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Pendidikan Biologi
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PENGARUH pH TERHADAP AKTIVITAS PENISILIN G ASILASE YANG DIHASILKAN ISOLAT BAKTERI DARI AIR BATANG KURAO KOTA PADANG Desriyati, Desriyati; Fifendy, Mades; Siregar, RRP Megahati
Pendidikan Biologi Vol 2, No 1 (2013)
Publisher : STKIP PGRI Sumbar

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Abstract

Many pathogenic microorganisme were found to be resistant to penicillin, to prevent the occurrence of penicillin resistance is then produced semisynthetic penicillins, basic materials used for the manufacture of semisynthetic penicillins a 6-aminopenisilanat acid (6-APA). 6-APA is the result of hydrolysis of penicillin by the enzyme activity of penicillin G acylase, Penicillin G acylase produced by various microorganisme, such as fungi and bacteria. This study aimed to determine the effect of pH on the activity of penicillin G acylase resulting bacterial isolates from stem water Kurao Padang. This study was conducted from October 2012 to January 2013 in the Laboratory Kopertis X Padang region of West Sumatra. This type of research is that the experimental treatment pH variation between (6,5 - 8,0) of the PGA activity resulting bacterial isolates and calculate the resulting enzyme activity using the method of Kornfeld is a method that has a high sensitivity and fast in detecting compounds 6-aminopenisilanat acid (6-APA). Results showed pH affects the activity of PGA by bacterial isolates from water with pH variation stems Kurao PGA 0.0101 6.5 activity units / ml, at pH 7.0 activity PGA 0.0269 units / ml, where as at pH 7.5 PGA activity of 0.0200 units / ml, and the pH 8.0 activity PGA 0.0064 units / ml. Based on the research that has been done can be concluded that the activity of Penicillin G Acylase resulting bacterial isolates from water Kurao Batang Padang has an optimum pH of 7.0 with enzyme activity (AE) PGA 0.0269 units / ml.
PENGARUH pH TERHADAP AKTIVITAS PENISILIN G ASILASE YANG DIHASILKAN ISOLAT BAKTERI DARI AIR BATANG KURAO KOTA PADANG Desriyati, Desriyati; Fifendy, Mades; Megahati Siregar, RRP
Pendidikan Biologi Vol 2, No 1 (2013)
Publisher : STKIP PGRI Sumbar

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Abstract

Many pathogenic microorganisme were found to be resistant to penicillin, to prevent the occurrence of penicillin resistance is then produced semisynthetic penicillins, basic materials used for the manufacture of semisynthetic penicillins a 6-aminopenisilanat acid (6-APA). 6-APA is the result of hydrolysis of penicillin by the enzyme activity of penicillin G acylase, Penicillin G acylase produced by various microorganisme, such as fungi and bacteria. This study aimed to determine the effect of pH on the activity of penicillin G acylase resulting bacterial isolates from stem water Kurao Padang. This study was conducted from October 2012 to January 2013 in the Laboratory Kopertis X Padang region of West Sumatra. This type of research is that the experimental treatment pH variation between (6,5 - 8,0) of the PGA activity resulting bacterial isolates and calculate the resulting enzyme activity using the method of Kornfeld is a method that has a high sensitivity and fast in detecting compounds 6-aminopenisilanat acid (6-APA). Results showed pH affects the activity of PGA by bacterial isolates from water with pH variation stems Kurao PGA 0.0101 6.5 activity units / ml, at pH 7.0 activity PGA 0.0269 units / ml, where as at pH 7.5 PGA activity of 0.0200 units / ml, and the pH 8.0 activity PGA 0.0064 units / ml. Based on the research that has been done can be concluded that the activity of Penicillin G Acylase resulting bacterial isolates from water Kurao Batang Padang has an optimum pH of 7.0 with enzyme activity (AE) PGA 0.0269 units / ml.
PENGARUH pH TERHADAP AKTIVITAS PENISILIN G ASILASE OLEH ISOLAT BAKTERI DARI AIR BATANG ARU LUBUK BEGALUNG KOTA PADANG Ningsih, Dini Yusta Prestu; Fifendy, Mades; Megahati Siregar, RRP
Pendidikan Biologi Vol 2, No 1 (2013)
Publisher : STKIP PGRI Sumbar

