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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 342 Documents
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Screening of chitinase production medium by Fusarium solani isolated from endophytic karst using Two Level Factorial Design Musdalifah, Musdalifah; Rachmawaty, Rachmawaty; Alimuddin, Alimuddin; Pagarra, Halifah; Azis, Andi Asmawati; Masriany, Masriany
Biogenesis: Jurnal Ilmiah Biologi Vol 14 No 1 (2026)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v14i1.65554

Abstract

Chitinase is an important enzyme in various biotechnology applications, especially in biological control, so it requires increased induction efficiency. This study aims to determine the growth media factors that most influence the production of chitinase enzymes by the endophytic fungus Fusarium solani using the 2-Level Fractional method. Eight media variables are estimated to have an effect, namely the content of chitin colloid, ammonium sulfate, potassium sodium tartrate, KH2PO4, Na2HPO4, MgSO4·7H2O, CaCl2, and pH. Chitinase activity was evaluated using the DNS technique after four days of liquid fermentation and analyzed using the design express DX6 4.1 application. The results of the analysis of variance (ANOVA) showed that the second-order polynomial regression model was very significant (p <0.0001), with an R² value of 0.9515, which indicates that the model explains 95.15% of the data variation. Based on the screening results, it was found that ammonium sulfate, Na2HPO4, and MgSO4 7H2 were the most effective in increasing chitinase production. Optimization of media conditions resulted in an increase in chitinase activity of up to 18 U/mL. This research has the potential to be used as a basis for developing industrial-scale bioprocesses for the formulation of chitinase-based biopesticides and biopharmaceutical products.
PREVALENCE AND IDENTIFICATION OF BACTERIA CAUSING ASYMPTOMATIC BACTERIURIA IN PREGNANT WOMEN AT ANTANG COMMUNITY HEALTH CENTER, MAKASSAR CITY Hartati, Hartati; Misnarliah, Misnarliah
Biogenesis: Jurnal Ilmiah Biologi Vol 14 No 1 (2026)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v14i1.66301

Abstract

Asymptomatic bacteriuria in pregnancy carries significant risks to maternal and fetal health. Untreated cases can progress to symptomatic urinary tract infections, prematurity, low birth weight, and fetal death. Data on the prevalence and types of bacteria causing asymptomatic bacteriuria in pregnant women, especially in Makassar, are still very limited. This study aims to determine the prevalence of asymptomatic bacteriuria and the types of bacteria causing it in pregnant women at the Antang Community Health Center in Makassar. This study is a quantitative descriptive study, using microbiological examination methods through urine dipstick testing, urine culture, and identification. The subjects were 58 pregnant women without symptoms of urinary tract infections who underwent pregnancy checkups at the Antang Community Health Center in Makassar. The sample used was a mid-stream urine specimen. Based on the demographic characteristics of pregnant women in their first, second, and third trimesters at the Antang Health Center in Makassar City, it shows that the majority of respondents are young pregnant women in the final stages of pregnancy (third trimester). Based on the results of the dipstick examination, it shows that the majority of respondents do not have an increase in leukocytes, which indicates a low possibility of urinary tract infections among pregnant women in their first, second, and third trimesters at the Antang Health Center in Makassar City. The results of urine culture showed that 2 samples with positive dipstick results for leukocyte esterase and nitrite parameters had bacterial colonies of >100,000 CFU/mL. The prevalence of asymptomatic bacteriuria in pregnant women in their first, second, and third trimesters at the Antang Health Center in Makassar City in this study was 3.45%. The types of bacteria that cause it are Escherishia coli and Klebsiella pneumoniae.

Page 35 of 35 | Total Record : 342

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