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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 252 Documents
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Back Matter JA Vol 15 No 1 AgroBiogen, Jurnal
Jurnal AgroBiogen Vol 15, No 1 (2019): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n1.2019.p%p

Abstract

Rice Anther Culture to Develop Double Haploid Population and Blast Resistant Lines Ambarwati, Dinar; Soemantri, Ida H.; Utami, Dwinita W.; Apriana, Aniversari; Moeljopawiro, Sugiono
Jurnal AgroBiogen Vol 5, No 2 (2009): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n2.2009.p71-77

Abstract

Penyakit blas pada padi yang disebabkanoleh cendawan Pyricularia grisea, merupakan salah satukendala dalam produksi beras. Sumber gen ketahanan terhadappenyakit blas dijumpai pada spesies padi liar Oryzarufipogon. Populasi silang ganda (BC2F3) turunan IR64 danO. rufipogon mempunyai QTL untuk sifat ketahanan terhadappenyakit blas. Untuk mempercepat perolehan tanamanhomosigot dari populasi tersebut, dilakukan kultur anterpada dua media induksi kalus: I1 (N6 + NAA 2 mg/l + kinetin0,5 mg/l + sukrosa 60 g/l + putresin 0,16 g/l) dan I2 (N6 +2,4-D 2 mg/l + sukrosa 50 g/l) dan dua media regenerasi: R1(MS + NAA 0,5 mg/l + kinetin 2 mg/l + sukrosa 40 g/l +putresin 0,16 g/l) dan R2 (MS + NAA 1 mg/l + kinetin 2 mg/l+ sukrosa 30 g/l). Kultur anter dilakukan pada sembilan genotipe,di mana tiga genotipe (149-16, 343, 337-13) memberikanrespon terbaik dalam produksi planlet hijau setelahdikulturkan pada media regenerasi R1. Dari 208 planlet hasilregenerasi diperoleh 42 planlet haploid ganda dari genotipe149-16, 11 planlet haploid ganda dari genotipe 343, dan 44planlet haploid ganda dari genotipe 337-13. Skrining ketahananblas di rumah kaca pada populasi haploid gandamenghasilkan 46 tanaman tahan terhadap ras 001, 33 tanamantahan terhadap ras 033, dan 79 tanaman tahan terhadapras 173. Sebanyak 28 tanaman bersifat tahan, baik terhadapras 001, 033, maupun 173 seperti halnya O. rufipogon.Galur-galur homosigot ini akan diuji di lapang untuk ketahanannyaterhadap penyakit blas dan karakter agronominya.
Molecular Analysis and Phenotypic Performances of BC3F2 Upland Rice Lines Containing Alt and Pup1 Loci Mizan, Muhammad Rauful; Wirnas, Desta; Tasliah, nFN; Hidayatun, Nurul; Prasetiyono, Joko
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p83-92

Abstract

The challenges in upland rice cultivation are phosphorous (P) deficiency and aluminum (Al) toxicity, each controlled by Pup1 and Alt loci, respectively. Pyramiding the two genes into Indonesian rice varieties were previously done through Marker-Assisted Backcrossing method to obtain BC3F2 populations. The aims of this study were to analyze the BC3F2 upland rice lines containing the Alt and Pup1 loci molecularly (foreground and background analyses) and to test their phenotypic performances. Genetic materials tested included Dupa variety (donor of Alt) and three improved Indonesian genotypes (Dodokan-Pup1, Situ Bagendit-Pup1, and Batur-Pup1) as recurrent parents, Kasalath (donor of Pup1), and 300 BC3F2 lines from Dodokan-Pup1+Alt, Situ Bagendit-Pup1+Alt, and Batur-Pup1+Alt, respectively. The rice genotypes were selected individually using modified Yoshida nutrient solution, followed by foreground and background analyses. 150 out of 300 seedlings were selected and maintained until harvest in the greenhouse. Foreground analysis using markers (RM1361, RM12031, and Kas46-2) and tiller number performances resulted in 18 plants from BC3F2 Dodokan-Pup1+Alt, 30 plants from BC3F2 Situ Bagendit-Pup1+Alt, and 25 plants from BC3F2 Batur-Pup1+Alt still carrying Alt and Pup1 loci. Background analysis using molecular markers showed that the best individual lines of BC3F2 were number 56 for BC3F2 Dodokan-Pup1+Alt, number 32 or 70 for BC3F2 Situ Bagendit-Pup1+Alt, and number 20 for BC3F2 Batur-Pup1+Alt. The selected lines having both both Alt and Pup1 loci in homozygote condition with highest number of tiller per plant which are useful genetic materials for developing upland rice variety tolerance to low P and Al toxicity.
Fron Matter JA Vol 15 No 2 AgroBiogen, Jurnal
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p%p

