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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

96 97 98 99 100

Growth responses of native chicken Sentul G-3 on diet containing high rice-bran supplemented with phytase enzyme and ZnO Cecep Hidayat; Sumiati . .; Sofjan Iskandar
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 3 (2014): SEPTEMBER 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This study was conducted to determine the effect of phytase enzymes and ZnO supplementation on the performance of native chicken Sentul-G3 fed high rice-bran diet. Two hundred and seventy day old chicks (DOC) native chicken Sentul-G3 from three different hatcheries were used in this study. Factorial randomized block design (3 x 3) was applied in this study. The first factor was the enzyme phytase supplementation levels (0; 1000; 2000 U/kg), the second factor was the level of supplementation of ZnO (0; 1.5; 3.2 g/kg), so that there are nine treatment given, namely R1 = 50% commercial diet : 50% rice bran; R2 = R1 + 1.5 g ZnO/kg; R3 = R1 + 3.2 g ZnO/kg; R4 = R1 + phytase enzyme 1000 U/kg; R5 = R1 + (phytase enzyme 1000 U/kg + 1.5 g ZnO/kg); R6 = R1 + (phytase enzyme 1000 U/kg + 3.2 g ZnO/kg); R7 = R1 + phytase enzyme 2000 U/kg; R8 = R1 + (phytase enzyme 2000 U/kg + 1.5 g ZnO/kg); R9 = R1 + (phytase enzyme 2000 U/kg + 3.2 g ZnO/kg). Each experimental unit consisted of 6 head unsexed native chicken Sentul-G3. The experimental diet was fed for 10 weeks. The variables measured were body weight, body weight gain, feed intake, feed conversion ratio, mortality, mineral deposition of Ca, P, Zn in the tibia bone, alkaline phosfatase enzyme activity in serum. Results showed that there was significant interaction (P<0.05) between phytase enzyme and ZnO supplementation on body weight, body weight gain, feed conversion, zinc deposition in the tibia bone. There was no significant interaction (P> 0.05) between phytase enzyme and ZnO supplementation on feed intake, mortality, alkaline phosphatase enzyme activity in serum, and deposition of calcium and phosphorus in the tibia bone. It was concluded that supplementation of phytase enzyme and ZnO were not able to increase the growth of native chicken Sentul-G3 on fed diet containing high rice bran.

Ingestion of host immunoglobulin by Sarcoptes scabiei Simson Tarigan
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Scabies is one of the most important diseases in human and veterinary medicine. The available control measures that rely on acaricides are unsustainable, costly and environmentally unfriendly. Vaccination which is supposedly the most attractive alternative control, is sustainable, potentially cheap and environmentally friendly. Recent development in protein biochemistry and recombinant technology have facilitated the development of anti-parasite vaccine which in the past was impossible. One prerequisite for the anti-parasite-vaccine development is that the parasite has to ingest its host immunoglobulin. This study, therefore, was designed to determine whether Sarcoptes scabiei, a non blood-feeding parasite that resides on the avascular cornified layer of the skin, ingest its host immunoglobulin. Sections of routinely processed mites and skin from a mangy goat were probed with peroxidase-conjugated-anti-goat IgG and the immune complex was visualised with diaminobenzidine solution. To determine whether the ingested IgG was still intact or had been fragmented by the proteolytic enzymes, immunoblotting analysis of SDS-PAGE- fractionated proteins extracted from washed mites was performed. Quantification of IgG was done byan Elisa using purified goat IgG as control. This study showed that IgG in the mites confined to the mite’s gut only, and only a fraction of mite population ingested the IgG. The ingested IgG, as shown by immunoblot analysis, was mostly still intact. This study indicates that development of anti-scabies vaccines is reasonable. Key Words: Sarcoptes scabiei, Immunoglobulin

