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Current Biochemistry
Published by Institut Pertanian Bogor
ISSN : 23557877     EISSN : 23557931     DOI : -
Core Subject : Science, Education,
Biochemistry, Genetics & Molecular Biology Education
Current Biochemistry (CB) publishes the results of original research that contribute significantly to the understanding of the chemical compound and reaction that occur within living organism. Preference will be accorded to manuscripts that develop new concepts or experimantal approaches, particularly in the advancing areas of biochemistry science. Manuscripts that are primarily theoretical in nature or in the field of bioinformatics must be directed toward explaining important results previously not understood, making important predictions that can be experimentally tested, or developing segnificant advances in theory of general interest to biochemists. Submission of manuscripts in emerging areas in biochemistry, chemical biology, biophysics, proteomics, model studies and structures, cellular and molecular biology, computational biochemistry, biotechnology, and new methods development is encouraged especially if they address basic biochemical mechanisms.
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Articles 5 Documents
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Search results for , issue "Vol. 3 No. 2 (2016)" : 5 Documents clear
Phylogenetic Analysis of Cytochrome Oxidase I from Buduk Toads Duttaphrynus melanostictus and Phrynoidis asper from Bogor Muhammad Dailami; I Made Artika; Mirza Dikari Kusrini; Dodi Safari
Current Biochemistry Vol. 3 No. 2 (2016)
Publisher : IPB University

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Abstract

Indonesia have high diversity of Amphibians. Amphibians have an important role in ecosystem and produce many bioactive peptides. However, the genetic information of amphibians from Indonesia is very limited, especially Duttaphrynus melanostictus and Phrynoidis asper. The aims of this study are to determine the nucleotide sequence of cytochrome oxidase I (COI) from D. melanostictus and P. asper, to analyze their genetic diversity and their phylogenetic relationship. A total 668 base pairs of COI gene fragment were successfully amplified and their nucleotide sequence determined. P. asper (5 haplotypes) samples group have high haplotype diversity compared to D. melanostictus (1 haplotype). The results of Basic Local Alignment Search Tools (BLAST) to the NCBI and BOLD database, showed 99 % - 100 % identity to sequence of D. melanostictus. For the sequence of P. asper showed 99.23 % identity to sequence P. asper in BOLD database. There was no sequence of COI gene of P. asper in NCBI database. Genetic relationship among species in family Bufonidae, indicated that D. melanostictus has closer relation to P. asper than to another species, inspite of their pharapyletic characteristic. For intern species relationship of D. melanostictus, the data showed that D. melanostictus from Bogor have closer relationship to D. melanostictus from India than D. melanostictus from China.
Antihyperglicemic Activity of Curcuma Xanthorrizha Roxb. Nanocurcuminoid Emulsion on Streptozotocin Induced Sprague-Dawley Rat Irma Rahmayani; Laksmi Ambarsari; Mega Safithri
Current Biochemistry Vol. 3 No. 2 (2016)
Publisher : IPB University

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Abstract

Curcuminoid, a natural compound isolated from Curcuma xanthorrhiza RoxB (Temulawak) has been reported to possess antioxidant, anti-inflammatory, anticancer, antimicrobial and anti-hyperglicemic properties. The curcuminoid is weakly soluble in water that restricts its bioavailability. This problem could be overcome by incorporating curcuminoid into solid lipid nanoparticles (SLN). Nanocurcuminoid was prepare by using homogenization-ultra sonication methods. Nanocurcuminoid obtained in this study was 523.5 nm in size and polydispersity index of 0.218 with entrapment efficiency of 24.2 %. Rats were made diabetic by induction of 50 mg / kg BW STZ, treated with the chosen formula (doses 5, 10, 20 mg/kg BW) orally for 15 days. Body weight and blood glucose levels were measured from day 0, 4th,7th,11th and 15th. The results showed that 15 days of daily treatment of 10 mg/kg BW nanocurcuminoid emulsion led to a reduction of blood glucose level by 30.93±14.90 % and body weight by 15.5±13.92 %. Curcuminoid formulated in solid lipid nanoparticles could suppress a decrease in body weight and lower blood glucose levels of rats.
Total Phenolic, Anticancer and Antioxidant Activity of Ethanol Extract of Piper crocatum Vahl from Pamekasan and Karang Asem Kristina Mulia; Akhmad Endang Zainal Hasan; Suryani Suryani
Current Biochemistry Vol. 3 No. 2 (2016)
Publisher : IPB University

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Abstract

Cancer is a deadly disease that ranks the second after heart disease. One of the causes of cancer is free radicals causing oxidative stress in humans. Antioxidant is compound that can reduce oxidative stress. The antioxidant activity is closely connected with anticancer activity. It is known that in addition to being an analgesic, aphrodisiac of Java chili also has potential as anticancer. This study examined the potential anticancer and antioxidant from the ethanol extract of Java chili from Pamekasan and Karang Asem. The method used in this study began with the extraction of Java chili using ethanol 70 %, phytochemical testing, determination of total phenol anticancer testing using MTT method on MCF-7 breast cancer cell line and antioxidant testing using DPPH. This study uses Java chili extract derived from Pamekasan and Karang Asem. IC50 value of the cytotoxic activity originating from the area Karang Asem amounted to 63.283 ppm and Pamekasan of 83.662 ppm. IC50 value of antioxidants samples derived from Karang Asem area of 285.613 ppm and are derived from Pamekasan amounted to 288.037 ppm.
Application of Liquid Organic Fertilizer (Bio-fertilizer) Enriched Consortium Bacteria and Golden Snail (Pamoacea canliculata) in Ciherang Rice Flowering Agus Setiawan; Maria Bintang; Syamsul Falah
Current Biochemistry Vol. 3 No. 2 (2016)
Publisher : IPB University

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Abstract

Application of organic fertilizer today has become a necessity, benefits can be felt by farmers using organic fertilizers because increasing content of organic matter and soil nutrition so that increased productivity of agricultural land. Golden snail (Pomacea canaliculata) was introduced to Asia in the 1980s from South America as a potential food for humans. nutritional content of golden snails when added to other ingredients can be a good growth medium for growth of various types of bacteria (consortium) as basic ingredients of liquid organic fertilizer (Bio-fertilizer). Bio-fertilizer was made from golden snail plus consortium bacteria which then carried out isolation and calculation number of bacterial colonies that grow, test activity of phosphate solvent, nitrogen fixation and effectiveness of Ciherang rice flowering. Bio-fertilizer applications made in combination with solid organic fertilizers significantly increase plant height, number of tillers, multiply and accelerate flowering of Ciherang rice.
Antimicrobial activity and identification of bioactive compounds of Söfö-söfö (Acmella cf) leaf extract using GC-MS Maria bintang; Faoziduhu Bawamenewi; Mega Safithri; Fachriyan H Pasaribu
Current Biochemistry Vol. 3 No. 2 (2016)
Publisher : IPB University

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Abstract

Söfö-söfö is a traditional medicinal plant from Nias Island that can cure fever, cough, diarrhea and fungal infections on the skin. However, the scientific basis of these plants is unknown. The aim of this research was to extract the Söfö-söfö leaf by maceration method using two solvents that were 70 % ethanol and ethyl acetate, to test the antimicrobial activity of the extract by Agar diffusion method against Staphylococcus aureus, Eschercia coli, and Candida albican, and analyze secondary metabolite compounds by fitochemistry test and to determine the components of bioactive compounds by GC-MS. The results showed that the best solvent for making Söfö-söfö extract as antimicrobial is ethyl acetate with a minimum inhibitory concentration of 4000 ppm to S. aureus (1.25 ± 0.35 mm), E. coli (1 mm) and C. albican 6000 ppm (1.5 mm). The secondary metabolite compounds of ethyl acetate extract were alkaloids, flavonoids and steroids. Bioactive compounds found in the Söfö-söfö ethyl acetate extract with potential antimicrobial activity were hexadecanoic acid, stigmasterol, neophytadiene, methyl ester, squalene and phytol.

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