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Prevalence of antibody against infectious bovine rhinotracheitis virus in sentinel cattle in West and East Nusa Tenggara Wiyono, Agus; Sarosa, Antonius; Goeng, Maria
Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 2 (1995)
Publisher : Indonesian Animal Sciences Society

A total of 4,977 sera from a sentinel cattle in West and East Nusa Tenggara were tested for antibody against BHV-1, the causal agent of infectious bovine rhinotracheitis (IBR). These sera were collected between June 1990 and June 1993, and were tested by using serum neutralization test (SNT). Out of these sera, 3,713 were suitable for IBR SNT. A total of 349 sera (10.4%) reacted. IBR reactors were more prevalent in East Nusa Tenggara (NTT) than in West Nusa Tenggara (NTB) . Based on this survey, it is concluded that antibodies against IBR virus are present among cattle in East and West Nusa Tenggara . Keywords : SNT, BHV-1, IBR, sentinel cattle

Isolation and characterization of virus of highly pathogenic avian influenza H5 subtype of chicken from outbreaks in Indonesia Wiyono, Agus; Indriani, R; Dhamaryanti, N.L.P.I; Damayanti, R; Parede, L; Syafriati, T; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 1 (2004)
Publisher : Indonesian Animal Sciences Society

A study on the isolation and characterization of Highly Pathogenic Avian Influenza of chicken from outbreaks in Indonesia was conducted at Indonesian Research Institute for Veterinary Science. Outbreaks of avian disease had been reported in Indonesia since August 2003 affecting commercial layer, broiler, quail, and ostrich and also native chicken with showing clinical signs such as cyanosis of wattle and comb, nasal discharges and hypersalivation, subcutaneous ptechiae on foot and leg, diarre and sudden high mortality. The aim of this study is to isolate and characterize the causal agent of the disease. Samples of serum, feather follicle, tracheal swab, as well as organs of proventriculus, intestine, caecal tonsil, trachea and lungs were collected from infected animals. Serum samples were tested haemaglutination/haemaglutination inhibition to Newcastle Disease and Egg Drop Syndrome viruses. Isolation of virus of the causal agent of the outbreak was conducted from samples of feather follicle, tracheal swab, and organs using 11 days old specific pathogen free (SPF) embryonated eggs. The isolated viruses were then characterised by agar gel precipitation test using swine influenza reference antisera, by haemaglutination inhibition using H1 to H15 reference antisera, and by electron microscope examination. The pathogenicity of the viruses was confirmed by intravenous pathogenicity index test and its culture in Chicken Embryo Fibroblast primary cell culture without addition of trypsin. The study revealed that the causative agent of the outbreaks of avian disease in Indonesia was avian influenza H5 subtype virus based upon serological tests, virus isolation and characterization using swine influenza reference antisera, and electron microscope examination. While subtyping of the viruses using H1 to H15 reference antisera suggested that the virus is very likely to be an avian influenza H5N1 subtype virus. The pathogenicity test confirmed that the viruses are highly pathogenic to experimental animals. It is concluded that the causative agent of the outbreaks of avian disease in Indonesia was avian influenza H5 subtype virus. The result has been the basis of further study such as development serological tests and vaccine production. The decission of Indonesian Government to conduct vaccination program using homolog vaccine in order to control the disease is regarded as the correct choice. However, it should be accompanied by conducting surveillance and monitoring of the disease as well as the possibility of mutation of virus. The program should be coordinated nationally. Â Key words: Virus isolation, characterization, chicken, outbreak, highly pathogenic avian influenza (HPAI), H5 subtype, Indonesia

The detection of ovine herpesvirus-2 in reservoir host of malignant catarrhal fever in Indonesia by means of polymerase chain reaction Wiyono, Agus
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 2 (1999)
Publisher : Indonesian Animal Sciences Society

