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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

97 98 99 100 101

Moleculer analysis of genotype kappa casein and composition of goat milk Etawah grade, Saanen and their crossbred Yayu Zurriyati; R.R. Noor; R.R.A. Maheswari
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Polymorphism of goat casein gene closely linked to the quality of milk protein. κ-casein is one of the casein fractions that influence the shape and stability of grain milk. This study is aimed to identify the variation of genotype κ-casein and related with milk quality from Etawah grade, Saanen and their crossbreed (PE-SA). The number of dairy goats used in this study was 150 animals consisted of Etawah grade (48 animals), Saanen (51 animals) and PE-SA (51 animals). Steps of experiment were: blood and milk sampling collection, DNA amplification by PCR and the product digestion using Pst1 enzyme, κ-casein gene sequencing and analyzing the quality of fresh milk. The results showed that κ-casein gene is monomorphic by PCR-RFLP (Pst1) for all the goat breeds, but DNA sequencing indicated 38 point of mutation. Observation on goat milk quality showed that Etawah grade milk had highest (P < 0.05) density value (1.033 ± 0.002) and solid non fat (9.577 ± 0.704%) than those of Saanen and PE-SA fresh milk goat. Key Words: Dairy Goats, Κ-Casein Gene, PCR-RFLP, Milk

The dry matter digestibility characteristics of tropical tree legumes using menke in vitro gas production technique Dicky Pamungkas; Cesar C Sevilla
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 4 (2003): DECEMBER 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The amount of gas released when a feed was incubated in vitro with rumen fluid, was closely related to the digestibility of the feed. The experiment using Menke in vitro gas production was conducted to determine the dry matter digestibility of tropical tree legumes; Leucaena leucocephala, Flemingia macrophyla, and Gliricida sepium. The samples were collected from Institute of Animal Science farms of the University of the Philippines at Los Banos. The amount of gas produced were recorded at 3, 6, 12, 48 and 72 hours of incubation. To estimate the volume of gas, the equation of Tuah was adopted; while to describe the course of gas production, the equation of MCDONALD was used. The washing loss was not measured and assumed to be zero. Results showed that leucaena at 12 hours was the highest in the volume of gas produced (1.217 ml/g DM). There was a similarity of highly increase of the gas produced between gliricidia and flemingia at 24 hours of incubation i.e 1.60 ml/g DM and 1.60ml/g DM. The potential extent gas production at 72 hours (b value) and the gas production rate (c value) of three legumes were no significantly different. According to the logarithmic-curve of gas volume produced, leucaena tended to be the highest of increase in potential extent and the gas production rate. The different rate of gas production in related to incubation reflected the dry matter digestibility of the three legumes in the rumen. Key words: In vitro gas production, dry matter digestibility

Improvement of native grassland by legumes introduction and tillage techniques Syamsu Bahar; S Hardjosoewignjo; I Kismono; O Haridjaja
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 3 (1999): SEPTEMBER 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Improvement of native grassland by legumes introduction and tillage techniques Improvement of native grassland by legumes introduction and tillage techniques A factorial design using three species of legumes (Siratro, Centro and Stylo) and three different of tillage techniques (no-tillage, minimum tillage and total tillage) was applied in this experiment. The results showed that there was no interaction between species and tillage techniques. There was significant reductions on bulk density from 1.23±0.03 g/cm3 (no-tillage) to 1.07±0.02 g/cm3 (minimum tillage) and 1.05±0.03 g/cm3 (total tillage). Also reductions on penetration resistance from 17.47±3.84 kg/cm2 (no-tillage) to 3.31±0.43 kg/cm2 (minimum tillage) and 3.19±0.45 kg/cm2 (total tillage). Otherwise significant increasing on aeration porosity from 12.80±0.80% vol. (no-tillage) to 21.70±0.95% vol. (minimum tillage) and 20.70±0.35% vol. (total tillage). Total tillage gives increased dry matter yield. Also both total tillage and minimum tillage give yields with a higher percentage of legumes compared with no-tillage. It was concluded that total tillage and minimum tillage could be used for improving native grassland. Key words : Improvement, native grassland, legumes, tillage techniques

