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All Journal Biogenesis: Jurnal Ilmiah Biologi JURNAL AGROTEKNOLOGI Jurnal Ilmiah Pertanian Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Journal of Tropical Crop Science Paspalum: Jurnal Ilmiah Pertanian Agro Bali: Agricultural Journal Akta Agrosia Jurnal Agro Indragiri Bhakti Nagori (Jurnal Pengabdian kepada Masyarakat)
Pebra Heriansyah
Universitas Islam Kuantan Singingi, Teluk Kuantan. Jln. Gatot Subroto KM 7 Jake Telp : 082383267805 Teluk Kuantan, Riau
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Physics

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Ex-situ conservation of the native orchid Coelogyne rochussenii de Vriese from the Bukit Rimbang and Baling Wildlife Reserve Areas Pebra Heriansyah; Gusti Marlina
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.21274

Abstract

The native orchid Coelogyne rochussenii de Vriese is critically endangered, hence, conservative measures are needed to prevent extinction. Meanwhile, in-situ conservation is constrained by time, resources, and costs. Therefore, this study aims to identify the most effective method for ex-situ conservation, especially with basal medium and activated charcoal, to determine the fastest germination. The completely randomized factorial design of four different basal media treatments including Knudson C, Vacin and Went, Murashige and Skoog, and Hyponex + vitamin medium were used. These treatments were combined with the application of activated charcoal at four different levels, with concentrations of 0, 1, 2, and 3 g/L-1, meanwhile, the parameters observed include germination day and percentage, as well as contamination percentage. The results showed that the conservation of Native orchid C. rochussenii de Vriese using various growing media and activated charcoal had a significant effect on the growth of the embryo culture. Based on the results, the Hyponex + vitamin medium with 3 g/L-1 accelerated germination days to 29.33, increased germination percentage to 92.06%, and reduced contamination to 0%.
PENGARUH PEMBERIAN MYOINOSITOL DAN ARANG AKTIF PADA MEDIA SUB KULTUR JARINGAN TANAMAN ANGGREK (Dendrobium SP) PEBRA HERIANSYAH; TRINOP SAGIARTI; ROVER ROVER
Jurnal Agroteknologi Vol 5, No 1 (2014): Agustus 2014
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v5i1.1142

Abstract

The study was carried out at the Laboratory of Plant Biotechnology Faculty of Agriculture, University of islam Riau Pekanbaru. The timing of the study during the three months from October to December 2013. The design used in this study is the factorial experiment in completely randomized design (CRD), which consists of two factors and three replications. The first factor is the provision of Myoinositol: A0 (0 mg/l), A1 (25 m/l), A2 (50 mg/l), and A3 (75 mg/l).While the second factoris the provision of active charcoal: B0 (0 g/l), B1 (1 g/l), B2 (2 g/l), and B3 (3 g/l). From the results of this study concluded that the provision of various concentration of Myoinositol treatment singely provide significant effect on all parameters of the observations with the best treatment A2 (50 mg/l Myoinositol administration) that age emerged shoots (20,25days), sum up shoot (2,11 fruit) shoot height (2,32 cm), sum up roots (3,00 fruit) and weight wet roots (26,39 mg). Treatment provision of various consentrations of active charcoal.Singely provide significant effect on all parameters of the observations with the best treatment B2 (1 g/l active charcoal administration) that age emerged shoots (23,92 days), sum up shoot (2,06 fruit)shoot height (2,01 cm), sum up roots (2,67 fruit)and weight wet roots (25,59 mg). Interactions are granting various concentrations of Myoinositol and active charcoal provide significant effect of the parameters of the observation age emerged shoots with the best treatment in the combination treatment A2B0 (16,33 days), and weight wet roots with the best treatment A2B0 (47,47 mg) Myoinositol, Arang Aktif, Anggrek.
MULTIPLIKASI EMBRIO SOMATIS TANAMAN ANGGREK (Dendrobium sp) DENGAN PEMBERIAN KINETIN DAN SUKROSA SECARA IN-VITRO PEBRA HERIANSYAH
Jurnal Ilmiah Pertanian Vol. 15 No. 2 (2019): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v15i2.1974

