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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

126 127 128 129 130

Molecular analysis of hemaglutinin gene of Avian Influenza viruses isolated in 2012-2013 Sussi Widi Kurniasih; Retno D. Soejoedono; N.L.P.I. Mayasari
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 2 (2015): JUNE 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Avian Influenza virus (AIV) still plays as a major cause of the death in poultry in Indonesia and around the world. The aim of this research was to determine the pathogenicity and to analyze the phylogenetic and genetic distances of hemagglutinin gene of isolated AI viruses in Indonesia in 2012-2013 particularly from West Java, Central Java, and North Sumatra. Samples were obtained from poultry farms that suffered from AI outbreaks, were inoculated and propagated in ten days old specific pathogen free (SPF) embryonated chicken eggs. Harvested allantoic fluids at 5 days after inoculation were tested for hemagglutination activity. Positive allantoic fluids were further tested to determine the hemagglutinin and neuraminidase subtype using real-time reverse transcription polymerase chain reaction (RRT-PCR) and to be prepared for sequencing using reverse transcription polymerase chain reaction (RT-PCR). The sequence of hemagglutinin genes were analyzed for the amino acid pattern of the cleavage site region and the genetic distances and relationships of those viruses. The result indicated that all of the isolates are classified as HPAI with the pattern of cleavage site regions are QRESRRKKR and QRERRRKR. Six isolates are classified as H5N1 and 3 isolates are H5Nx. All of the isolates have close genetic relationship with the genetic distances less than 0.3 between one to another and also with several AI viruses that caused previous outbreaks in Indonesia.

Development of alpha hemolytic verotoxigenic Escherichia coli vaccine: Verotoxic antibody responses in experimental animals, mice, rabbits and dairy cows Supar .; B Peorwadikarta; Nina Kurniasih; Djaenuri .
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 1 (1999): MARCH 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Escherichia coli isolates showing alpha hemolytic on blood agar were found in calves with bloody diarrhoea from Bandung, Sukabumi and Bogor. Three E. coli isolates designated as (B34c, B909, B910) were pathogenic for mice and selected for vaccine candidates. Crude supernatant of tryptic soy broth (TSB) cultures precipitated with saturated ammoniumsulphate caused cytopathic effect on vero cell line. For vaccine preparation the isolates were grown on tryptic soy agar (TSA). Whole cell inactive vaccine containing equal proportion of each isolate was prepared and emulsified in aluminum hydroxide gel at a final concentration of 1.5% and cell concentration equal to the tube number 10 of MacFarland standard. Mice and rabbits were injected subcutaneously of monovalent vaccine with the dose of 0.2 ml and 1 ml respectively. Four weeks later mice or rabbits were boostered with the same dose. Before injection each animal was bleed, subsequently every two weeks period up to 4 weeks after the second injection. The sera were separated and kept at -20oC up to serological assay with an enzyme linked immunoassay (ELISA) was done. Vaccinated mice with VTEC were challenged with homologous isolate did not die, where as 60% of unvaccinated mice died within 2 days post challenged. Pregnant cows were given 2 doses of three valent vaccine 6 weeks and again 2 weeks before expected date of calving. Calves born were given colostrum and milk of the own mother. The three day old calf was challenged with three isolates of verotoxigenic E. coli studied. Calves born from vaccinated cows did not show diarrhoea at post challenged, whereas calf from unvaccinated cow demonstrated bloody diarrhoea at post challenged. From the experimental animals (mice and calves) demonstrated the presence of protection against challenged. The antiverotoxic antibody probably involved in important role of immunoprotection. These were supported by the presence of high titer anti verotoxic antibody in serum of experimental animals (mice and cows), as well as in experimental rabbits, but none in the unvaccinated animals. Key words: E. coli, alpha hemolytic, verotoxic, dysentery, vaccine