Effect of age on hepatic lipogenic enzyme activities and fat accumulation in broiler chicks U Santoso; K Tanaka
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 2 (2001): JUNE 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The present study was conducted to evaluate effect of age on hepatic lipogenic enzyme activities and lipid fraction in broiler chicks. Eighty broiler chicks were distributed into one group. Broilers were fed commercial diet and provided drinking water ad libitum. At 5, 10, 12, 15, 17,20,22,25,27, and 56 days of age (27, 42, and 56 days of age for serum and carcass collection), 5 chicks were selected based on weights and killed. Experimental results show that the older chicks would have lower specific activity of hepatic acetyl-CoA carboxylase (ACC) and fatty acid synthetase (F AS) activities (P<0,01). Age influenced hepatic triglyceride (P<0.05), serum cholesterol ester and free cholesterol (P<O.OI). The older chicks had higher serum phospholipid and triglyceride (P<0.01). The older chicks had higher carcass fat and abdominal fat (P<0,01), and lower carcass moisture (P<0.05). In conclusion, the older chicks had lower specific activities of hepatic ACC and FAS (lipogenic enzymes) with higher lipid accumulation. Key words: Age, acetyl-CoA carboxylase, fatty acid synthetase, lipid accumulation, broiler chicks

Leaf area, chlorophyll content, and relative growth rate of grass on different shading and fertilization Juniar Sirait
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Plant morphology and physiology such as leaf area, total chlorophyll, and chlorophyll a/b ratio were affected by shading and fertilization. An experiment was conducted in Indonesian Research Institute for Animal Production (IRIAP) Ciawi, West Java. The objective of this study was to determine the morphology and physiology adaptation of three short grasses species for grazing namely Paspalum notatum, Brachiaria humidicola, Stenotaphrum secundatum on different shading and fertilization level. The experiment was arranged in split-split plot design. The main plot was shading level (0, 38, 56%); sub-plot was fertilizer dosage (0, 100, and 200 kg N/ha) while sub-sub plot was grass species. The results showed that leaf area were significantly different on different grass species, and there was interaction between shading and species on total leaf area. P. notatum on 38% shading level has the highest total leaf area (240.2 cm2). S. secundatum and P. notatum had a better adaptation in shading area compared to B. humidicola by increasing of total chlorophyll and decreasing of chlorophyll a/b ratio. Relative growth rate (RGR) of P. notatum and S. secundatum were increased by the increasing shading level. On the other hand relative growth rate of B. humidicola was decreased by the increasing shading level. Leaf area, chlorophyll content, and RGR were not significantly affected by fertilization dosage. Key Words: Shading, Fertilization, Adaptation, Growth, Chlorophyll

Comparison of four diluents for the retriever dogs semen preservation A. Wicaksono; R.I. Arifiantini
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 1 (2009): MARCH 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The quality of chilled semen depends on the composition of diluent. The choice of the buffer, anti-cold shock and nutrition sources can be the first decision in order to choose appropriate diluents. Nowadays a lot of diluent are used for canine semen preservation such as Tris buffer and Citrate buffer. This study was aimed to observe the differences of diluent for preserving Retriever dog spermatozoa. The semen sample collected from four Retriever dogs with three times repetition. The semen was evaluated macro-and microscopically. The semen with >70% sperm motility was divided into four tubes and diluted with diluter 1 (P1), diluter: P2, P3 and P4 (modified P3). The diluted semen was divided into two tubes and each sample was stored at room and 50C temperature. The viability of chilled semen was observed every 3 hours at room temperature and 12 hours at 50C. The result showed that P2 keep the sperm viability better than the other diluents. On 50C at 24 hours storage P2 showed the highest motile and live sperm percentage (46.25 ± 0.22%; 57.11 ± 0.25%). In room temperature at 6 hours P2 showed the highest motile and live sperm percentage (40.94 ± 0.20%; 52.65 ± 0.23%). It is concluded that P2 can keep the sperm viability by 84 hours of 50C and 21 hours at room temperature. Key Words: Diluents, Dog Sperm, Retriever