Abstract

The objective of the study was to obtain the combination of sucrose and kinetin concentration reducted in the best effect on Dendrobium explant. This research was conducted in laboratory of tissues culture in Balai Benih Induk Dinas Pertanian and Peternakan Provinsi Riau Pekanbaru from April to June 2015. The experiment was designed using completely randomized design (CRD), factorial consisting of two factors with three replications, the first factor was sucrose with four level respectively 0, 25, 50,75 g L-1 medium. The second factor was kinetin with four level : 0, 0,1, 1,0, 10 mg L-1. The optimum interaction between effect of sucrose and kinetin for percentage of life explants, age bud formation, percentage of explant forming buds, the number of shoot, a percentage of explant producing root was 50 g L-1 sucrose and 1,0 mg L-1 kinetin. Application of sucrose significantly effect age of bud formation, percentage of explant forming buds, number of shoot, shoots high, percentage of explant producing root, number of root, root high, root and shoot ratio (T/R Ratio). Sucrose at 50 g L-1 medium being the best consentration and Kinetin 1,0 mg L-1 being the best consentration.
In-Vitro Rooting Induction On The Embryo Somatic Of Dendrobium Species From Riau Province Indonesia Pebra Heriansyah; Hasan Basri Jumin; Maizar Maizar
Paspalum: Jurnal Ilmiah Pertanian Vol 8, No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Winaya Mukti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35138/paspalum.v8i2.190

Abstract

Rooting induction is a very important stage in in-vitro for the anticipation of the extinction, In this paper, the rooting induction of embryo somatic dendrobium species from Riau Province Indonesia is studied using different sucrose concentrations (0 g L-1; 25 g L-1; 50 g L-1; 75 g L-1), and Kinetin (0 mg L-1; 0,1 mg L-1; 1,0 mg L-1; 10 mg L-1), in a modified MS medium containing half the regular concentration of macronutrients at pH 5.6, with 50 g L-1 sucrose and 1,0 mg L-1  kinetin was optimal for a percentage of explant producing root, a number of roots, root high, root and shoot ratio  (T/R Ratio).
RESPON PEMBERIAN CAIRAN INFUST RINGER LAKTAT TERHADAP PERTUMBUHAN JENIS AGLAONEMA SP YANG BERBEDA Gusti Marlina; Desta Andriani; Pebra Heriansyah; Seprido
Jurnal Agro Indragiri Vol. 7 No. 1 (2022): Jurnal Agro Indragiri
Publisher : Fakultas Pertanian, Program Studi Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32520/jai.v9i1.1853

Abstract

Penelitian ini memiliki tujuan jangka panjang yaitu mengatasi kelangkaan pupuk dengan memanfaatkan cairan infust yang belum termanfaatkan menjadi pupuk organik. Selain itu juga memenuhi kebutuhan masyarakat akan tanaman hias di propinsi Riau umumnya dan di Kabupaten Kuantan Singingi khususnya. Aglaonema merupan sala satu tanaman hias yang buming secara mendunia. Sedangkan tujuan khusus penelitian ini untuk mengetahui formulasi cairan infust yang tepat untuk pertumbuhan tanaman aglaonema yang berbeda. 2) untuk mengetahui seefisiensi cairan infust dalam meningkatkan pertumbuhan tanaman aglaonema. Penelitian ini menggunakan metode Rancangan Acak Kelompok (RAK) Faktorial. Faktor I adalah penggunaan aglaonema yang berbeda (AL) terdiri dari AL : Aglaonema Lipstik ALW : Aglaonema Green Whita AA : Aglaonema Adelia. Faktor II adalah pemberian cairan infust Ringer Laktata (RL) dengan perlakuan RL0 : tanpa pemberian ringer laktat, RL1 : pemberian ringer laktat 10 ml RL2 : pemberian ringer laktat 20 ml RL3 : pemberian ringer laktat 30 ml. Hasil penelitian ini diuji secara statistik dengan uji F, kemudian bila berbeda nyata dilanjutkan dengan uji BNJ (Beda Nyata Jujur) pada taraf 5%. Hasil penelitian dapat disimpulkan (1) Perlakuan terbaik secara tunggal penggunaan aglaonema yang berbeda adalah perlakuan Aglaonema Green White pada pengamatan muncul tunas 31,00 hari, tinggi tunas 11, 87 cm, jumlah daun 4,32 helai dan diameter batang 3, 69 cm (2) Perlakuan terbaik secara tunggal pemberian cairan infus Ringer Laktat (RL) adalah pada perlakuan RL0 (31,44 hari) untuk pengamatan muncul tunas, perlakuan RL1 (4, 30 helai) untuk pengamatan jumlah daun, dan perlakuan RL2 (3, 41 cm) untuk pengamatan diameter batang (3) Secara interaksi penggunaan aglaonema yang berbeda (AL) dan pemberian cairan infus ringer laktak (RL) perlakuan terbaik dapat dilihat pada perlakuan ALWRL0 34,00 hari pada pengamatan muncul tunas.
UJI TINGKAT KONTAMINASI EKSPLAN ANGGREK Bromheadia finlysoniana L.miq DALAM KULTUR IN-VITRO DENGAN PENAMBAHAN EKSTRAK TOMAT Pebra Heriansyah; Elfi Indrawanis
Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Vol 18 No 2 (2020): Jurnal Agroqua
Publisher : University of Prof. Dr. Hazairin, SH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32663/ja.v18i2.1502