Role of seminal plasma in maintaining quality of ram epididymal spermatozoa preserved at low temperature (3–5oC) Muhammad Rizal; Maman Surachman; Herdis .; Achmad Selamet Aku
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 4 (2006): DECEMBER 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Cauda epididymal spermatozoa could be used as an alternative source of gamete in the application of various reproductive technologies, because the spermatozoa is motile and has ability of fertilizing the oocyte. The purpose of this research was to examine the effect of addition of seminal plasma on the quality of ram cauda epididymal spermatozoa preserved at 3–5oC. Collected-spermatozoa was divided into five parts then centrifuged at 3,000 rpm for 30 min and the supernatant was removed. One-fifth was diluted with Tris extender (Tris) and the remaining was added 0.5 ml ram seminal plasma then diluted with Tris extender (PS-Tris), 15% AndroMed + 85% distilled water (PS-AM15), 20% AndroMed + 80% distilled water (PS-AM20), and 25% AndroMed + 75% distilled water (PS-AM25), respectively. Quality of collected-spermatozoa including spermatozoa concentration (SC), percentages of motile spermatozoa (MS), live spermatozoa (LS), abnormal spermatozoa (AS), cytoplasmic droplet (CD), and intact plasma membrane (IPM) were evaluated. Percentages of MS, LS, and IPM of diluted-spermatozoa was evaluated every day during preserved at 3–5oC for three days. Results of this study showed that mean SC, MS, LS, AS, CD, and IPM of fresh spermatozoa were 11,660 million/ml, 65, 81, 7.6, 10.2, and 82.2%, respectively. Addition of seminal plasma in cauda epididymal spermatozoa prior to dilution could maintain the quality of spermatozoa during preserved at 3–5oC for three days. At day-4 of storage, percentages of MS, LS, and IPM for PS-Tris (43, 58, and 59.2%), PS-AM20 (40.5, 53.75, and 53.75%), and PS-AM25 (40, 54.8, and 55.2%) were significantly (P<0.05) higher than Tris (21, 34.8, and 33.6%) and PS-AM15 (20, 40, and 42.2%). In conclusion, addition of seminal plasma in ram cauda epididymal spermatozoa prior to dilution with Tris or 20 and 25% AndroMed extenders could maintain the quality of spermatozoa during preserved at 3–5oC for three days, and could be used for artificial insemination or in vitro embryo production programs. Key Words: Cauda Epididymal Spermatozoa, Seminal Plasma, Tris, AndroMed, Ram

The effect of cryoprotectant and equilibration period on quality and fertility of duck and muscovy sperm A.R Setioko; P Situmorang; E Triwulanningsih; T Sugiarti; D.A Kusumaningrum
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 4 (2002): DECEMBER 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The study was conducted to evaluate the effect of cryoprotectant and equilibration period on quality and fertility of duck and muscovy spermatozoa. Semen collected from Alabio and muscovy drakes was diluted using three different cryoprotectants:glycerol, DMSO and DMF, thereafter the semen was equilibrated 50C for 15; 30 and 60 minutes then frozen and stored in liquid nitrogen, designed by factorial 3 x 3. After thawing, semen sample was investigated on the motility and mortality rate. The best cryoprotectant and equilibration period was used in fertilization test. Duration of fertility was calculated from the second day after insemination until the last fertile egg, and the percent of fertility was calculated from the second day until the forth day after insemination. The use of cryoprotectant significantly affected sperm motility after freezing. The use of glycerol as a cryoprotectant was the lowest (P<0.05) compared to DMSO and DMF. Similarly, duck sperm motility after being freezed with glycerol, DMF and DMSO were 9.02; 21.75, and 32.86%, and for muscovy sperm motility were 11.78; 32.45 and 34.92% respectively. The percentage of live sperm for duck were 23.84; 40.14 and 42.20, while for muscovy were 29.26; 53.06 and 51.80 respectively after being freezed with glycerol, DMF and DMSO. Equilibration period did not affect the percentage of live sperm after freezing. Results of this study showed that duration of fertility of Alabio duck after being inseminated with fresh drake semen was longest compared to that of insemination using fresh muscovy semen, frozen drake semen and frozen muscovy semen (4.96 vs 3.5; 2.4 and 1.5 days respectively). Results from this study clearly indicated that preservation of sperm reduced the quality of spermatozoa. It is suggested that freezing technique of both duck and Muscovy sperm could be conducted using DMF or DMSO as a cryoprotectant with the equilibration period between 15 to 60 minutes. Key words: Sperm, cryoprotectant, fertility, AI, duck, muscovy

