cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Current Biochemictry
Contact Email
current.biochemistry@gmail.com
Phone
-
Journal Mail Official
imart171@yahoo.com
Editorial Address
-
Location
Kota bogor,
Jawa barat
INDONESIA
Current Biochemistry
Published by Institut Pertanian Bogor
ISSN : 23557877     EISSN : 23557931     DOI : -
Core Subject : Science, Education,
Biochemistry, Genetics & Molecular Biology Education
Current Biochemistry (CB) publishes the results of original research that contribute significantly to the understanding of the chemical compound and reaction that occur within living organism. Preference will be accorded to manuscripts that develop new concepts or experimantal approaches, particularly in the advancing areas of biochemistry science. Manuscripts that are primarily theoretical in nature or in the field of bioinformatics must be directed toward explaining important results previously not understood, making important predictions that can be experimentally tested, or developing segnificant advances in theory of general interest to biochemists. Submission of manuscripts in emerging areas in biochemistry, chemical biology, biophysics, proteomics, model studies and structures, cellular and molecular biology, computational biochemistry, biotechnology, and new methods development is encouraged especially if they address basic biochemical mechanisms.
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol. 6 No. 2 (2019)" : 5 Documents clear
Potency of Ethanol Extracts of Palm Seeds (Phoenix dactylifera L.) as Antidiabetic with Inhibition Kinetics Parameter Lisa Giovanny; Faliha Arinda Lestari; Nurul Marfira; Laksmi Ambarsari; Siti Warnasih
Current Biochemistry Vol. 6 No. 2 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.2.1

Abstract

Dates seeds are one of the byproducts of the date palm juice industry which has the potential for further processing. Dates seeds contain active compounds that are known to have ability to inhibit αglucosidase. This study aims to measure the activity of 70% ethanol extract and the fraction of date palm seeds with a mechanism in inhibiting αglucosidase. The measurement of inhibitory activity was carried out by extracting date palm seeds using 70% water and ethanol. The extraction product is then fractionated with n-hexane, ethyl acetate, n-butanol, and water. Then the total phenolic and flavonoid calculations were calculated for each extract and fraction. Measurement of α-glucosidase inhibition activity was measured based on reaction of substrate to enzyme by the addition of inhibitors. The results showed that the ethanolextract had the highest total phenolic and flavonoid, which were 76.86 mg GAE / g and 21.19 mg QE / g, respectively. n-hexane fraction have the best inhibitory activity seen from the IC50 value, which is 12.69 mg/L. The kinetics of inhibition of 70% ethanol extract of date palm seeds are mixed inhibition. Keywords: α-glucosidase, date seeds, flavonoids, kinetic inhibition, phenolics
Potency Evaluation of Nanoparticles Moringa Leaves Extract as a Bioactive Candidate of Eco-Friendly Antifouling Paint Laksmi Ambarsari; Riksa Nur Wahyuni; Agung Isnanto; Rifany Fairuz Aqilah
Current Biochemistry Vol. 6 No. 2 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.2.2

Abstract

Biofouling has become a serious problem for the maritime industry. To minimize the impact of biofouling are protected by antifouling coating made from Tributytlin - polishing copolymer paints (TBT - SPC paint) which are heavy metal groups. New alternative to replace the antifouling material is with the flavonoids in Moringa leaf extract. The objective of this research to analyze the potential of Moringa leaf ethanol extract, effectiveness and stability of Moringa leaves ethanol extract as antifouling and its potential to be a bioactive candidate in antifouling coating. Moringa leaves were extracted by maceration method using 96% ethanol and the yield was obtained about 9.32%. Flavonoid compounds were isolated using a liquid-liquid extraction method and obtained a yield of 60.11%. Flavonoid compounds are encapsulated in nanoparticles with chitosan. PSA analysis shows particle size nanoparticle moringa leaf extract of 31.26 nm. Activity of antifouling is determined by inhibition and degradation test using P. aeruginosa and B. subtilis. Moringa leaf nanoparticles and Moringa leaf flavonoid extract caused biofilm inhibition of 43.55% and 10.29% for P. aeruginosa, while for B. subtillis 25.85% and 82.58%. The result of biofilm degradation by Moringa leaf nanoparticles and Moringa leaf flavonoid extract was 6.9% and 2.2% for P. aeruginosa, while B. subtillis was 87.85% and 65.91%. Keyword: antifouling coat, moringa leaves, nanoparticles.
Antibacterial Activity of Lemongrass (Cymbopogon nardus) Ethanolic Extract Waras Nurcholis; Mira Takene; Ratna Puspita; Lisnawati Tumanggor; Eka Nurul Qomaliyah; Muhammad Maftuchin Sholeh
Current Biochemistry Vol. 6 No. 2 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.2.4