Abstract

Genetic Diversity of Butterfly Pea (Clitoria ternatea) from Indonesia Based on Flower and Yield Component Traits in Two Land Conditions Trixie A. Ulimaz; Debby Ustari; Virda Aziza; Tarkus Suganda; Vergel Concibido; Jutti Levita; Agung Karuniawan
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p1-6

Abstract

Genetic diversity among the butterfly pea genotypes is important information to support breeding program of this underutilized crop. The important characters to be targeted in the breeding program of this crop included yield and yield components of flowers that are strongly affected by the environment and have not been previously reported. This study aimed to determine the genetic diversity of butterfly pea (Clitoria ternatea L.) from Indonesia tested in two land conditions, namely dryland and former paddy fields, based on flower character and yield component traits. The results showed that butterfly pea accessions were divided into two main clusters with dissimilarity coefficient of 0.01–3.99 indicating wide genetic diversity across  accessions. The Mantel correlation showed that the genetic distance among accessions studied were not significantly correlated (r = 0.044, P = 0.8709). Based on principal component analysis (PCA), the eigenvalue ranged from 1.69 to 3.34 with a cumulative contribution of 72.64%. The traits that influenced genetic diversity in this study were flower length, weight of one fresh flower, total weight of pods, and weight of 100 seeds. The results of this study should be useful to support future butterfly pea breeding program.
Construction of Binary Vector and Transformation of Synthetic LcCsp Gene into Nipponbare Rice Genome by Agrobacterium tumefaciens Transformation Method Dina Sri Yulita; Aqwin Polosoro; Atmitri Sisharmini; Aniversari Apriana; Febi Nurilmala; Kurniawan Rudi Trijatmiko
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p25-34

Abstract

Cold shock protein (Csp) is an essential bacterial protein for increasing abiotic stress tolerance, especially cold stress. Several studies discovered that overexpression of the gene successfully improves the tolerances of several types of plant not only under cold stress, but also other abiotic stresses, e.g. hot and drought conditions. The objectives of this study were to construct a binary vector containing the LcCsp gene modified from Lactobacillus casei and transform the gene into Nipponbare rice genome. The native LcCsp gene sequence, however, has low GC content (46.7%) while rice as transformation target plant has 52% GC content. The native LcCsp gene sequence, therefore, was optimized to the level close to 52.7% similar to GC content of the rice genome. This LcCsp gene was synthesized by using DNA printing technology (gBlocks® Gene Fragments Entry, IDT). The synthetic LcCsp gene was successfully inserted into the pCAMBIA1300-int binary vector driven by Ubiquitin1 promoter and NOS terminator. The T-DNA cassette was successfully transformed into Nipponbare rice genome by Agrobacterium tumefaciens strain LBA4404 using immature embryo transformation protocol. A total of 51 T0 Nipponbare lines survived from hygromycin selections and 21 lines were successfully acclimatized. Molecular analysis of the candidate lines showed that all Nipponbare transgenic putative lines contain the LcCsp gene demonstrating high transformation efficiency of 11.8%. The rice lines resulted from this study should be further analyzed and might be useful for developing rice transgenic lines tolerance to heat, drought, or saline stress condition.
Back Matter JA Vol 15 No 2 Jurnal AgroBiogen
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p%p

Abstract

Genome-Edited Plants and the Challenges of Regulating Their Biosafety in Indonesia nFN Bahagiawati; Dani Satyawan; Tri J. Santoso
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p93-106