Effect of Supplementations of Comin+ and Zn-biocomplex on the performances of Ettawa Crossbred goats Supriyati .; W Puastuti; I-G.M Budiarsana; I-K Sutama
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 4 (2012): DECEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Digestibility of ruminant feeds is greatly determined by ruminal fermentation which in turn is greatly affected by micro-ecology of the rumen. Manipulation of micro-ecology such as supplementing with various yeasts or micro elements could improve digestibility of the feed. The effect of Comin+ (R1) and Zn-biocomplex (R2) alone or their combination (R3) on performance of Ettawa Crossbred kids, fed concentrate containing Aspergillus oryzae (R0) was studied. Twenty four Ettawa Crossbred kids (6-8 month of age; initial liveweights =16.63±2.10 kg) were divided into 4 groups. Feeds given were King grass (Pennicetum purpuroides) ad libitum and concentrate. The observations were carried out for 16 weeks with 2 weeks adaptation period and the experiment was conducted in a completely randomized design with 4 treatments and 6 replications. The results showed that supplementations significantly (P < 0.05) increased total DM intakes from 685 g/d (R0) became 748, 711 and 858 g/d; and grass DM intakes from 304 g/d (R0) became 373, 331 and 479 g/d for R1, R2 and R3, respectively. Digestibilities of DM, NDF and DE/GE were not affected by supplementation, but supplementations significantly (P < 0.05) affected digestibilities of ADF and CP. Supplementation also significantly improved (P < 0.05) ADG from 65.18 g/d (R0) became 94.64, 83.04 and 90.77 g/d; and FCR from 10.51 (R0) became 7.82, 8.36 and 9.46 for R1, R2 and R3, respectively. It was concluded that supplementation of Comin+ (R1) gave the best growth performance of Ettawa Croosbred goats, where ADG and FCR improved by 45.20% and 34.40%. Key Words: Supplementation, Comin+ , Zn-biocomplex, Performances, Goats

Isolation of Pasteurella multocida from broiler chickens Sri Poernomo; A Sarosa
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 2 (1996)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Pasteurella multocida, the etiological agent of fowl cholera, was isolated from five, 32 days oldbroilerchickens in the late of 1992. The chickens were from a farm located in Bogor area, raised in cages and each flock consisted of 1,550 broilers . Therewere 230 birds, aging from 28-31 days old, died with clinical signs of lameness and difficulty in breathing. Serological test of the isolate revealed serotype Aof Carter classification . To prove its virulences, the isolate was then inoculated into 3 mice subcutaneously. The mice died less then 24 hours postinoculation and P. multocida can be reisolated . The sensitivity test to antibiotics and sulfa preparations showed that the isolate was sensitive to ampicillin, doxycyclin, erythromycin, gentamycin, sulfamethoxazol-trimethoprim and baytril, but resistance to tetracyclin, kanamycin and oxytetracyclin. This is the first report of P. multocida isolation in broiler chickens in Indonesia, and it is intended to add information on bacterial diseases in poultry in Indonesia. Keywords : Pasteurella multocida, isolation, broiler chickens, antibiotics