Digestion and ruminal fermentation of cocoa pod silage based ration enriched by gliricidia and calliandra leaves on goats Puastuti W; Widiawati Y; Wina E
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 1 (2015): MARCH 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

In term of availability, cacao pod is potential for ruminant feed. According to its nutrients content, cacao pod can be used as feed fiber source. Protein sources materials must be added when cacao pod was ensilaged due to low protein content of this material. The aim of this study was to investigate digestibility value and end products of rumen fermentation of goat fed grass or cacao pod based ration. Randomized block design and 20 heads of lambs (16.95±2.36 kg) to evaluated 5 type of rations: R (50% grass + 50% concentrate); S (50% cacao pod silage + 50% concentrate); SG (50% cacao pod-gliricidia silage + 50% concentrate); SK (50% cacao pod-calliandra silage + 50% concentrate) dan SC (50% cacao pod-mixture of gliricidia-calliandra silage + 50% concentrate). Feeding trial was conducted for over 15 weeks. Measurements were taken on feed digestibility and rumen-fermentation end-products after 3 weeks of treatments. Results shows that nutrients digestibility was different significantly among the groups of treatments (P<0.05). Digestibillity of organic matter, NDF and energy of R ration was those of higher significantly (P<0.05) than those of other groups. N-ammonia of rumen from goat feed R ration was higher (P<0.05) than other groups. Total VFA and each component were different among the groups (P<0.05), however the value was similar among the groups of cacao pod silage rations. It is concluded that cacao pod silaged based rations enriched by Gliricidia and Calliandra leaves did not produce similar digestibility value and end products of rumen fermentation with grass based ration.

Protective antibody titre against Newcastle disease in ostriches (Struthio camelus) Darminto .; Sjamsul Bahri; N. Suryana
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 4 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The aim of this study was to define an estimated antibody titre which was considered to be protective against Newcastle disease (ND) virus infection in ostriches. Eighteen young ostriches of 4 days of age were divided into two groups each containing 9 birds. The first group was unvaccinated and the second group was vaccinated against ND virus twice at 4 and 14 days of age. Antibody titres were monitored at 1, 14, 28, 42, 56, 70 and 85 days of age by haemagglutination inhibition (HI) test. All birds were then challenged with a velogenic strain of ND virus, Ita strain, at 42 days of age. The excretion of the challenge virus were monitored daily after challenge up to the end of this experiment. Several organs such as brain, trachea, lungs and spleen were collected from died birds for re-isolation of the challenged virus. Results indicated that all unvaccinated birds succumbed to the challenged virus, except one bird that survived challenged. In contrast to the unvaccinated birds, all vaccinated birds survived challenged, except two birds with low antibody titres succumbed challenged. All birds with antibody titres of 4 (HI-log2) or greater survived challenged. All challenged birds excreted the challenged virus through out their oropharyngs. Moreover, challenged virus can be successfully re-isolated from most organs of the died birds. This study concludes that : (a) the estimated protective titre against ND in ostriches is 4 (HI-log2), (b) the immune status for ostrich with antibody titre less the 4 (HI-log2) could not be defined, and (c) vaccination against Newcastle disease in ostriches could successfully prevent birds from sick and died of ND, but unable to prevent virus infection and unable to stop carrier status after infection. Key words : Newcastle disease, ostrich, antibody, protective titre

