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Biotropika
Published by Universitas Brawijaya
ISSN : 23027282     EISSN : 25498703     DOI : 10.21776/ub.biotropika.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biotropika: Journal of Tropical Biology invites research articles, short communication, and reviews describing new findings/phenomena of biological sciences in tropical regions, specifically in the following subjects, but not limited to biotechnology, biodiversity, microbiology, botany, zoology, biosystematics, ecology, and environmental sciences.
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Articles 22 Documents
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Search results for , issue "Vol 2, No 3 (2014)" : 22 Documents clear
Single Cell Protein Production using Yeast Isolates from Soy Factory Waste by Co-culture Method Corin, Chintya; Ardyati, Tri
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

Nutrient content in soy waste able to allow growth of yeasts. Yeast isolates have potency to be used in the production of single cell protein. The research was carried out to study isolates for production of single cell protein using co-culture method and to determine the effect of co-culture method in protein content obtained. The isolates used were I2YP5K1, I2YP5K2, KYP6K1, KYP3K2, AYP6K1, AYP6K2, and AYP5K4. Steps used in this research were grown the yeast isolates in Yeast Malt Broth, assay of antagonist among yeast isolates, single cell protein production, and identification using API 20C AUX. Parameters measured were biomass, number of cells, and protein content. Design of research using two-way ANOVA with three replications. Isolates able to be used for co-culture method in the production of single cell protein were KYP3K2 (Saccharomyces cereviseae), AYP6K1, AYP6K2, and AYP5K4. Co-culture methods does not increase biomass and number of cells, however able to increase the protein content. Protein production by co-culture isolates KYP3K2 and AYP6K2 is highest than other co-culture isolates with protein content 1.31 mg / g.   Key words : Co-culture, single cell protein, soy waste, yeast.
Karakteristik Vegetasi Riparian dan Interaksinya dengan Kualitas Air Mata Air Sumber Awan Serta Salurannya di Kecamatan Singosari Malang Ekki Totilisa Rachmawati; Catur Retnaningdyah
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : University of Brawijaya

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Abstract

The aims of this research are to know the characteristics of riparian vegetation based on QBR (Qualitat del Bosc de Ribera) analysis and profile of water quality (pH, DO, temperature, conductivity, turbidity) as well as to know the interaction between biotic index of the riparian vegetation and water quality in Sumber Awan Spring and its channel. Observations of riparian vegetation and water quality were conducted at seven stations. The correlation between quality of riparian vegetation and water quality was determined by Pearson correlation analysis using SPSS v.16. The results of this study indicate that the quality of riparian vegetation in the spring (station one) was the best from all downstream stations. This is indicated by the highest value of QBR (60), species richness (27 species) and species diversity index (>3). Downstream from the spring, especially five and six stations (residential area) showed lowest riparian quality, indicated by decreasing of diversity and QBR index value.The pH and DO value had fulfilled the standard for class I and class II respectivelybased on Indonesia Governmental Regulation No. 82/2001. When shrubs and tree species are high, the low value of turbidity was occurs in the water. Therefore, when the QBR score was high, the conductivity value will low in the water. Then, when pH was low, the richness of shrubs, tree species, and QBR score will high. According to QBR and water quality, Sumber Awan ecosystem was degraded. Keywords : riparian vegetation, Sumber Awan spring water, water quality
Skrining Bakteri Asam Laktat asal Susu Kambing Peranakan Etawa sebagai Penghasil Bakteriosin Indah Nur Fitria; Tri Ardyati
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : University of Brawijaya

