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INDONESIA
Indonesian Journal of Agricultural Science
Published by Kementerian Pertanian
ISSN : 1411982X     EISSN : 23548509     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The journal publishes primary research articles from any source if they make a significant original contribution to the experimental or theoretical understanding of some aspect of agricultural science in Indonesia. The definition of agricultural science is kept as wide as possible to allow the broadest coverage in the journal. Accredited base on Director Decree of Indonesian Institute of Science No. 816/D/2009 with acreditation No. 172/AU1/P2MBI/08/2009. IJAS is newly accredited by the Indonesian Institute of Science in 2012 (No. 473/AU2/P2MI-LIPI/08/2012) for period of 2012-2015.
Arjuna Subject : -
Articles 352 Documents
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AN ALTERNATIVE METHOD OF REMOTE SENSING AND GIS FOR ASSESSING AGRICULTURAL DROUGHT IN UPPER BRANTAS WATERSHED, INDONESIA Rizatus Shofiyati; Kiyoshi Honda; Hidayat Pawitan
Indonesian Journal of Agricultural Science Vol 12, No 2 (2011): October 2011
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v12n2.2011.p70-83

Abstract

In Indonesia, drought disasters have been reoccurring more frequently in recent years. The 1997-1998 El Nino had caused the worst drought to Indonesia in the last 50 years and disrupted rice production. Remote sensing (RS) and geographic informa-tion system (GIS) provide good capability to achieve spatially distributed information over wide area coverage and multi-temporal data to give sufficient information to anticipate those situations. The study aimed to develop a method using GIS combined with satellite data for monitoring and assessing agri-cultural drought in Brantas Watershed, Indonesia. The drought factors were determined based on expert knowledge analysis. Risk assessment method was developed using weighting which is determined based on significant factors of drought, i.e. rainfall pattern, irrigation status, ground water capacity, soil drainage, and land cover. Satellite data were used to analyze the characteristics of temporal variations of normalized difference vegetation index (NDVI) against drought factors. Weighting scores were determined by analyzing NDVI character using changes in NDVI and normal line diagram of each factor. The accuracy of drought risk map was evaluated by comparing drought risk level and NDVI value. The results indicated that expert knowledge analysis of the drought factors showed significant influence on NDVI value. Drought risk and drought status showed a high positive correlation with R2 = 0.85 for NOAA AVHRR, meaning that there is a significant correlation between the two (r = 0.92). The results of this study can be used to determine spatially location of drought-prone areas based on bio-physical factor causes. Therefore, it can be make recom-mendation for prevention of agricultural drought in the future.
THE POTENTIAL USE OF SSR MARKERS TO SUPPORT THE MORPHOLOGICAL IDENTIFICATION OF INDONESIAN MUNGBEAN VARIETIES Reflinur Reflinur; Puji Lestari; Suk-Ha Lee
Indonesian Journal of Agricultural Science Vol 17, No 2 (2016): October 2016
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v17n2.2016.p65-74

Abstract

Mungbean varieties were mainly characterized based on morphological traits. Molecular genetic approach is expected to help the breeder in identification of mungbean varieties in more detail and to protect intellectual property right. This study aimed to identify Indonesian mungbean varieties based on DNA fingerprint profile using a marker set to support morphological characters. A total of 22 Indonesian mungbean accessions were characterized based on 21 morphological traits and 55 simple sequence repeats (SSRs) primers. Of the total 22 mungbean varieties used in the present study, 16 varieties were improved varieties and remaining six varieties were local varieties originated from Java, Nusa Tenggara and Sulawesi collected in GeneBank of ICABIOGRAD. The results showed that the 21 morphological characters were not sufficient to differentiate 22 mungbean varieties, while SSR analysis revealed that eight multi-alleles markers and high polymorphic information content (PIC) values have been successfully selected for varietal identification. The selected markers enabled to differentiate each mungbean variety according to their genetic marker with the lowest distance of 0.125, demonstrating the robustness of the selected marker set as a tool to identify a specific DNA fingerprint profile as a varietal identity (ID). The genetic identity of a variety was shown by digital barcoding which represented a series of alleles produced by corresponding markers. The DNA fingerprint profile of each variety would be beneficial as reference identities of a mungbean variety. 
STABILITY OF ANTHOCYANIN DURING PROCESSING, STORAGE AND SIMULATED DIGESTION OF PURPLE SWEET POTATO PASTA Ira Mulyawanti; Slamet Budijanto; Sedarnawati Yasni
Indonesian Journal of Agricultural Science Vol 19, No 1 (2018): June 2018
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v19n1.2018.p1-8