Identification and characterisation of heat-stable allergens from Sarcoptes scabiei Simson Tarigan
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 1 (2006): MARCH 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Animals or human recovered from Sarcoptes scabiei infestation acquired protective immunity against reinfestation. The protective immunity is considered to be associated with a type-1-hypersensitivity reaction against allergens instigated by the mites during infestation. It is assumed that these allergens have the potential to be used as the main component of an anti-scabies vaccine. The purpose of this study is to identify and characterise the sarcoptic allergens. For this purpose, 645 mg of mites, collected from mangy goats, were homogenised in PBS to prepare soluble mite proteins. Fractionation of proteins was initially performed on a Q-sepharose column but the results were unsatisfactory. Consequently, SDS PAGE was used as an alternative. Proteins from the gel were transferred onto a nitrocellulose membrane. The membrane was cut into strips so each strip contained proteins with molecular weights of ³ 90, 80-90, 70-80, 60-70, 50-60, 40-50, 30-40, 25-30, 20-25, 15-20 and 10-15 kDa, respectively. The heat stability of the allergens was determined by heating the suspension at 60ºC for 60 minutes, whereas their dialysability was evaluated using a 10-kDa-cut-off ultramembrane. The activity of the allergens was assayed by an intradermal test on sensitised goats. This study showed that mite protein extract was very potent allergens since mite extract containing as little as 1 ng mite proteins still caused an obvious hypersensitive reaction. The mite extract contained heat-stable, dialysable and non-dialysable allergens. All fractions recovered from a Q-sepharose column contained allergens with almost equal potency. Fractionation with the SDS-PAGE revealed that the allergens had molecular weights of 35 and <10 kDa. The former allergen is assumed to be a member of group 10 allergens, whereas the later belong to haptenic allergens. Kata Kunci: Sarcoptes Scabiei, Allergens, Heat-Stable, Group 10, Hapten

Polymerase chain reaction optimization for the detection of Pasteurella multocida B:2, the causative agent of Haemorrhagic septicaemia Lily Natalia; Adin Priadi
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Specific detection of Pasteurella multocida type B:2 by polymerase chain reaction (PCR), using a set of DNA primers wasoptimised. Effects of the addition of ethylene diamine tetra acetic acid (EDTA) to the sample preparation, Escherichia coli contamination and the number of P. multocida on the PCR product was assessed. The PCR test was compared to the standard bacteriological method for the detection of P. multocida B:2 in tonsillar swab samples collected from slaughter houses of various regions in Indonesia. Addition of 100 mM EDTA-saline to P. multocida B:2 spiked tonsillar swab samples inhibits the production 350 base pairs (bp) PCR product. The inhibitory effect of the EDT A can be eliminated by three times washing with deionised water. The PCR can detect P. multocida as low as I organism and contanimation of 100 CFU of E. coli does not effect the PCR result. The results show that the DNA primers for P. multocida B:2 is sensitive and specific. The inhibitory effect of EDTA in PCR samples can be eliminated by washings. Keywords: PCR, EDT A, Pasteurella multocida B:2

Effect of maturation periods and leukaemia inhibitory factor on in vitro bovine embryo development Endang Tri Margawati
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 3 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The period of in vitro maturation,~20 vs 24 hours) with of without supplementation of :,~uk_ni;ia inhib toryy factor (LIF) (0, 500, 1000 or 2000 U/ml) was studied on bovine embryo development in vitro in a 2 x 4 factotial experiment w id oestgnoo i, a randomized block design . A total of 870 bovine oocytes were used . Besides embryo development, cell numbers of blastocysts v. : ;re also co , mted in order to study the quality of the embryos . Oocytes were matured in a modified TCM199 medium containing 10 ug/nil of FS-14 and LP., I 1~ t1L rstradiol, fertilized in TALP and cultured in SOF/AABSA medium. There was no interaction between maturation periods and LIF doses on embryo development (P>0 .05). Maturation periods, however, affected (P<0.05) blastocyst rates but did not for cleavage o, oocytes and the percentage of oocytes that developed into blastocysts . LIF doses during in vitro maturation did not affect embryo development (P>0 .05) . Cell numbers of blastocysts were also not affected by maturation periods and LIF doses (P<0 .05), however 20 h in vitro maturation and supplementation of LIF doses tended to increase the cell numbers. This study suggests that 20 h maturation increases blastocyst rates and that supplementation with LIF during maturation does not affect the quality of embryos produced in vitro.

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Home Page

OAI Link

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

Abstract

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Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA