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Pore Size Distribution and Microstructure of Oil Palm Shell Heat Treated at 300 C Followed by Slow or Fast Heating Treatment Joko Sulistyo; Toshimitsu Hata; Yuji Imamura; Purnomo Darmaji; Sri Nugroho Marsoem
Wood Research Journal Vol 9, No 1 (2018): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2018.9.1.15-25

Pore size distribution and microstructure development of oil palm shell heat treated at 300ºC and treated at 300ºC and recarbonization at 600ºC followed by slow- or fast heating treatment up to 700ºC were investigated by small angle X-ray scattering (SAXS), N2 gas adsorption and Raman spectroscopy. On oil palm shell heat-treated at 300ºC, slow heating treatment gave the widening micropore along with the ordering microstructure; but fast heating treatment produced charcoal with a narrow diameter of micropore with wider pore size distribution and the disordering microstructure. On oil palm shell heat treated at 300ºC and recarbonization at 600ºC, slow heating treatment contributed on the opening new micropore with ordering microstructure, but some parts of micropore showing inaccessible for N2 gas. Meanwhile, fast heating treatment with the heating rate from 75 to 250ºC/min increased BET surface area with similar pore size distribution and the disordering microstructure.

Pore Size Distribution and Microstructure of Oil Palm Shell Heat Treated at 300 C Followed by Slow or Fast Heating Treatment Joko Sulistyo; Toshimitsu Hata; Yuji Imamura; Purnomo Darmaji; Sri Nugroho Marsoem
Wood Research Journal Vol 9, No 1 (2018): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2018.9.1.15-25

Pore size distribution and microstructure development of oil palm shell heat treated at 300ºC and treated at 300ºC and recarbonization at 600ºC followed by slow- or fast heating treatment up to 700ºC were investigated by small angle X-ray scattering (SAXS), N2 gas adsorption and Raman spectroscopy. On oil palm shell heat-treated at 300ºC, slow heating treatment gave the widening micropore along with the ordering microstructure; but fast heating treatment produced charcoal with a narrow diameter of micropore with wider pore size distribution and the disordering microstructure. On oil palm shell heat treated at 300ºC and recarbonization at 600ºC, slow heating treatment contributed on the opening new micropore with ordering microstructure, but some parts of micropore showing inaccessible for N2 gas. Meanwhile, fast heating treatment with the heating rate from 75 to 250ºC/min increased BET surface area with similar pore size distribution and the disordering microstructure.

Aktivitas Antimutagen Isoflavon Glikosida Hasil Transglikosilasi Enzimatik CGT-ase Bacillus macerans Joko Sulistyo; Yati Sudaryati Soeka
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i1.2629

It has been known that isoflavone have biological activities such as antioxidant, antibacteria, antimutagenesis, and anticancer. Isoflavone aglycone uses such as genistein, daidzein and glycitein are limited since they are unstable and uneasily to dissolve in water. Through enzymatic transglycosylation reaction, its stability and solubility could be improved. In this study, genistin (isoflavone glycoside) was synthesized from genistein (isoflavone aglycone) by application of transfer reaction using enzyme cyclodextrin glucanotransferase (CGT-ase) of Bacillus macerans. Identification of the product was determined by TLC with methanol: chloroform (1:3, v:v) as eluent. Rf value 0.75 of the synthesized product was close to the Rf value standard of authentic genistin glycoside. The synthesized genistin was furthermore assayed to determine its antimutagenesis activity according to the Ames methode on E. minimal glucose media had been precultured with a mutant strain of Salmonella thypimurium TA98. The tested bacterial strain was induced with aflatoksin B1 as mutagen which had been activated with a lever homogenate. The result showed that the solubility and some biological properties of the synthesized genistin were improved higher than that of genistein, while it was found to be lower than that of the commercial genistin.

Biotransformasi Pirokatekol Glikosida Menggunakan Kultur Suspensi Sel Solanum mammosum L. Yati Sudaryati Soeka; Joko Sulistyo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2704

A syntesis of pyrocathecol glucoside was carried out by applying biotransformation cell culture suspension from calus of Solanum mammosum L., on modified medium of Murashige and Skoog (MS). A growth maximum volume at 15,5 ml of cell culture suspension of S. mommosum was achieved on day-8 incubation. The results showed that pyrocathecol glucoside as a bitransformation product that was obtained by application of Pyrocathecol at 50-200 ppm was determined by TLC and identified at Rf value of 0.82−0.83. Futhermore, the biotransformation products were determined by HPLC obtained from the cell culture suspension at concentration of 200 ppm pyrocathecol so that resulted in reaction products based on standard solution. The peaks number 1, 2 and 3 with retention time 2.53 min, 4.62 min and 7.58 min were appropriate to the retention time cellobiose, glucose and methyl α-glucoside, respectively. Peak number 4 with retention time 8.52 min conformed to pyrocathecol-glucobioside as a product of side transfer and peak number 5 with retention time 10.52 min in line with the retention time of arbutin were pyrocathecol-glucoside as a transfer product expected from the result of biotransformation.

ANALISA ASAM ORGANIK DAN ASAM AMINO PADA NATTO YANG DIFERMENTASI OLEH 7 STRAIN BACILLUS NATTO JOKO SULISTYO
BERITA BIOLOGI Vol 3, No 6 (1986)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i6.1326

JOKO SUUSTYO. 1986. Analysis of organic acid and amino acid in natto fermented by 7 strains of Bacillus natto. Berita Biologi 3 (6): 277 - 282. The ability of seven strains of Bacillus natto produced a number of organic and amino acid components in natto were investigated. Raw, submerced and autoclaved soybeans for natto contained 1.15% 0.46% and 0.77% in organic acid and 0.19%, 0.30% and 0.30% in amino acid, while after fermentation those range were 0.36 - 0.90% in organic acid and 2.86 -6.08% in amino add. During submercing, autoclaving and fermenting, citric acid component decreased down very sharply to about one hundredth of starting level. While acetic,iso-butiric and iso-valeric components increased up to 84.92%, 96.60% and 95.87%, respectively. Mean while, glutamic acid, iso-leucine,leucine andphenylalanine components of amino acid increased up to 84.36%, 90.52%, 91.67% and 95.88%, respecti

PENERAPAN TEKNOLOGI FERMENTASI PADA BIOPROSES FERMENTASI MINYAK KELAPA (FERMIKEL) Joko Sulistyo; Yati Sudaryati Soeka; Evi Triana; Rostiati NR Napitupulu
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1246

Methods of extracting oil from coconut endosperm by fermentatbn were studied. The factors which must be controlled to break the emulsion and liberate oil were investigated. It was found that grinding conditions exerted a profound effect upon the stability of the coconut milk emulsion. The optimum condition for rapid fermentathn of coconut milk was related to the condition during incubation period. The fermentation progressed best under mild conditions (28°C-40°Cj. The fermentation was successful in breaking the emulsion at a relatively broad of range and titrable acidity. Coconut cream and small volume of coconut water and "lontar" (palmyra palmj-sap were incubated separately with some strains of Bacillus species, which were preincubated in a coconut tomato-extract sugar (CTSj medium using a shaker, and grown as a starter under conditions that allowed for coconut oil production at pH 4,0-5,0 and 30 C°- 40 "C for 12-24 h. The organism destabilizes the emulsion, apparently by metabolizing sugars, resulting in the production of protein curd and high-quality oil. The palm sap and coconut water to the cream ratio of fermentation medium influenced the performance of oil produced and the bacteria grew well and produced oil in non sterile systems. The oil recovered was about 25 to 20% while average amount of oil in the coconut is approximately 25-35%, which means that only 83,33 to 66,67% oil was recovered. The oil contained little free fatty acid and very low concentration of cholesterol (0,0095 mg/ml), while the traditional coconut oil and commercially palm oil were 0,0111 mg/ml and 0,0132 mg/ml, respectively.

SEMI PURIFIKASI DAN UJI REAKSI TRANSGLUKOSILASI P-GLUKOSIDASE DARI Aspergillus pulverulentus Joko Sulistyo; Yati Sudaryati Soeka; Purnama Dewi
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1263

An extracellular p-glucosidase[EC 3.2.1.21]derived from Asvereillus vulverulentus were separated and partially purified by successive chromatographies and its some characterization and transglucosylation capacity were studied.The purification protocol included precipitation with ammonium sulphate,gel filtration,ion exchange chromatography and native polyacrylamide gel electrophoresis.The enzyme readily hydrolysed cellobiose to form transglucosylation products in the present of primary alcohol acceptors.This P-glucosidase was stable at temperatures up to 70 °C and from pH 2.5 to 8.5, while its highest activity was in the pH 4.5 at 65°C.

Physical and Mechanical Properties of 10-Year Old Superior and Conventional Teak Planted in Randublatung Central Java Indonesia Fanny Hidayati; Joko Sulistyo; Ganis Lukmandaru; Tomy Listyanto; Harry Praptoyo; Rini Pujiarti
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 13, No 1 (2015): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Tree breeding program has been conducted in Indonesia in order to produce more productive teak trees. Recently, from this program, superior clones (clone source) of teak have been selected for the establishment of the wider plantations. These clones show a good performance on growth characteristics such as stem diameter and tree height. However, it is important to evaluate wood quality of selected superior teak. Physical (heartwood percentage, wood color, basic density, and shrinkage per 1% change in moisture content) and mechanical (static bending strength and compressive strength) properties were investigated for 10-year old of two sources type of teak (superior and conventional) planted in Randublatung, Central Java, Indonesia. There was not significant different between superior and conventional teak was found in all physical and mechanical properties of both teak wood, suggesting that wood properties of both teak are similar at the same age. All trees are in juvenile phase as show by increasing of basic density from pith to bark. Basic density has positively correlation with all mechanical properties measured. It can be said that basic density can be used to estimate mechanical properties.Keywords: conventional teak, mechanical properties, physical properties, superior teak

Characteristic of Oyster Mushroom Grown on Substrates Containing Paper Waste Joko Sulistyo; Soh Chin Quan; Lee Khaa Hwee
Bionature Vol. 24 No. 2 (2023): Oktober 2023
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

The aim of study was to determine the capacity of spawn of oyster mushroom and influence of substrates containing PW used to grow the mushroom and its properties related to nutritional value and antioxidant activity due to the use of paper waste (PW). The substrate was prepared based on 5 different formulations which were 0% (control), 20%, 30%, 40%, 50% PW. Preparation of seeds and spawn and mushroom cultivation technique was carried out manually using basic techniques of mushroom seeding and cultivation, while proximate analysis was carried out to analyze the content of fat, protein, moisture, and ash of the mushroom fruiting bodies, followed by analysis of heavy metals using AAS, and furthermore carried out extraction of bioactive compounds to analyze antioxidant activity using ABTS oxidizer. The physicochemical analysis was carried out to test the quality of the mushroom. Overall, the mushroom grown on the substrates containing 50% PW showed higher content of ash, protein, fat and crude fiber compared to the mushroom grown on other substrates with different composition of PW. However, the mushroom grown on the substrate contained 50% PW indicated considerably high content of heavy metals such as lead, (3.5%), copper (20.8%) and cadmium (4.4%), respectively, although, it showed the higher antioxidant activity (87.19%) as well compared to the mushrooms grown on the substrate containing a lower percentage of PW, thus giving a conclusion that there was probably positive correlation in between the heavy metals contained in PW with antioxidant activity.

Pengaruh Substitusi Cuka dengan Sari Buah Delima terhadap Karakteristik Fisikokimia, Kualitas Sensoris, dan Aktivitas Antioksidan Mayones Jennifer Callysta Thee; Nyoman Puspa Asri; Joko Sulistyo
Jurnal Riset Rumpun Ilmu Tanaman Vol. 4 No. 1 (2025): April : Jurnal Riset Rumpun Ilmu Tanaman
Publisher : Pusat riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrit.v4i1.5143

This study aimed to evaluate the effect of substituting vinegar with pomegranate juice (PJ) on the physicochemical characteristics, sensory quality, and antioxidant activity of mayonnaise. The mayonnaise was formulated into five treatments with varying concentrations of vinegar and PJ. The analyzed parameters included pH, color (L*, a*, b*), viscosity, emulsion stability, and sensory attributes (taste, aroma, color, texture, and preference), while antioxidant activity was assessed in the best formulation. The results showed that increasing the PJ substitution significantly improved viscosity and emulsion stability, decreased lightness (L*), and enhanced the red hue (a*). The P4 formulation (containing 2.75% PJ) demonstrated the best overall performance, with high viscosity, excellent emulsion stability, and also receiving high sensory scores. Antioxidant analysis revealed that P4 had higher antioxidant activity compared to the control (P0). Therefore, PJ has the potential to serve as a natural alternative to vinegar in mayonnaise production while enhancing the product's stability and functional value.

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