Abstract

Purple sweet potato is rich in anthocyanin giving a potential application in food product development.  However, anthocyanin is relatively unstable and easily degraded during processing and storage. Understanding the stability and bio-accessibility of anthocyanin during processing, storage and simulated digestion is very important. The study aimed to investigate changes in anthocyanin degradation during processing, storage and simulated digestion of purple sweet potato pasta. The pasta was prepared through several processing steps, i.e. steaming the tuber, steaming the dough formula, extrusion, drying and boiling. Anthocyanin was analyzed at every stages of processing and storage of the pasta. The durability of the pasta during storage was analysed using an accelerated shelf-life testing method at 30, 40 and 50ºC for 28 days. The study showed that anthocyanin content decreased during the whole stages of processing and storage, but slightly increased during steaming. The highest loss of the anthocyanin occurred in the boiling process. Based on resistance to stomach and intestinal conditions, the bio-accessibility of anthocyanin was better in the digestive system in the stomach than that in the intestines. The increased anthocyanin appeared again in the colon. This study provides useful information for designing an effective method to minimize an extensive loss of anthocyanin of purple sweet potato for food product development.
BIOMASS PRODUCTION AND FORMULATION OF Bacillus subtilis FOR BIOLOGICAL CONTROL Amran Muis
Indonesian Journal of Agricultural Science Vol 7, No 2 (2006): October 2006
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v7n2.2006.p51-56

Abstract

Bacillus subtilis is a widespread bacterium found in soil, water, and air. It controls the growth of certain harmful bacteria and fungi, presumably by competing for nutrients, growth sites on plants, and by directly colonizing and attaching to fungal pathogens. When applied to seeds, it colonizes the developing root system of the plants and continues to live on the root system and provides protection throughout the growing season. The study on biomass production and formulation of B. subtilis for biological control was conducted in the laboratory of Department of Plant Pathology, College of Agriculture, University of the Philippines Los Baños (UPLB-CA), College, Laguna from May to July 2005. The objective of the study was to determine the optimum pH and a good carbon source for biomass production of B. subtilis and to develop a seed treatment formulation of B. subtilis as biological control agent. Results showed that the optimum pH for growth of B. subtilis was pH 6 (1.85 x 109 cfu/ml). In laboratory tests for biomass production using cassava flour, corn flour, rice flour, and brown sugar as carbon sources, it grew best in brown sugar plus yeast extract medium (6.8 x 108 cfu ml-1 in sterile distilled water and 7.8 x 108 cfu ml-1 in coconut water). In test for bacterial biomass carriers, talc proved to be the best in terms of number of bacteria recovered from the seeds (3.98 x 105 cfu seed-1).
BROWN ROOT ROT DISEASE OF CASHEW IN WEST NUSA TENGGARA: DISTRIBUTION AND ITS CAUSAL ORGANISM Supriadi Supriadi; E.M. Adhia; D. Wahyuno; S. Rahayuningsih; N. Karyani; M. Dahsyat
Indonesian Journal of Agricultural Science Vol 5, No 1 (2004): April 2004
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v5n1.2004.p32-36

