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INDONESIA
Indonesian Journal of Agricultural Science
Published by Kementerian Pertanian
ISSN : 1411982X     EISSN : 23548509     DOI : -
Core Subject : Agriculture,
The journal publishes primary research articles from any source if they make a significant original contribution to the experimental or theoretical understanding of some aspect of agricultural science in Indonesia. The definition of agricultural science is kept as wide as possible to allow the broadest coverage in the journal. Accredited base on Director Decree of Indonesian Institute of Science No. 816/D/2009 with acreditation No. 172/AU1/P2MBI/08/2009. IJAS is newly accredited by the Indonesian Institute of Science in 2012 (No. 473/AU2/P2MI-LIPI/08/2012) for period of 2012-2015.
Arjuna Subject : -
Articles 352 Documents
INHIBITION OF THE GROWTH OF TOLERANT YEAST Saccharomyces cerevisiae STRAIN I136 BY A MIXTURE OF SYNTHETIC INHIBITORS Eny Ida Riyanti; Edy Listanto
Indonesian Journal of Agricultural Science Vol 18, No 1 (2017): June 2017
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v18n1.2017.p17-24

Abstract

Biomass from lignocellulosic wastes is a potential source for biobased products.  However, one of the constraints in utilization of biomass hydrolysate is the presence of inhibitors. Therefore, the use of inhibitor-tolerant microorganisms in the fermentation is required. The study aimed to investigate the effect of a mixture of inhibitors on the growth of Saccharomyces cerevisiae strain I136 grown in medium containing synthetic inhibitors (acetic acid, formic acid, furfural, 5-hydroxymethyl furfural/5-HMF, and levulinic acid) in four different concentrations with a mixture of carbon sources, glucose  (50 g.l-1) and xylose (50 g.l-1) at 30oC. The parameters related to growth and fermentation products were observed. Results showed that the strain was able to grow in media containing natural inhibitors (BSL medium) with µmax of 0.020/h. Higher level of synthetic inhibitors prolonged the lag phase, decreased the cell biomass and ethanol production, and specific growth rate. The strain could detoxify furfural and 5-HMF and produced the highest ethanol (Y(p/s) of 0.32 g.g-1) when grown in BSL. Glucose was utilized as its level decreased in a result of increase in cell biomass, in contrast to xylose which was not consumed. The highest cell biomass was produced in YNB with Y (x/s) value of 0.25 g.g-1. The strain produced acetic acid as a dominant side product and could convert furfural into a less toxic compound, hydroxyl furfural. This robust tolerant strain provides basic information on resistance mechanism and would be useful for bio-based cell factory using lignocellulosic materials. 
PROPERTIES AND MANAGEMENT IMPLICATIONS OF SOILS FORMED FROM VOLCANIC MATERIALS IN LEMBANG AREA, WEST JAVA Edi Yatno; Siti Zauyah
Indonesian Journal of Agricultural Science Vol 9, No 2 (2008): October 2008
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v9n2.2008.p44-54

Abstract

Soils formed from volcanic materials have a high potential for agricultural development, especially for horticultural crops, tea, and pine trees. Data on the characteristics of these soils are important for the management planning. Six representative soil profiles developed on andesitic volcanic ash and tuff in Lembang area, West Java were studied to determine the soil physical, chemical, and mineralogical properties, to study the relationship between the soil properties, and to classify the soils according to the Soil Taxonomy. The results indicated that all the soils had very deep (>150 cm) solum. In general, the volcanic ash soils were darker colored, more granular, more friable, less sticky and less plastic than the volcanic tuff soils. Physically, the ash soils had lower bulk density (0.44-0.73 mg m-3) and higher available water content (13-33%) than the tuff soils. Bulk density decreased with increasing allophane. Chemically, the ash soils had higher pHNaF (mostly > 10), higher organic carbon (4.3-6.8% in upper horizons), higher CEC (20- 44 cmolc kg-1), and higher P retention (> 85%) than the tuff soils. P retention logarithmically increased with increasing oxalate extractable Al and allophane. The sand fractions of the ash soils were dominated by hornblende, while the tuff soils were predominantly composed of opaque minerals. In the clay fractions, the ash soils were dominated by allophane, whereas the tuff soils showed high contents of gibbsite and metahalloysite. Soils developed on volcanic ash were classified as Thaptic Hapludands and Typic Melanudands, while soils formed from volcanic tuff were classified as Andic Dystrudepts. The low bulk density and friable consistency of the soils contributed to favorable soil tilth. However, high P retention and Al saturation in most soils are limiting factors for plant growth. Application of P fertilizers and liming coupled with efficient placement can be recommended to enhance P availability and reduce Al toxicity. Organic matter can be used to reduce Al toxicity. Soil conservation needs to be considered, especially in the steep slope areas.
EFFECT OF ALUMINUM ON PLANT GROWTH, PHOSPORUS AND CALCIUM UPTAKE OF TROPICAL RICE (Oryza sativa), MAIZE (Zea mays), AND SOYBEAN (Glycine max) D. Nursyamsi; M. Osaki; T. Tadano
Indonesian Journal of Agricultural Science Vol 1, No 2 (2000): October 2000
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v1n2.2000.p51-62