Abstract

Bromheadia finlaysoniana L. Miq orchid, is a ground orchid whose population is threatened with extinction due to various human activities, so it needs to be reproduced using tissue culture. The purpose of this study was to determine the level of contamination in various types of explants and administration of tomato extract. The treatment used consisted of two factors, namely the combination of various types of explants and tomato extract, each of which consisted of several levels. The treatment of the first factor was the concentration of explants consisting of 4 types: seeds, leaves, stems and roots, each of which was combined with Tomato Extract at 4 levels: 0, 100 g / L, 200 g / L, and 300 g / L. Observation of the percentage of contamination showed that the best explants for tissue culture propagation of Bromheadia finlaysoniana L. Miq were seed and root explants with a contamination percentage of 36.81% and 37.50%. Observations for the percentage of fungal contamination parameters showed that single explants, with the best treatment were root explants with a contamination percentage of 17.36%. Meanwhile, the best treatment combination was found in root and seed explants without tomato extract, with a percentage of fungal contamination of 8.33%. Observations for the percentage of bacterial contamination, the best single explant treatment was stem explants, with a bacterial contamination percentage of 29.17%. Meanwhile, for the contamination treatment, the best treatment was found in seed and stem explants without giving tomato extract with a percentage of fungal contamination of 16.67%
Identifikasi Jamur Kontaminan pada Berbagai Eksplan Kultur Jaringan Anggrek Alam (Bromheadia finlaysoniana (Lind.) Miq Desta Andriani; Pebra Heriansyah
Agro Bali : Agricultural Journal Vol 4, No 2 (2021)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.811 KB) | DOI: 10.37637/ab.v4i2.723

