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PENGARUH pH TERHADAP AKTIVITAS PENISILIN G ASILASE YANG DIHASILKAN ISOLAT BAKTERI DARI AIR BATANG KURAO KOTA PADANG Desriyati, Desriyati; Fifendy, Mades; Siregar, RRP Megahati
Pendidikan Biologi Vol 2, No 1 (2013)
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Many pathogenic microorganisme were found to be resistant to penicillin, to prevent the occurrence of penicillin resistance is then produced semisynthetic penicillins, basic materials used for the manufacture of semisynthetic penicillins a 6-aminopenisilanat acid (6-APA). 6-APA is the result of hydrolysis of penicillin by the enzyme activity of penicillin G acylase, Penicillin G acylase produced by various microorganisme, such as fungi and bacteria. This study aimed to determine the effect of pH on the activity of penicillin G acylase resulting bacterial isolates from stem water Kurao Padang. This study was conducted from October 2012 to January 2013 in the Laboratory Kopertis X Padang region of West Sumatra. This type of research is that the experimental treatment pH variation between (6,5 - 8,0) of the PGA activity resulting bacterial isolates and calculate the resulting enzyme activity using the method of Kornfeld is a method that has a high sensitivity and fast in detecting compounds 6-aminopenisilanat acid (6-APA). Results showed pH affects the activity of PGA by bacterial isolates from water with pH variation stems Kurao PGA 0.0101 6.5 activity units / ml, at pH 7.0 activity PGA 0.0269 units / ml, where as at pH 7.5 PGA activity of 0.0200 units / ml, and the pH 8.0 activity PGA 0.0064 units / ml. Based on the research that has been done can be concluded that the activity of Penicillin G Acylase resulting bacterial isolates from water Kurao Batang Padang has an optimum pH of 7.0 with enzyme activity (AE) PGA 0.0269 units / ml.
`KOMPOSISI RAYAP DI KEBUN GAMBIR DI KANAGARIAN SIGUNTUR MUDA KECAMATAN KOTO XI TARUSAN KABUPATEN PESISIR SELATAN Aidi, Rido; Nurhadi, Nurhadi; Safitri, Elza
Pendidikan Biologi Vol 2, No 2 (2013): Jurnal Mahasiswa Pendidikan Biologi Genap 2013-2014
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AbstractGrowth of human being activity to fulfill requirement of its life oftentimes change role of termite. Environmental which do not annoyed termite have role in process of decomposition of organic garbage. Change of forest areal become agriculture areal, plantation oftentimes change role of termite become pest. In Kanagarian Siguntur in general society represent farmer of gambir, so that most forest processed to become garden of gambir, which can disturb habitat of termite ( Isoptera). Hence have been done research of concerning Composition Termite at Garden of Gambir in Kanagarian Siguntur Muda, District Of Koto XI Tarusan Sub-Province Pesisir Selatan. This Research is done to know termite composition ( Isoptera) in garden of gambir. This research is done in Agustus-September 2013, with used method of hand sort with two station. Station I is garden of gambir and station II is forest around garden of gambir. Intake sample of termite is done broadly used plot 50 X 50 cm, that consist of 3 substasiun. Apart between substasiun 20 m. Each substasiun consist of 5 plot which is the each aparting 10 m. Pursuant to result of research which have been done by composition termite in garden of gambir found as many as two family which consist of three subfamili, three species, and 639 individual. Meanwhile at forest, found composition termite as many as the two family which consist of four subfamili, four species, and 875 individual. chemical Physics factor of temperature ground, ground water rate, ground pH, and rate of C organic of ground according to support life of termite.