Malignant catarrhal fever (MCF) is a fatal disease affecting large ruminants, some wild animals and pigs. Ovine herpesvirus- 2 (OHV-2) is the causal agent of sheep-associated MCF (SA-MCF). In Indonesia, sheep is epidemiologically implicated in the occurrence of MCF. Providing a sensitive and specific molecular biological technique, i.e. polymerase chain reaction (PCR), a wide range of MCF aspects on molecular level have been developed. The PCR has been successfully used to detect OHV-2 fragment in sheep samples such as peripheral blood leucocytes (PBL) of sheep, nasal secretion of a sheep lamb, and organs of some sheep lambs. Fragment of OHV-2 was also detected in PBL of goats from some areas in Indonesia but not in PBL of pigs from Bali. These results suggest that sheep and sheep lambs are the potential reservoir hosts of MCF in Indonesia, while the role of goats as reservoir host of MCF has yet to be determined. Â Key words : Ovine herpesvirus-2, polymerase chain reaction, reservoir host, malignant catarrhal fever, Indonesia

Studies on the transmission of malignant catarrhal fever in experimental animals: Bali cattle in close contact with sheep Wiyono, Agus; Damayanti, Rini
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 2 (1999)
Publisher : Indonesian Animal Sciences Society

pregnant sheep and lambing sheep were conducted in two different periods and flocks of Bali cattle and sheep. On the basis of clinico-pathological findings and polymerase chain reaction (PCR) tests, the study revealed that MCF transmission has been successfully achieved in four Bali cattle that were in contact with lambing sheep, and in one Bali cow that was penned 100 metres away from lambing sheep pen. The polymerase chain reaction (PCR) detected the causal agent of MCF in peripheral blood leucocytes (PBL) and nasal, ocular and vaginal secretions of sheep. It is assumed that viruses in the secretion were spread by sheep causing five Bali cattle suffered with MCF. However, further studies on exact mode of transmission needs to be clarified particularly on the time and mode of viral transmission from reservoir to susceptible animals. Â Key words : Malignant catarrhal fever, experimental transmission, in-contact, Bali cattle, sheep

The detection of ovine herpesvirus-2 in reservoir host of malignant catarrhal fever in Indonesia by means of polymerase chain reaction Wiyono, Agus
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 2 (1999)
Publisher : Indonesian Animal Sciences Society

Malignant catarrhal fever (MCF) is a fatal disease affecting large ruminants, some wild animals and pigs. Ovine herpesvirus- 2 (OHV-2) is the causal agent of sheep-associated MCF (SA-MCF). In Indonesia, sheep is epidemiologically implicated in the occurrence of MCF. Providing a sensitive and specific molecular biological technique, i.e. polymerase chain reaction (PCR), a wide range of MCF aspects on molecular level have been developed. The PCR has been successfully used to detect OHV-2 fragment in sheep samples such as peripheral blood leucocytes (PBL) of sheep, nasal secretion of a sheep lamb, and organs of some sheep lambs. Fragment of OHV-2 was also detected in PBL of goats from some areas in Indonesia but not in PBL of pigs from Bali. These results suggest that sheep and sheep lambs are the potential reservoir hosts of MCF in Indonesia, while the role of goats as reservoir host of MCF has yet to be determined. Â Key words : Ovine herpesvirus-2, polymerase chain reaction, reservoir host, malignant catarrhal fever, Indonesia

Studies on the transmission of malignant catarrhal fever in experimental animals: A serial infection of cattle and buffalo by means of whole blood inoculation Wiyono, Agus; Damayanti, Rini
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 4 (1999)
Publisher : Indonesian Animal Sciences Society

