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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 332 Documents
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Isolation and characterization of potential proteolytic and amylolytic bacteria from Bayanan hot spring as bioremediation agents Santosa, Slamet; Setiyaningrum, Erma Nur
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.41722

Abstract

Hot water temperature can be a place for the development of thermophilic bacteria. Thermophilic bacteria produce thermostable enzymes which are needed in various industrial fields such as agriculture, food, detergent, pharmacology, and bioremediation agents. This study aims to isolate and characterize potential proteolytic and amylolytic bacteria from the Bayanan hot spring. Samples were collected from two distinct locations within the geothermal environment: the hot spring source itself and the associated water storage pools. The collected samples underwent a dilution and isolation process to obtain pure bacterial cultures. Subsequently, these isolates were inoculated onto selective media, including skim milk agar (SMA) and nutrient agar (NA), to assess their proteolytic and amylolytic activities, respectively. The presence and extent of these enzymatic activities were determined by measuring the diameter of the clear zone surrounding each bacterial colony. The analysis revealed the presence of eight proteolytic bacterial isolates and twenty amylolytic isolates. Notably, a hydrolysis index threshold of ≥2.5 was implemented to identify isolates with high potential for proteolytic and amylolytic activity. Based on this criterion, two proteolytic bacterial isolates (codes B2-12-C3 and B1-12-B3) exhibited a proteolytic index exceeding 2.5. Furthermore, eight amylolytic isolates displayed an index above 2.5 (codes B1-10-A1, B1-10-A3, B2-8-A1, B2-8-A2, B2-10-A1, B2-10-A2, B2-12-C1, and B2-12-C2). These isolates, particularly those with high hydrolysis index values, has potential as bioremediation agents due to their demonstrated ability to efficiently hydrolyze proteins and starches.
The quality of liquid biostimulant formula of microbial-based during packaging and storage Radiastuti, Nani; Susilowati, Dwi Ningsih; Rahayu, Rizkiyah Putri; Kosasih, Jajang; Tentrem, Titi
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.42159

Abstract

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Diversity of tree vegetation in Mount Baung Natural Tourism Park utilization block, Pasuruan Regency Ilyasa, Muhammad Hasan; Suheriyanto, Dwi
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.42839

Abstract

The high diversity of tree species found in natural forests contributes to their ability to maintain ecological balance. Mount Baung Natural Tourism Park is the conservation area in Pasuruan Regency, East Java that functions to protect biodiversity. The research aims to identify tree species, analyze tree diversity, and determine tree dominance with the importance value index (IVI). The research was conducted from February to March 2023 in Mount Baung Natural Tourism Park utilization block (MBNTP UB), Pasuruan Regency, East Java, Indonesia. The observation location was determined to be 37 points and plots were made in the point with a size of 20 m × 20 m for the tree phase, 10 m × 10 m for pole phase, 5 m × 5 m for the sapling phase, and 2 m × 2 m for the seedling phase. Data collected from field observations were analyzed to determine the Shannon diversity index using PAST 4.01. Tree dominance was analyzed using IVI. The research showed that 14 species from 8 tree families are found in MBNTP UB. The Fabaceae family is found to be abundant compared to other families. The highest number of individuals found in the seedling phase is Cassia glauca. Swietenia macrophylla has the highest individual’s number in the sapling, pole, and tree phases. The highest tree diversity is found in the tree phase. Diversity at the tree phase is classified as moderate, while at the seedling, pole, and sapling phase the diversity is low.
The relationship between environmental factors and the diversity and abundance of flower-visitor insects on chili pepper in dry season Sururin, Fahma Wardah; Muzakhar, Kahar; Khafiyya, Nida An; Purwatiningsih, Purwatiningsih
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.42864

Abstract

Approximately 80% of flowering plants are highly dependent on insect pollination, including the chili pepper (Capsicum frutescens). Environmental conditions serve as parameters for insects in their activities. Therefore, this study was conducted to determine the diversity and abundance of flower-visiting insects in C. frutescens and their environmental factors. Field observations of flower-visiting insects were conducted during the dry season utilizing aerial capture methods. The data was analyzed using diversity, abundance, evenness, and dominance indices equations. Multiple linear regression analysis was also conducted to determine the abiotic and biotic factors (environmental factors) that influence the abundance of flower-visiting insects. The analysis results showed that a moderate trend in the diversity of insect and as many as 33% of the total recorded insect individuals belong to the Vespidae family. The Allorhynchium argentatum showed the highest species abundance (27% individuals). Based on the multiple regression analysis results, environmental factors had a non-significant native influence on the abundance of flower-visiting insects. Environmental conditions during the dry season have a weak influence on insect interactions with plants.
Quality Phytophthora de Bary and formerly named Pythium Pringsh. species isolated from woody ornamental hosts in Galician nurseries Northwest Spain Ares, Jose Luis Andrés
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.46769