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Abstract

Natural Penicillins (penicillin G) is known as an antibiotic which is chemically unstable, such as the onset of allergic in the body and its narrow spectrum. We perform the repairproperties of penicillin that makes penicillin G derivatives, in order to obtain a semisyntheticpenicillin that are better. Development of new penicillin can be reached by modifying thechemical spectrum penicillin G into 6-APA which is at the core of penicillin. To hydrolyzepenicillin G into 6-APA required the PGA enzyme produced by bacteria. PGA is anequilibrium reaction activity is influenced by pH. Under these conditions, has done research onthe effect of pH on PGA activity by bacterial isolates from Air batang Aru Lubuk BegalungPadang. The purpose of this study was to determine the effect of pH on PGA activity bybacterial isolates from Air Batang Aru. The research was conducted in the laboratory KopertisX from October 2012 to January 2013. This study is an experimental study with the method ofKornfeld on variation between pH (6.5, 7.0, 7.5, and 8.0). These results indicate that the pHeffect on the activity of PGA at pH 6.5 variation (0.0076 U / ml), pH 7.0, (0.0114 U / ml), pH7.5 (0.0609 U / ml ), and pH 8.0 (0.0200 U / ml). PGA optimum activity produced by bacteriaisolates were at pH 7.5
KEPADATAN POPULASI KUTU DAUN (Myzus persicae) DAN PREDATORNYA (Monoshillus sexmaculata) PADA TANAMAN CABE (Capsicum annum) DI KECAMATAN KOTOPARIK GADANG DIATEH KABUPATEN SOLOK SELATAN Dafrinal, Dafrinal; Widiana, Rina; Lusi, Armein
Pendidikan Biologi Vol 2, No 1 (2013)
Publisher : STKIP PGRI Sumbar

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Abstract

Chili is a herbaceous plant of the family eggplant (Solanaceae) which has the scientificname Capsicum annuum L. Chili has a lot of nutrients and vitamins, such as calories, protein, fat,carbohydrates, calcium, Vitamin A, Vitamin B1 and Vitamin C. Besides being used for domesticpurposes, peppers can also be used for industrial purposes such as, industry-seasoning, food andmedicine or herbal. Efforts to increase the production of chili still have problems due to pestaphids (Myzus persicae). Aphids reproduce in two ways: with and without marriage or their eggscan develop into young without fertilization (Partegonesis). This pests life cycle ranges from 7-10 days. These pests attack plants by sucking liquid chili leaves, shoots, flower stalks or otherplant parts, so that the leaves become mottled yellow (chlorotic) and eventually fall so chiliproduction declined. This insect will be clustered so as to cover part of the plant. Of control thispest than by using insecticides as well as biological control by predators. Predator beetles arefound is the javelin (Menochillus sexmaculata). Has done research on leaf Mite PopulationDensity (Myzus persicae) and the predatory beetle javelin (Menochillus sexmaculata) At Chilliplants. This study was conducted using a descriptive survey in April 2012, from the results, thepopulation density of aphids Myzus persicae with an average of 64.4 people per stick plantpredatory beetles and javelin Menochillus sexmaculata with an average of 2.4 individuals perstick plant.
ANA LISIS ISI LAMBUNG PENSI (Corbicula moltkiana) YANG TERDAPAT DI DANAU MANINJAU Desyiska, Desyiska; Nurhadi, Nurhadi; Abizar, Abizar
Pendidikan Biologi Vol 2, No 1 (2013)
Publisher : STKIP PGRI Sumbar