A study of inoculation route and dosage levels on embryonated chicken eggs as media for testing tea mistlestoe (Scurrula oortiana) extract activity Sri Murtini; R Murwarni; F Satrija; M.B.M Malole
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Tea mistlestoe extract (Scurrula oortiana) has cytotoxic activity which is potential to be used in preventing viral induced-chicken tumor. The following study was designed to evaluate the effects of different inoculation routes, dosage levels, and strains of embryonated chicken eggs as media for testing the tea mistlestoe extract (Scurrula oortiana) antiviral activity. Proper inoculation route was examined by inoculation of the extract at dose level of 0,2 mg/egg into embryonated layer eggs via allantoic cavity, chorio-allantoic membrane, and yolk sac. Effect of dose level of tea mistlestoe extract on embryo development was examined in groups of embryonated broiler eggs inoculated with the extract at 0.02, 0.2, 2, 20, or 200 mg/egg. Inoculation of tea mistlestoe extract into allantoic cavity was the safest procedure as indicated by the absence of embryos mortality, and faster embryo growth compared to those of chorio-allantoic membrane and yolk sac-inoculated eggs. The extract induced different growth effects when inoculated into embryonated layer or broiler eggs. Administration of the extract at dose levels between 0,02–200 mg/egg reduced significantly the weight of broiler embryoes, but not the relative weights of liver, heart and spleen. Administration of similar dosage in layer embryoes did not cause any significant difference in the embryoes weight. This study suggests that the study of antiviral activity of tea mistlestoe extract in embryonated chicken eggs should be carried out on embryonated eggs of layer breeds and the extract should be inoculated via allantoic cavity. Key Words: Scurrula oortiana, Embryonated Chicken Eggs, Enti Viral Activity

Effect of restricted feeding on productivity of Mojosari x Alabio cross-bred layer ducks (MA): 2. Second phase of laying from 44-67 weeks old P.P Ketaren; L.H Prasetyo
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 2 (2002): JUNE 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Productivity and feed efficiency of Mojosari Alabio (MA) cross-bred duck on the first phase of laying from 20-43 weeks old was better when fed ad lib. using pelleted diet. An experiment was conducted to evaluate effect of restricted feeding of pelleted diet on second phase of laying of MA cross-bred duck performances from 44-67 weeks old. The experiment was designed in a Completely Randomized Design. 156 MA cross-bred ducks were devided into three groups and fed three experimental diets: (1) 70% ad lib., (2) 85% ad lib. and (3) ad lib. All experimental diets were fed to ducks for 24 weeks and feed intake, egg production, egg weight, FCR, molting, egg yolk weight, albumen weight, egg shell weight and egg yolk colour score were measured as parameters. The results showed that the restricted feeding of 70 and 85% significantly (P<0.05) reduced egg production, egg weight and egg yolk percentage, egg yolk colour score and induced early molting, but did not adversely affect egg white and egg shell percentage. FCR of duck fed 70% (6.47) was significantly (P<0.05) the worst compared to the duck fed 85% (4.13) and ad lib. (3.55). The results indicate that MA cross-bred duck was more efficient being fed ad lib. Key words: Restricted feeding, MA crossbred duck, productivity and egg quality

Utilization of FecJ F gene in developing commercial sheep farming : Economic analysis Atien Priyanti; I Inonou; B Tiesnamurti
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 1 (1996)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An increase of income per capita in Indonesia is not followed by an increase of numbers and quality of lambs stock. To meet he high demand both for domestic and international markets, sheep production should be elaborated commercially . The Research Institute for Animal Production, Bogor has been able to identify the segregation of FecJ F on Javanese sheep, which has large effect on ovulation rate and number of litters born. The study was purposed to analyse the economic value of using Fed gene and the crossing with St. Croix rams to obtain high number of lamb production as well as high pre-weaning growth rate . Sixty seven Garut ewes were used and classified into three classes of singles, twins and triplets or mom: lambs born . From each litter type, ewes were classified according to the breed of rams to be used . Gamt and St . Croix rams were used to represent small and large size of sires, respectively . The parameters observed were litter size, birth weight, sex, feed consumption, weaning weight and average daily gain . Lambs and ewes were weighed on biweekly and monthly basis, respectively. An increasing of input for single born ewes was not followed by dramatic increase in its body weight at weaning, which means that the optimum level of production was not achieved. This resulted a shortening of farmers income. However, for ewes having twins an increase of input was followed by significantly increased of production level . Therefore, the economic analysis model used for ewes that carry the Fed gene showed an increase of income of Rp.30,691 .50, and Rp.24,319.82, per ewe per period for St. Croix sires and Garut sires, respectively . Key words: Economic analysis, Fed gene, sheep