Abstract

Aluminum toxicity is the most limiting factor to plant growth on acid soils. Structural and functional damages in the root system by Al decrease nutrient uptake and lead to reduce plant growth and mineral deficiency in shoot. Greenhouse experiment was conducted to study the effect of Al on plant growth, and P and Ca uptake of rice, maize, and soybean. The plants were grown in hydroponic solution added with 0, 5, 10, and 30 ppm Al, at pH 4.0. The results showed that relative growth of shoots and roots of upland rice, lowland rice, maize, and soybean decreased with an increase of Al level. However, sometimes the low Al level (5 ppm) stimulated shoot and root growth of some varieties in these species. According to total AlRG30 values, which is Al concentration in solution when relative growth decreased to 50%, Al tolerance of species was in order of barley < maize < soybean < lowland rice < upland rice. For maize, Al tolerance was in the order of Arjuna < Kalingga < P 3540 < SA 5 < SA 4 < PM 95 A < SA 3 < Antasena; for soybean was Wilis < INPS < Galunggung < Kerinci < Kitamusume; for lowland rice was RD 23 < Kapuas < Cisadane < KDML 105 < IR 66 < RD 13, and for upland rice was Dodokan < JAC165 < Cirata < Orizyca sabana 6 < Danau Tempe < Laut Tawar. Based on the rank of Al tolerance, rice was the useful crop to be planted in acid soils. Antasena (maize), Kitamusume ( soybean ), RD 13 (lowland rice), and Laut Tawar (upland rice) were also recommended for acid soils. P and Ca concentration in shoots and roots commonly decreased with an increase of Al level. However, the low Al level stimulated absorption of P and Ca concentrations in shoots and roots.
EFFECT OF BIO FERTILIZER ON MATURE OIL PALM IN NORTH SUMATRA AND RIAU Y Taryo Adiwiganda; Balaman Tarigan; Berani Purba
Indonesian Journal of Agricultural Science Vol 7, No 1 (2006): April 2006
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v7n1.2006.p20-26

Abstract

Chemical fertilizers have routinely been applied at oil palm plantations to maintain soil fertility. Due to the increase of chemical fertilizer prices, some efforts have been done at such as to reduce cost and increase efficiency in chemical fertilizer application at oil palm plantations include the use of bio fertilizer (BF). The objective of experiment was to evaluate the effect of reducing oil palm standard dosage of chemical fertilizers combined with BF on mature oil palm yield. The experiment was carried out in 2002-2003 covering an area of 4367 ha at PTPN IV in North Sumatra and of 515.58 ha at PTPN V in Riau. The planting material was Dura x Pasifera variety and planted in 1980-1995. A randomized block design was used having three fertilizer treatments: (A) 100% chemical fertilizer (normal dosage), (B) 75% chemical fertilizer + BF, and (C) 50% chemical fertilizer + BF. The replications at PTPN IV and V were 11 and 7, respectively. The BF was applied firstly in May 2002. Dosage of BF was 250 g tree-1 yr-1. The BF was given in pockets with four pockets per palm tree. The parameters observed were fresh fruit bunch (FFB) weight, average of bunch weight, and average of bunch number. Results of the experiments showed that palm yield treated with 75% chemical fertilizer + BF and 50% chemical fertilizer + BF were both not significantly different with palm yield treated with 100% chemical fertilizer. The application of 75% chemical fertilizer + BF and 50% chemical fertilizer + BF gave benefit of Rp509,093 and Rp1,231,535 ha-1 yr-1, respectively. Technically, nutrients given as chemical fertilizers using combination of 75% chemical fertilizer + BF are still higher than the nutrients transported into 26 tons palm FFB ha-1 yr-1. Fertilizer treatment combination of 50% chemical fertilizer + BF will technically be safe only for palm yield of <16 tons palm FFB ha-1 yr-1, since nutrients given as fertilizer are still higher than those transported into the palm yield.
IMPROVEMENT OF SEEDLING ESTABLISHMENT OF WET SEEDED RICE USING GA3 AND IBA AS SEED TREATMENT Sri Wahyuni; Uma R. Sinniah; Mohd. Khanif Yusop; Rajan Amarthalingam
Indonesian Journal of Agricultural Science Vol 4, No 2 (2003): October 2003
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v4n2.2003.p56-62