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Abstract

Bakteri asam laktat (BAL) merupakan mikroorganisme yang tergolong GRAS (Generally Recognized as Safe). Sebagian besar  BAL memiliki potensi sebagai penghasil bakteriosin. Bakteriosin merupakan senyawa protein yang disintesis oleh ribosom dan mampu menghambat pertumbuhan bakteri lainnya. Penelitian ini bertujuan untuk memperoleh BAL penghasil bakteriosin dan mempelajari daya hambat bakteriosin terhadap Staphylococcus aureus dan Salmonella thypimurium. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan tiga kali ulangan. Tahapan dilakukan dengan isolasi BAL asal susu kambing Peranakan Etawa (PE) pada media MRS ditambah dengan CaCO3 1%, karakterisasi isolat BAL, deteksi penghambatan  kultur BAL terhadap bakteri uji, uji aktivitas penghambatan bakteriosin (CFS) terhadap bakteri uji, uji sensitivitas bakteriosin terhadap enzim proteolitik, serta identifikasi BAL dengan menggunakan API 50 CHL test kit. Dua belas isolat diperoleh dari isolasi, namun hanya 6 isolat (SKE5, SKE7, SKE8, SKE9, SKE10, dan SKE11) diduga mampu menghasilkan bakteriosin. Bakteriosin yang diproduksi oleh keenam isolat tersebut dapat terdegradasi oleh enzim Proteinase K (1 mg/ml). Isolat SKE9 teridentifikasi sebagai Lactobacillus curvatus merupakan kandidat isolat BAL terbaik karena menghasilkan  bakteriosin dan menghambat pertumbuhan Staphylococcus aureus dan Salmonella thypimurium dengan indeks penghambatan masing-masing sebesar 2,94 ± 0,59 dan 2,89 ± 0,89. Kata kunci : Bakteri asam laktat, Bakteriosin, Susu kambing Peranakan Etawa
KADAR MDA SPERMATOZOA SETELAH PROSES PEMBEKUAN Khairatul Insani Febrianti; Sri Rahayu; Agung Pramana W.M; Aris Soewondo
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : University of Brawijaya

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Abstract

Tujuan penelitian ini adalah untuk mengetahui efek lama pembekuan semen terhadap kadar MDA spermatozoa dan persentase viabilitas spermatozoa. Sampel yang digunakan berupa  semen beku sapi Limousin dengan lama pembekuan 2 bulan dan 3 bulan yang dikoleksi dari BBIB Singosari. Semen beku yang telah dithawing diencerkan dengan Phosphate Buffer Saline (PBS) kemudian disentrifugasi untuk memisahkan spermatozoa dan seminal plasma. Kadar MDA spermatozoa diukur dengan menggunakan uji TBA dan spektrofotometer. Pengamatan viabilitas spermatozoa dilakukan dengan menggunakan pewarnaan eosin-negrosin. Data berupa kadar MDA spermatozoa dan persentase viabilitas dianalisis dengan uji-t berpasangan dan korelasi pearson menggunakan spss 16.0. Hasil analisis menunjukkan bahwa kadar MDA spermatozoa pada lama pembekuan 2 bulan (0,0777±0,008 nM) dan 3 bulan (0,0919±0,016 nM) tidak berbeda nyata (p<0,05). Terdapat korelasi negatif antara persentase viabilitas dan kadar MDA pada spermatozoa. Kata kunci: MDA, semen beku, spermatozoa 
Etnobotani Tradisi Syariat di Kampung Adat Urug, Desa Urug, Kecamatan Sukajaya, Kabupaten Bogor Muhammad Qais Izzuddin; Rodliyati Azrianingsih
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : University of Brawijaya

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Abstract

Penelitian ini bertujuan untuk mengetahui proses tradisi syariat dan pemanfaatan tumbuhan terkait tradisi syariat. Penelitian dilakukan pada bulan Nopember 2013 sampai Mei 2014. Metode etnobotani yang digunakan yaitu metode survei, dibagi menjadi wawancara (semiterstruktur) dan observasi. Penentuan responden menggunakan snowball sampling dengan informan kunci Kepala Adat Urug Pusat dan Tengah. Observasi dilakukan dengan pengamatan langsung kegiatan syariat di rumah Kepala Adat Urug. Data disampaikan secara kualitatif dalam bentuk deskripsi. Kegiatan syariat menggunakan media khusus berupa panglay (Zingiber cassumunar Roxb.) atau air putih. Kegiatan ini diawali dengan peminta doa menyampaikan maksud/tujuan kepada Kepala Adat. Kepala Adat memanjatkan doa kepada Allah SWT berdasarkan tujuan peminta doa menggunakan media panglay atau air putih, kemudian diakhiri dengan mendekatkan media tersebut ke kepala dan dua sisi dada beliau, dan meludahinya. Setelah itu, Kepala Adat menjelaskan tata cara penggunaan dan penyimpanan media tersebut berdasarkan tata cara Kampung Adat Urug. Penggunaan media panglay dengan cara dihisap sebelum dan sesudah melakukan tujuan. Penggunaan media air putih dengan cara diminum sebelum melakukan tujuan. Penggunaan rimpang panglay untuk syariat akan mempengaruhi kesehatan seperti antiinflamasi (antiradang), analgesik dan chondroprotektif. Tradisi syariat secara aspek konservasi menjaga eksistensi panglay melalui penanaman tumbuhan tersebut di pekarangan warga. Kata kunci: air, Allah SWT, kesehatan, kristal, panglay.
Effect of Elephantopus scaber.L and Polyscias obtusa Leaf Extract for CD8+ and CD8+CD62L+ T Cell Modulation in Balb/c Mice Nurul Faizah; Muhammad Sasmito Djati
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : University of Brawijaya

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Abstract

Medicinal herbal is highly recommended especially for pregnant women with bacterial infection to reduce the consumption of synthetic antibiotics that are harmful to their body and also fetus. E. scaber and P. obtusa are believed to increase the number of imunocompetent cells such as CD8+ and CD8+CD62L+ T cell cause both plants have biochemical compound such as saponin and flavonoid. These plants good for immune system, but optimum dose of mixture extracts from both leaves to increase the number of CD8+ and CD8+CD62L+ T cell in mice is doesnt know yet. This study to determine effect of E. scaber and P. obtusa leaf extract by compared the number of CD8+ and CD8+CD62L+ T cell between control mice and treated mice. E. scaber and P. obtusa leaf extract can not reduce relative number of CD8+ T cells compared with control mice after 14 days treatment and 18 days. Even it show little different number but it was not significantly different, whereas the number of CD8+ decreased in K2 control mice also not significantly different than other treatments. K1 group is always show the highest number of CD8+ T cell compared to other treatments. CD8+ was decreased by increasing the doses of P. obtusa leaf extract. Key word: E. scaber, CD8+ T cell, CD8+CD62L+ T cell, P. obtusa, Salmonella typhimurium.
Efektivitas Pemberian Ekstrak Ethanol Daun Polyscias obtusa dan Elephantopus scaber terhadap Modulasi Sel T CD4+ dan CD8+ pada Mencit Bunting BALB/c Roffico Roffico; Muhammad Sasmito Djati
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : University of Brawijaya

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Abstract

ABSTRAK Tanaman yang memiliki potensi untuk digunakan sebagai obat dalam pengobatan tradisional adalah Kedondong Laut (Polyscias obtusa) dan Tapak Liman (Elephantopus scaber. L). Tanaman ini mengandung senyawa aktif yang dapat mempengaruhi mekanisme pertahanan tubuh. Penelitian ini bertujuan untuk mengetahui bagaimana efek dari ekstrak ethanol daun Polyscias obtusa dan Elephantopus scaber. L terhadap ekspresi sel T CD4+ dan CD8+ pada mencit bunting BALB/c. Hasil menunjukkan bahwa jumlah relatif sel T CD4+ dan CD8+ tidak berbeda nyata (p> 0,05) dapat diketahui dari peningkatan dan penurunan yang terjadi pada setiap perlakuan dibandingkan dengan kontrol. Hal ini berarti, rata-rata jumlah relatif sel T tidak berbeda secara nyata untuk perlakuan yang diberikan pada mencit bunting BALB/c. Berdasarkan hasil output Tukey Test dan subset yang terbentuk terlihat bahwa jumlah relatif sel T tidak berbeda nyata untuk perlakuan yang diberikan pada mencit bunting BALB/c. Hasil juga menunjukkan jumlah relatif sel T memang berbeda secara nyata (p< 0,05) untuk waktu pembedahan. Namun, setelah dilakukan Tukey Test subset yang terbentuk menunjukkan bahwa jumlah relatif sel T tidak berbeda nyata (p> 0,05) terhadap waktu pembedahan. Kenaikan dan penurunan jumlah sel T CD4+ dan CD8+, kemungkinan karena aktivitas biologis senyawa yang terkandung dalam P. obtusa yaitu panaxidol dan stiqmasterol dalam E. scaber yang dapat bertindak sebagai imunosupresan dan imunomodulasi. Dosis optimum ekstrak ethanol daun P. obtusa dan E. scaber dalam peningkatan sel limfosit belum dapat ditentukan. Kata kunci: Elephantopus scaber, limfosit, Polyscias obtusa
Effect of Iodine Solution Treatment With Different Dosage And Exposed Duration to Metamorphosis Rate and Survival of Bullfrog Tadpoles (Rana Catesbeiana Shaw) Lutviyana Rahman; Nia Kurniawan
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : University of Brawijaya