Abstract

Perbanyakan tanaman anggrek secara generatif memiliki masalah karena biji anggrek tidak memiliki endosperm perlu diperbanyak menggunakan teknik kultur jaringan. Kontaminasi merupakan faktor pembatas dalam perbanyakan tanaman secara kultur jaringan. Penelitian ini bertujuan untuk mendeskripsikan jenis-jenis kontaminan yang terdapat pada kultur kalus Bromheadia finlaysoniana. Penelitian ini dilaksanakan dua tahap. Tahap pertama dilakukan kultur dari berbagai eksplan Bromheadia finlaysoniana menggunakan media Murashige dan Skoog dengan pengayaan hormon 6-BAP (Benzylaminopurine) 1 mg/l; NAA (Naphthalene acetic acid) 0,5 mg/l; 100 mg myo inositol; pyridoxine-HCl 0,5 mg/l; thiamine-HCl 0,1 mg/l; nicotinic-acid 0,5 mg/l; glysine 2 mg/l. Tahap kedua menghitung jumlah dan persentase jamur kontaminan serta melakukan pengamatan makroskopik dan mikroskopik. Karakter makroskopis jamur kontaminan yang diamati meliputi warna koloni, arah pertumbuhan koloni dan bentuk permukaan koloni hifa, karakter mikroskopik yang diamati meliputi bentuk hifa (bersekat/tidak bersekat) dan produksi spora. Penentuan jenis kontaminan dibandingkan dengan morfologi dari Pustaka. Hasil yang diperoleh adalah persentase kontaminasi tertinggi pada eksplan tangkai bunga yaitu 81 % dan jumlah kontaminan tertinggi terjadi pada eksplan daun sebanyak 28 koloni. Jamur kontaminan yang tumbuh didominasi jamur dengan warna putih dan abu-abu dengan bentuk permukaan kasar. dari karakter mikroskopik sebagian besar memiliki hifa bersekat/bersepta dan tidak memproduksi spora. Hasil pengamatan karakter makroskopik mikroskopik jamur kontaminan pada kultur jaringan beberapa eksplan anggrek ditemukan dari jenis Rhizoctonia sp dan Mucor sp.
IDENTIFIKASI ANGGREK ALAM PADA KAWASAN RAWAN GANGGUAN DI SUAKA MARGA SATWA BUKIT RIMBANG DAN BUKIT BALING RESORT KUANTAN SINGINGI Pebra Heriansyah; Seprido Seprido; Desta Andriani
Agro Bali : Agricultural Journal Vol 3, No 2 (2020)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.656 KB) | DOI: 10.37637/ab.v3i2.583

Abstract

Anggrek alam merupakan kekayaan yang dimiliki oleh suatu Kawasan, sehingga keberadaannya perlu diidentifikasi agar tidak terjadi kepunahan. Tujuan penelitian ini adalah untuk mengidentifikasi anggrek alam di Kawasan Rawan Gangguan di Suaka Marga Satwa Bukit Rimbang dan Bukit Baling Resort Kuantan Singingi. Metode pengumpulan data dalam penelitian ini menggunakan metode purposive sampling melalui tahapan identifikasi baik dengan cara pengambilan hasil foto atau gambar dan spesimen hasilnya akan diidentifikasi dengan bantuan buku identifikasi. Kesimpulan penelitian ini adalah ditemukan 12 jenis anggrek dan 10 genera di Kawasan Rawan Gangguan Suaka Marga Satwa Bukit Rimbang dan Bukit Baling, dari ketinggian 90 – 200 mdpl. Dari 12 jenis ini ada beberapa jenis yang terancam punah yaitu Grammatophyllum speciosum Blume hanya ditemukan di satu titik saja, Dendrobium aloifolium (Blume) Reichb.f, Robiquetia spathulata (Blume) J.J.Smith, Epigeneium sp, Cimbidium spp, ditemukan di 2 titik
PENGARUH BERBAGAI KOSENTRASI SUKROSA DAN NICOTINIC ACID TERHADAP PERTUMBUHAN SUBKULTUR TANAMAN ANGGREK Dendrobium sp Restu Saleh; Pebra Heriansyah; Tri Nopsagiarti
Jurnal Agro Indragiri Vol. 7 No. 2 (2022): Jurnal Agro Indragiri
Publisher : Fakultas Pertanian, Program Studi Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32520/jai.v7i2.1792