Abstract

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Antioxidant potential of ethanol, ethyl acetate, n-hexane extracts from leaves, fruits, stems, and roots of red okra (Abelmoschus esculentus) Putri, Salsabilla Wiyana; Hapsari, Lukiteswari Dyah Tri; Junairiah; Wahyuningsih, Sri Puji Astuti
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.43666

Abstract

Abelmoschus esculentus L. Moench has been known for its abundant and affordable antioxidant potential. This plant has different antioxidant levels regarding the parts of the plant. Red okra is rich in flavonoids and polyphenols, including strong antioxidant as quercetin ad anthocyanin. This research is intended to determine the IC50 value and compare the total content of phenolic and flavonoid from ethanol, ethyl acetate, and n-hexane extract of red okra (A. esculentus) leaves, fruits, stems, and roots. Extraction process was done by gradual maceration method using ethanol, ethyl acetate, and n-hexane. IC50 value interprets the antioxidant activity obtained from DPPH test. The results showed a very strong antioxidant activity from red okra stem extract with IC50 of 25.22 ppm and the lowest from fruit n-hexane extract with 821.55 ppm. From TPC test, the highest content was obtained from stem ethanol extract with 156.01 mg GAE/g and the lowest from stem n-hexane extract with 25.99 mg GAE/. From TFC test, the highest content was obtained from fruits ethanol extract with 108.15 mg QE/g and the lowest from stem ethanol extract with 2.26 mg QE/g. In conclusion, this research showed that red okra (A. esculentus) contains a high level of phenol and flavonoid compound and the highest antioxidant content was shown in ethanol extract.
Shoots induction of axillary buds in blueberries (Vaccinium corymbosum L.) in vitro with the addition of BAP and IBA Restanto, Didik Pudji; Budha, Al Sura Tri; Prayoga, Mohammad Candra; Slameto, Slameto; Khozin, Mohammad Nur
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.46312

Abstract

Blueberry Vaccinium corymbosum L. is a fruit plant that has high economic value and many benefits. Blueberries in Indonesia are not yet in great demand so domestic needs are still met by imports. Conventional blueberry propagation is done using stem cuttings. The disadvantages of conventional propagation are that seedling growth is slow, requires a large source of planting material, and is ineffective. The aim of the research was to determine the effect of the combination of BAP and IBA hormones on the induction of axillary shoots in effective in vitro propagation of blueberries. The explants used were axillary buds of blueberry, sterilized using fungicide, bactericide, bayclin, sodium hypochlorite and alcohol. The research design used a completely randomized design (CRD) with a combination of BAP and IBA hormones. The BAP hormone consists of three concentrations, namely 0.5, 1.0, and 1.5 mg/L. The IBA hormone consists of three concentrations, namely 0, 0.1, and 0.2 mg/L. Based on research results, the combination treatment of BAP and IBA succeeded in inducing shoots, but was not able to induce roots. The BAP 0.5 mg/L treatment showed the best results with a shoot emergence rate of 4.33 weeks, shoot height of 1.68 cm, number of shoots 1.67, and number of leaves 12.67. The findings of this study highlight the potential of BAP and IBA hormone combinations in accelerating blueberry shoot induction, offering an alternative propagation method that could support domestic production and reduce dependence on imports.
Potensi Senyawa Bioaktif Andrographolide Pada Daun Sambiloto (Andrographis paniculata) Sebagai Kandidat Anti Photo-Aging Secara In Silico Zuhriyah, Nur; Fatkhurrohman, Fajar; Aida, Monika; Apriliani, Sindi; Setiyono, Eko
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.57447