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Abstract

Maninjau Lake is one lake in West Sumatra and ample potential. Maninjau currently used for various activities, such as fisheries, tourism, Hydroelectric Power Plant (HEPP), Shower Wash latrine (toilet) and disposal of household waste. The last few years the population activity in the body and on the edge of the lake has increased as fish farming system with floating nets. Activities around the lake residents and lake bodies, suspected to cause impaired. Her balance of the lake ecosystem which affect lake biota. such as art shows moltkiana Corbicula. The presence of Corbicula pensi moltkiana in Maninjau can be used as an additional income for the communities around the lake. But according to the day pensi harvester Corbicula moltkiana hard to come by. In connection with that the authors have conducted a study with the aim of knowing the natural food of pensi. The study was conducted in May 2012 with a descriptive survey method by setting the four stations based activities alongside a lake and the lake body Muko-Muko as a tourism spot (Station I), Forage Fish farming cages Rabaa (Station II), Bayur Agricultural area (Station III ), and community activities Maninjau quite high (station IV). In this research pensi pensi used is larger than 15mm. On each - each station established 3 sampling points. Pensi samples taken 6 tails of each station. At each sampling point taken 2 pensi tail. Laboratory sample identification STKIP PGRI West Sumatra. Parameters measured were relative density and relative frequency as well as water quality parameters measured were temperature and pH. From the research found 14 types of natural foods pensi belonging to class
PENGARUH EKSTRAK MARKISA (Passiflora edulis varietas flavicarpa) TERHADAP FEEDING STRATEGY LALAT BUAH Bactrocera dorsalis He nd. Oktavia, Delni; Sumarmin, Ramadhan; Lusi Zeswita, Armein
Pendidikan Biologi Vol 2, No 1 (2013)
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Marquisa is one of favourite fruit in Indonesia. However this plant is oftenattacked by diseases, one of them is Bactrocera dorsalis. There is no information yetabout what interest Bactrocera dorsalis to marquisa does. Based on the problem, theaim of the research is effect of marquisa extrac t (Passiflora edulis varietas flavicarpa)toward Feeding Strategy of fruit fly Bactrocera dorsalis Hend. T his research wasexperimental research used cilinder Y tube method, with match treatment and includes10 repetitions. The research was conducted from October 2012 to February 2013. Theresult, show that marquisa extract have effect toward Feeding Strategy from fruit flyBactrocera dorsalis. On the searching activity, the fastest time of fruit fly reachedmarquisa was at the treatment V (75,13 seconds) although longest time was at treatmentI (256,33 seconds). On the grounding ac tivity, the fastest time of fruit fly reachedmarquisa was at the treatment V (45,23 seconds) although the longest time of treatmentI (58,7 seconds). Based on Chi-square analysis the treatment had the higher differencebetween petrogenol and marquisa extract, excepting treatment 3 ml of marquisa extractand 3 drops of petrogenol did not have real difference between petrogenol and marquisaextrac t. It cand conclude that marquisa extract had effect toward Feeding Strategy offruit fly Bactrocera dorsa lis.
KEANEKARAGAMAN ALGA EPILITIK DI SUMBER AIR PANAS BUKIK GADANG KECAMATAN LEMBANG JAYA KABUPATEN SOLOK Anggraini, Devi; Risdawati, Renny; Nursyahra, Nursyahra
Pendidikan Biologi Vol 2, No 1 (2013)
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Epilitic algae is part of a group microperifitic algae that lives attached to the substrate (stone,coral, gravel and other hard objects). Solok Regency has Talang Mount with the beautifulnatural that can be enjoyed, this place also has a hot water source in Bukik Gadang, NagariKoto Anau, Lembang Jaya District, Solok Regency which is believed has a power to heal ortreat and as a tourism object. The temperature of hot water is about 39 – 45 ºC. The researchof epilitic algae in Bukik Gadang hot spring, Lembang Jaya District, Solok Regency in haddone Desember 2012 – Maret 2013. The purpose of this research to know the variety ofepilitic algae at different temperatures. This research has done by using a descriptive surveymethod with determining three station based on the different temperatures and sampling wasdone by purposive sampling at each station were taken three points. This research found 17species of epilitic algae consist of two classes of Bacillariophyceae and Cyanophyceae. Theclass Bacillariophyceae found 9 species and class Cyanophyceae 8 species that live attemperatures of 39 – 45 ºC, pH 7,01 – 7,50, dissolved oxygen 1,44 – 3,96 mg/l, the sulfurcontent from 0,089 – 0,105 mg/l.
KEPADATAN POPULASI KUTU DAUN (Myzus persicae) DAN PREDATORNYA (Monoshillus sexmaculata) PADA TANAMAN CABE (Capsicum annum) DI KECAMATAN KOTOPARIK GADANG DIATEH KABUPATEN SOLOK SELATAN Dafrinal, Dafrinal; Widiana, Rina; Lusi, Armein
Pendidikan Biologi Vol 2, No 1 (2013)
Publisher : STKIP PGRI Sumbar

