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JURNAL PEMULIAAN TANAMAN HUTAN
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Core Subject : Agriculture,
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Articles 249 Documents
KARAKTERISTIK PERTUMBUHAN JABON DARI PROVENAN SUMBAWA PADA TINGKAT SEMAI DAN SETELAH PENANAMAN Tri Pamungkas Yudohartono
Jurnal Pemuliaan Tanaman Hutan Vol 7, No 2 (2013): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2013.7.2.85-96

Abstract

Growth is one of important and economic traits of jabon. Growth is not only determined by environment but also genetic factor. Seedling quality and suitable site would create well growing plants. This research is purposed to know growth characteristic of jabon from Sumbawa  provenances in nursery and after plantation as well as the effect of genetic factor on growth characteristic of jabon. Research design used in nursery is Completely Randomized Design with 24 mother trees and 3  replications. Each replication comprises 10 seedlings. Totally, there are 720 seedlings. Experimental design used in progeny test plot of jabon is Randomized Complete Block Design. Breeding strategy used in this plot was subline system with single treeplot. Number of families used are 28 families. Each family comprises 20 blocks that were also functioned as replication. The results showed that there were significant differences in terms of height and diameter among the tested families at all observation ages. Family heritability estimates for height were high at at all observation ages. Family heritability estimates for diameter were high at nursery and moderate after plantation.
PENGARUH SELEKSI TERHADAP PEROLEHAN GENETIK PADA UJI KETURUNAN GENERASI PERTAMA (F-1) JABON MERAH (Anthocephalus macrophyllus (Roxb.) Havil) DI WONOGIRI Surip Surip; Sapto Indrioko; Arif Nirsatmanto; Teguh Setyaji
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.608 KB) | DOI: 10.20886/jpth.2017.11.1.33-44

Abstract

Selection is a fundamental process in the establishment of a progeny trial that will be converted into a seedling seed orchard. In present study, selection practiced in first-generation progeny trial of jabon merah (Anthocephalus macrophyllus Roxb. Havil.) was observed. The study was aimed to find out a criteria selection accompanied with the effective coefficient weight for predicting genetic gain in the first-generation progeny trial of jabon merah. The trial was established in Wonogiri which was laid out in randomized complete block design (RCBD) using 55 families, 4 replications, 4 tree-plot with a spacing of 4 x 2 meter. Measurement was conducted at 40 months after planting covering tree height, diameter at breast height, stem straightness, stem volume. First within-plot selection by culling 2 poorer trees within each plot was practiced at 46 months age. Selection differential from within-plot selection was used to derive coefficient weight which was then applied to predict genetic gain. Results of study showed that selection differential from first within-plot selection were positive for all measured traits. Stem volume was observed as highest priority trait with coefficient weight for each trait were -0.0863 (height), 0.1426 (diameter), 0.2195 (stem straightness), 2.3025 (stem volume). Prediction of genetic gain from within-plot selection were 3,50% (height), 3.58% (diamater), 3.41% (stem straightness), 7.40% (stem volume). While the gain from family selection were 2.80%, 3.75%, 3.54%, 7.10% for height, diamater, stem straightness, stem volume, respectively.
IDENTIFIKASI KLON JATI (Tectona grandis Linn F.) MENGGUNAKAN PENANDA SCAR (Seguence Characterized Amplified Region). Vivi Yuskianti
Jurnal Pemuliaan Tanaman Hutan Vol 3, No 3 (2009): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2009.3.3.139-146

Abstract

An easy, Cheap and acacurate identification method is needed to ensure the identity of teak clones used in planation. Molecular markers offer an alternative technique for clonal identivication . one such method is SCAR markers. Seventy teak clones of which 20 clones were produced by tissue cultures and claimed to be originated from the some parent. And 50 clones from buddings were used as materials. Analysis was conducted using 6 highly polymorphic SCAR primers. The results showed that 20 teakclones Originated from tissue cultures clustered into 3 groups, Whereas the rest 50 samples from buddings clustered into 4 groups. SCAR markers were able to differentiate the identity of these clones.These SCAR markers can applied to prove the claim abaout the identity of chones in the market and to ovoid labeling errors in the field  for Vegetatively propagated plants.
KARAKTERISASI KERAGAMAN GENETIK POPULASI JABON PUTIH MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA ILG Nurtjahtjaningsih; Maryatul Qiptiyah; Tri Pamungkas Yudohartono; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 8, No 2 (2014): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2014.8.2.81-92

