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PRELIMINARY SCREENING RESISTANCE OF Musa GERMPLASMS FOR BANANA BUNCHY TOP DISEASE IN PURWODADI BOTANIC GARDEN, PASURUAN, EAST JAWA Hapsari, Lia; Masrum, Ahmad
Buletin Kebun Raya Vol 15, No 2 (2012): Buletin Kebun Raya Vol. 15 (2) July 2012
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Banana Bunchy Top Disease (BBTD) caused by Banana Bunchy Top Virus (BBTV) is one of the most serious banana diseases, constraint and devastate to the local and regional banana production. Some of banana cultivars were more readily infected by the virus, but considering no cultivars is resistant. The incidences of BBTD and the type and severity symptoms in natural conditions without any artificial inoculation were recorded. The observations results on 2009 showed that 12.14% of the total accessions indicated positively infected BBTD. The BBTD incidences were increased rapidly about twofolds per year, 28.57% in October 2011 and 54.29% in October 2011. The spread and development of BBTD is optimum at humid, high rainfall intensity and temperatures, high soil fertility and also low light intensity or shaded. The latest monitoring on February 2012 (62.14%) comprises of 64 accessions were positively infected and 39 accessions were symptomless. Those symptomless accessions were initially indicated as tolerant banana cultivars to BBTD e.g. Pisang Kepok (BBB), Pisang Sobo (BBB), Pisang Bandung (ABB), Pisang Nangka (AAB), Pisang Candi (AAB) and Â Pisang Raja Marto (AAB). These preliminary results are in agreement with several previous studies indicated that genotypes with one or two B genomes tend to be more tolerant to BBTD. However several cultivars which known tend to BBTD tolerant are indicated susceptible to Blood Disease. Some cultivars also showing complication symptoms both BBTD and Blood Disease.

DIVERSITY AND UTILIZATION OF Dioscorea spp. TUBER AS ALTERNATIVE FOOD SOURCE IN NGANJUK REGENCY, EAST JAVA Trimanto, Trimanto; Hapsari, Lia
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i2.504

Dioscorea spp. is local tuberous food crop that has potential as alternative food source to support food security program in Indonesia. Exploration study and collecting mission subjected to Dioscorea spp. have been conducted in Nganjuk Regency. The study was aimed to 1) determine the distribution of Dioscorea spp. in Nganjuk, 2) to characterize tuber’s morphological characteristic, and 3) to know how tuber’s utilization by local peoples. The results showed that there were 4 species of Dioscorea found in Nganjuk. They are Dioscorea alata L, Dioscorea esculenta (Lour.) Burkill, Dioscorea hispida Dennst and Dioscorea bulbifera L. Dioscorea alata (uwi) is the most cultivated by farmers in Nganjuk. It has the largest cultivar numbers which consists of 11 cultivars. Dioscorea esculenta (gembili) consists of 3 cultivars and Dioscorea hispida (gadung) consists of 4 cultivars, whereas Dioscorea bulbifera (uwi gantung) consists only 1 cultivar in one location. Farmers and local peoples play important role in conservation of Dioscorea in Nganjuk region by cultivating and utilizing it as alternative food source for home consumption.

Adaptasi Ekofisiologi Terhadap Iklim Tropis Kering: Studi Anatomi Daun Sepuluh Jenis Tumbuhan Berkayu Rindyastuti, Ridesti; Hapsari, Lia
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTStudy on foliar anatomic structure of tropical plants are useful to study various aspects of plant life related to photosynthetic, productivity, carbon sequestration, adaptation to environmental stresses as the results of global climate change, etc. This study was conducted to investigate foliar anatomic structure of ten tropical woody plant species including Barringtonia asiatica, Dracontomelon dao, Heritiera littoralis, Diospyros discolor, Calophyllum inophyllum, Antidesma bunius, Schleichera oleosa, Syzygium cumini, Madhuca longifolia dan Adenanthera pavonina; also to discuss its implication to ecophysiological adaptation to tropical dry climate. The results showed that ten woody plant species studied had variation of adaptation pattern to dry tropical climate, exposure to high light intensity or open shade and photosynthesis activity. Plant species in Group II (Antidesma bunius, Madhuca longifolia and Adenanthera pavonina) has relatively higher adaptation compared to Group I (Diospyros discolor, Calophylum inophyllum, Dracontomelon dao, Schleichera oleosa, Heritiera littoralis, Baringtonia asiatica and Syzygium cumini). Some foliar anatomic structures including palisade shape and size, stomatal index, stomatal density, spongy mesophyll thickness, the ratio of P/T and P/S are important characters that can be used as indicator of changes in environmental factors; particularly the density of stomata as indicators of climate change in dry tropic area.Keywords: adaptation, dry climate, eco-physiology, foliar anatomy, woody plant

