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JURNAL PEMULIAAN TANAMAN HUTAN

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Published by Badan Penelitian dan Penegembangan Kehutanan

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Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

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Articles 249 Documents

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PEMBENTUKAN KALUS REMAH DARI EKSPLAN DAUN RAMIN (Gonystylus bancanus (Miq) Kurz.) nfn Yelnititis
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 3 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.3.181-194

Ramin (Gonystylus bancanus (Miq.) Kurz) is the most favourite and the most over exploited woody species. This species has been listed in CITES APPENDIX II from 2004. The purpose of this experiment is to obtain the best treatment for friable and embryogenic callus formation that can develop to somatic embryo. Basal Murashige and Skoog (MS) media was used as growth medium. The experiment was conducted in three stages : callus induction and propagation stage, friable callus induction stage and embryogenic callus induction stage. The treatment of 3.0 – 5.0 mg/l 2,4-D was used for callus induction. The best of callus was propagated in 5.0 mg/l 2,4-D + 1.0 – 2.0 mg/l thidiazuron. The best of callus was subcultured for callus friable induction used 6.0 mg/l 2,4-D + 1.0 – 2.0 mg/l thidiazuron + 1.0 – 2.0 mg/l biotin. The best friable callus was subcultured for embryogenic callus induction used 7.0 – 8.0 mg/l 2,4-D + 1.0 – 2.0 mg/l biotin. The observation was made on texture, percentages, performance and color of friable callus. The results showed that callus can be induced on 5.0 mg/l 2,4-D. The treatment of 6.0 mg/l 2,4-D + thidiazuron produced friable callus yellowish on color. 2,4-D + biotin treatment produced very friable callus with yellowish on color and with no embryogenic callus development.

PENGEMBANGAN PENANDA MIKROSATELIT PADA Pinus merkusii MENGGUNAKAN METODE DUAL SUPRESSION DAN SCREENING PENANDA ILG Nurtjahjaningsih
Jurnal Pemuliaan Tanaman Hutan Vol 4, No 1 (2010): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2010.4.1.25-35

Aims of this study were to develop and characterize microsatellite markers from three species of Pinus i.e P. densiflora, P. Pinaster and P. Taeda were screened to amplify microsatellite in P. merkusii. The results showed that 5 out of the 12 developed microsatellite markers by dual suppression amplified at a single polymorphic locus; otherwise there was no polymorphic locus of the 12 screened microsatellite markers. However, it is difficult to compare the efficiency of these two method because of the limited number of the screened primers used in this study.

Ponngamia pinnata- Rhi zobium: SPESIES SIMBIOTIK BERNILAI KONSEVASI TINGGI Asri Insiana Putri
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 2 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.2.73-81

Pongamia pinnata is an arboreal legume with high conservation value (Conference of The Parties to The Convention on Biological Diversity at the IX/2 nd on plants of high conservation volue criteria). Biotechnology breeding program with the selection of legume crops that have a high nitrogen use efficiency becomes an important issue to improve productivity and quality of P. pinnata.Thus the important symbiotic effectiveness test done to improve the ability of N fixation as the main element forming a plant tissues . The purpose of this study was to obtain Rhizobium strain from different P. pinannta habitats which has the highest effectiveness compared with strain Rhizobium sp. Commercial (USDA 122) and controls to P. pinnata seedlings. The parameters used are the number of nodules, plant height, root length and diameter . From this activity obtained 3 strains of Ambon and 2 strains of Banyuwangi . Strains selected on the basis of colony growth speed of Rhizobium sp.in vitro.The average number of nodules, plant height, root length and diameter of P. pinnata that are inoculated with strain AMI. AM2, AM3, Bal and Ba2 from seram, Ambon higher than from Baluran , Banyuwangi. On average, the highest number of nodules occurred in simbiose of P. pinnata) Ambon Rhizobium AM3 (50 ± 0,66) eguels P. pinnata Ambon –Rhizobium USDA 122 (50 ± 0,88); the best plant height occurred in P. pinnata Ambon-Rhizobium Ba2 (72cm ± 1,14) ; the best root length (25 cm ± 0.33) and the best stem diameter (4.0 mm ± 0.33) occurred in P. pinnata Ambon-Rhizobium AM2. The highest symbiose effectiveness value of P. pinnata-Rhizobium occureed in P. pinnata origin from Ambon with Rhizobium strain AM3 (32,45).

