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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 7 Documents
 1 
Search results for , issue "Vol 49, No 3 (2024): September" : 7 Documents clear
Electroencephalogram, creatine kinase, and lactate dehydrogenase responses during preslaughter handling in goats handled by trained and untrained handlers Kumar, P.; Abubakar, A. A.; Adewale, M. A.; Hayat, M. N.; Adamu, G.; Ajat, M.; Goh, Y-M.; Kaka, U.; Sazili, A. Q.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 3 (2024): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.3.215-223

Abstract

The present study evaluated the effect of training to livestock handlers on electroencephalogram (EEG) and plasma enzymes levels during preslaughter handling in goats. Six handlers were divided into three groups viz., trained (T-trained in basic animal handling practices, animal behavior, and animal welfare principles), contact trained (CT-not trained directly but interacted and saw the working of trained handlers), and untrained (UT-no formal training). Eighteen Boer cross bucks were used in the study by following a cross-over design and electroencephalogram spectrum, creatine kinase (CK), and lactate dehydrogenase (LDH) concentrations were recorded before handling (in lairage) and after handling (at slaughter point). The training of livestock handlers had a significant (p<0.05) effect on the total power (Ptot) and median frequency (MF) of the EEG spectrum. Whereas no significant (p>0.05) change was recorded in the alpha, beta, delta, theta, and gamma bands activity. The goats handled by trained handlers exhibited significantly (p<0.05) lower CK and LDH concentrations than goats handled by CT and UT handlers, thereby indicating higher preslaughter stress in goats handled by CT and UT groups. Therefore, the current study emphasizes the significance of providing training to livestock handlers in order to mitigate preslaughter stress levels in goats.
The potency of sericin as an alternative protein in collection and maturation media to support in vitro bovine oocyte maturation Karja, N. W. K.; Satrio, F. A.; Setiyono, A.; Prasetyaningtyas, W. E.; Fahrudin, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 3 (2024): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.3.224-230

Abstract

The purpose of this study was to evaluate the potency of sericin as a substitute for BSA (bovine serum albumin) to support the in vitro maturation of bovine oocytes. Cumulus-oocytes complexes were collected with BSA or 0,1% sericin, and then matured either in media with BSA or sericin. The maturation rate was evaluated based on the meiotic status of oocytes. The incidence of DNA fragmentation in oocytes was assessed by TUNEL. The percentage of oocytes reaching the MII stage in the media either with BSA or sericin was significantly higher than those of oocytes collected and matured in BSA only. Although the maturation rate of oocytes collected with BSA and then matured with sericin was comparable to oocytes in BSA-BSA groups (P>0.05) the rate was similar to oocytes collected with sericin and then even matured with BSA or sericin (P>0.05). There was no significant difference in the incidence of DNA fragmentation among the treatment groups (P>0.05), the indices of DNAfragmented oocytes were found around 17-20 %. In conclusion, Sericin has the potency to replace BSA as a source of protein either in collection media and/or in maturation media as well as potentially preventing the incidence of DNA fragmentation in oocytes.
The reduction of fat deposition in broiler chickens fed diet containing modified rice bran tempeh Santoso, U.; Fenita, Y.; Kususiyah, K.; Fahmi, Z. O.; Restu, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 3 (2024): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.3.231-238

Abstract

The aim of this study was to assess the impact of modified rice bran tempeh on performance, carcass quality, organoleptic properties, fat deposition, and nutritional meat composition in broiler. This research used a completely randomized design. Two hundred broilers (a male to female ratio of 1:1) were divided into 4 treatment groups with 5 replications for each treatment, as follows: P1 = Feed without modified rice bran tempeh; P2 = Feed with 20% rice bran tempeh; P3 = Feed with 20% modified rice bran tempeh; and; P4 = Feed with 25% modified rice bran tempeh. The experimental results showed that all treatment groups had similar performance. The treatments significantly reduced (P<0.05) abdominal fat and total fat deposition. In addition, the treatments also significantly reduced meat fat and ash content (P<0.01), but it significantly increased (P<0.01) moisture contents. It was concluded that feeding 20% or 25% modified rice bran tempeh reduced meat fat content and fat deposition without reducing broiler performance.
Fat supplementation containing high palmitic acid enriched with lecithin on the performance, egg quality, and fatty acid profile of quail Yano, A. A.; Hermana, W.; Retnani, Y.; Syarif, I.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 3 (2024): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.3.239-251