Abstract

Brown root rot disease is a major constraint on cashew plantation in Pekat District, West Nusa Tenggara. Its causal agent has not been characterized. This paper describes efforts to study the pathogen, distribution and loss. Field study was conducted in Pekat District in 2003. Laboratory  experiments to isolate and test the causal agent were conducted in the Indonesian Spices and Medicinal Crops Research Institute, Bogor.  Research results showed that the disease was found widespread in several villages in Pekat District, such as Pekat, Beringin Jaya, Sorinomo, and Nangamiro. Total number of died cashew trees was 1,075 equals to 5,106 kg kernel yield lost, worth Rp20.5 million. Infected trees showed leaf yellowing and defoliation leading to die. The lateral and taproots near collar were encrusted with gravel, earth, and brown mycelia sleeves. The fungus produced arthrospores and brown pigmentation on agar medium containing 0.05% gallic acid. An isolate of the fungus induced typical disease symptoms following inoculation on 5 month-old cashew seedlings. These results indicated that the causal agent of mass decline of cashew in Pekat District is Phellinus noxius. In field, the fungus also infects a barrier tree (Lannea coromandelica [Houtt.] Merr.) (Anacardiaceae), locally known as kedondong pagar or kayu bantenan.
POTENTIAL USE OF ENDOPHYTIC BACTERIA TO CONTROL Pratylenchus brachyurus ON PATCHOULI Rita Harni; Supramana Supramana; Supriadi Supriadi
Indonesian Journal of Agricultural Science Vol 13, No 2 (2012): October 2012
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v13n2.2012.p86-95

Abstract

Pratylenchus brachyurus is an important parasitic nematode which significantly decreases quality and quantity of patchouli oil. One potential measure for controlling the nematode is by using endophytic bacteria. These bacteria also induce plant growth. This study aimed to evaluate the potential of endo-phytic bacteria to control P. brachyurus. The experiments were carried out in the Bacteriological Laboratory of the Plant Protection Department, Bogor Agricultural University, and the Laboratory and Greenhouse of the Indonesian Spice and Medicinal Crops Research Institute from April to December 2007. Endophytic bacteria were isolated from the roots of patchouli plants sampled from various locations in West Java. Antagonistic activity of the isolates were selected against P. brachyurus and their abilities to induce plant growth of patch-ouli plants. Isolates having ability to control P. brachyurus and promote plant growth were identified by molecular techniques using 16S rRNA universal primers. The results showed that a total of 257 isolates of endophytic bacteria were obtained from patchouli roots and their population density varied from 2.3 x 102 to 6.0 x 105 cfu g-1 fresh root. As many as 60 isolates (23.34%) were antagonistic against P. brachyurus causing 70-100% mortality of the namatode, 72 isolates (28.01%) stimu-lated plant growth, 32 isolates (12.47%) inhibited plant growth, and 93 isolates (36.18%) were neutral. Based on their antago-nistic and plant growth enhancer characters, five isolates of the bacteria, namely Achromobacter xylosoxidans TT2, Alcaligenes faecalis NJ16, Pseudomonas putida EH11, Bacillus cereus MSK, and Bacillus subtilis NJ57 suppressed 74.0-81.6% nema-tode population and increased 46.97-86.79% plant growth. The study implies that the endophytic bacteria isolated from patchouly roots are good candidates for controlling P. brachyurus on patchouly plants. Bahasa IndonesiaPratylenchus brachyurus adalah nematoda parasit pada tana-man nilam yang dapat menurunkan hasil dan kualitas minyak nilam. Salah satu cara pengendalian yang potensial terhadap nematoda tersebut adalah menggunakan bakteri endofit. Selain dapat membunuh nematoda, bakteri endofit juga dapat meng-induksi pertumbuhan tanaman. Penelitian bertujuan untuk  mengevaluasi potensi bakteri endofit yang berasal dari tanaman nilam untuk mengendalikan namatoda parasit P. brachyurus. Penelitian dilakukan di Laboratorium Bakteriologi, Departemen Proteksi Tanaman, Institut Pertanian Bogor, serta di  laboratorium dan rumah kaca Balai Penelitian Tanaman Rempah dan Obat, pada bulan April sampai Desember 2007. Bakteri endofit diisolasi dari sampel akar tanaman nilam dari beberapa lokasi di Jawa Barat. Isolat-isolat bakteri endofit diseleksi kemampuannya untuk membunuh P. brachyurus dan menginduksi pertumbuhan tanam-an nilam. Isolat bakteri endofit yang potensial selanjutnya diidentifikasi secara molekuler menggunakan primer universal 16S rRNA. Penelitian memperoleh 257 isolat bakteri endofit dengan kerapatan populasi 2,3 x 102 sampai 6,0 x 105  cfu g-1 berat basah akar. Enam puluh isolat (23,34%) di antaranya bersifat antagonis terhadap P. brachyurus dengan mortalitas 70-100%, 72 isolat (28,01%) dapat memacu pertumbuhan tanaman nilam, 93 isolat (36,18%) bersifat netral, dan 32 isolat (12,47%) dapat menghambat pertumbuhan tanaman. Berdasarkan hasil peng-ujian antagonis dan pemacu pertumbuhan tanaman, lima isolat bakteri, yaitu Achromobacter xylosoxidans TT2, Alcaligenes faecalis NJ16,  Pseudomonas putida EH11, Bacillus cereus MSK, dan Bacillus subtilis NJ57 dapat menekan populasi nematoda 74,0-81,6% dan meningkatkan pertumbuhan nilam 46,97-86,79%. Penelitian mengindikasikan bahwa bakteri endofit dari tanaman nilam berpotensi mengendalikan P. brachyurus pada tanaman nilam.
EFFECT OF GAMMA IRRADIATION ON THE GROWTH AND DEVELOPMENT OF SAGO PALM (Metroxylon sagu Rottb.) CALLI Imron Riyadi; Sumaryono Sumaryono
Indonesian Journal of Agricultural Science Vol 17, No 1 (2016): April 2016
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v17n1.2016.p35-40