Abstract

Anthocepalus cadamba (white jabon) has high economical value for furniture. White jabon forests severely degraded due to intensive exploitation and land conversion. Genetic diversity is one of important consideration to design conservation and improvement strategies. Aim of this study was to access the genetic diversity values within and among population of white jabon. Leaf samples of white jabon were collected from conservation plots originated from West Lombok, Sumbawa, South Sumatera and West Sumatera. Red jabon was included as an outgroup population. Based on 37 polymorphic RAPD loci, the results showed comparable value of genetic diversity between white jabon and red jabon. Number of detected and rare alleles was highest founded in Sumbawa population among the other three populations of white jabon. As consequence, value of expected heterozygosity in the population was highest (HE=0.315). Private allele was only detected in South Sumatera population. Principal coordinate analysis (PCA) showed that integrating between genetic and geographical distance was inconsistent; similar gene resources or human impact might be responsible for this result. The populations that have high value of genetic diversity and private allele are recommended to be selected for the conservation strategies, i.e. Sumbawa and South Sumatera.
DEPRESI SILANG DALAM BIJI DAN SEMAI Melaleuca alternifolia Liliana Baskorowati
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 2 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.949 KB) | DOI: 10.20886/jpth.2017.11.2.87-98

Abstract

Tea tree (Melaleuca alternifolia) is known as an essential oil producer. This species is commercially important as a source of essential oil especially in Australia. To improve its oil quality, research on artificial pollination between plus trees were carried out. The information about self-incompatibility and inbreeding depression due to self-pollination should be ascertained to support the pollination success. This study was aimed at examining the self-incompatibility of tea tree by conducting controlled self-pollination and cross-pollination. Controlled cross and self-pollination were carried out at a seedling seed orchard of tea tree, using four mother trees as experimental samples. Natural self-pollination was undertaken by bagging nopened flowers, without emasculation and counting the number of capsule set. Open pollination was used as a control treatment, of which the number of unopened flowers and the number of capsule set were counted. Observations revealed that no capsule was found from controlled self pollination (with the index self-incompatibility = 0), even though natural self-pollination produced low number of capsules (with the index self-incompatibility = 0.24). Therefore, it can be assumed that high level of self-incompatibility was took place in tea tree. Inbreeding depression also existed in this species, revealed by the decreased number of capsul set, lower seed germination rate of self-pollinated seeds than cross pollinated seeds. The slower growth of self-pollinated seedlings than cross pollinated seedlings in the nursery also indicated that inbreeding depression occured.
THE EFFECT OF NUMBER ON AXILLARY BUDS ON THE SURVIVAL RATE AND GROWTH OF CUTTING OF FOUR MULBERY HYBRID Aris Sudomo; Sugeng Pudjiono; Mohamad Na'iem
Jurnal Pemuliaan Tanaman Hutan Vol 1, No 1 (2007): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2007.1.1.29-42

Abstract

The study aimed to examine the effect of number of axillary buds on different types of mulberry hybrid and their interaction on the survival rate and cutting growth. The research was conducted using Random Design of Factorial Group (RDFG). It consists of two treatment factors, i.e. four type of mulberry hybrid (M. SHA 4 x M. LUN 109, M. multicaulis x M. indica, M. australis x M. indica, M. nigra x M. indica) and four different numbers of axillary buds with 3 replications and 30 replicates for each unit experiment. The research shows that there were effect of axillary bud number, types of mulberry hybrid, and their interaction on the survival rate and growth of stem cutting of mulberry hybrid. The number of axillary bud for the best growth of stem cutting of mulberry hibrid for the best sprout length of cutting and the highest survival percentage (81, 668%) was the 3 axillary buds, while the 4 buds was the best according to the rank of whole parameter. The best cutting type of mulberry hybrid according to the rank of whole parameter was M. australis x M. indica. Interaction beetwen mulberry hybrid and the best sprout number according to the rank of whole parameter was M. australis x M. indica with 4 sprout number.
KARAKTERISTIK BUAH DAN MUTU MORFO-FISIOLOGIS BENIH LONKIDA (Nauclea orientalis L.) DARI HABITAT ALAMI DI SULAWESI TENGGARA Faisal Danu Tuheteru; Cecep Kusmana; Irdika Masyur; Iskandar Iskandar
Jurnal Pemuliaan Tanaman Hutan Vol 8, No 3 (2014): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2014.8.3.152-170

Abstract

Lonkida (Nauclea orientalis L.) is a native multipurpose tropical tree species in Indonesia, which a few information regarding its biology and ecology. An experimental research was carried out to examine characters of fruit, seed and seed quality of lonkida growing at different habitat in South East Sulawesi. Fruit and seed was collected from four habitat types, such as dry land, permanent swamp, temporary swamp and temporary swamp savanna. The parameters to be observed were fruit size, seed size and morpho-physiology seed quality. The results showed that fruit size and number of seed per fruit are significantly different among habitats. The mean of fruit’s length and width and number of seed per fruit originated from permanent swamp and temporary swamp is greater than that of fruit’s from lonkida stand growing in the remain habitat’s. Those parameters ranging from 4.99±0.17 to 6.66±0.19 cm for fruit length and 3812±519 to 13.984±1912 seed for number of seed per fruit. Beside, the habitat condition also significantly affected to the characters of seed dimension and seed quality. Permanent swamp was a habitat with seed size and seed weight lower compare to another habitats. However, seeds produced by the lonkida stand growing on permanent swamp habitat and temporary swamp savanna has the higher percent of germination, germination capacity and mean day germination (MDG) than that of the seeds from lonkida stand growing on other habitats. Seed length is positively correlated with seed width and negative correlation with means germination time.
THE USE OF FTA CARD ON DNA SAMPLE PREPARATION FOR MOLECULAR OF PLANT DISEASE IDENTIFICATION Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 1, No 3 (2007): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2007.1.3.123-130