Malignant catarrhal fever (MCF) is a fatal disease especially affecting cattle and buffaloes. A study on the serial blood transmission of MCF was conducted by injecting whole blood of MCF animals into 9 experimental animals. Diagnosis of MCF was based on the clinico-pathological fmdings and polymerase chain reaction (PCR) test. The disease has successfully, been achieved in six animals of three Bali cattle and three buffaloes but not in a Bali-cross breed and two Bos indicus (Ongole) cattle. Wide range of clinical signs and gross-pathological features were observed. The study showed the degree of susceptibility of experimental animals: Bali cattle and buffalo were highly susceptible (3 out of 3 affected with MCF), Bali-cross breed and Bos indicus (Ongole) cattle seemed not susceptible to whole blood experimental transmission. It shows that when Bali cattle acted as inoculum donor, buffalo tended to be clinically more severe than Bali cattle. On the other hand, when buffalo acted as inoculum donor, Bali cattle suffered from MCF more severe than buffalo. The diagnosis of MCF by histopathological examination and the PCR test bad positive correlation (100%) in the first experiment, while in the second experiment the PCR test tends to be more sensitive. Based on the restriction endonuclease (RE) test, the MCF causal agent in this study appeared to be genetically similar in each case. It is concluded that the serial experimental transmission of MCF by means of whole blood inoculation has been successfully achieved in Bali cattle and buffalo but not in Bali-cross breed and Ongole cattle, and there is a positive correlation between the PCR test and histopathological examination with the PCR test tends to be more sensitive. Â Key words: MCF, serial transmission, blood, cattle, buffalo, Indonesia

EVALUASI KUALITAS PELAYANAN HALTE DAN PENGEMBANGANNYA DI KOTA SURABAYA UNTUK MENDUKUNG TERWUJUDNYA INFRASTRUKTUR BERWAWASAN LINGKUNGAN Widayanti, Ari; Susanti, Anita; Wiyono, Agus
Prosiding Seminar Nasional Sains dan Teknologi Terapan Inovasi Teknologi Infrastruktur Berwawasan Lingkungan
Publisher : Institut Teknologi Adhi Tama Surabaya

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Pembangunan dan pengembangan infrastruktur untuk mendukung aktivitas masyarakat sedang mendapatkan perhatian dari Pemerintah saat ini. Halte merupakan salah satu infrastruktur perkotaan yang sangat diperlukan bagi pengguna angkutan umum. Kondisi halte perlu dievaluasi dan dikembangkan agar dapat berfungsi secara optimal dan sesuai dengan kebutuhan masyarakat menuju terwujudnya infrastruktur yang berwawasan lingkungan.Tujuan studi ini adalah mengetahui kualitas pelayanan halte sehingga dapat dikembangkan sesuai dengan konsep infrastruktur yang berwawasan lingkungan. Metode pengumpulan data dengan observasi lapangan dan wawancara serta membagikan Â kuisioner kepada 130 orang pengguna. Teknik analisis data berupa deskriptif kuantitatif dan Importance Performance Analysis (IPA).Hasil yang diperoleh adalah kualitas pelayanan halte masih belum memuaskan dengan nilai x = 3,62 dan y = 4,63. Atributâ€“ atribut yang berada dalam kuadran A harus lebih diprioritaskan dan diperbaiki, sehingga kinerjanya meningkat dan menjadi lebih baik lagi, karena atribut â€“ atribut ini memiliki nilai kepentingan yang tinggi bagi konsumen, namun kinerjanya masih kurang memuaskan. Atributâ€“atribut yang berada pada kuadran ini yaitu memberikan keamanan dan kenyamanan bagi pengguna halte, kepedulian pengguna halte terhadap kebersihan, kemudahan untuk menjangkau halte dan angkutan umum. Dalam konsep pengembangan infrastruktur yang berwawasan lingkungan diperlukan disain bangunan halte yang berkonsep green infrastructure, interkonektivitas yang lebih baik dengan angkutan umum dan jalur pejalan kaki, ketersediaan tempat sampah yang mencukupi, jalur hijau yang semakin baik dan indah, dan tempat duduk yang nyaman dan aman.

Studies on the transmission of malignant catarrhal fever in experimental animals: Bali cattle in close contact with sheep Agus Wiyono; Rini Damayanti
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 2 (1999): JUNE 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

pregnant sheep and lambing sheep were conducted in two different periods and flocks of Bali cattle and sheep. On the basis of clinico-pathological findings and polymerase chain reaction (PCR) tests, the study revealed that MCF transmission has been successfully achieved in four Bali cattle that were in contact with lambing sheep, and in one Bali cow that was penned 100 metres away from lambing sheep pen. The polymerase chain reaction (PCR) detected the causal agent of MCF in peripheral blood leucocytes (PBL) and nasal, ocular and vaginal secretions of sheep. It is assumed that viruses in the secretion were spread by sheep causing five Bali cattle suffered with MCF. However, further studies on exact mode of transmission needs to be clarified particularly on the time and mode of viral transmission from reservoir to susceptible animals. Key words : Malignant catarrhal fever, experimental transmission, in-contact, Bali cattle, sheep

Isolation and characterization of virus of highly pathogenic avian influenza H5 subtype of chicken from outbreaks in Indonesia Agus Wiyono; R Indriani; N.L.P.I Dhamaryanti; R Damayanti; L Parede; T Syafriati; Darminto .
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 1 (2004): MARCH 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study on the isolation and characterization of Highly Pathogenic Avian Influenza of chicken from outbreaks in Indonesia was conducted at Indonesian Research Institute for Veterinary Science. Outbreaks of avian disease had been reported in Indonesia since August 2003 affecting commercial layer, broiler, quail, and ostrich and also native chicken with showing clinical signs such as cyanosis of wattle and comb, nasal discharges and hypersalivation, subcutaneous ptechiae on foot and leg, diarre and sudden high mortality. The aim of this study is to isolate and characterize the causal agent of the disease. Samples of serum, feather follicle, tracheal swab, as well as organs of proventriculus, intestine, caecal tonsil, trachea and lungs were collected from infected animals. Serum samples were tested haemaglutination/haemaglutination inhibition to Newcastle Disease and Egg Drop Syndrome viruses. Isolation of virus of the causal agent of the outbreak was conducted from samples of feather follicle, tracheal swab, and organs using 11 days old specific pathogen free (SPF) embryonated eggs. The isolated viruses were then characterised by agar gel precipitation test using swine influenza reference antisera, by haemaglutination inhibition using H1 to H15 reference antisera, and by electron microscope examination. The pathogenicity of the viruses was confirmed by intravenous pathogenicity index test and its culture in Chicken Embryo Fibroblast primary cell culture without addition of trypsin. The study revealed that the causative agent of the outbreaks of avian disease in Indonesia was avian influenza H5 subtype virus based upon serological tests, virus isolation and characterization using swine influenza reference antisera, and electron microscope examination. While subtyping of the viruses using H1 to H15 reference antisera suggested that the virus is very likely to be an avian influenza H5N1 subtype virus. The pathogenicity test confirmed that the viruses are highly pathogenic to experimental animals. It is concluded that the causative agent of the outbreaks of avian disease in Indonesia was avian influenza H5 subtype virus. The result has been the basis of further study such as development serological tests and vaccine production. The decission of Indonesian Government to conduct vaccination program using homolog vaccine in order to control the disease is regarded as the correct choice. However, it should be accompanied by conducting surveillance and monitoring of the disease as well as the possibility of mutation of virus. The program should be coordinated nationally. Key words: Virus isolation, characterization, chicken, outbreak, highly pathogenic avian influenza (HPAI), H5 subtype, Indonesia

Prevalence of antibody against infectious bovine rhinotracheitis virus in sentinel cattle in West and East Nusa Tenggara Agus Wiyono; Antonius Sarosa; Maria Goeng
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 2 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A total of 4,977 sera from a sentinel cattle in West and East Nusa Tenggara were tested for antibody against BHV-1, the causal agent of infectious bovine rhinotracheitis (IBR). These sera were collected between June 1990 and June 1993, and were tested by using serum neutralization test (SNT). Out of these sera, 3,713 were suitable for IBR SNT. A total of 349 sera (10.4%) reacted. IBR reactors were more prevalent in East Nusa Tenggara (NTT) than in West Nusa Tenggara (NTB) . Based on this survey, it is concluded that antibodies against IBR virus are present among cattle in East and West Nusa Tenggara .

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