limbah hasil pertanian pisang seperti pelepah belum banyak dimanfaatkan dan diolah secara optimal. Pembuatan kompos dapat menjadi alternatif pengolahan limbah pelepah pisang, karena mengandung senyawa potensial yang bermanfaat. Keberadaan mesofauna tanah merupakan salah satu bioindikator yang dapat digunakan untuk menilai kualitas kompos. Penelitian ini bertujuan untuk menjelaskan diversitas, pengaruh variasi komposisi bioaktivator, dan analisa korelasi parameter lingkungan terhadap mesofauna tanah, terutama keberadaan Acarina dan Collembola pada kompos berbahan dasar pelepah pisang. Variasi komposisi bioaktivator yang digunakan adalah perbandingan probiotik : molase, yaitu 3:3 (P0), 9:3 (P1), 3:9 (P2), dan 9:9 (P3) dengan 3 kali ulangan. Parameter lingkungan yang diukur berupa suhu, pH, dan kelembapan kompos. Analisis data dilakukan dengan menggunakan indeks keanekaragaman Shannon-Wiener. Hasil penelitian ini menunjukkan bahwa ditemukan 11 famili Acarina (Acaridae, Bdellidae, Cunaxidae, Ascidae, Blattisociidae, Dermanyssidae, Laelapidae, Macrochelidae, Macronyssidae, Parasitidae, dan Uropodidae) dan 3 famili Collembola (Entomobryidae, Isotomidae, dan Tomoceridae), dan diversitas mesofauna dikategorikan dalam kategori sedang. Variasi komposisi bioaktivator, parameter lingkungan seperti suhu, kelembapan, berpengaruh positif, sedangkan pH kompos berpengaruh negatif terhadap keberadaan mesofauna tanah, khususnya Acarina dan Collembola.
Isolasi dan Evaluasi Aktivitas Antifungi Jamur Endofit Tanaman Pisang Kepok Terhadap Fusarium oxysporum Oktira Roka Aji; Aninda Kumala Sari
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2026): October 2026
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.12246

Abstract

Pisang (Musa spp.) merupakan komoditas hortikultura penting yang produktivitasnya dapat terganggu oleh penyakit layu Fusarium yang disebabkan oleh Fusarium oxysporum f. sp. cubense (Foc). Jamur endofit berpotensi dikembangkan sebagai agens hayati karena dapat berinteraksi secara antagonistik dengan patogen, antara lain melalui kompetisi dan produksi senyawa volatil. Penelitian ini bertujuan mengisolasi dan mengevaluasi aktivitas antifungi jamur endofit dari pisang Kepok (Musa × paradisiaca L.) terhadap Foc. Jamur endofit diisolasi dari akar, batang, dan daun tanaman sehat, kemudian dikarakterisasi berdasarkan ciri makroskopis dan mikroskopis. Aktivitas antagonistik dievaluasi menggunakan metode kultur ganda, sedangkan aktivitas penghambatan oleh senyawa volatil diuji menggunakan metode cawan petri bertangkup. Sebanyak enam isolat diperoleh dan berdasarkan karakteristik morfologinya diduga termasuk dalam genus Fusarium, Trichoderma, Mortierella, Trichothecium, Rhizoctonia, dan Pythium. Pada uji kultur ganda, isolat B1 yang diduga sebagai Trichoderma menunjukkan dominasi pertumbuhan terhadap Foc yang mengindikasikan kompetisi ruang dan nutrisi. Pada uji senyawa volatil, B1 menghasilkan persentase penghambatan tertinggi sebesar 30,74 ± 1,74%. Hasil penelitian menunjukkan bahwa isolat B1 memiliki potensi untuk dikembangkan lebih lanjut sebagai kandidat agens hayati terhadap Foc, meskipun identifikasi molekuler dan pengujian lanjutan masih diperlukan.
Galangal Rhizomes Phytoconstituents as VEGFR2 Inhibitors in Breast Cancer: Computational studies Keisya Ghaliyah Purnomo; Gabriel Daendya Amalia Bintang Christy; Jahraema Deagustia Ningsih; Jihan Sadira Aurellia; Dhania Novitasari
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2026): October 2026
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.12790