Effects of concentration of Follicle Stimulating Hormone (FSH) on the rates of ovulations and twinning birth Polmer Situmorang; R Sianturi; D. A Kusumaningrum; E Triwulaningsih
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 4 (2010): DECEMBER 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Twinning in monotocous domestic species dairy cattle is most frequently by dyzygote in which two eggs is ovulated by treatment with gonadotropin hormon. A study to see the effects of concentration of Follicle Stimulating Hormone (Folltropin) on the ovulation rates and twinning birth was conducted. The experiment designed was commpletely randomized designed with 3 treatment of total concentration of FSH (A: 12, B: 6 and C 3 ml) and 4 lactating cows for a replications. Hormon was intramussculary injected in decreasing dossis method twice a day with 12 hours apart for 4 days. Data recorded were the diameter of ovary (DO), total corpus luteum (TCL), concentration of progesterone on the day 12 of estrus cycle (P), percentage of pregnancy and number of birth. FSH significantly increased the ovulation rates. The means DO was significantly (P < 0.05) higher after injection (6.3 cm) than those before injection (2.0 cm). The TCL and P were highly significant (P < 0.01) higher after injection. The TCL and P were 1.2 and 0.8 ng/ml and 8.5 and 3.0 ng/ml before and after hormone treatment respectively. Concentration of FSH significantly increase (P < 0.05) the TCL and P but not for DO. The means DO, TCL and P were 5.0, 4.5 and 1.6, 5.5, 8,8 and 1.8 and 8.4cm, 12.3 and 5.3 ng/ml for treatments C, B and A respectively. Persentage of pregnancy was decreasing by the increasing of ovulations rates and the highest percentage of pregnancy 75% was obtained on treatments C. The normal single birth was found for treatment C and neonatal death were occurred for treatment B and A where the number of calves is higher than 2. In conclusion the FSH increased the ovulation rates in dairy cattle and the ovulation rates obtained was also affected by the concentration of hormone. Key Words: Hormone, FSH, Ovulation, Twin Birth

Comparison between antibody-elisa test and fecal egg count for detecting Fasciola gigantica infection in cattle S Endah Estuningsih; S Widjajanti; Gatot Adiwinata
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 1 (2004): MARCH 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The comparison between antibody-ELISA test and fecal egg count for detection of natural infection of Fasciola gigantica in cattle was observed. One hundred and fifty samples of blood, feces and livers were collected from cattle slaughtered in the abattoir in Jakarta. Serum was collected from the blood samples and the level of antibody was determined by using antibody- ELISA test. The fecal samples were processed by using sedimentation technique in order to detect the present of F. Gigantica eggs. The livers were processed for liver flukes count. The result showed that from the liver examination, 38.7% cattle were negative flukes, 16% had 1-10 flukes, 34% cattle had 11-100 flukes and 11.3% cattle had more than 100 flukes. About 44.7% infected cattle had less than 100 eggs of F. gigantica per gram feces, however no eggs of F. gigantica were found in 13% infected cattle. The result of antibody-ELISA test showed that from 92 cattle infected with F. gigantica, 84 cattle had OD > 0.38 (range from 0.38-1.77) and 8 cattle had OD < 0.38 (range from 0.18-0.37). In contrast, from 58 cattle without flukes, 7 cattle had OD > 0.38 (range from 0.38-1.95) and 51 cattle with the OD < 0.38 (range from 0.1-0.33). The sensitivity of the fecal examination technique was 87% and the specificity was 100%. The sensitivity and specificity for antibody-ELISA test were 91 and 88% respectively. Key words: Antibody-ELISA, Fasciola gigantica, feces, liver

To trace the active compound in mengkudu (morinda citrifolia) with anthelmintic acvtivity against Haemonchus contortus T.B Murdiati; G Adiwinatai; D Hiladsari
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 4 (2000): DECEMBER 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Intestinal parasites such Haemonchus contortus is usually controlled by management improvement and regular administration of anthelmintic. However, there is an indication of H. contortus resistance to several anthelmintic available in the market, which makes medicinal plants as an alternative anthelmintic and mengkudu or noni fruit (Morinda citrifolia) have been reported as an effective anthelmintic. To trace the active compounds responsible for anthelmintic activity against H. contortus, the mengkudu fruit was continuosly extracted into hexane, chloroform, metanol and water, followed by in-vitro study on the anthelmintic activity. The in-vitro anthelmintic activity was base on the ability of the extracts to kill the worm and the ability of the extracts to prevent egg development. The study suggested that chloroform fraction which contains alkaloid and anthraquinon have the highest anthelmintic activity and showed significant different compared to control (P≤ 0.05). Key words: Morinda citrifolia, anthelmintic, Haemonchus contortus

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Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

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Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

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Location
Kota bogor,

Jawa barat

INDONESIA