Abstract

Infectious diseases are a disease caused by bacteria such as Escherichia coli and Staphylococcus aureus. One way to overcome the disease by using antibiotics. However, wise use can lead to antibiotic resistance, so many bacteria are resistant to antibiotics. Therefore it is necessary to search the natural compounds as antibacterials, one of them by using lemongrass (Cymbopogon nardus). This study aims to determine the effectiveness of leaf extracts, stems, and roots lemongrass as antibacterial. The method used in this research is the disc method. Bacteria used are E. coli and S. aureus. The results show extracts of roots, stems, and leaf has activity against S. aureus, while E. coli shows no activity as antibacterial, it can be possible because of the different properties of the bacteria. Keywords: anti-bacteria, lemongrass, Escherichia coli, Staphylococcus aureus
Toxicity Test of Roots, Stems and Leaves of Lemongrass (Cymbopogon nardus) Waras Nurcholis; Mustika Weni; Rizki Fitria; Najmah; Kornelia Rosvita Manek; Baharuddin Yusuf Habibie
Current Biochemistry Vol. 6 No. 2 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.2.6

Abstract

Lemongrass traditionally can be used as a mixture of water bath for rheumatic patients, antiseptic medicine, headache reducer, unbend the leech bite and overcome insect bite, but, the bioactivity potential of citronella lemongrass still remains unknown. The content of citronella lemongrass is known to have secondary metabolites such as flavanoid, phenolic, and terpenoid. The aim of this study is to determine the LC50 value (Lethality Concentration 50) in the root, stem, and leaves part of citronella lemongrass. Citronella lemongrass is extracted with maceration method using 96% ethanol solvent. Rendement value of leaf extract is greater than the value of stem and root extract, which is equal to 8.69% for stem extract, 3.73% for root extract and 9.70% for leaves extract. A toxicity test was performed by using the BSLT method, shrimp larvae were inserted into the test solution with each different solution concentration. LC50 values were obtained based on the calculation of percent mortality of shrimp larvae by using probit analysis. LC50 ethanol extract 96% of citronella lemongrass leaf is 67.841 μg/ml, citronella lemongrass stem is 31.604 μg/ml and citronella lemongrass root is 10.489 μg/ml. LC50 value shows that ethanol extract 96% of root, stem, and leaf of citronella lemongrass have no bioactivity potential due to the value of LC50 is greater than 1000 μg/ml. Keywords: BSLT, Cymbopogon nardus, cytotoxicity, LC50
N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro Lisni Noraida; Maria Bintang; Bambang Pontjo Priosoeryanto
Current Biochemistry Vol. 6 No. 2 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.2.5

Abstract

Green tea (Camellia sinensis) is one of tradisional plants that have potential as anticancer. Thesample used in this research was commercial green tea extract. The purpose of this study was to test theantiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract wasfractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and water fraction of greentea have Lethality Concentration 50 (LC50) value of more than 1000 ppm. Fractions of ethanol 70% and nhexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of phytochemicalscreening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screeningresults of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancercells MCM-B2 and normal cells Vero by tripan blue staining method. The highest MCM-B2 cell inhibitoryactivity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction,59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Verocells characterized by not inhibiting normal cell proliferation.

Page 1 of 1 | Total Record : 5

 1 

Filter by Year

2019 2019


Reset
Filter By Issues
All Issue Vol. 12 No. 1 (2025): Vol. 12 No. 1 (2025) Vol. 11 No. 1 (2024) Vol. 10 No. 2 (2023) Vol. 10 No. 1 (2023) Vol. 9 No. 2 (2022) Vol. 9 No. 1 (2022) Vol. 8 No. 2 (2021) Vol. 8 No. 1 (2021) Vol. 7 No. 2 (2020) Vol. 7 No. 1 (2020) Vol. 6 No. 2 (2019) Vol. 6 No. 1 (2019) Vol. 5 No. 3 (2018) Vol. 5 No. 2 (2018) Vol. 4 No. 3 (2017) Vol. 4 No. 2 (2017) Vol. 4 No. 1 (2017) Vol. 3 No. 3 (2016) Vol. 3 No. 2 (2016) Vol. 3 No. 1 (2016) Vol. 2 No. 3 (2015) Vol. 2 No. 2 (2015) Vol. 2 No. 1 (2015) Vol. 1 No. 3 (2014) Vol. 1 No. 2 (2014) Vol. 1 No. 1 (2014) More Issue