KARAKTERISTIK FRIES UWI PUTIH (DIOSCOREA ALATA) DENGANKAJIAN KONSENTRASI KALSIUM KLORIDA DAN LAMA BLANCHING Munawaroh, Putri Syepty SriJanatul; Putri, Widya Dwi Rukmi; Hapsari, Lia
Jurnal Teknologi Pertanian Vol 19, No 1 (2018)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

ABSTRAKIndonesia memiliki jenis umbi-umbian yang belum dimanfaatkan secara optimal seperti uwi putih (Dioscorea alata). Uwi putih berpotensi dikembangkan menjadi produk makanan ringan french fries. Namun permasalahannya uwi menghasilkan tekstur yang kurang renyah dan mengalami perubahan warna karena proses pencoklatan. Tujuan penelitian ini adalah mengetahui pengaruh kombinasi perlakuan konsentrasi kalsium klorida (CaCl2) dan lama blanching terhadap sifat kimia, fisik dan organoleptik fries uwi putih (Dioscorea alata). Metode penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK) yang terdiri dari 2 faktor yaitu konsentrasi kalsium klorida (CaCl2) (0, 1, dan 2%) dan lama blanching (3, 6, dan 9 menit). Data dianalisis menggunakan metode analisis ragam (ANOVA) dan dilanjutkan dengan uji lanjut BNT 5%. Pemilihan perlakuan terbaik menggunakan metode Zeleny. Perlakuan terbaik diperoleh pada kombinasi perlakuan konsentrasi kalsium klorida (CaCl2) 2% dan lama blanching 6 menit dengan kadar air 40.76%; kadar pati 26.14%; kadar lemak 19.62%; kadar protein 2.78%; kadar abu 1.09%; tekstur 5.97 N; kecerahan (L*) 63.7; kemerahan (a*) 1.34; kekuningan (b*) 15.41; nilai organoleptik rasa 2.77; aroma 3.5; warna 3.43; tekstur 2.93; dan kenampakan 3.17Â ABSTRACTIndonesia has many kind of tubers that did not use optimally such as water yam (Dioscorea alata). Water yam can potentially developed into a product such as french fries. However, the problem of water yam has a less crispiness texture and has a colour distortion caused by browning process. The aims of this research was to determine the effect of calcium chloride concentrations (CaCl2) and blanching time on the chemical, physical, and organoleptic characteristics of water yam (Dioscorea alata) fries. The research used a Randomized Block Design (RDB) with 2 factors that were concentration of calcium chloride (CaCl2) (0, 1, and 2%) and blanching time (3, 6, and 9 minutes). Data were analyzed using ANOVA, followed by LSD with significant level 5%. The determination of the best treatment using multiple attribute Zeleny. The best treatment was concentration of calcium chloride (CaCl2) 2% and theblanching time of 6 minutes with water content 40.76%; starch 26.14%; fat 19.62%; protein 2.78%; ash 1.09%; texture 5.97 N; lightness (L *) 63.7; redness (a *) 1.34; yellowness (b *) 15.41; the value of the organoleptic taste 2.77; aroma 3.5; color 3.43; texture 2.93; and appearance of 3.17

FRUIT CHARACTERISTIC AND NUTRIENT VALUES OF FOUR INDONESIAN BANANA CULTIVARS (Musa spp.) AT DIFFERENT GENOMIC GROUPS Hapsari, Lia; Lestari, Dewi Ayu
AGRIVITA, Journal of Agricultural Science Vol 38, No 3 (2016): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i3.696

Aims of this research were to describe the morphology and analyze nutrient values of mature fruits at three different genomic groups of Indonesian banana cultivars including Pisang Berlin (AA), Ambon Hijau (AAA), Raja Bandung (ABB) and Kepok (ABB). Fruit characterization results show that each banana cultivar had specific characteristics related to their genomic group. Pisang Berlin has bright yellow peel and pulp, sugary taste. Pisang Ambon Hijau has fine curved fruit shape, sweet taste and aromatic. Pisang Raja Bandung has medium thickness and yellow peel, firm flesh, sweet and slightly acidic taste. Pisang Kepok has thick coarse and yellow peel with dark brown blotches, mild sweet taste. Fruit characters of Pisang Berlin and Ambon Hijau are close related to their ancestral parentsâ€™ Musa acuminata wild species, whereas Pisang Kepok and Pisang Raja Bandung as hybrid cultivars have intermediate characters between Musa acuminata and Musa balbisiana wild species. Nutrient analysis revealed that mature banana pulp contain of high carbo-hydrates (16.72-35.24 g 100g-1), total sugar (12.12-20.82 g 100g-1), vitamin C (16.45-30.27 g 100g-1) and potassium (275-375 g 100g-1); moderate protein (1.48-1.78 g 100g-1) and low fat (0.03-0.08 g 100g-1). About 100 g edible portion of banana fruit produce 73.43 to 148.80 calories.Â