Abstract

Accurate and guick identification of pathogen is key to control the spread of plant disesases. Morphological identification is often ineffective because  it requires fruit body which often are not presence, rely on characters which may be highly variable within and among species and can be slow and time consuming. Molecular identification of plant disease can overcome most of the shortcomings of morphological identification. Application of FTA Cardn for sample collection is crucial for the success of molecular identification of phatogen. The study present an earlier results on the suitability of FTA Card to collect sample in situ. The study was done on 4 different sample namely pure culture of fusarium sp., infected leaf, soil and seeds which assumed to have mycelium. The result indicate that FTA Card is effective as a collecting media of pathogen for most samples, paticularly for mycelium culture. Furthur experiments are still required to ensure the suitability of the card for leaf, seeds and soil samples.
HUBUNGAN KEKERABATAN ANTAR POPULASI JATI (Tectona Grandis,Linn.F.) BERDASARKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) AYPBC Widyatmoko; Anto Rimbawanto; Abdul Razaq Chasani
Jurnal Pemuliaan Tanaman Hutan Vol 7, No 3 (2013): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2013.7.3.151-166

Abstract

Information on genetic relationship amongst teak (Tectona grandis Linn.f) populations in Indonesia is important, in order to maintain the high genetic diversity of the species and to ensure the origin of commercial planting materials. In this study, 94 loci from 25 RAPD primers were used for analyzing genetic diversity and genetic relationship among 30 populations of teak which collected from provenance trial in Bojonegoro and its populations in Sulawesi. Mean genetic diversity (h) of the 30 populations was 0.184, and mean genetic distance between populations (D) was 0.441. Based on cluster analysis, 30 populations of teak were divided into 3 groups. The first group consisted of Burma, the second group consisted of Jawa, India, Indochina and Thailand populations, and the third group consisted of all  populations in Sulawesi. High genetic distance between Jawa’s and Sulawesi’s populations can be used to  differentiate seed/seedling from both regions.
ISOLASI DAN IDENTIFIKASI SENYAWA ANTIFUNGAL AKAR Acacia mangium DAN AKTIVITASNYA TERHADAP Ganoderma lucidum Nur Hidayati; SM Widyastuti; Subagus Wahyuono
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.1.11-26

Abstract

Acacia mangium has been planted on large scale of industrial forest plantation in Indonesia, especially in Sumatera and Kalimantan islands. It has been reported that huge number of mangium plantations on those areas infected rot root disease caused by Ganoderma lucidum. To date, there was no information of mangium which resist to Ganoderma lucidum Moreover, research to get this information had been carried out with two aims as listed below: (1) isolate and identify a compound with antifungal properties from the roots of healthy mangium, and (2) identify the effect of the antifungal compound from roots of healthy mangium on Ganoderma lucidum The roots of healthy mangium from the first generation of seedling seed orchard in Wonogiri, Central Java, were used as material of this research. Mangium roots which had had their external and internal parts separated were macerated in a solvent of n-hexane and methanol. Methods of the isolation of the antifungal compound were the thinlayer chromatography (TLC), column  chromatography and thin layer preparative chromatography. Antifungal effect test was carried out by using inhibition of germination and of hyphal growth of Fusarium sp. Ultraviolet (UV) spectrometry, Infrared (IR) and Gas Chromatography – Mass Spectrometry (GC-MS) were used to identify the antifungal compound. Antifungal effect test on Ganoderma lucidum was done with a modification of the cylinder plate method, performed in vitro.The results revealed that the antifungal compound succeed isolated in its Substance B form from methanol extract from the interior of the root. Substance B showed the highest level of antifungal activity through inhibiting germination and inhibiting of germination tube growth of Fusarium sp. This was shown by the highest percentage inhibiting of germination (66,67%), and the highest percentage inhibiting of germination tube (66,03%). The inhibition zone of hyphal growth of Ganoderma lucidum macroscopically from the antifungal compound was observed at a concentration of 1800 μg/ml. Microscopically, in the area of contact with the antifungal compound, hyphal curling and distorting of tips took place at a concentration of 1500 μg/ml one day after application of the antifungal compound. Based on the analysis of GC-MS spectra, the antifungal was identified as  p-Methoxybenzylidene p aminophenol in the category of phenolic compounds.

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