Abstract

Direct seeding has some advantages compared to transplanting system in rice, however there are some constraints need to be addressed. One  constraint generally faced in the wet seeded rice is poor stand  establishment. The experiment was conducted to improve seedling  establishment by using selected growth regulators. Seeds of four rice  cultivars, i.e. Memberamo, Widas, MR 84, and MR 219 were treated with  GA3 (gibberellic acid-3) of 25, 50, and 100 mg 1-1, and IBA (indole-3  butyric acid) of 10, 20, and 40 mg l-1. Results showed that cultivar  response to GA3 was similar in all parameters observed, however the response of cultivars to IBA was different at the initial stage. In Indonesian cultivars (Memberamo and Widas), shoot length of IBA treated seedlings was comparable to the control, but the IBA treated seedlings of  Malaysian cultivars (MR 84 and MR 219) had slightly shorter shoots   compared to the control. GA3 as seed treatment induced favorable early emergence and shoot growth in the initial stage, but the shoots were  etiolated and resulted in susceptible to lodging even in the vegetative  stage. In comparison, exogenous application of IBA resulted in high number of adventitious roots contributing towards better establishment of seedling with broader and greener leaves. The growth regulator was lost its  effect by 2 or 3 weeks after sowing. All IBA concentrations also did not  show any significant differences on growth parameters or have any detrimental effect on grain yield in all cultivar tested. IBA 10 mg l-1 was  sufficient in providing the required improvement in the rice seedlings.
THE EFFECT OF PICLORAM AND LIGHT ON SOMATIC EMBRYOGENESIS REGENERATION OF PINEAPPLE Ika Roostika; Nurul Khumaida; Ika Mariska; Gustaaf Adolf Wattimena
Indonesian Journal of Agricultural Science Vol 13, No 2 (2012): October 2012
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v13n2.2012.p43-53

Abstract

Smooth Cayenne is the largest pineapple type cultivated in Indonesia, but its vegetative planting materials for mass propagation are limited. Somatic embryogenesis is a potential method to be applied. The aim of this study was to investigate the somatic embryogenesis regeneration under the effect of picloram and light. Callus formation was induced by picloram (21, 41 and 62 μM) added with 9 μM thidiazuron. The calli were transferred onto MS or Bac medium  enriched with N-organic compounds with or without addition of 21 μM picloram under dark or light condition. The compact calli were subcultured onto MS medium supplemented with 4.65 μM kinetin, while the friable calli were  transferred onto BIG medium (modified MS + 1.1 μM benzyl adenine + 0.9 μM indole butyric acid + 0.09 μM giberelic acid) or B medium (MS + 0.018 mM benzyl adenine). The results showed that the events of somatic embryogenesis were started from cell polarization, asymmetrical division, proembryo formation as  embryogenic tissues and friable embryogenic tissues, and embryo development. The best treatment for callus induction was 21 μM picloram. The addition of 21 μM picloram on N-organic enriched medium and the use of light condition  proliferated embryogenic calli. The N-organic enriched Bac medium and light condition yielded the highest number of mature somatic embryos (17 embryos perexplant in 2 months). The B medium was better than BIG medium to develop  somatic embryos from friable embryogenic tissues. The somatic embryogenesis method presented is potential for pineapple mass propagation and artificial seedproduction.Abstrak Bahasa IndonesiaSmooth Cayenne merupakan kultivar nenas yang banyak dibudidayakan di  Indonesia, namun ketersediaan benih untuk perbanyakan massal masih terbatas. Embriogenesis somatikadalah metode yang potensial untuk produksi bibit secara massal. Tujuan penelitian adalah untuk mempelajari pengaruh pikloram dan pencahayaan terhadap regenerasi embriogenesis somatik nenas. Kalus diinduksi menggunakan pikloram (21, 41, dan 62 μM) dan penambahan thidiazuron 9 μM. Selanjutnya, kalus dipindahkan ke media MS atau Bac yang diperkaya dengansenyawa N-organik dengan atau tanpa penambahan pikloram 21 μM dalam kondisi gelap atau dengan pencahayaan. Kalus kompak disubkultur pada media MS yang mengandung kinetin 4,65 μM, sedangkan kalus remah dipindahkan ke media BIG (MS modifikasi + bensil adenin 1.1 μM + indole butyric acid 0,9 μM + giberelic acid 0,09 μM) atau media B (MS + bensil adenin 0,018 μM). Hasil penelitian  menunjukkan bahwa tahapan embriogenesis somatik diawali dengan polarisasi sel, pembelahan asimetris, pembentukan proembrio sebagai jaringan embriogenik danjaringan embriogenik remah, serta perkembangan embrio. Perlakuan terbaik untuk induksi kalus adalah pikloram 21 μM. Penambahan pikloram 21 μM pada media yang diperkaya dengan senyawa N-organik mampu meningkatkan jumlah kalusembriogenik. Media Bac yang diperkaya senyawa N-organik dan kondisi pencahayaan menghasilkan jumlah embrio somatik dewasa terbanyak (17 embrio per eksplan dalam 2 bulan). Media B lebih baik daripada media BIG untuk regenerasi embrio somatik dari jaringan embriogenik remah. Metode embriogenesis somatik yang dihasilkan dari penelitian ini berpotensi diterapkan untukperbanyakan massal dan produksi benih nenas.
IRON DYNAMICS AND ITS RELATION TO SOIL REDOX POTENTIAL AND PLANT GROWTH IN ACID SULPHATE SOIL OF SOUTH KALIMANTAN, INDONESIA Wahida Annisa; Dedy Nursyamsi
Indonesian Journal of Agricultural Science Vol 17, No 1 (2016): April 2016
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v17n1.2016.p1-8

Abstract

Organic matter has a function to maintain reductive conditions and to chelate toxic elements in acid sulphate soils. The study aimed to assess the dynamics of ferrous iron (Fe2+) in acid sulphate soil and its correlation with soil redox potential (Eh) and plant growth. The experiment was arranged in two factorial randomized block design with three replications. The first factor was two types of organic matter: (1) control (without organic matter), (2) rice straw and (3) rush weed (Eleocharis dulcis). The second factor was time of decomposition of organic matter: I1 = 2 weeks, I2 = 4 weeks, I3 = 8 weeks, and I4 = 12 weeks (farmer practice). The results showed that concentration of ferrous iron in the soil ranged from 782 to 1308 mg kg-1 during the rice growing season. The highest constant rate of iron reduction (k F2+) was observed on application of rice straw and rush weed with decomposition time of 8 weeks with the k Fe2+ value of 0.016 and 0.011 per day, respectively, while the ferrous iron formation without organic matter had the k Fe2+ value of 0.077 per day. The ferric iron (Fe3+) reduction served as a function of soil Eh as indicated by the negative correlation of ferrous iron and Eh (r = -0.856*). Organic matter decreased exchangeable iron due to chelating reaction. Iron concentration in roots was negatively correlated with soil soluble iron (r = -0.62*). Application of rice straw decomposed for 8 weeks increased the height of rice plant up to 105.67 cm. The score of Fe2+ toxicity at 8 weeks after planting ranged from 2 to 3, so rice crop did not show iron toxicity symptoms. 
RESPONSE OF POTATO TO POTASSIUM FERTILIZER SOURCES AND APPLICATION METHODS IN ANDISOLS OF WEST JAVA Nikardi Gunadi
Indonesian Journal of Agricultural Science Vol 10, No 2 (2009): October 2009
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v10n2.2009.p65-72