PENGARUH pH TERHADAP AKTIVITAS PENISILIN G ASILASE YANG DIHASILKAN ISOLAT BAKTERI DARI AIR BATANG KURAO KOTA PADANG Desriyati, Desriyati; Fifendy, Mades; Megahati Siregar, RRP
Pendidikan Biologi Vol 2, No 1 (2013)
Publisher : STKIP PGRI Sumbar

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Abstract

Many pathogenic microorganisme were found to be resistant to penicillin, to prevent the occurrence of penicillin resistance is then produced semisynthetic penicillins, basic materials used for the manufacture of semisynthetic penicillins a 6-aminopenisilanat acid (6-APA). 6-APA is the result of hydrolysis of penicillin by the enzyme activity of penicillin G acylase, Penicillin G acylase produced by various microorganisme, such as fungi and bacteria. This study aimed to determine the effect of pH on the activity of penicillin G acylase resulting bacterial isolates from stem water Kurao Padang. This study was conducted from October 2012 to January 2013 in the Laboratory Kopertis X Padang region of West Sumatra. This type of research is that the experimental treatment pH variation between (6,5 - 8,0) of the PGA activity resulting bacterial isolates and calculate the resulting enzyme activity using the method of Kornfeld is a method that has a high sensitivity and fast in detecting compounds 6-aminopenisilanat acid (6-APA). Results showed pH affects the activity of PGA by bacterial isolates from water with pH variation stems Kurao PGA 0.0101 6.5 activity units / ml, at pH 7.0 activity PGA 0.0269 units / ml, where as at pH 7.5 PGA activity of 0.0200 units / ml, and the pH 8.0 activity PGA 0.0064 units / ml. Based on the research that has been done can be concluded that the activity of Penicillin G Acylase resulting bacterial isolates from water Kurao Batang Padang has an optimum pH of 7.0 with enzyme activity (AE) PGA 0.0269 units / ml.
PENGARUH PENERAPAN MODEL PEMBELAJARAN KOOPERATIF TIPE ROUND ROBIN TERHADAP HASIL BELAJAR BIOLOGI SISWA KELAS XI IPA SMAN 2 BAYANG KABUPATEN PESISIR SELATAN Wahyuni, Yola Putri; Mulyati, Mulyati; Yasmi, Vivi
Pendidikan Biologi Vol 2, No 2 (2013): Jurnal Mahasiswa Pendidikan Biologi Genap 2013-2014
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ABSTRACTThe problem in this research is the result of learning biology for students class XI Science SMAN 2 Bayang is still low. The low this learning outcome was due to low student interest towards the subjects of biology, students are still less active as a teacher-centered learning, to overcome these problems, one solution is the application of cooperative learning model Round Robin with a model where the students demanded more active. This study was conducted to determine whether students learning outcomes biology class XI Science SMAN 2 Bayang with cooperative learning model Round Robin is better than the results of biological studies students with conventional learning. This type of research is experimental, with a design that used Randomize Control Only Design. Population in this research is class XI Science SMAN 2 Bayang . Sampling was conducted using purposive sampling technique. Experimental class is class XI IPA1 and control class is class XI IPA2. The instrument used in this study is the final test. Form of objective tests used with option 5. Reliability test is R11 = 0.94. The data analysis technique used is the one party  test. Based on the analysis of the final test results obtained thit = 2,39 and the table = 1,645. so we can conclude the results of biological studies students with cooperative learning model Round robin is better than the results of biological studies with conventional learning students in class XI Science SMAN 2 Imagine the Academic Year 2013/2014 . This means that cooperative learning model Round Robin has a positive effect on learning outcomes. 
PENGARUH pH TERHADAP AKTIVITAS PENISILIN G ASILASE OLEH ISOLAT BAKTERI DARI AIR BATANG ARU LUBUK BEGALUNG KOTA PADANG Ningsih, Dini Yusta Prestu; Fifendy, Mades; Megahati Siregar, RRP
Pendidikan Biologi Vol 2, No 1 (2013)
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Natural Penicillins (penicillin G) is known as an antibiotic which is chemically unstable, such as the onset of allergic in the body and its narrow spectrum. We perform the repairproperties of penicillin that makes penicillin G derivatives, in order to obtain a semisyntheticpenicillin that are better. Development of new penicillin can be reached by modifying thechemical spectrum penicillin G into 6-APA which is at the core of penicillin. To hydrolyzepenicillin G into 6-APA required the PGA enzyme produced by bacteria. PGA is anequilibrium reaction activity is influenced by pH. Under these conditions, has done research onthe effect of pH on PGA activity by bacterial isolates from Air batang Aru Lubuk BegalungPadang. The purpose of this study was to determine the effect of pH on PGA activity bybacterial isolates from Air Batang Aru. The research was conducted in the laboratory KopertisX from October 2012 to January 2013. This study is an experimental study with the method ofKornfeld on variation between pH (6.5, 7.0, 7.5, and 8.0). These results indicate that the pHeffect on the activity of PGA at pH 6.5 variation (0.0076 U / ml), pH 7.0, (0.0114 U / ml), pH7.5 (0.0609 U / ml ), and pH 8.0 (0.0200 U / ml). PGA optimum activity produced by bacteriaisolates were at pH 7.5
TINGKAT KEMATANGAN GONAD KEPITING BAKAU Scylla paramamosain Estampador DI HUTAN MANGROVE TELUK BUO KECAMATAN BUNGUS TELUK KABUNG KOTA PADANG Samsu, Fetro Dola; Sumarmin, Ramadhan; Zeswita, Armein Lusi
Pendidikan Biologi Vol 2, No 2 (2013): Jurnal Mahasiswa Pendidikan Biologi Genap 2013-2014
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Abstract:Scylla paramamosain E. is one type of crab that lives in mud substrat at mangroveareas Buo Gulf. Scylla Paramamosain E. is favored by the people in the Buo Gulf. Now Scylla paramamosain E. population has begun to decrease and sizes caught are relatively small . Based on at this research take plan to identification gonad maturity of Scylla paramamosain E. at mangrove forest in the Buo Gulf Bungus of Padang. This study was conducted in May until July 2013 used the descriptive method. The results showed there were different gonad maturity of Scylla paramamosain was caught in the Buo Gulf. In May, Scylla paramamosain E. had immature gonads 33.33 % while the percentage of matured at a rate of66.67 % . In June, the percentage of immature gonads and matured amount 30.0% and 70 % . In July, there were 41 , 66 % in the immature stage and 58.32 % matured. Among of the three months, gonad maturity Scylla paramamosain E in July at the mostly mature gonad condition and ready for spawning. In addition biogeochemical cycles can influence and bring sufficient nutrients for survival Scylla paramamosain E. In general the level gonad maturity in Scylla paramamosain E. in the Gulf Buo mangrove forest in May to July 2013 TKG I5.68 % , 29.32 % TKG II , III TKG 21.27 % , 11.15 % IV TKG , TKG V 32.59 %. Environmental factors such as temperature measured during the study ranged from 280C , salinity ranges from 31 % o - 32 % o , pH around 7 ( Neutral ) and an average water depth of 58.5 cm .    
PENGARUH MEDIA TANAM TERHADAP PERTUMBUHAN VEGETATIF TANAMAN MENTIMUN (Cucumis sativus L.) Arisandi, Nirmela; Mulyati, Mulyati; Novi, Novi
Pendidikan Biologi Vol 2, No 2 (2013): Jurnal Mahasiswa Pendidikan Biologi Genap 2013-2014
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ABSTRAK Cucumber is an annual plant that is pervasive belonging to Cucurbitaceae family are widely used as food , cosmetic and drug industry - abatan . Low productivity and quality of cucumber caused less intensive farming techniques , one of which depends on fertilization and treatments performed . The initial step was  to  produce  healthy  seedlings  with  optimal  growth  is  to  find  a  suitable growing media for planting cucumbers . To find the appropriate planting medium with the plant roots need to combine the organic material and adapted to the type of plant . In connection with that have done research that aims to find a suitable planting medium for the growth of cucumber plants . The research was conducted in the village Kajai , Talamau District , West Pasaman . This type of research is experimental , with a completely randomized design ( CRD ) consisting of 5 treatments and 5 replications . The treatments were A ( soil as a control ) , B ( garden soil + sand ) , C ( garden soil + manure ) , D ( cow manure + sand ) , E ( garden soil + sand + cow manure ) . The data were analyzed with analysis of variance and followed by LSD test at 5% level . The results showed that the use of a combination  of  growing media  can  increase  the percentage  of  live  seeds  , number of leaves and plant height . The best growth results found on the treatment E.  
TINGKAT KEMATANGAN GONAD KEPITING RAJUNGAN (Portunus pelagicus L.) DI HUTAN MANGROVE TELUK BUO KECAMATAN BUNGUS TELUK KABUNG KOTA PADANG Nika, Restia; Sumarmin, Ramadhan; Zeswita, Armein Lusi
Pendidikan Biologi Vol 2, No 2 (2013): Jurnal Mahasiswa Pendidikan Biologi Genap 2013-2014
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ABSTRACTGonad maturity is as describes the ability of reproduction of aquatic animals including Blue sweemer crab. Rajungan crab Portunus pelagicus L. requires Mangrove area as a place of ( Nursery grounds and Feeding grounds. This study was conducted  to determine the of gonad maturity of Rajungan crab Portunus pelagicus L. in Buo Gulf Mangrove, the Bungus Teluk Kabung, Padang. The research was conducted from May to July 2013 using the descriptive method through observation of gonadal morphology of Rajungan crab Portunus pelagicus L. Data analisis of  Gonad Maturity using the percentage. The result showed that the female Rajungan crab is 33. Gonad Maturity percentage in May - July  Gonad Maturity I : 39.39%, Gonad Maturity II 27.27%, Gonad Maturity III 12.12%, Gonad Maturity IV 12.12%, Gonad Maturity V 9.09%. Observations on physicl-chemical water parameters during three months ware observed that ranged from 28.40 - 30.80C0, salinity from 32.3 to 34.5 ppm, pH 7 - 8, and the depth  65cm - 3m. 