Abstract

Anggrek Dendribium sp merupakan tanaman hias yang sangat populer karena memiliki jenis yang beragam. Penelitian ini bertujuan untuk mengetahui Pengaruh Berbagai Konsentrasi Sukrosa dan Nicotinic Acid Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp.. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) Faktorial terdiri dari 2 taraf perlakuan (A= Sukrosa dan B= Nicotinic Acid) dengan 3 kali ulangan.Yaitu : A0 (Tanpa Sukrosa), A1 (Sukrosa 10 mg/l), A2 (Sukrosa 20 mg/l), A3 (Sukrosa 30 mg/l), dan B0 (Tanpa Nicotinic Acid), B1 (Nicotinic Acid 0,1 mg/l), B2 (Nicotinic Acid 0,5 mg/l), B3 (Nicotinic Acid 0,9 mg/l). Berdasarkan hasil penelitian pemberian berbagai konsentrasi Sukrosa secara tunggal berpengaruh terhadap parameter yang diamati, dimana perlakuan terbaik terdapat pada A2 dengan rata-rata jumlah akar 8,20 buah, jumlah daun 8,79 helai, panjang daun 1,84 cm, panjang akar 1,37 cm pada eksplan anggrek Dendrobium sp.. Untuk perlakuan berbagai konsentrasi Nicotinic Acid berpengaruh nyata terhadap jumlah akar, jumlah daun, panjang daun dan panjang akar eksplan anggrek Dendrobium sp, dan pertumbuhan eksplan anggrek Dendrobium sp yang terbaik terdapat pada perlakuan (B2) pemberian Nicotinic Acid sebanyak 0,5 mg/l kedalam media MS dengan rata-rata jumlah akar 7,92 buah, jumlah daun 8,79 helai, panjang daun 1,73 cm dan panjang akar 1,25 cm pada eksplan anggrek Dendrobium sp. Dari hasil penelitian diperoleh bahwa Interaksi berbagai konsentrasi Sukrosa dan Nicotinic Acid berpengaruh nyata terhadap parameter jumlah daun dengan perlakuan terbaik A2B2 Pemberian Sukrosa 20 mg/l dan Nicotinic Acid 0,5 mg/l MS) dengan rata-rata jumlah daun 8,79 helai pada eksplan anggrek Dendrobium sp.
Concentration Test for Ferrous Sulfate (FeSO4) and Thiamin in Murashige and Skoog Medium on The Orchid Sub-Culture Dendrobium SP By In-Vitro Nadia Ratna Sari; Elfi Indrawanis; Pebra Heriansyah
Jurnal Penelitian Pendidikan IPA Vol 9 No 3 (2023): March
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i3.3088

Abstract

The Dendrobium orchid is one type with special features and many variations. This study aims to determine the administration of various concentrations of ferrous sulfate (FeSO4) and Thiamin to Dendrobium sp orchid explants on Murashige And Skoog media. The design used in this study was a Factorial Completely Randomized Design (CRD) consisting of 2 treatment levels (F = FeSO4 and T = Thiamin) with three replications. Namely: F0 (Without FeSO4), F1 (FeSO4 26.8 mg/l ), F2 (FeSO4 27.8 mg/l), F3 (FeSO4 28.8 mg/l), and T0 (Without Thiamin), T1 (Thiamin 0.1 mg/l), T2 (Thiamin 0.2 mg/l) l), T3 (Thiamin 0.3 mg/l). Based on the study results, by giving various concentrations of ferrous sulfate (FeSO4) had a single effect on the shoots quantity parameters with the best treatment found in treatment with an average shoots quantity (.03 ea), F3 for shoot height parameters (1.37 cm), for the total leaves (6.14), for the roots quantity (5.17), and root length were found in treatment with an average of 1.29 cm on the Dendrobium sp. Orchid explants. For the treatment of various concentrations of Thiamin, the single effect on the number of shoots with the best treatment was found at an average (3.44 ea), for the shoot height parameter (0.98 cm), and for the leaves quantity (6.14 ea), for the root length parameter (1.28 cm). The interaction significantly affected the observed parameters, namely the number of shoots with treatment (giving FeSO4 28.8 mg/l and Thiamin 0.3 mg/l) with an average shoot quantity of 1.71 (ea).
Co-Authors A. HAITAMI Andriani, Desta Chairil Ezward, Chairil Deno Okalia Dewi Sukma Elfi Indrawanis Elfi Indrawanis Elfi Indrawanis, Elfi GUSTI MARLINA Gusti Marlina Hasan Basri Jumin Jeni Santika Maizar Maizar Nadia Ratna Sari Restu Saleh ROVER ROVER Sandra Arifin Aziz Seprido Seprido, Seprido Tri Nopsagiarti TRINOP SAGIARTI Wahyudi Wahyudi Waras Nurcholis