Abstract

An experiment to determine the response of potato to potassium (K) fertilizer sources and application methods in Andisols of West Java was conducted at a farmer’s field in the highland area of Pangalengan (1400 m asl.), West Java from August to November 2004. The treatments consisted of two K fertilizer sources (potassium chloride-KCl and potassium sulphate-K2SO4), two K rates (150 and 250 kg K2O ha-1), and three application methods (single, split, and split combined with foliar application), were arranged in a randomized complete block design with four replications. In the single application treatment, K was applied at planting, while in the split application treatment the K was applied half rate at planting and the rest at 6 weeks after planting (WAP). In the split combined with foliar application treatment, the K fertilizer was applied half rate at planting, a quarter rate at 6 WAP and another quarter rate by foliar spraying at 7, 8 and 9 WAP. The results showed that plant height was not significantly affected by the treatment (P >0.05). However, the sources and application methods of K fertilizer affected canopy cover, crop cover weeks (CCW), tuber dry weight (DW), and total plant DW at 10 WAP. Potatoes supplied with K2SO4 either in split or split combined with foliar application had significantly higher percent canopy cover, CCW, tuber DW, and total plant DW than those supplied with K fertilizer in single application. Potatoes supplied with K2SO4 had a higher tuber yield compared to those fertilized with KCl, especially under split or split combined with foliar application. To attain the same level of tuber yield as in the split combined with foliar application method, the rate of K2SO4 should be increased from 150 to 250 kg K2O ha-1 when using single application. It is therefore suggested that K2SO4 for potatoes should be used in split application combined with foliar application.
CHANGES OF SUCROSE CONTENT AND INVERTASE ACTIVITY DURING SUGARCANE STEM STORAGE Tri Agus Siswoyo; Ika Oktavianawati; Djenal Djenal; Bambang Sugiharto; Untung Murdiyanto
Indonesian Journal of Agricultural Science Vol 8, No 2 (2007): October 2007
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v8n2.2007.p75-81

Abstract

Invertases (beta-D-fructofuranosidase, E.C. 3.2.1.26) are the key enzymes involved in sucrose metabolism in sugarcane plants. They are highly correlated with sucrose and reducing sugar contents during plant growth. The sugarcane plants have two kinds of invertases, namely neutral invertase (NI) and acid invertase (AI). They have different function in sucrose accumulation. The research aimed to study the role of AI and NI in accumulation of reducing sugar during storage of sugarcane stems. Plant materials of 18-month-old field grown sugarcane of the commercial variety R-579 (wet-land) and M 442-51 (dry-land) were used. Three internodes were sampled to represent immature (internode 1-8/F1), maturing (internode 9-16/F2), and mature (internode 17-24/F3) stem tissues. All tissues were stored for 0-9 days at room temperature (28-32oC) and each day, the samplewas extracted to determine invertase activity, total soluble protein, and sugar contents. This observation was valid for invertase activity expressed on a protein basis. At the initiateharvested (0-3 days), NI had a higher specific activity than AI in the sucrose-accumulating region of the sugarcane stems. Negative significant correlation was found between NI specific activity and sucrose accumulation (r2 = 0.41, P < 0.05). AI showed a higher specific activity after 4 days harvested and had negative correlation with sucrose accumulation (r2 = 0.40, P < 0.05). These results showed that NI could be more  responsible in sucrose hydrolisis than AI at early storage of sugarcane stems.
COLORIMETRIC MEASUREMENTS OF COCOA BEANS (Theobroma cacao) Charles C.E. Meulemans; Untung Surapati T.; Ardin Tjatjo
Indonesian Journal of Agricultural Science Vol 3, No 2 (2002): October 2002
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v3n2.2002.p52-57

Abstract

A series of studies have been carried out to develop a simple, easy to use, and affordable device to measure the colour of cocoa beans. The present paper reports the development of the device based on colorimetric principles. An electronic device which enables cocoa smallholders to give an objective “onefigure” indication of the quality of their cocoa beans was described. A sensor was constructed which contained a red light emitting diode (LED) illuminating the target and a silicon photodiode detecting the reflected light. Because of the considerable difference in colour between dried unfermented and fermented cocoa beans, the differences in diffuse reflection of red light (660 nm) could be about 20 and very easy to measure. The method was checked with a spectrophotometer in the fixed mode at 660 nm, which revealed a high correlation coefficient. The sensor was combined with a microprocessor controlled electronic device for fast measurement of cocoa beans without any calculations which automatically counts the number of measured beans and gives the resulting average reflection value.

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