Abstract

Breast cancer is the most frequently diagnosed cancer and the leading cause of cancer death among women in Indonesia. Vascular endothelial growth factor receptor 2 (VEGFR-2), a key regulator of tumour angiogenesis, is an established therapeutic target, but existing inhibitors are costly and poorly tolerated. This study evaluated ten compounds reported from Kaempferia galanga rhizomes as potential VEGFR-2 inhibitors using in silico approaches, including Lipinski’s Rule of Five, ADMET prediction with PreADMET, pharmacophore screening with LigandScout, and molecular docking against VEGFR-2 (PDB ID: 3VO3). All ten compounds satisfied the Rule of Five. The selected pharmacophore model (Model 4, AUC = 0.78) retrieved eight of them as hits. Redocking of the native imidazo[1,2-b]pyridazine ligand gave an RMSD of 1.29 Å, a binding energy of -10.81 kcal/mol and a Ki of 11.93 nM, validating the protocol. Sandaracopimaradien-6β,9α-diol-1-one gave the lowest binding energy among the test compounds (-9.23 kcal/mol; Ki 172.53 nM) but formed no hydrogen bonds and failed the pharmacophore filter. Seven phenolic compounds reproduced the native ligand’s hydrogen bond to Glu885. Taking drug-likeness, predicted toxicity, pharmacophore fit and binding mode together, methyl (2R,3S)-2,3-dihydroxy-3-(4-methoxyphenyl)propanoate was the most promising VEGFR-2 inhibitor candidate, and confirmation by in vitro and in vivo testing is required.
DNA Isolation and Purification Protocols for DNA Amplification Enhancement in Monochoria vaginalis PCR Analysis Kansa Dianti Putri; Ramadaniarto Rizqullah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2026): October 2026
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.12875

Abstract

Monochoria vaginalis contains phenolic chemicals that can disrupt DNA extraction and downstream molecular processes. Phenolics are known to bind to DNA and hinder enzymatic processes, which affects procedures like PCR.  The goal of this study was to create an effective DNA extraction and purification method for obtaining high-quality genomic DNA from M. vaginalis. The DNA was extracted using a modified CTAB technique and purified with 30 μL sodium acetate and 1,000 μL 70% ethanol. The purification procedure enhanced DNA quality, as seen by less smearing and cleaner bands in electrophoresis, particularly in Sukra, Rawamerta, East Karawang, Patokbeusi, and Ciasem. After purification, DNA concentrations reduced to 6.12-37.45 µg/mL. Spectrophotometric measurements revealed higher purity, with A260/A280 ratios of 1.43-2.31 and A260/A230 ratios of 0.04-0.30. The 1,931 bp MvALS1 gene was successfully amplified across all ecotypes, demonstrating the purification method's efficacy in yielding DNA appropriate for molecular biology applications.
Uji Antagonisme Cendawan Endofit dari Daun Pegagan terhadap Colletotrichum capsici Penyebab Busuk Buah Cabai Shalma Friska; Untung Santoso; Noorkomala Sari
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2026): October 2026
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.12882

Abstract

Hasil panen cabai rawit di Indonesia mengalami penurunan akibat serangan hama serta infeksi penyakit. Salah satu hambatan utama dalam budi daya cabai adalah munculnya penyakit antraknosa yang ditimbulkan oleh Colletotrichum spp. Upaya penanggulangan penyakit ini umumnya dilakukan dengan pemakaian pestisida secara berulang, yang pada akhirnya menimbulkan dampak negatif terhadap ekosistem. Alternatif pengendalian yang lebih aman bagi alam adalah dengan penerapan agensia hayati. Salah satu bentuk penerapan agensia hayati dapat dilakukan melalui pemanfaatan cendawan endofit. Jenis tanaman inang yang sesuai untuk mendukung keberadaan cendawan endofit salah satunya adalah tanaman obat. Dengan demikian, riset ini ditujukan untuk mengeksplorasi kemampuan endofit dari salah satu tumbuhan berkhasiat obat yakni daun pegagan air, yang berfungsi sebagai agen antagonis terhadap patogen Colletotrichum capsici pada tingkat laboratorium serta menelaah sejauh mana cendawan endofit mampu menghambat pertumbuhan patogen Colletotrichum capsici. Berdasarkan Hasil penelitian, cendawan endofit daun pegagan air memiliki daya hambat terhadap patogen Colletotrichum capsici dengan mekanisme overgrowth by antagonis pada cendawan endofit EnDa8H dengan persentase hambat 60,50% dan EnDa12P dengan persentase 53,50% tetapi pada EnDa1P terjadi mekanisme mutual slight inhibition dengan persentase hambat 47%.
A Systematic Review of Apical and Basal Complex Roles in Toxoplasma gondii Invasion and Egress Suryadi Islami; Ridwan Hardiansyah; Fahmi Ilham Hatimi; Norbertus Marcell Prayogi; Rais Amaral Haq
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2026): October 2026
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.13931

Abstract

Toxoplasma gondii is an obligate intracellular protozoan parasite of major medical and veterinary importance worldwide. Successful infection depends on its ability to actively invade host cells, replicate intracellularly, and egress to infect new cells. These processes involve complex molecular coordination among specialized organelles that ensure parasite survival and propagation. In the past decade, numerous experimental studies employing advanced molecular and high-resolution microscopy techniques have revealed new insights into the structural and functional dynamics of T. gondii, highlighting the need to systematically review and integrate these findings. This systematic review aims to identify, synthesize, and analyze experimental studies related to the molecular mechanisms of T. gondii infection, including host cell invasion, intracellular replication, and egress. Relevant literature published between 2015 and 2025 was retrieved from the Scopus and PubMed database using specific keywords. Articles were screened based on predefined inclusion and exclusion criteria, and the selection process followed the PRISMA guideline. From an initial 44 articles, 14 met the eligibility criteria after title and abstract screening, and 10 experimental studies were included for full-text analysis. Methodological quality and risk of bias of the included studies were evaluated using the Quality Assessment Tool for In Vitro Studies (QUIN) and visualized using the robvis tool. The systematic synthesis of these findings provides an integrated understanding of the molecular basis of T. gondii invasion and egress.
Keanekaragaman Avifauna dan Analisis Potensi Gangguannya di Area Gardu Induk PLN Medari, Sleman, Yogyakarta Donan Satria Yudha; Salsabila Putri Nirmala
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2026): October 2026
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i3.14144

Abstract

Gardu induk berperan dalam distribusi listrik untuk memenuhi kebutuhan listrik masyarakat dan terkadang mengalami gangguan internal maupun eksternal. Salah satu gangguan yang muncul berasal dari avifauna. Penelitian ini bertujuan untuk mempelajari keanekaragaman spesies avifauna yang ada di area Gardu Induk PLN Medari, menganalisis potensi gangguan yang ditimbulkan avifauna di area Gardu Induk, serta memahami potensi bentang alam sebagai habitat avifauna yang berada di area Gardu Induk tersebut. Metode yang digunakan adalah point count pada tujuh titik sampling yang telah ditentukan, yaitu titik di area Gardu Induk PLN Medari dan bentang alam di sekitar lokasi utama (area persawahan dan zona riparian). Pengambilan data dilakukan pada musim kemarau dan musim penghujan, dengan tiga kali pengulangan pada tiap sampling. Data avifauna kemudian diidentifikasi, ditabulasi, serta dianalisis dengan Indeks Dominansi Simpson dan Matriks Penilaian Risiko. Hasil menunjukkan terdapat 12 spesies avifauna yang dijumpai di Gardu Induk PLN Medari, dengan 9 spesies diantaranya ditemui pula pada inventarisasi tahun 2024. Keduabelas spesies tersebut juga dijumpai di titik bentang alam sekitar Gardu Induk. Bentang alam tersebut berperan sebagai habitat dan tempat mencari pakan spesies avifauna. Gangguan dari 12 spesies avifauna pada Gardu Induk dikategorikan mulai dari ekstrem (sangat mengganggu) hingga rendah (tidak mengganggu).

Page 122 of 123 | Total Record : 1224

 119   120   121   122   123 

Filter by Year

2003 2026


Reset
Filter By Issues
All Issue Vol 11, No 3 (2026): October 2026 Vol 11, No 2 (2026): June 2026 Vol 11, No 1 (2026): February 2026 Vol 10, No 3 (2025): October 2025 Vol 10, No 2 (2025): June 2025 Vol 10, No 1 (2025): February 2025 Vol 9, No 3 (2024): October 2024 Vol 9, No 2 (2024): June 2024 Vol 9, No 1 (2024): February 2024 Vol 8, No 3 (2023): October 2023 Vol 8, No 2 (2023): June 2023 Vol 8, No 1 (2023): February 2023 Vol 7, No 3 (2022): October 2022 Vol 7, No 2 (2022): June 2022 Vol 7, No 1 (2022): February 2022 Vol 6, No 3 (2021): October 2021 Vol 6, No 2 (2021): June 2021 Vol 6, No 1 (2021): February 2021 Vol 5, No 3 (2020): October 2020 Vol 5, No 2 (2020): June 2020 Vol 5, No 1 (2020): February 2020 Vol 4, No 3 (2019): October 2019 Vol 4, No 2 (2019): June 2019 Vol 4, No 1 (2019): February 2019 Vol 4, No 1 (2019): February 2019 Vol 3, No 3 (2018): October 2018 Vol 3, No 2 (2018): June 2018 Vol 3, No 1 (2018): February 2018 Vol 3, No 1 (2018): February 2018 Vol 2, No 3 (2017): October 2017 Vol 2, No 2 (2017): June 2017 Vol 2, No 1 (2017): February 2017 Vol 2, No 1 (2017): February 2017 Vol 1, No 3 (2016): October 2016 Vol 1, No 2 (2016): June 2016 Vol 1, No 1 (2016): February 2016 Vol 1, No 1 (2016): February 2016 Vol 19, No 1 (2014): February 2014 Biota Volume 19 Nomor 1 Tahun 2014 Biota Volume 13 Nomor 2 Tahun 2014 Vol 18, No 2 (2013): June 2013 Vol 18, No 1 (2013): February 2013 Biota Volume 18 Nomor 1 Tahun 2013 Vol 17, No 3 (2012): October 2012 Vol 17, No 2 (2012): June 2012 Vol 17, No 1 (2012): February 2012 BIOTA Volume 17 Nomor 3 Tahun 2012 Vol 16, No 2 (2011): June 2011 Vol 16, No 2 (2011): June 2011 Vol 16, No 1 (2011): February 2011 Vol 16, No 1 (2011): February 2011 Vol 15, No 3 (2010): October 2010 Vol 15, No 2 (2010): June 2010 Vol 15, No 1 (2010): February 2010 Vol 14, No 3 (2009): October 2009 Vol 14, No 2 (2009): June 2009 Vol 14, No 1 (2009): February 2009 Vol 13, No 3 (2008): October 2008 Vol 13, No 2 (2008): June 2008 Vol 13, No 1 (2008): February 2008 Vol 12, No 3 (2007): October 2007 Vol 12, No 2 (2007): June 2007 Vol 12, No 1 (2007): February 2007 Vol 11, No 3 (2006): October 2006 Vol 11, No 2 (2006): June 2006 Vol 11, No 1 (2006): February 2006 Vol 10, No 3 (2005): October 2005 Vol 10, No 2 (2005): June 2005 Vol 10, No 1 (2005): February 2005 Vol 9, No 3 (2004): October 2004 Vol 9, No 2 (2004): June 2004 Vol 9, No 1 (2004): February 2004 Vol 8, No 3 (2003): October 2003 Vol 8, No 2 (2003): June 2003 Vol 8, No 1 (2003): February 2003 More Issue