PENGARUH PEMBERIAN PAKAN ORGANIK NT45 SERI I TERHADAP PERTUMBUHAN BIBIT IKAN PATIN JAMBAL (Pangasius djambal) Rizki, Zaidan; Razak, Abdul; Widiana, Rina
Pendidikan Biologi Vol 2, No 2 (2013): Jurnal Mahasiswa Pendidikan Biologi Genap 2013-2014
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ABSTRACT Freshwater fish farming has become an indispensable agribusiness activities with fishmeal industry, one of which Jambal Catfish ( Pangasius djambal ). Feed the fish that have been used so far by ranchers/farmers, known as fish pellets. Feed pellet mill is easy to come by, but the feed pellets can damage the environment, because it can lead to water pollution. Additionally pellet prices continue to rise each year. In contrast to the NT45 Organic food contains bacteria that can clean water penggurai of turbidity and can fertilize with water triggers the Planton. Organic feed NT45 Series I also do not use synthetic chemicals and does not contain toxins and the price is also cheaper. Based on the background above, has done research on The Effect of Organic Feed NT45 Series I on growth of seedlings Jambal Catfish (Pangasius djambal). The purpose of this study was to observe the effect of feeding organic NT45 Series I on the growth of seedlings jambal catfish (Pangasius djambal). This study was conducted in February and March, 2013, housed in the Department of Biology FIMPA Ecology Laboratory, State University of West Sumatra Padang. This study is an experimental study. This study was designed with RAL (completely randomized design) with 4 treatments and 6 replications. The sample in this study is Jambal Catfish (Pangasius djambal) aged 8 weeks. The parameters measured were the length of the total, the length and weight of fish standards. Data analysis was performed with Variety Analysis and Test followed by Least Significant Difference (LSD) at α 5 %. The results showed that the organic feed NT45 Series I have a positive influence on the growth of seedlings Jambal Catfish (Pangasius djambal) both the length of the fishs body and the body weight gain Jambal Patin. Organic feed number NT45 Series I best to improve weight and body length Catfish Jambal is 3.78 g/day. 
UJI BAKTERIOLOGIS CABAI MERAH GILING (Capsicum annum L.) DARI BEBERAPA PASAR TRADISIONAL DI KOTA PADANG Musliati, Desi; Fifendy, Mades; Periadnadi, Periadnadi
Pendidikan Biologi Vol 2, No 2 (2013): Jurnal Mahasiswa Pendidikan Biologi Genap 2013-2014
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Abstract Chilli is one of commodity that never left by people. As the development of technology , instant production greatly favored by the people. One of that is minced chili that made from red chilli. From the market survey to minced red chilli tradisional traders at traditional market of Padang is that red chilli processing into minced chilli for public consumption still does not fulfill the requirements in terms of hygiene.This study aimed to test the bacteriological to minced red chlli ( Capsicum annum L.) from some traditional market in Padang City, which was conducted in June2013  at  the  Microbiology  Laboratory of  the  Department  of  Biology,  Andalas  University.This research was conducted with descriptive methods. Sample taking was picked up by purposive sampling which 2 sample were taken from each 5 traditional market in Padang, consist of Bandar Buat market, Gaung market, Lubuk Buaya market, Simpang Haru market, and Siteba market.Bacteriological testing is then performed with the steps :1.estimation test ( presumtive test ),2 . confirmation test ( confirmed test ) and 3 . completion test (completed test ) using the MPN method (Most Probable Number) by using a combination of 5:1:1. From the research, results obtained that minced red chili sample contain bacteria with the range number 43 x        cell/ml - 157 x         cell/ml. Minced red chili sample from five traditional market in Padang city have coliform bacteria MPN values range from 12.0 to 240.0 ml sample, and E. Coli bacteria from 7.5 to 240.0 cells / 100 ml sample. Concluded that minced red chili that sold in some traditional market in Padang city is generally not suitable for consumption because it contains E. coli > 3 colonies / g and coliform bacteria > 100 colonies / g. 

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