Abstract

Skin aging caused by UV radiation has become a growing concern among the Indonesian population. The effects of UV-induced skin aging can be mitigated by antioxidant compounds. Although synthetic antioxidants are commonly used to combat aging, their long-term use is discouraged due to potential systemic side effects and carcinogenic risks. Therefore, natural antioxidant compounds are needed as a safer alternative. Andrographis paniculata, known as Sambiloto, contains a natural antioxidant compound called andrographolide. This study aims to evaluate the potential of andrographolide by determining its binding affinity toward target protein receptors involved in the photoaging mechanism triggered by increased levels of Reactive Oxygen Species (ROS). Through in silico analysis, the potential of andrographolide was assessed using reverse docking techniques with databases such as PubChem, PharmMapper, SwissTargetPrediction, SuperPred, and PASS Online. Subsequent molecular docking was performed using PyRx 0.8 and PyMol software. The in silico data revealed interactions between the ligand compound and its receptors, including binding activity and binding positions based on hydrophobic interactions and hydrogen bonds, which were further analyzed. The results showed that andrographolide binds effectively to the IL-1, NF-κB, and IL-6 receptors with relatively low binding affinities of -7.5 (IL-1), -6.6 (NF-κB), and -6.5 (IL-6). Low binding affinity indicates stronger and more stable interactions between the ligand and receptor. Based on the results, andrographolide from A. paniculata exhibits promising potential as a candidate for anti-photoaging agents that can serve as an alternative to synthetic antioxidants.
Protective effect of juwet fruit extract (Syzygium cumini L.) on duodenum histomorphometry and histopathology of male mice exposed to pyrantel pamoate Susilowati, Rina Priastini; Santoso, Adit Widodo; Sabini, Jasmine Harumi
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.56552

Abstract

Pyrantel pamoate, known as an anthelmintic under the brand name Combantrin®, is effectively used to kill worms in the intestines. However, long-term use of it has been associated with side effects and risk of drug resistance. Syzygium cumini L.is known for its antioxidant and anti-inflammatory which may support antiparasitic effects and tissue healing. This study aims to examine the effect of juwet (S. cumini L.) fruit water extract (JFWE) on the duodenum structure of mice suspected to be infected with worm, through histomorphometric analysis and histopathological examination. The pyrantel pamoate was only administered once after the acclimatization period, while the JFWE was administered for 3 weeks. A total of 30 male mice were divided into six groups: negative control (aquadest), positive control (pyrantel pamoate, Combantrin® 20 mg/mL), and JFWE at doses of 5 mg/mL, 10 mg/mL, 20 mg/mL, and 40 mg/mL, administered for 3 weeks. Histomorphometric and histopathological evaluations were conducted by microscopically observing the duodenum structures, including intestinal villi, and the thickness of the mucosa, submucosa, and muscularis layers. The results showed a significant difference for the decrease in intestinal villi height and thickness of the mucosa, submucosa, and muscularis layers in JWFE doses of 5 mg/mL and 10 mg/mL administration group, compared to the negative control group (aquadest), the treatment groups of pyrantel pamoate, and JFWE doses of 20 mg/mL and 40 mg/mL (p<0.05). The conclusion is that the administration of juwet fruit water extract doses of 20 mg/mL and 40 mg/mL can improve the histomorphometry structure and also the histopathology of the duodenum of male mice.
Biofertilizers improve growth rate, nitrate reductase activity, and productivity of shallot (Allium cepa L.) under drought stress Lena Mardiana; Siswanti, Dwi Umi
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.57317

Abstract

Shallot (Allium cepa L.) is one of Indonesia's horticultural crops and represents as superior commodity. However, shallots are highly susceptible to drought and require sufficient water for growth. Biofertilizers contain various microbes that provide nutrients and increase the resistance of shallots to drought stress. This study aimed to determine the effect of biofertilizers on the growth rate, nitrate reductase activity, and productivity of shallot plants under drought stress. The research was conducted using a Factorial Completely Randomized Design with two factors. The first factor was the doses of biofertilizer with 0, 10, 15, and 20 L ha-1. The second factor was the drought stress with treatments of 25, 50, 75, and 100% field capacity. Parameters measured included plant height rate, leaf number rate, number of tillers, tuber wet weight, tuber dry weight, and nitrate reductase activity. Data were analyzed by ANOVA using SPSS followed Duncan’s Multiple Range Test (DMRT) at 5% significance level. The results showed that biofertilizer application did no significantly different on the growth rate, but significantly affected the number of tillers, tuber weight, and nitrate reductase activity of shallots under drought stress. The optimum dose of 10 L ha-1 biofertilizer increased the number of tillers (9.33). The optimum dose of 20 L ha-1 biofertilizer increased the growth rate, tuber wet weight (4.46 g), tuber dry weight (0.63 g), and nitrate reductase activity (1.11 µmol NO2- g-1 leaf wet weight h-1 of incubation). It is concluded that biofertilizer application, particularly at 10–20 L ha-1, can improve shallot performance under drought stress and is recommended as a drought mitigation strategy in shallot cultivation.

Page 32 of 34 | Total Record : 332

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