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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 252 Documents
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Molecular Analyses and Resistance Evaluation to Bacterial Leaf Blight on Swampy Rice Genotypes Ahmad Dadang; Siti Yuriah; Nonon Saribanon; Joko Prasetiyono; Dwinita Wikan Utami
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p95-104

Abstract

Swampland is a prospective marginal land to be developed for rice cultivation. Rice plants that are adaptive to swampland usually have good tolerance to Fe. Previously, we obtained several rice lines tolerant to Fe toxicity in the field. This study aimed to analyze the swampy rice genotypes using STS molecular markers linked to bacterial leaf blight (BLB)-resistant genes and evaluate the resistance level of the genotypes against three BLB races under the greenhouse condition. This research was conducted in the Molecular Biology Laboratory and Greenhouse of the Indonesian Center for Agricultural Biotechnology andGenetic Resources Research and Development from January to May 2021. Eighteen swampy rice varieties/lines and two check varieties for Fe toxicity and BLB, respectively, were used in this research. The eight STS molecular markers linked to BLB resistance genes were used for genotyping analysis. The results showed that all rice plants tested contained xa5 allele gene, 19 genotypes had Xa4 allele gene, 17 genotypes had Xa7 allele gene, 9 genotypes had xa13 allele gene, 5 genotypes had xa13 allele gene, and 6 genotypes had Xa4 and Xa21 allele genes. Line B146E-4 was estimated to have 7 resistance alleles genes, meanwhile lines B14301E-KA-17-a, B14315E-KA-1, and B13-1-a have 6 resistance allele genes. The resistance evaluation of the genotypes against the three BLB races (races III, IV, and VIII) under the greenhouse condition showed that line B14315E-KA-1 was confirmed to be highly resistant to BLB disease. This line can be a promising candidate for swampy rice that is resistant toBLB disease.
Rapid Multiplication of Sugarcane Seedlings of PSJT 941 Variety from Tissue Culture Using Ethephon and Paclobutrazol Arinta Rury Puspitasari; Aprilia Mutia Dewi; Sri Winarsih
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p105-112

Abstract

Indonesia's sugar self-sufficiency was targeted since 2014 but the target has not been reached yet. One of the major constraints is the limited healthy seedlings available for new sugarcane area expansion. The mass production of healthy sugarcane seedlings may be achieved by rapid multiplication of tissue culture-derived seedlings using plant growth regulators (PGR) applied in the nursery house. The aim of this study was to determine the effect of ethephon and pacloburazol (PBZ) on the growth and seedling multiplication of sugarcane PSJT 941 variety. The research was carried out in the nursery house at Pasuruan Experimental Station, Indonesian Sugarcane Research Center. This study was arranged using a randomized block design with three replications. The treatments included (a) 3.5 ml/l ethephon, (b) 7.0 ml/l ethephon, (c) 1.5 ml/l PBZ, (d) 3.0 ml/l PBZ, and (e) control (without application of neither ethephon nor PBZ). The results showed that the highest number of shoots per clump was obtained from 3.0 ml/l PBZ treatment (8.7 shoots/clump) after 3 months of application. In the contrary, PBZ reduced the plant height compared with control and ethephon. Ethephon and PBZ treatments improved seed multiplication 8–52 times, much higher than the control that produced seed multiplication value six times. Further study is necessary to obtain optimal PBZ concentration for better stem height by decreasing the PBZ inhibition effect on stem growth.

Page 26 of 26 | Total Record : 252

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