ADAPTASI EKOFISIOLOGI TERHADAP IKLIM TROPIS KERING: STUDI ANATOMI DAUN SEPULUH JENIS TUMBUHAN BERKAYU Rindyastuti, Ridesti; Hapsari, Lia
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3089

ABSTRACTStudy on foliar anatomic structure of tropical plants are useful to study various aspects of plant life related to photosynthetic, productivity, carbon sequestration, adaptation to environmental stresses as the results of global climate change, etc. This study was conducted to investigate foliar anatomic structure of ten tropical woody plant species including Barringtonia asiatica, Dracontomelon dao, Heritiera littoralis, Diospyros discolor, Calophyllum inophyllum, Antidesma bunius, Schleichera oleosa, Syzygium cumini, Madhuca longifolia dan Adenanthera pavonina; also to discuss its implication to ecophysiological adaptation to tropical dry climate. The results showed that ten woody plant species studied had variation of adaptation pattern to dry tropical climate, exposure to high light intensity or open shade and photosynthesis activity. Plant species in Group II (Antidesma bunius, Madhuca longifolia and Adenanthera pavonina) has relatively higher adaptation compared to Group I (Diospyros discolor, Calophylum inophyllum, Dracontomelon dao, Schleichera oleosa, Heritiera littoralis, Baringtonia asiatica and Syzygium cumini). Some foliar anatomic structures including palisade shape and size, stomatal index, stomatal density, spongy mesophyll thickness, the ratio of P/T and P/S are important characters that can be used as indicator of changes in environmental factors; particularly the density of stomata as indicators of climate change in dry tropic area.Keywords: adaptation, dry climate, eco-physiology, foliar anatomy, woody plant

Biochemical Composition and Nutritional Value of Fresh Tuber of Water Yam (Dioscorea alata L.) Local Accessions from East Java, Indonesia Fauziah, Fauziah; Mas'udah, Shofiyatul; Hapsari, Lia; Nurfadilah, Siti
AGRIVITA, Journal of Agricultural Science Vol 42, No 2 (2020)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v0i0.2552

Water yam (Dioscorea alata L.) is an important tuber crop containing essential nutrition as an alternative food source. This study aimed to analyze the biochemical composition and nutritional value of fresh tuber of fifteen local accessions of water yam from East Java and followed by the accession selection for the recommendation of accession with the best nutritional value. Results showed that the nutritional value of proximates including moisture, carbohydrate and fiber were significantly different; except in protein, fat, and ash, with the range of moisture (65.47-82.46%), carbohydrate (17.10-29.37%), protein (1.29- 3.00%), fat (0.00-0.29%), fiber (6.70-11.62%) and ash (0.85-1.44%). The ranges of mineral contents (mg/100g) were K (224.54-483.21), Ca (15.63-61.97), Mg (16.75-43.06), Fe (1.40-13.40), Zn (0.43-2.83) and P (329.37-699.91); Na mostly not detected. The anti-nutritions contents (mg/100g) comprised tannin (63.36-167.68) and oxalic acid (12.73-44.92). Analysis through HCA showed three clusters with a range of similarity from 67% to 96%; through PCA scatter biplot clearly illustrated the variation and correlation pattern among local accessions. Six promising accessions are recommended for further breeding and development i.e. Uwi Perti/30, Uwi Bangkulit/36, Uwi Bangkulit/42, Uwi Bangkulit/43, Uwi Biru/58 and Uwi Legi/66, based on their highest carbohydrates and protein content, and the lowest fat content.

Wild Musa Species Collection of Purwodadi Botanic Garden: Inventory and Its Morpho-taxonomic Review Hapsari, Lia
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Indonesia, being part of the center of origin of bananas (Musaceae), has a large number diversity of bananas both wild seeded species and edible seedless cultivated varieties. Inventory of wild Musa species in Purwodadi Botanic Garden has been conducted through compiling data records from PBGs Registration section, field inspection and observation to living collections in the garden, herbarium specimens and literature studies. The results show that total 17 wild Musa accessions has been recorded planted in Purwodadi Botanic Garden since 1990 until 2012; comprises of 8 Musa acuminata sub species, 2 Musa balbisiana forms, 1 Musa ornata, 1 Musa troglodytarum, 1 Musa borneensis and 4 unidentified species Musa spp.; but only 8 living accessions remained in 2012. Morpho-taxonomic review of those 8 wild Musa accessions remained will be discussed in this paper including their geographical distributions. According to its differentiated morphological characteristics observations, it is known that there are three accessions were resembled cultivars and one unidentified species have been determined its species level, so that their registration identity needs to be revised. It is important next to prioritize ex-situ conservation of wild Musa species not yet collected in Purwodadi Botanic Garden especially from Eastern Indonesia.

Variasi Genetik dan Filogeografi Pisang Raja (Musa spp.) di Pulau Jawa Berdasarkan Sekuen Internal Transcribed Spacer Wahyudi, Didik; Gusmiati, Lutfiana Hasanah; Hapsari, Lia
Jurnal Biotek Vol 11 No 2 (2023): DESEMBER
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v11i2.40807

Pisang Raja is an important banana cultivar for the Javanese people since ancient times, with many morphological variations and local names. This study aims to determine the genetic variation and phylogeography of Pisang Raja based on Internal Transcribed Spacer (ITS) sequences. The results of the research can be used as a reference for developing superior Pisang Raja cultivars that are resistant to disease and make farmers easier to choose Pisang Raja cultivars that suit with geographical conditions on Java Island. This study used 9 samples of Pisang Raja (in-group) and 2 samples of wild bananas (out-group). The research method started with DNA isolation, qualitative and quantitative tests, ITSL and ITS4 primer amplification, and sequencing. Data analysis performed included polymorphism, phylogenetics (MP, ML, NJ and BI) and haplotype. The results showed that the ITS sequences in Pisang Raja was amplified with a length of 600-700 bp and GC content of 61.70%. Polymorphism analysis showed that the genetic variation of Pisang Raja in Java was very high. Phylogenetic analysis resulted in 4 phylogenetic trees with relatively same topologies, where the in-groups were divided into 2 clades, namely clade I (AAA and AAB) and clade II (AAA and ABB). The highest genetic similarity was showed by Raja Brentel and Raja Bali (89.43%) and the least was Raja Gareng and Raja Seribu (56.82%). Haplotype analysis formed 11 haplotypes which grouped into 4 groups. Pisang Raja evolution comes from two ancestors, i.e. M. acuminata and M. balbisiana. The phylogeography of ITS gene flow in Pisang Raja originates from Central Java then spread to East Java and Jakarta. This is presumably due to the high utilization followed by the planting of Pisang Raja by the people of Central Java for ritual and ceremonial events in the past which influenced the domestication process. The results of this study can served as the reference for genetic conservation strategy of Pisang Raja.

Characterization and impact of planthopper (Sanurus indecora) infestation on kepel (Stelechocarpus burahol) plants Saputra, Yudha; Anindita, Devina Cinantya; Hadiyanti, Nugraheni; Supandji, Supandji; Hapsari, Lia
Jurnal Ilmiah Pertanian Vol. 21 No. 3 (2024): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v21i3.19003

Kepel (Stelechocarpus burahol), designated as the floral identity of the Special Region of Yogyakarta, Indonesia, faces challenges from plant pests that threaten its sustainability. Understanding the morphology and impact of pests such as planthoppers on kepel is crucial for developing effective pest management strategies. Despite its significance, there is a gap in research regarding the specific pests affecting kepel and their impact. This study aims to identify the morphological characteristics of planthopper pests and examine their infestation levels on kepel plants. Using a qualitative method with purposive sampling, samples were collected from kepel plants identified as infected by tree planthoppers. Morphological observations identified the pest species as Sanurus indecora from the Flatidae family. Sampling from two infected plants resulted in 81 nymphs and 16 adult S. indecora. Infestation analysis showed that kepel plant XII.G.D had a 7.4% infestation rate, while XIV.G.II had a 23% infestation rate. These findings highlight the presence and impact of S. indecora on kepel plants, providing critical information for the bioecology of this pest. This study not only fills a research gap by documenting the pests affecting kepel but also contributes to the broader knowledge required for developing targeted pest management strategies, ensuring the conservation and health of kepel plants in Yogyakarta, Indonesia.

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