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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 15 Documents
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Search results for , issue "Vol 9 No 1 (2021)" : 15 Documents clear
Identification of endophytic fungi in nutgrass (Cyperus rotundus L.) as solubilizing phosphate and indole-3-acetic acid producers Nur Kusmiyati; Septian Tri Wicaksono; Durrotul Maknuna
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.21216

Abstract

Low phosphate content in the soil causes insufficient plant needs. Meanwhile, endophytic fungi in nutgrass have great potential as a phosphate solvent and produce indole-3-acetic acid (IAA). Therefore, this study aims to determine the solubilizing phosphate and IAA production by the endophytic fungi of nutgrass and identify the isolates based on rDNA-ITS sequences. Endophytic fungi isolates were cultured in 10 ml of Pikovskaya broth media with Ca5(PO4)3 as the inorganic phosphate source. The PCR results were analyzed using 1.5% agarose gel electrophoresis followed by sequence analysis. The isolation and purification results showed five isolates coded URT1, URT2, URT3, URT4, and URT5, while the solubilizing phosphate levels ranged from 54.03-87.83 ppm, with the highest levels found in the URT4 isolate. Furthermore, the IAA levels ranged from 5.58-45.50 ppm, with the highest levels produced by the URT1 isolate. The molecular analysis with rDNA-ITS sequences showed that URT4 had 97.42% similarity to Aspergillus terreus species, while UTR1 had 100% similarity to Fusarium oxysporum species. Based on the results, the endophytic fungi of nutgrass from A. terreus and F. oxysporum species have great potential as biofertilizers due to the high solubilizing phosphate and IAA levels.
Antibacterial activity test on ethanol extract fraction of Kirinyuh (Chromolaena odorata L.) leaves for multi-drug resistant organisms bacteria Endang Sulistyarini Gultom; Tri Hartanti; Hasnaul Maritsa; Eko Prasetya
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.17067

Abstract

The resistance of pathogenic bacteria to antibiotics is increasing due to antibiotics with incorrect doses, wrong diagnostics, and the wrong target. Bacteria that have been resistant to several antibiotics are called multi-drug resistant organisms (MDRO) bacteria. Bacterial resistance to some antibiotics requires alternative herbal treatments, one of which is the Chromolaena odorata L. Research must therefore be conducted on the antibacterial activity of the ethanol extract fraction of C. odorata L. leaves for MDRO bacteria, such as Staphylococcus lugdunensis methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa extended-spectrum beta-lactamase (ESBL), and Klebsiella pneumoniae ESBL. This study aims to determine the antibacterial activity of the ethanol extract of Kirinyuh leaves (C. odorata L.) and the antibacterial activity of the fractionation against MDRO bacteria. Test results of kirinyuh leaf ethanol extract for S. lugdunensis MRSA, P. aeruginosa ESBL, and K. pneumoniae ESBL bacteria each resulted in an inhibition zone with an average diameter of 11.6 mm (strong), 11.5 mm (strong), and 11.13 mm (strong), respectively. Testing the antibacterial activity of the ethanol fraction against MDRO bacteria can show antibacterial activity against all tested bacteria, namely Fraction 5. The results of the antibacterial activity of fraction 5 against K. pneumoniae ESBL, P. aeruginosa ESBL, and S. lugdunensis MRSA bacteria with the formation of inhibition zones formed of 10.2 mm (strong), 8.8 mm (moderate), and 7.9 mm (moderate), respectively. The results of thin-layer chromatography showed that the secondary metabolites contained in the fifth fraction were terpenoids, steroids, and flavonoids.
Effect of Chlorella vulgaris extract on high sucrose diet-induced diabetes in Drosophila melanogaster Mohamad Agus Salim; Muhammad Subandi; Yeni Yuniarti
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.18108