The effect of fermentation of sludge with Neorospora sp on the egg performances and quality of laying hens Yosi Fenita; Urip Santoso; Hardi Prakoso
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 2 (2010): JUNE 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The purpose of this research is to produce enriched eggs (high concentration in protein, amino acid and β-karoten) but law in lipid and cholesterol content. An experiment was conducted to study the utilization of fermented palm oil sludge (LSF) using neurospora sp for layer. The present study was done based on Randomized Design in which 100 layer were distributed to five treatment group as follow: Layers were fed diet with (0, 5, 10, 15 and 20% LSF). Experimental results showed that LSF significantly (P < 0.05) affected egg mass production, feed comsumption, feed conversion, and HU and highly affected (P < 0.01) yolk colour. The best treatment was with 10% LSF. LSF had no effect on egg weight, shell thickness, albumen index and yolk index (P > 0.05). LSF reduced egg smell, improved taste of egg and yolk colur (P < 0.05). Protein and fat, contents of eggs were significantly reduced (P < 0.05) and cholesterol content of eggs was significantly reduced (P < 0.01). Utilization LSF increased the content of β-karoten yolk (P < 0.01), and modified the composition of amino acid in yolk. In conclusion, utilization of LSF improved egg quality, reduced egg cholesterol dan lipid and modified the composition of amino acid in yolk. Key Words: Palm Oil Sludge, Neorospora sp, Egg Quality

Seroepidemiology of Canine parvovirus infection in dogs Indrawati Sendow; Tatty Syafriati
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 3 (2004): SEPTEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Canine parvovirus is an acute and fatal viral disease in dogs. A total of 209 local, cross breed and breed dogs sera from Kodya Bogor, Kabupaten Bogor, Sukabumi, and Jakarta, had been tested using Haemagglutination Inhibition Test (HI) with pig red blood cells. A total of 64 breed and cross breed dogs from Sukabumi and Kodya Bogor, were used as a sentinel dogs to study the epidemiology of Canine parvovirus (CPV) infection and its immunological responses caused by vaccination. The results indicated that 78% (95) breed and cross bred dogs and 59% (51) local dogs had antibody to CPV. Sentinel dogs results indicated that dogs had been vaccinated showed antibody response with the varied titre dependant upon prevaccination titre. Low prevaccinated titre gave better response than protective level titre. From 19 puppies observed, Maternal antibodi were still detected until 5 weeks old puppies. First vaccination given at less than 3 months old, should be boosted after 3 months old puppied. Antibodi titre produced by natural infection will keep untill 2 years. These data concluded that the dog condition and time of vaccination will affect the optimum antibody response. Key words: Canine parvovirus, haemagglutination inhibition, isolation

Studies on the transmission of malignant catarrhal fever in experimental animals: A serial infection of cattle and buffalo by means of whole blood inoculation Agus Wiyono; Rini Damayanti
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 4 (1999): DECEMBER 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Malignant catarrhal fever (MCF) is a fatal disease especially affecting cattle and buffaloes. A study on the serial blood transmission of MCF was conducted by injecting whole blood of MCF animals into 9 experimental animals. Diagnosis of MCF was based on the clinico-pathological fmdings and polymerase chain reaction (PCR) test. The disease has successfully, been achieved in six animals of three Bali cattle and three buffaloes but not in a Bali-cross breed and two Bos indicus (Ongole) cattle. Wide range of clinical signs and gross-pathological features were observed. The study showed the degree of susceptibility of experimental animals: Bali cattle and buffalo were highly susceptible (3 out of 3 affected with MCF), Bali-cross breed and Bos indicus (Ongole) cattle seemed not susceptible to whole blood experimental transmission. It shows that when Bali cattle acted as inoculum donor, buffalo tended to be clinically more severe than Bali cattle. On the other hand, when buffalo acted as inoculum donor, Bali cattle suffered from MCF more severe than buffalo. The diagnosis of MCF by histopathological examination and the PCR test bad positive correlation (100%) in the first experiment, while in the second experiment the PCR test tends to be more sensitive. Based on the restriction endonuclease (RE) test, the MCF causal agent in this study appeared to be genetically similar in each case. It is concluded that the serial experimental transmission of MCF by means of whole blood inoculation has been successfully achieved in Bali cattle and buffalo but not in Bali-cross breed and Ongole cattle, and there is a positive correlation between the PCR test and histopathological examination with the PCR test tends to be more sensitive. Key words: MCF, serial transmission, blood, cattle, buffalo, Indonesia