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Abstract

Chili is a herbaceous plant of the family eggplant Solanaceae) which has the scientific name Capsicum annuum L. Chili has a lot of nutrients and vitamins, such as calories, protein, fat, carbohydrates, calcium, Vitamin A, Vitamin B1 and Vitamin C. Besides being used for domestic purposes, peppers can also be used for industrial purposes such as, industry-seasoning, food and medicine or herbal. Efforts to increase the production of chili still have problems due to pest aphids (Myzus persicae). Aphids reproduce in two ways: with and without marriage or their eggs can develop into young without fertilization (Partegonesis). This pests life cycle ranges from 7-10 days. These pests attack plants by sucking liquid chili leaves, shoots, flower stalks or other plant parts, so that the leaves become mottled yellow (chlorotic) and eventually fall so chili production declined. This insect will be clustered so as to cover part of the plant. Of control this pest than by using insecticides as well as biological control by predators. Predator beetles are found is the javelin (Menochillus sexmaculata). Has done research on leaf Mite Population Density (Myzus persicae) and the predatory beetle javelin (Menochillus sexmaculata) At Chilli plants. This study was conducted using a descriptive survey in April 2012, from the results, thepopulation density of aphids Myzus persicae with an average of 64.4 people per stick plant predatory beetles and javelin Menochillus sexmaculata with an average of 2.4 individuals per stick plant.
PENGARUH ANTIFEEDANT EKSTRAK KULIT BATANG ANGSANA (Pterocarpus indicus Willd.) TERHADAP FEEDING STRATEGY WERENG COKLAT (Nilaparvata lugens Stal.) Anggriani, Desi; Sumarmin, Ramadhan; Widiana, Rina
Pendidikan Biologi Vol 2, No 1 (2013)
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The Nilaparvata lugens Stal. is a harming parasitoid crop. It’s difficult to eliminatebecause it have faster metamorphosis . This parasitoid can form blooming population in ashort time and destroy crop at all of growth phase. The aim of this research is to know theeffects of Antifeedant extrac of Pterocarpus indicus Willd. to Feeding Strategy Nilaparvatalugens Stal. This research used Completely Randomized Design ( CRD) with 4 treatment and10 replication. The treatment are concentration 0 %, 2.5 %, 5 % and 7.5 %. The data analysisby ANOVA and than continued with LSD. This experiment conducted in ZoologyLaboratory of FMIPA UNP at February 2013. Result shows that concentration 5 % activitysearching Nilaparvata lugens Stal. require time which fastest to other treatment, the activityof grounding treatment with concentration 2.5 % requiring time which quickly while atactivity of gathering treatment with concentration 7.5 % very effective to pursue to eat it.Can conclude that extract of angsana at concentration 5 % can be use antifeedant byNilaparvata lugens Stal.
FREKUENSI SOIL TRANSMITTED HELMINTHS PADA MURID SEKOLAH DASAR NEGERI 31 PASIR KANDANG KECAMATAN KOTO TANGAH KOTA PADANG Rahmadani, Diana; Indriati, Gustina; Lusi Zeswita, Amein
Pendidikan Biologi Vol 2, No 1 (2013)
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Intestinal helminth infections are transmitted via soil (Soil Transmitted Helminths) isstill a health problem facing society in Indonesia. The STH worms infect more children livingin slums and have poor sanitation. Elementary School is located at 31 Pasir Kandang lowlandcoastal areas that the average population has lower economic level and have inadequate toiletfacilities. Therefore research has been carried out with the aim to determine the frequency ofSoil Transmitted Helminths in Primary School Pupils Pasir Kandang 31 Sub Tangah KotoPadang. December 9, 2012 using descriptive methods.The study was conducted onDecember 2 The population in this study were students of SDN 31 Pasir Kandang as manyas 368 people. Sampling was done by random sampling of a population of 57 people. Stoolexamination conducted at the Laboratory of Biology Education Program STKIP PGRI WestSumatera by way of direct observation. The results showed that students of SDN 31 STHinfection as much as 59.65% with the percentage of each species from infected (20.59%Ascaris lumbricoides, Trichuris trichiura 14.71%, 5.88% hookworm, Ascaris lumbricoidesand Trichuris trichiura 35.29%, Trichuris trichiura and hookworm 2.94% after Ascarislumbricoides, Trichuris trichiura and hookworm 20.59%).

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