Abstract

Microalgae Chlorella vulgaris is a microscopic green alga known to have potential as a source of food and medicine (functional food). However, the pharmacological capacity of these microalgae species against disease due to metabolic disorders is not understood. Therefore, this study aims to evaluate the effect of C. vulgaris extract on animal models of Drosophila melanogaster, which are fed with a high-sucrose diet (HSD). In this study, the wild-type Drosophila used for seven days of observation was divided into four groups. The first group was used as a control without treatment, the second was treated with HSD (30%), the third was treated with C. vulgaris extract (120 g/L), and the fourth group had double treatment (HSD and C. vulgaris extract). Meanwhile, the parameters observed included fecundity, hatchability, hemolymph glucose, and triglyceride levels. The results showed that the C. vulgaris extract was able to reduce the negative effects of administering HSD. In addition, the single and double treatment of C. vulgaris extract and HSD significantly decreased the levels of hemolymph glucose as well as triglycerides of Drosophila. At the same time, the fecundity parameters and hatchability significantly increased compared to Drosophila that received only HSD treatment. Based on these results, C. vulgaris extract the has potential to be used as an antihyperglycemic agent. However, further study is recommended to prove it.
Ex-situ conservation of the native orchid Coelogyne rochussenii de Vriese from the Bukit Rimbang and Baling Wildlife Reserve Areas Pebra Heriansyah; Gusti Marlina
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.21274

Abstract

The native orchid Coelogyne rochussenii de Vriese is critically endangered, hence, conservative measures are needed to prevent extinction. Meanwhile, in-situ conservation is constrained by time, resources, and costs. Therefore, this study aims to identify the most effective method for ex-situ conservation, especially with basal medium and activated charcoal, to determine the fastest germination. The completely randomized factorial design of four different basal media treatments including Knudson C, Vacin and Went, Murashige and Skoog, and Hyponex + vitamin medium were used. These treatments were combined with the application of activated charcoal at four different levels, with concentrations of 0, 1, 2, and 3 g/L-1, meanwhile, the parameters observed include germination day and percentage, as well as contamination percentage. The results showed that the conservation of Native orchid C. rochussenii de Vriese using various growing media and activated charcoal had a significant effect on the growth of the embryo culture. Based on the results, the Hyponex + vitamin medium with 3 g/L-1 accelerated germination days to 29.33, increased germination percentage to 92.06%, and reduced contamination to 0%.
ISSR based analysis of genetic variability of plantlets culture of pineapple (Ananas comosus L.) from Sipahutar, North Sumatera, Indonesia Fauziyah Harahap; Annisa Afiva; Miftahul Jannah; Eko Prasetya
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.17068

Abstract

Sipahutar pineapple is very popular in North Sumatra, because of the distinctive sweet taste and normal water content. Furthermore, it is large in size and has a yellow fruit skin color with greenish tips. However, the problem with Sipahutar pineapple production is the limited amount of good quality seeds. The utilization of in vitro culture techniques on pineapples allows the emergence of somaclonal variations, especially in plantlets that have undergone the subculture stage. This somaclonal variation is one of the problems in commercial seedling production, therefore, it is necessary to conduct an initial examination using the inter simple sequence repeat (ISSR) molecular marker. This study aimed to determine the genetic and primary stability of ISSR, which can be used in pineapple plants native to Sipahutar. The methods used include sample preparation, DNA isolation, primer optimization, ISSR primer amplification by PCR method, and electrophoresis. Furthermore, a total of 15 samples were amplified with six ISSR primers, and the data were analyzed by cluster method using the NTSYS-PC software. The final result was visualized in a dendrogram and analysis of diversity was conducted using GenAlex. The results showed that the level of genetic variability of the Sipahutar pineapple, which has undergone in vitro culture using six ISSR molecular markers was 76-97%. Meanwhile, the genetic variability level of the native to Sipahutar pineapple can be influenced by the long culture period and the use of N6-benzyladenine. The primers can be used to observe the genetic variability, except for ISSR 25 with a PIC value of 0.000.

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