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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 6 Documents
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Search results for , issue "Vol 4, No 1 (2008): April" : 6 Documents clear
Identitas dan Keragaman Genetik Begomovirus yang Berasosiasi dengan Penyakit Keriting pada Tomat Berdasarkan Teknik Polymerase Chain Reaction (PCR)- Restriction Fragment Length Polymorphism (RFLP) Tri Joko Santoso; Sri H. Hidayat; M. Herman; H. Aswidinnoor; Sudarsono Sudarsono
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n1.2008.p9-17

Abstract

Begomoviruses, members of the Geminivirus,are considered as emerging plant viruses. This was due tothe increasing incidences and severities of the diseases in anumber of economically important crops, including tomato.Genetic diversities of the Begomovirus isolates infectingtomato (Lycopersicon esculentum) of several areas in Indonesiawere analyzed by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP)technique. A 1500 base pairs of PCR fragments amplified byusing degenerate primers for Begomovirus was digestedusing four restriction enzymes, i.e., DraI, EcoRI, RsaI, andPstI. The pattern of RE digested fragments of 8 Begomovirusisolates and the predicted RFLP fragments of the Begomovirusisolates in the GeneBank database were used to determinethe genetic identities and diversities among the isolates.Positive results of the PCR amplifications proved thatdiseased tomato plant samples collected from 8 locations inJava and Sumatra were infected with at least one Begomovirusisolate. The PCR amplification products, which weredigested using the four restriction enzymes indicated thepresence of polimorfisms among the DNA fragments of theBegomovirus isolates. Identifications of the Begomovirusindicated that the Brastagi, Bogor, Sragen, Ketep, and Boyolaliisolates were Tomato Leaf Curl Virus (ToLCV); theisolates from Malang and Blitar isolates were AgeratumYellow Vein Virus (AYVV), while one isolate from Kaliurangwas Tomato Yellow Leaf Curl Virus (TYLCV). Results of thephylogenetic analysis of the 8 Begomovirus isolates basedon Begomoviruses from the DNA database indicated thatthey belonged to three different groups.
KOMUNIKASI PENDEK Perbanyakan Tanaman Artemisia annua secara In Vitro Rossa Yunita; Endang G. Lestari
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n1.2008.p41-44

Abstract

Artemisinin, an anti-malarial medicineisolated from the annual wormwood Artemisia annua,has a marked activity against chloroquine-resistant andchloroquine-sensitive strains of Plasmodium falciparum.This compound is useful for treatment of cerebral malaria.An in vitro propagation system for A. annua has been developed.Shoots were induced by culturing seeds of A.annua on a MS medium containing BAP (0, 0.1, 0.3, 0.5mg/l). Shoots were also formed on each seedling culturedon the same medium. Root formations were obtained fromshoots that were subcultured on a MS medium containingIBA (0, 1.0, 1.5, 2 mg/l). The results showed that MS mediumsupplemented with BAP 0.3 mg/l was the best medium forinduction and multiplication of the shoots, while the MSmedium supplemented with IBA (1 mg/l) was good for rootformations.
Development and Characterization of F2 Population for Molecular Mapping of Aluminum-Toxicity Tolerant QTL in Soybean I Made Tasma; Ahmad Warsun; Asadi Asadi
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n1.2008.p1-8

Abstract

Keracunan aluminium merupakan salah satukendala utama dalam budidaya kedelai pada lahan masam.Pembentukan populasi F2 merupakan langkah awal yangmenentukan keberhasilan program pemuliaan tanaman. Tujuanpenelitian ini untuk membentuk dan mengkarakterisasipopulasi F2 hasil persilangan tetua toleran dan tetua pekakeracunan Al. Pembentukan populasi dilakukan menggunakanbantuan marka SSR. Dengan marka SSR populasi dapatdibentuk dengan cepat, akurat, dan efisien. Skrining genotipakedelai pada tanah masam kahat hara menghasilkan duagenotipa toleran dan dua peka. Empat persilangan tunggaldibuat untuk mendapatkan benih F1. Tanaman F1 dan F2 diidentifikasimenggunakam marka SSR Satt_070. Dua populasi(B3462 X B3293 dan B3462 X B3442) dipilih berdasarkansuperiotas fenotipa pada lahan masam dan karakteristik molekulerpasangan tetua. Karakterisasi kedua populasi di lapangmenunjukkan transgresiveness luas untuk karakter reproduksiseperti jumlah polong dan berat 100 biji. Ini mengindikasikanbahwa karakter penting lain selain karakter ketahananterhadap keracunan Al potensial untuk dipetakandari populasi ini. Metoda pembentukan populasi ini akan sangatbermanfaat bagi pemulia tanaman khususnya pemuliakedelai untuk meningkatkan efisiensi program pemuliaanketahanan terhadap keracunan Al.
Isolasi Identifikasi Bakteri Penghasil Xilanase serta Karakterisasi Enzimnya Nur Richana; Tun T. Irawadi; Anwar Nur; Khaswar Syamsu
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n1.2008.p24-34

Abstract

Xylanase is an extracellular enzyme produced bymicroorganisms. This enzyme is able to hydrolise xylane(hemicellulose) to produce xylooligosaccharide and xylose.Thermoalkaliphilic xylanase is an agent that can be used asa substitute in the pulp whitening process instead of chlorine.A study was done to isolate, identificate of bacteria andcharacterize xylanase. The isolation of xylanase producingbacteria has been done from soil and waste of starch industry.Colonies which produced clearing zone were presumedas xylanolytic bacteria and chosen for further screening.Identification of potential isolate in xylanase production wasdone using 16S ribosomal RNA sequencing. Isolate Bacilluspumilus RXA-III5 originated from lime or alkaline soil wasmore potential isolate in xylanase production than other 24isolates. Precipitation of xylanase, that was done usingammonium sulphate followed by dialyzes produced xylanaseof a higher specific activity (267.1 U.mg-1) than that usingacetone (131.1 U.mg-1) and ethanol (186.65 U.mg-1). Xylanasewas done at purification produced three fractions of xylanase.Xylanase characteristics consist of pH and temperature(9 and 50oC), Km and Vmaks value 6 mg.ml-1 and 0.2mol.minute-1, respectively. The Fe2+ was the strongest activetorand Mg2+ was the strongest inhibitor activity. This enzymewas detected as a cellulose-free xylanase. Xylanase is aprospective agent for bio-bleaching of paper.
Pengujian Nomor-nomor Harapan Padi Tahan Al dan pH Rendah Hasil Seleksi In Vitro dengan Kultur Hara Ragapadmi Purnamaningsih; Ika Mariska
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n1.2008.p18-23

Abstract

Rice productivity in acid soil is very low because of low pH,low availability of N, P, K, Ca, Mg, Mo, toxicity of Al and Mn.Development of Al tolerant varieties could increase riceproductivity in acid soil. Somaclonal variation and in vitroselection method can be used to develop new Al tolerancevarieties. A rapid screening method is needed to select alarge number of new genotypes or new inbred lines in plantbreeding, such as solution culture methods to evalu-ate Altolerantrice. This methods was used to know the responseto Al in the seedling stage, root development, and pHchanging. In this experiment solution culture method wasused to evaluate the new genotypes derived from somaclonalvariation and in vitro selection methods. These newgenotypes have been tested the tolerance characteristic byusing AlCl36H2O at 6 concentrations (0, 100, 200, 300, 400,and 500 ppm). Yoshida solution with two Al concentrationwere used to tested these genotypes. Measurement of Altolerance was based on root development by using RelativeRoot Length (RRL), the relativity of root length at 45 ppm and0 ppm. Almost all of the genotypes have RRLs higher than0.7, which means that there was a positive correlationbetween the in vitro method and solution culture method. Inthis experiment pH changes were not applicable to measurethe tolerance of the rice genotypes to Al and low pH.
ULASAN Kajian Filogenetika Molekuler dan Peranannya dalam Menyediakan Informasi Dasar untuk Meningkatkan Kualitas Sumber Genetik Anggrek Topik Hidayat; Adi Pancoro
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n1.2008.p35-40

Abstract

Early informationresulted from molecular phylogenetic studies of many importantornamental crops is often less attention to manygrowers and farmers. Phylogenetics is one of the most preferablemethod in systematics to reconstruct evolutionaryrelationships of groups of biological organisms in order tounderstand their biodiversities. This has been revolutionizedby DNA sequences data. In this method, a group of organismsthat shares many identical characteristics are consideredto be closely related; deriving from a commonancestor and is assumed to have similar genetic patternsand biochemical properties. By these basic principles,molecular phylogenetics plays important roles in revealing abasic knowledge on pattern of relationships to whichgenetic resources can be improved. Over the past decade,botanists have done several thousand phylogenetic analysesbased on molecular data of economically and horticulturallyimportant crops. Orchids are the best example for this.There is no doubt that most orchid plants had played roles inhorticulture and hybridization. At present, many infragenericand intergeneric hybrids are available commercially. Successfulhybridization can be achieved if two or more individualplants understudy are closely related in respect to theirgenetics and evolution.

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