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Dinia R Dwijayanti,
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Biotropika
Published by Universitas Brawijaya
ISSN : 23027282     EISSN : 25498703     DOI : 10.21776/ub.biotropika.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biotropika: Journal of Tropical Biology invites research articles, short communication, and reviews describing new findings/phenomena of biological sciences in tropical regions, specifically in the following subjects, but not limited to biotechnology, biodiversity, microbiology, botany, zoology, biosystematics, ecology, and environmental sciences.
Arjuna Subject : -
Articles 4 Documents
 1 
Search results for , issue "Vol. 12 No. 3 (2024)" : 4 Documents clear
Digestive Enzymatic Activity Profiles and Larval Growth Performance of Barramundi, Lates calcarifer (Bloch, 1790), From Indonesian and Australian Broodstocks Wibawa, Gigih Setia; Haryanti, Haryanti; Kurniawan, Nia
Biotropika: Journal of Tropical Biology Vol. 12 No. 3 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.03.02

Abstract

Barramundi fish is one of the most important aquaculture commodities, with cultivation challenges closely related to its survival and growth. There is still a lack of information on digestive enzymatic activity profiles and larval growth, and the use of Australian brooders rather than Indonesian brooders is still frequent. This study aims to investigate digestive enzymatic activity profiles and their correlation to the growth performance of barramundi larvae, Lates calcarifer (Bloch, 1790), originating from Indonesia and Australia. The digestive enzyme activity profile was evaluated using real-time quantitative polymerase chain reaction (RT-qPCR). First, larvae were reared in captivity till day 20 (20 dah). During this rearing, measurements of length and weight growth were done every day. Experiment data shows Australian fish larvae were greater than that of the Indonesian. Their digestive enzyme activity also confirmed that Australian larvae had more activity than Indonesian larvae.
Arthropod Community Structure as an Indicator of Land Quality for Coffee Agroforestry Rehabilitation in Kopen Dukuh, Glagah, Banyuwangi Regency Naim, Abu; Tsamrotul, Yayu; Dwi N, Lulut
Biotropika: Journal of Tropical Biology Vol. 12 No. 3 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.03.04

Abstract

Kopen Dukuh, a village in Banyuwangi District, was previously known for its rich biodiversity. However, human activities, including plant exploitation and stone quarrying, have caused significant environmental damage. To address this issue, restoration efforts through agroforestry planting were implemented to improve the ecosystem and meet community needs. Monitoring the success of restoration was conducted by observing arthropods using three methods: the Berlese-Tullgren method for soil arthropods at a depth of 10 cm, pitfall traps for ground-surface arthropods, and aerial traps for airborne arthropods. Observations were distributed across 20 plots: 10 in restored areas (R) and 10 in un-restored areas (U). The results identified 41 arthropod families, with 34 families found in restored areas and 22 families in un-restored areas. The diversity index (H') indicated that restored areas had a relatively high diversity (H': 2.22, dominance value S: 0.8), while un-restored areas had a moderate diversity (H': 2.00, dominance value S: 0.87). Restored areas also showed improved environmental parameters, including higher soil moisture (85%), optimal soil temperature (22.81°C), a near-neutral pH (6.12), and increased organic carbon content (7.05%). In contrast, un-restored areas had lower soil moisture (78%), similar soil temperature (22.78°C), more acidic pH (4.8), and slightly lower organic carbon content (6.79%). The QBS index, which uses arthropods as bioindicators, further supported these findings. Restored areas exhibited higher QBS values (30–46) compared to un-restored areas (26–32), indicating better soil biological quality. These results demonstrate that agroforestry restoration plays a crucial role in improving both biotic and abiotic soil quality, contributing significantly to ecosystem recovery.
Herpetofauna Diversity and Conservation Value in The Mountain Ecosystems of Gunung Sigogor and Gunung Picis Nature Reserve, East Java, Indonesia Muammar Kadafi, Ahmad; Fatiqin, Awalul; Priambodo, Bagus; Firmansyah, Richo; Dwi Nur Aji, Fajar; Wahyu Widodo, Tri; Gunawan; Permana, Danafia; Yanuar Adiba, Faisal; Ristanto, Yuri
Biotropika: Journal of Tropical Biology Vol. 12 No. 3 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.03.03

Abstract

Gunung Picis and Gunung Sigogor nature reserves, located in the Wilis mountain range of East Java, were established in 1924 and 1936, respectively. These areas are critical for biodiversity preservation as mandated by Indonesia's Law No. 5 of 1990 on Conservation of Biological Resources and Ecosystems. Despite previous surveys documenting various mammals, birds, reptiles, and amphibians data remain limited. Herpetofauna, particularly amphibians, are vital bio-indicators due to their sensitivity to environmental changes, but they face significant threats from habitat loss and exploitation. This study, conducted in November 2020 using Visual Encounter Surveys and opportunistic searches, aimed to catalog herpetofauna diversity in these reserves. The survey identified 29 herpetofauna species, with Nyctixalus margaritifer rediscovered at a new site. Most species were classified as Least Concern (LC), except for the Gonocephalus kuhlii (Vulnerable/VU), indicating potential habitat threats. Analysis of species distribution highlighted "Wates stream" as the most diverse habitat. These findings provide a critical foundation for future conservation efforts, emphasizing the need for targeted strategies to protect amphibian and reptile species in Gunung Picis and Gunung Sigogor nature reserve.
Identification and Genetic Diversity of Pineapple Local Accessions from Kediri and Closely Related Species (Bromeliaceae) Based on matK Gene Rasyadan Taufiq Probojati; Hadiyanti, Nugraheni; Hapsari, Lia
Biotropika: Journal of Tropical Biology Vol. 12 No. 3 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.03.01

Abstract

Ananas comosus L. belongs to the Bromeliaceae family and has around eight subfamilies. Accessions of local pineapple have spread widely in several areas, one of which is in Kediri. However, some accessions and closely related species (Bromeliaceae) likely remain poorly understood relationships. The highly diverse morphological characters and low levels of sequence divergence in Bromeliaceae have been problematic in resolving the reconstruction of phylogenetic relationships. This study aimed to evaluate the efficiency of the matK gene as a DNA barcode to determine genetic diversity and reconstruct a phylogenetic tree in local pineapple and closely related species (Bromeliaceae). A total of 15 specimens were used in this research, of which 7 were local pineapple accessions from Kediri and eight were closely related species from the Bromeliaceae family. Whole genome DNA was isolated using a Tiangen kit and amplified using the polymerase chain reaction (PCR) technique using a specific primer. The results showed that matK was easily amplified with a DNA fragment length of 750-800 bp. The average composition value of each base was 36.9% T (U) bases, 30.1% in A bases, 15.9% in G bases, and 17.1% in C. BLAST analysis of the sequences showed that all samples were confirmed and identified according to the species names spread across local pineapple production areas (with query cover of 96-98%). However, the species names registered in the PBG database were confirmed to be different species but still in the same genus, i.e., samples B9-B14. The phylogenetic relationship analysis distinguished each accession into two large clades according to sub-families, i.e., Bromelioideae and Pitcairnioideae. This research shows that the matK gene is suitable and recommended as a DNA barcode for local pineapple and closely related species.

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