Abstract

Genome editing is a precise breeding technique to improve plant properties by editing specific genes that regulate desired trait. Genome editing techniques can be designed so that the resulting plant does not contain foreign genes and the resulting changes in DNA sequences cannot be distinguished from products obtained by conventional gene mutations which have been considered as safe and therefore unregulated. Thus, genome editing products in some countries are also not specifically regulated as GM products even though their assembly process uses recombinant DNA and genetic transformation. Brazil, likeIndonesia ratified the Cartagena Protocol, but it issued a special  regulation that provides dispensation for several types of genome editing products and exempts them from regulations that apply to transgenic plants. The steps taken by other countries in regulating genome editing products can be taken into consideration in drafting regulations in Indonesia, in order to create a conducive environment that supports the use of this potential technology while at the same time provides assurance regarding its safety to human health and the environment. The purpose of this review was to provide information onthe development of genome editing technologies in plant breeding, analyze its risks compared to that of conventional breeding, and compare its biosafety regulation in various countries to provide some considerations for drafting regulations on the risk assessment of genome editing products in Indonesia, as a ratifying country of the Cartagena Protocol.
Validating Plant Genes Involved in Pepper Yellow Leaf Curl Indonesia Virus Infection Using VIGS in Model Plant Nicotiana benthamiana Kusumawaty Kusumanegara; Masanori Kaido; Kazuyuki Mise
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p7-16

Abstract

Pepper yellow leaf curl disease caused by Pepper yellow leaf curl Indonesia virus (PepYLCIV) has become a challenge to chili pepper cultivation. Development of resistant variety by utilizing recessive resistance gene is expected to control the disease in the field. This study aimed to validate three plant genes, namely deltaCOP, hsc70, and BAM1, in PepYLCIV infection by applying Virus-induced Gene Silencing (VIGS) in a model plant, wild type Nicotiana benthamiana. PepYLCIV and construct of Tobacco rattle virus (TRV) which induced silencing of each gene were co-inoculated into N. benthamiana plants through agroinfiltration. Gene expression and the relative amount of viral DNA were determined by quantitative reverse transcription PCR (qRT-PCR) and quantitative PCR (qPCR), respectively, at 15 days post inoculation. The results showed a decreased level of deltaCOP, hsc70, and BAM1 expressions to 66.4%, 53.0%, and 47.0%, respectively, compared to that in the control (100%). Silencing of the three genes decreased the accumulation of PepYLCIV to 0.1%, 18.4%, and 63.0%, respectively, compared to that in the control. deltaCOP and hsc70 genes were indicated to be involved in the viral infection and could be good candidate genes for obtaining chili pepper varieties resistant to PepYLCIV. This result affirmed that the reverse genetics technique could be an alternative approach for identifying plant genes involved in viral infection, including PepYLCIV. The use of an infectious clone in this study allows the virus inoculations could be carried out without rearing and maintaining its natural vector, hence reduces the risk of virus transmission to healthy plants.
Confirmation of Alleles Inheritance in F1 Progenies Derived from a Cross of Calcutta-4 (Musa acuminata ssp. burmannicoides) and Musa acuminata ssp. microcarpa Based on SSR Markers Dea Rosalia; Puji Lestari; Andy Soegianto; Darmawan Saptadi; Agus Sutanto; Kristianto Nugroho; Rerenstradika T. Terryana; I Made Tasma; Ika Roostika
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p17-24

Abstract

Banana breeding to produce improved varieties with disease resistance haracters and other desired traits could sustain its yield. Alleles harbored by parents could be passed on to the offsprings through hybridization, but need to be confirmed using molecular markers. This study aimed to confirm allele inheritance in F1 progenies derived from a cross of Calcutta-4 (Musa acuminata ssp.  burmannicoides) and M. acuminata ssp. microcarpa based on SSR markers. Eleven pairs of SSR primers were used to amplify DNA of 44 progenies using the PCR technique. The results showed that six SSR markers (MaSSR 1.1, MaSSR5.1, MaSSR 6.1, MaSSR 7.1, MaSSR 8.1, and MaSSR 11.1) were polymorphic in both parents. Four markers (MaSSR 1.1, MaSSR 5.1, MaSSR 6.1, and MaSSR 8.1) had PIC >0.7, indicating their informativeness to distinguish these progenies and other genetic studies of banana germplasms. A total of 44 F1 individuals were confirmed to harbor alleles inherited from their parents,suggesting as true progenies from the cross of Calcutta-4 and M. acuminata ssp. microcarpa. This population demonstrated 100% success of hybridization performed. Chi-Square analysis revealed that segregation of all markers did not match to Mendelian ratio 1:2:1, except for MaSSR 1.1 (x2 = 5,62) and MaSSR 6.1 (x2 = 3,77) markers. The genetic traceability of banana F1 progenies demonstrating the usefulness and feasibility of SSR markers in this study provided information on selection of true progenies which may be valuable for breeders to assist selection process in future banana breeding program in Indonesia.

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