PENGARUH JENIS DAN KONSENTRASI ZAT PENGATUR TUMBUH PADA INDUKSI KALUS EMBRIOGENIK KLON CENDANA (Santalum album Linn.) Toni Herawan; Mohammad Na'iem; Sapto Indrioko; Ari Indrianto; Liliek Haryjanto; Titis Budi Widowati
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 2 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Sandalwood (Santalum album Linn.) is a tree which has a high rate of natural sprouting ability. Eventhough the propagation by the conventional techniques (shoot and root cuttings) and by the tissue culture have been reported, the percentage of plants regeneration is still low. The propagation using somatic embryogenesis was reported as better result than using shoots multiplication technique or organogenesis. The objective of this research is to examine the effect of clones, type and concentration of plant growth regulator on the development embryogenic callus of sandalwood. The three tested clones are C1, C2, and C3. The plant growth regulators are 2.4-D, Dicamba, and Picloram with the three level of concentrations:1 mg/L, 3 mg/L, and 5 mg/L. The result of study showed that the clone of C3 performed best on embryogenic callus development. It was observed through morphological analysis that 58.12% of explants performed embryogenic callus with friable texture and white, yellowish in colours. However, there were not significant differences between the types of plant growth regulator, the level of concentrations and their interactions on embryogenic callus development of sandalwood

THE EFFECT OF ORGANIC MEDIA ON THE QUALITY OF Santalum album SEEDLING Asri Insiana Putri
Jurnal Pemuliaan Tanaman Hutan Vol 2, No 1 (2008): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2008.2.1.139-148

The quality of Santalum album seedling is influenced by growing media where their root grow and develop. This research was conducted to confirm that organic growing media is a good characteristics as top soil media and to determine the quality index value as indicator of seedling quality. There types of media; top soil, composted sawdust and pure sawdust were arranged in a completely randomized design with 4 replicates of 5 seeds. The observation periode was 8 month, from January until September 2007. The resuly showed that index value for S, album seedling in organic media is as relatively high as in top soil media; 1,42 for top soil, 1,10 for composted sawdust and 1,08 for pure sawdust.

MORFOLOGI PEMBUNGAAN DAN SISTEM REPRODUKSI MERBAU (Intsia bijuga) PADA PLOT POPULASI PERBANYAKAN DI PALIYAN, GUNUNGKIDUL Liliana Baskorowati; Sugeng Pudjiono
Jurnal Pemuliaan Tanaman Hutan Vol 9, No 3 (2015): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Information regarding the flowering morphology, as a first step to understand the reproductive system, is essential. The purpose of this study is to determine the flowering morphology and the reproductive systems of merbau. Observation of flowering and fruiting intensity was undertaken every week for 6 consecutive months, from May to November 2013. Observations of the development of generative organs were carried out on daily basis to determine the duration and the length of each development stage of flowers and fruits. Results showed that flowering of merbau is arranged in spikes, hermaprodite with un synchronous flowering between and within spikes. Reproductive organs are protandry and apparently heterostyly type, indicating that self-incompatibility may occur in this species. None fruit was formed from self-pollination experiment; supporting the allegation of crosspollination systems (xenogamy). Flowering occurs twice a year with peak flowering in June and November, followed by fruit maturation in the next 3 months. Various types of insect visitors found during this study, however very few insect can be determined as pollinators. Flowers and pollinator limitation lead to the mechanism of self-incompatibility causing low reproductive success on this species. Therefore, an artificial cross-pollination or an introduction of pollinators needs to be done in order to enhance the reproductive success of this species.

IDENTIFIKASI TIGA ISOLAT CENDAWAN PENGHASIL GAHARU DARI NUSA TENGGARA BARAT DENGAN MENGGUNAKAN PRIMER ITS DAN TEF 1-α Y.M.M Anita Nugraheni; Lutfi Anggadhania; Riza Arief Putranto
Jurnal Pemuliaan Tanaman Hutan Vol 9, No 2 (2015): Jurnal pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Several isolates of agarwood-forming fungus in Gyrinops versteegii have been isolated from the result of exploration in Lombok and Sumbawa Islands. This study aimed to identify the three fungus associated with the agarwood formation in G. versteegii originated from Lombok Tengah, Alas, and Lombok Barat, Nusa Tenggara Barat. The three fungus cultured in liquid medium PDB (Potato Dextrose Broth) and incubated for 1 month in shaken culture. The mycelium of each fungus was harvested for DNA isolation. Amplification is done by Polymerase Chain Reaction (PCR) using the Primers ITS and TEF with obtained amplicon having base length ranging of 300-600 bs. BLAST analysis showed that the three fungus have similarity with Fusarium solani. These results were confirmed by phylogenetic tree where all fungus has genetic relationship with F. solani.

RESPON POPULASI Eucalyptus occidentalis TERHADAP SELEKSI KONDISI GARAM TINGGI PADA UJI TERKONTROL DAN UJI LAPANGAN Rina Laksmi Hendrati
Jurnal Pemuliaan Tanaman Hutan Vol 4, No 2 (2010): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2010.4.2.91-100

Global changes together with rising number of human population, industrial development, and exploitation of natural vegetation, are the main factors causing high salinity in many regions of the world. Trees tolerant to grow in saline regions are crucially needed and can be used as an effective means to control or even decrease salinity. Eucalyptus occidentalis, a Western Australia native tree spesies that is highly tolerant to salt has a wide range of natural habitats from waterlogged, highly saline to arid regions. Selections of provenances and families from throughout its natural distribution are needed to identify the most tolerant genotypes within this species. Comparative performances of E. occidentalis under salt and/or waterlogged conditions were investigated under controller condition and in field trial. An initial screening of 25 provenances was conducted for salt tolerance ranging from 0 mM to 300 mM under control, waterlogged, dry salt and salt treatments. Nine provenances selected from the first screening were exposed to similar treatments up to 550 mM of salt concentration. The same seed collections of 23 provenances were germinated at the same time and planted in field trial in Roberts, South Western Australia. Salt concentration of 300 mM did not affect E. occidentalis growth, and therefore it is not an appropriate level for selection. Under 550 mM of salt concentration, plants were depressed and differences between provenaces are much more noticeable. There were different responses of E. occidentalis populations on selection under high salinity between controlled condition and in field trial.

PERTUMBUHAN JABON (Anthocephalus cadamba Miq.) PADA LAHAN MARGINAL BERJENIS TANAH ULTISOL DI RIAU Ahmad Junaedi
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 1 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Study on growth of jabon (Anthocephalus cadamba Miq) as native tree species that suitable for pulpwood on ultisol-soil land is required, as most pulpwood plantation occur in this type of soil. The experiment was conducted in ex Acacia mangium (second rotation) in Riau to evaluate the growth performance of jabon on marginal land ultisol soil. This study assigned experiment plots of jabon with three planting spaces (2 m × 2 m; 2 m × 3 m and 3 m × 3 m) and four blocks in Randomized Block Design. The poor growth was exhibited by jabon on marginal land ultisol. It was suggested that the poor growth related to the negative effects of low N and P soil, high Al soil, and threats of pest, disease, weed as well. The result study indicated that jabon was not suitable to be developed as pulpwood species in marginal lands ultisol of pulpwood plantation in Riau.

COTYLEDON MEASURE INFLUENCE TO GROWTH OF ULIN (Eusyderoxylon zwageri T. Et B.) SEEDLING Lukman Hakim; Mohamad Anis Fauzi
Jurnal Pemuliaan Tanaman Hutan Vol 2, No 1 (2008): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2008.2.1.173-179

Ulin (Eusideroxylon zwageri) has much bigger seed compared to others tree species. This condition poses some difficulties in seed collecting and seed transporting from seed collecting area to nursery. The aim of this research is to examine the correlation between Ulin seed length with stem and root length in nursery. The study used 4 provenances and 15 seeds per provenance. Regresion analyzes showed that seed length variable (X) versus stem length (Y1) has formula Y1= 9,601 + 0,446 X and seed length variable (X) versus root length (Y2) has formula Y1= 5,884 + 0,428 X. From both formula showed that seed length variable (X) are not significant to stemp length and root length variable.

Page 7 of 25 | Total Record : 249

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