Abstract

The application of gamma irradiation on plant materials may increase the genetic variation of the offspring with useful traits. The experiment was conducted to determine the effect of irradiation dosage of gamma ray on growth and development of sago palm (Metroxylon sagu) calli. Friable calli of sago palm derived from suspension culture were used as a material source. The primary calli were initiated from apical meristematic tissues of sago palm suckers of Alitir variety from Merauke, Papua. The treatments used were dosage of gamma ray irradiation at 0, 5, 10, 15, 20 and 25 Gy. The treated calli were then subcultured on modified Murashige and Skoog (MMS) solid medium containing 3% sucrose and 0.1% activated charcoal and added with 1 mg l-1 2,4-D and 0.1 mg l-1 kinetin. The results showed that at all irradiation dosages, calli biomass increased significantly. The highest proliferation of calli biomass of 5.33 folds from the initial culture after 4 weeks was achieved at gamma irradiation of 25 Gy, whereas the lowest proliferation of calli biomass of 3.4 folds was achieved at control. The best development of embryogenic calli was obtained at 10 Gy that produced 100% somatic embryos, whereas the lowest somatic embryo formation at 0% was obtained at 0 and 25 Gy after one subculture. High response of somatic embryo induction to gamma irradiation at 10 Gy may increase production of somatic embryos. These results can be used in in vitro breeding of sago palm via mutagenesis to create new elite varieties.
THE GENOTYPES X ENVIRONMENT INTERACTION FOR STARCH YIELD IN NINE-MONTH OLD CASSAVA PROMISING CLONES Sholihin Sholihin
Indonesian Journal of Agricultural Science Vol 10, No 1 (2009): April 2009
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v10n1.2009.p12-18

Abstract

Cassava (Manihot esculenta) is planted in dry areas with different environmental conditions, therefore the yield is varied. The aim of the study was to analyze the genotype x environment interaction for starch yield in 9-month old cassava promising clones. The experiment was conducted on mineral soils in four different locations, i.e. Lumajang-East Java (Inceptisols), Kediri-East Java (Entisols), Pati-Central Java (Alfisols), and Tulangbawang-Lampung (Ultisols) during 2004- 2005. The experiment was arranged in split plot design with three replications. The main plots were cultivation techniques, i.e. simple technology and improved technology, whereas the subplots were 15 cassava promising clones. Starch yield of 9- month old cassava plants was analysed using the additive maineffects and multiplicative interaction (AMMI). The results showed that environmental factors determined the stability of starch yield were soil bulk density on subsoil, the number of rainy days at fifth month, minimum air temperature at fourth month, and minimum air humidity at seventh month. CMM97002-183, Adira 4, CMM97007-145, CMM97007-235, Malang 2, CMM97002-36, and CMM97006-44 were identified as the stable cassava clones for starch yield in 9-month old. Average starch yield of Adira 4 was the third after MLG 10311 and CMM 97006-52. The CMM97006-52 was adapted to the soils having high P2O5 content on topsoil, high minimum air temperature at 4 and 5 months after planting, high minimum relative humidity at 7 months after planting, low total rainfall at 5 months after planting, and low number of rainy days at 5 and 8 months after planting. MLG 10311 was adapted to low soil bulk density. The average starch yield of MLG 10311 was the highest at 9 months after planting. The study implies that advanced trials for CMM 977006-52 and MLG 10311 clones are needed, so the clones can be released as new varieties of cassava. In selection and evaluation, the bulk density on subsoil is needed to be attained specifically to increase the probability to obtain new variety of cassava.
CHARACTERIZATION AND QUALITY ASSESSMENT OF INDONESIAN COMMERCIAL BIOFERTILIZERS Edi Husen Husen; R.D.M. Simanungkalit; Rasti Saraswati; Irawan Irawan
Indonesian Journal of Agricultural Science Vol 8, No 1 (2007): April 2007
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v8n1.2007.p31-38

Abstract

Biofertilizers currently sold in the market are not labeled with a distinct quality standard. As such, farmers may buy low quality biofertilizers, which can reduce their profit and trust on the benefit of biofertilizers. This paper presents the characteristics of various products of commercial biofertilizers as well as farmers’ knowledge and experience on the products. The study was carried out in 2004-2006 by collecting and analyzing data on registered commercial biofertilizers, checking their availability at the market (39 agricultural shops), and interviewing farmers on their knowledge and experience on the use of biofertilizers on various farming systems in Bandung District, West Java (86 respondents) and Semarang District, Central Java (77 respondents). The quality of biofertilizers was tested in the laboratory based on microbial density and its functional (phenotypic) traits. The study showed that amongst various brands of biofertilizers commercialized, 41 brands of them have been officially registered as commercial products. Two brands of other biofertilizers found in agricultural shops were registered as organic or inorganic fertilizers. In general, each biofertilizer contained two or more microbial strains and was claimed to have multiple functions. However, most of them (>90%) were not labeled with expiry date information. Macronutrient contents (NPK) of some microbial carriers were almost equal to those of organic fertilizers. Around 38% of respondents in Bandung knew biofertilizers and less than 10% have ever used them. In Semarang, however, familiarity and personal experience of the respondents were much lower, i.e. 10% and 3%, respectively. About 67% and 50% of agricultural shops in Bandung and Semarang sold biofertilizers, respectively. Laboratory analyses showed that microbial density of five biofertilizers tested was lower than that of product specification, although most of them were positive for N-fixing and P-solubilizing traits. Some microbial strains contained less than 103 cfu based on the dilution level testing. These figures imply the urgent need to improve the existing quality standard system of biofertilizers including its control mechanisms.
PRODUCTION OF POLYCLONAL ANTIBODY TO THE COAT PROTEIN OF CITRUS TRISTEZA VIRUS IN CHICKEN EGGS Nurhadi Nurhadi; Kamaruzaman Sijam; Inon Sulaiman
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v4n1.2003.p18-26

Abstract

Citrus tristeza virus (CTV) is one of the most destructive diseases in many citrus growing areas of Indonesia. Effective strategies for controlling CTV depend on diagnostic procedure namely enzyme-linked immunosorbent assay (ELISA). Study aimed to purify the CTV antigen and produced its polyclonal antibody. Virion of the severe CTV isolate designated UPM/ T-002 was concentrated by polyethylene glycol (PEG) precipitation combined with low speed centrifugation. Semipurified antigen was further purified by sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE). The specific coat protein (CP) band of CTV with molecular weight of 25 kD was excised and eluted using elution buffer containing 0.25 M Tris-HCl pH 6.8 + 0.1% SDS, then used as antigen for injection into 6-month-old female of White Leghorn chicken. Results, showed than the specific polyclonal antibody raised against the 25-kDa CP had a titer of approximately 104, gave low background reaction with healthy plant sap and reacted specifically with CTV isolates. The reaction was equally strong for a severe, a moderate, a mild, and a symptomless isolate, suggesting a broad reaction range of this antibody toward different CTV isolates. Optimal virus titer can be obtained since virus loss during purification could be minimized and the highly purified antigen as an immunogen could be obtained by cutting out the CP band from SDS-PAGE gels. Large amount of highly titer of CTV antibody can be produced in chicken egg. The simplicity of the procedure makes it economically acceptable and technically adoptable because the antibody can be produced in basic laboratory.

Page 29 of 36 | Total Record : 352

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