Variation on DNA microsatellite of Holstein-Friesian dairy cows in Baturaden Dairy Cattle Breeding Center C Sumantri; A Anggraeni; A Farajallah; D Perwitasari
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Eight microsatellite loci of which the locations were three on chromosome 6 (BTA6) namely CSN 3, BM 143 and BM 415; two on chromosome 9 (BTA9) namely ETH 225 and BM 4208; and three on chromosome 10 (BTA10) namely BP 31, BM 1237 and BM 888 were used to investigate genotypic variation of Holstein-Friesian (HF) dairy cows in Baturraden Dairy Cattle Breeding Centre. Research activities were carried out through some steps involving blood collection, DNA isolation, amplification on DNA fragments by polymerase chain reaction (PCR) and separation by electrophoresis with silver staining. Frequency and heterozygosity of genes under consideration were calculated. The results showed that the eight microsatellite loci exhibited a total number of 33 alleles. Locations of those alleles were five in BM 143 and BM 4208 loci; four in BM 415, CSN 3, ETH 225, BM 1237, BM 888 loci; and three in BP 31 locus. A number of 14 out of 33 alleles might be specific alleles for HF dairy cattle in Baturraden Dairy Cattle Breeding Centre. The lowest heterozygosity per locus (ĥ) was 0.6151 for BM 415 whilst the highest one was 0.7301 for BM 888. Additionally, the average heterozygosity for all loci (Ĥ) detected in this study was 0.6768. The genotype BB on locus BM 143 and AC on locus CSN-3 significantly affected (P<0.05) the estimated breeding value of milk yield of HF cows in this location. Key Words: Holstein-Friesian Cows, DNA Microsatellite, Allele, EBV of Milk Yield

Exploration and conservation of bacterial genetic resources as bacteriocin producing inhibitory microorganisms to pathogen bacteria in livestock Chotiah S
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 2 (2013): JUNE 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Exploration and conservation of microorganisms producing bacteriocin was done as the primary study towards the collection of potential bacteria and its application in improving livestock health condition and inhibit food borne pathogens. Diferent kinds of samples such as beef cattle rectal swab, rumen fluids, cow’s milk, chicken gut content, goat’s milk were collected at Bogor cattle slaughter houses, poultry slaughter houses, dairy cattle and goat farms. A total of 452 bacterial isolates consisted of 73 Gram negative bacteria and 379 Gram positive bacteria were isolated from samples collected and screened for bacteriocin activity. Determination of bacteriocin activity with bioassay using agar spot tests were carried out on liquid and semisolid medium assessing 8 kins of indicators of pathogenic bacteria and food borne pathogens. A total of 51 bacteriocin producing strains were collected and some of the strains had high inhibitory zone such as Lactobacillus casei SS14C (26 mm), Enterobacter cloacae SRUT (24mm), Enterococcus faecalis SK39 (21mm) and Bifidobacterium dentium SS14T (20mm) respectively, to Salmonella typhimurium BCC B0046/ATCC 13311, E. coli O157 hemolytic BCC B2717, Listeria monocytogenes BCC B2767/ATCC 7764 and Escherichia coli VTEC O157 BCC B2687. Evaluation after conservation ex situ to all bacterocin producing strain at 5oC for 1 year in freeze drying ampoules in vacuum and dry condition revealed the decreasing viability starting from log 0.8 CFU/ml for Lactococcus and Leuconostoc to log 2.2. CFU/ml for Streptococcus. Result of the study showed that the bacteriocin producing strains obtained were offered a potential resource for preventing disease of livestock and food borne diseases. Key Words: Bacterocin, Bacteria, Exploration, Conservation Ex Situ

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Ahmadi Riyanto

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ahmadi_puslitbangnak@yahoo.com

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Abstract

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Journal Mail Official ahmadi_puslitbangnak@yahoo.com

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Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA