cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Current Biochemictry
Contact Email
current.biochemistry@gmail.com
Phone
-
Journal Mail Official
imart171@yahoo.com
Editorial Address
-
Location
Kota bogor,
Jawa barat
INDONESIA
Current Biochemistry
Published by Institut Pertanian Bogor
ISSN : 23557877     EISSN : 23557931     DOI : -
Core Subject : Science, Education,
Biochemistry, Genetics & Molecular Biology Education
Current Biochemistry (CB) publishes the results of original research that contribute significantly to the understanding of the chemical compound and reaction that occur within living organism. Preference will be accorded to manuscripts that develop new concepts or experimantal approaches, particularly in the advancing areas of biochemistry science. Manuscripts that are primarily theoretical in nature or in the field of bioinformatics must be directed toward explaining important results previously not understood, making important predictions that can be experimentally tested, or developing segnificant advances in theory of general interest to biochemists. Submission of manuscripts in emerging areas in biochemistry, chemical biology, biophysics, proteomics, model studies and structures, cellular and molecular biology, computational biochemistry, biotechnology, and new methods development is encouraged especially if they address basic biochemical mechanisms.
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol. 3 No. 1 (2016)" : 5 Documents clear
Deodorization of Latex Waste and Decolorization of Textile-Coloring Agent by Omphalina sp. using Batch and Continuous Methods Erna Puspasari; I Made Artika; Tri Panji
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Generally industrial waste water is a pollutant to environment as it produces strong odor and color. Omphalina sp is one of white rot fungi that can be used as an odor and color effluent reducer. Omphalina sp has laccase enzyme that plays role in deodorization and decolorization. The aim of this research was to determine the best method in deodorization and decolorization among batch and continous (pack bed flow, biotray, and rotary contactor) methods. The results of deodorization and decolorization showed that the rotary contactor method was better than the batch, pack bed flow and biotray methods. At dye concentration of 50 ppm, after 24 hours treatment, the absorbance value for batch, pack bed flow, biotray, and rotary contactor methods was 0.520, 0.423, 0.425, and 0.357, respectively. At dye concentration of 150 ppm, after 24 hour treatment, the absorbance value for batch, pack bed flow, biotray, and rotary contactor methods was 0.709, 0.629, 0.658, and 0.592, respectively. At dye concentration of 50 ppm, percentage of dye absorption after 24 hour treatment for batch, pack bed flow, biotray, and rotary contactor methods was 20.550%, 35.447%, 35.141% and 45.531% respectively. At dye concentration of 150 ppm, percentage of dye absorption after 24 hour treatment for batch, pack bed flow, biotray, and rotary contactor methods was 7.320%, 17.843%, 13.987%, and 22.614%. The qmaks value at dye concentration of 50 ppm after 24 hour treatment for batch, pack bed flow, biotray, and rotary contactor methods was 0.020, 0.076, 0.083, and 0.124 respectively. qmaks value at dye concentration of 150 ppm after 24 hour treatmeny for batch, pack bed flow, biotray, and rotary contactor methods was 0.009, 0.077, 0.046, and 0.100 respectively. Odor scale for batch, pack bed flow, biotray, and rotary contactor methods decreased from 5 to 2.9, 1.4, 1.6 and 1.1 respectively.
Characterization and Toxicity of Temulawak Curcuminoid Nanoparticles Riki Riki; Popi Asri Kurniatin; Laksmi Ambarsari; waras Nurcholis; Latifah K Darusman
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Temulawak exctract contains curcuminoids which have anticancer potential. However, clinical application of curcuminoid has been limited due to its low bioavailability. One of the efforts that can be developed to solve this problem is incorporated curcuminoids into Solid Lipid Nanoparticles (SLN) carriers system. The objective of this study was to characterize dan evaluate anticancer potential of temulawak ethanolic fraction nanoparticles. HPLC method was used to determined curcuminoids content of temulawak ethanolic fraction. Characterization indicators like polydispersity index, particle size, morpholgy, and entrapment efficiency. HPLC chromatogram has shown of curcumin, demethoxycurcumin, and bisdemethoxycurcumin were found in temulawak ethanolic fraction. The particle size of nanoparticles obtained in this study was 648.4 ± 95 nm with polydispersity index value of 0.216. A uniform size distribution of nanoparticles as observed by Transmission Electron Microscopy (TEM). The entrapment efficiency of curcuminoid in nanoparticles was about 29.8%. Based on results of BSLT obtained temulawak extract Lethal Concentration (LC50) value of 213.24 ppm and 828.78 ppm of nanoparticles.
Biosorption Copper (Cu) and Mercury (Hg) by Omphalina sp. using Batch, Rotary, Biotray, and Pack Bed Flow Methods Desi Purwaningsih; I Made Artika; Tri Panji; Suharyanto Suharyanto
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Heavy metal waste treatment often uses dangerous chemicals. Omphalina sp is a nonpathogenic fungi that can be used to reduce the harmful effects of waste treatment. The use of fungal biomass has advantages such as low operating costs, efficient, and high metal binding capacity, minimal sludge, metals can be recovered, biosorbent can be regenerated, raw materials available easily, can use inactivated microorganism, and does not require additional nutrients. In the present study optimization of the biomass utilization for waste water treatment was conducted by comparing batch, rotary, packbed flow, and biotray methods. Results showed that Omphalina sp can reduce mercury level up to 91.38% with rotary, 83.98% with biotray, 87.14% with pack bed flow, and 31.94% with batch methods respectively from initial Hg concentration of 3 ppm. Similarly, Omphalina sp can reduce copper level up to 23.58% with rotary, 22.66% with biotray, 10.53% with pack bed flow, 10.17% with batch methods respectively from initial Cu concentration of 100 ppm. Optimum absorption Hg and Cu occurs in the first one hour.
Amplification and Analysis of Cytocrome Oxidase I of Polypedates leucomystax from Bogor Agricultural University Area Perkasa Arian; I Made Artika; Syamsul Falah
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

DNA barcoding has become a useful tool for identifying and confirming of species within a known taxonomic framework. A large-scale effort is underway to barcode all amphibian species using the universally sequenced DNA region, a partial fragment of mitochondrial cytochrome oxidase subunit I (COI). This study was aimed to use DNA barcoding technique to identify and confirm species of Polypedates leucomystax and to analyze their phylogenetic relationship. Samples of Polypedates leucomystax were collected from Campus Area of Bogor Agricultural University. The cytochrome oxidase I gene of 600-700 nucleotides were amplified and observed in agarose gel electrophoresis. Forward sequence (604 base pairs) of COI gene was used for phylogenetic analyses. BLAST analysis against BOLD System database showed 95.75% identity with sequences of Polypedates leucomystax. The pairwise genetic distances of Polypedates leucomystax with Rhacophorus schlegelii, Limnonectes fujianensis, Fejervarya cancrivora, and Bufo melanostictus were 0.274, 0.352, 0.339, 0.339, 0.393, respectively. These results illustrated that the genetic identification is congruence with the morphological identification. Phylogenetic tree analysis showed that the samples were in one clade with other tree frogs. The DNA barcoding technique based on the sequence of COI gene can therefore be used to identify and confirm species of Polypedates leucomystax.
Optimization of Formula Film based on Amylopectin Cassava Starch and Carrageenan as a Raw Materials of Capsule Shell G Jeni christi A; Laksmi Ambarsari; Heri Purwoto
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Capsules are very important in the packaging of pharmaceutical preparations. Commercial capsule shell is generally made of gelatin from cows and pigs. Alternatives to gelatin from non-animal raw materials can be obtained from polysaccharides like starch and carrageenan. The purpose of this study was to obtain the optimum formula between amylopectin and carrageenan as a raw material subtitute for gelatin capsule shell. Program Design Expert 7.0.0 (trial version) with Response Surface Methodology (RSM) Central Composite Design was used to optimize formula with three variable factors and three response variables. Based on the analysis by determining the adjusted range, program recommends 29 optimization solution with desirability value 1. Formula 6 and 28 was selected for validation with factors 1,01% of amylopectin, 1.01% of carrageenan, 2.17% of glycerin (formula 6) and 3.00% of amylopectin, 2.00% of carrageenan, 2.90% of glycerin (formula 28). Prediction response value was 12.94% of moisture content, 6.35% of ash content (formula 6) and 12.99% of moisture content, 8.67% of ash content (formula 28). Validation result value was 21.45% of moisture content, 7.58% of ash content, 6.12 minutes of solubility in water (formula 6) and 17.67% of moisture content, 7.78% of ash content, 9.30 minutes of solubility in water.

Page 1 of 1 | Total Record : 5

 1 

Filter by Year

2016 2016


Reset
Filter By Issues
All Issue Vol. 12 No. 1 (2025): Vol. 12 No. 1 (2025) Vol. 11 No. 1 (2024) Vol. 10 No. 2 (2023) Vol. 10 No. 1 (2023) Vol. 9 No. 2 (2022) Vol. 9 No. 1 (2022) Vol. 8 No. 2 (2021) Vol. 8 No. 1 (2021) Vol. 7 No. 2 (2020) Vol. 7 No. 1 (2020) Vol. 6 No. 2 (2019) Vol. 6 No. 1 (2019) Vol. 5 No. 3 (2018) Vol. 5 No. 2 (2018) Vol. 4 No. 3 (2017) Vol. 4 No. 2 (2017) Vol. 4 No. 1 (2017) Vol. 3 No. 3 (2016) Vol. 3 No. 2 (2016) Vol. 3 No. 1 (2016) Vol. 2 No. 3 (2015) Vol. 2 No. 2 (2015) Vol. 2 No. 1 (2015) Vol. 1 No. 3 (2014) Vol. 1 No. 2 (2014) Vol. 1 No. 1 (2014) More Issue