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Abstract

Objective of this research was to know the role of iodine with different doses and exposed duration to against the rate metamorphosis and survival of bullfrog’s tadpoles. Methods on this research are acclimatization of bullfrog’s tadpoles, given iodine solution such as doses 3 mg/L, 4 mg/L, 5 mg/L and control (0 mg/L). It observe on 7th, 14th and 21st day of to exposed. The parameters that observed there are tadpole metamorphosis, survival rate and water quality. Data were analyzed using two-way ANOVA and continued with Tukey test. The results showed that water quality on a  good category for bullfrog of natural environment. ANOVA for bullfrog metamorphosis rate obtained at p-value (< 0.05). Duration of exposed and doses are significantly influent to against bullfrog’s tadpoles metamorphosis. Although survival rate of bullfrog tadpoles was decline at 21th duration and survival rate of bullfrog’s lowest in doses 5 mg/L. The conclusion is treatment of iodine solution at given in the medium of life bullfrog’s tadpoles and exposed duration of iodine can increase the rate of metamorphosis, but inclined decrease survival rate of bullfrog’s tadpoles. Iodine solution most effect on the rate metamorphosis bullfrog’s tadpoles on exposed 7th, 14th and 21st days on the doses of iodine solution 5 mg/L (5 ppm), while survival rate of bullfrog’s tadpoles is the highest on treatment control (0 mg/L).
Single Cell Protein Production using Yeast Isolates from Soy Factory Waste by Co-culture Method Chintya Corin; Tri Ardyati
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : University of Brawijaya

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Abstract

Nutrient content in soy waste able to allow growth of yeasts. Yeast isolates have potency to be used in the production of single cell protein. The research was carried out to study isolates for production of single cell protein using co-culture method and to determine the effect of co-culture method in protein content obtained. The isolates used were I2YP5K1, I2YP5K2, KYP6K1, KYP3K2, AYP6K1, AYP6K2, and AYP5K4. Steps used in this research were grown the yeast isolates in Yeast Malt Broth, assay of antagonist among yeast isolates, single cell protein production, and identification using API 20C AUX. Parameters measured were biomass, number of cells, and protein content. Design of research using two-way ANOVA with three replications. Isolates able to be used for co-culture method in the production of single cell protein were KYP3K2 (Saccharomyces cereviseae), AYP6K1, AYP6K2, and AYP5K4. Co-culture methods does not increase biomass and number of cells, however able to increase the protein content. Protein production by co-culture isolates KYP3K2 and AYP6K2 is highest than other co-culture isolates with protein content 1.31 mg / g.   Key words : Co-culture, single cell protein, soy waste, yeast.
Analisis Polimorfisme Gen BMP-15(Bone Morphogenetic Protein-15) dengan Metode PCR-RFLP dan Hubungannya dengan Performan Reproduksi Sapi Friesian Holstein (FH) Lutfiatun Izzatul Lailiya; Sri Rahayu
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : University of Brawijaya

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Abstract

Penelitian ini bertujuan untuk mengetahui adanya polimorfisme gen BMP-15 (Bone morphogenetic protein-15) dengan menggunakan teknik PCR-RFLP dan hubungannya dengan performan reproduksi sapi Friesian Holstein (FH). Sampel yang digunakan dalam penelitian ini adalah 10 sapi FH betina yang diambil dari UPT. Balai Besar  Pelatihan Peternakan, Songgoriti, Batu. Isolasi DNA dari darah dengan menggunakan metode salting out. Amplifikasi gen BMP-15 dilakukan dengan teknik PCR menggunakan primer forward 5’GCTCTGGAATCACAAGGGG3’ dan Primer reverse 5’AGAGATGGGGAGCGAT GAT 3’. Polimorfisme gen BMP-15 dianalisis dengan teknik PCR-RFLP menggunakan enzim restriksi HaeIII. Amplifikasi gen BMP-15 menghasilkan amplikon dengan panjang 360bp. Analisis PCR-RFLP menghasilkan dua haplotip yang menunjukkan adanya polimorfisme. Haplotip I memiliki ukuran fragmen DNA 90bp dan 190bp. Haplotip II memiliki ukuran fragmen 90bp, 190bp, 290bp, dan 350bp. Data performan reproduksi 10 sapi fertil yang digunakan adalah 3 sapi bunting dan 7 sapi memiliki data  calving interval pada range 13-16 bulan, days open pada range 3-7 bulan dan 2 sapi beranak kembar. Berdasarkan data yang ada, diketahui bahwa sapi yang termasuk dalam haplotip II memiliki data performan reproduksi yang bervariasi. Berdasarkan hasil penelitian dapat disimpulkan bahwa polimorfisme gen BMP-15 yang ada, tidak menunjukkan adanya hubungan dengan performan reproduksi sapi FH.   Kata Kunci : Gen BMP-15, Performan reproduksi,  Polimorfisme

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