Abstract

This study examined the effects of fat supplementation with high palmitic acid enriched with lecithin on the performance, egg quality, and fatty acid profile of quails. This study applied a completely randomized design to 330 female quails (Cortunix cortunix japonica) which were allocated into 5 different groups with 3 replicates of 22 birds. The groups were assigned as follows: T0 = commercial diet (control), T1 = commercial diet + 2.5% fat + 6% lecithin of added fat, T2 = commercial diet + 2.5% commercial fat, T3 = commercial diet + 5% fat + 6 % lecithin of added fat, T4 = commercial diet + 5% commercial fat. The results showed that the treatment group did not give effects on performances. However, the treatment group with fat level of 2.5% and 5% increased the score of egg yolk and eggshell thickness. Moreover, the treatment group decreased fat content in terms of saturated fatty acids and n-6/n-3 ratio of quail eggs. In conclusion, fat supplementation containing high palmitic acid enriched with lecithin proves effective in improving egg quality. However, the performance remains relatively similar across the groups.
Association of prolactin gene polymorphism with milk production traits in Gaolao cattle Kale, D. S.; Singh, J.; Sathe, Y. B.; Wankhade, A.; Dudule, P. D.; Patil, D. V.; Gowane, G. R.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 3 (2024): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.3.193-203

Abstract

The present study aimed to identify the DNA polymorphisms within prolactin gene regions of Gaolao cattle and to investigate their relation with milk production traits. The genomic DNA was isolated from 245 unrelated animals of Gaolao cows, and test-day milk traits data were recorded. PCRRFLP, PCR-SSCP and direct sequencing methods for mutation confirmation were used to study polymorphism within the prolactin gene. The PCR-RFLP genotyping at the exon-3 region of the prolactin gene revealed polymorphism and found an association of the PRLG1-RsaI ‘AA’ genotype with milk yield (5.05±0.14*) in Gaolao cattle. The exon-4, intron-3,4, Exon-5, 5'flanking, and exon-1 regions of prolactin revealed SSCP polymorphisms and SNPs. The results of the study indicate the existence of a substantial amount of genetic variation within the above-studied regions of the prolactin candidate gene. The identified association at PRLG1-RsaI genotype with milk yield will aid in future geneassisted selection and improvement strategies in Gaolao indigenous cattle.
Effect of rumen-protected fat on in vitro rumen fermentation and apparent biohydrogenation of fatty acids Behan, A. A.; Chwen, L. T.; Kaka, U.; Muhammad, A. I.; Samsudin, A. A.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 3 (2024): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.3.252-263

Abstract

This study aimed to evaluate the effects of rumen-protected fat (RPF) on in vitro fermentation profiles and biohydrogenation of fatty acids. The treatment diets were basal diet (70:30 concentrate to rice straw) with no RPF (CON), basal diet plus prilled fat (PF), basal diet plus prilled fat with lecithin (PFL) and basal diet plus calcium soap of palm fatty acids (CaS). In vitro gas production, fermentation kinetics, in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), metabolizable energy (ME), rumen fermentation and fatty acid profile were determined. The results show that RPF did not affect cumulative gas production and gas production kinetics. PFL significantly (p < 0.05) improved IVDMD and IVOMD, although the addition of RPF did not affect ME. The volatile fatty acid (VFA), pH, ammonia nitrogen, methane, and molar proportion of VFA were not significantly influenced by the RPF; methane was numerically reduced because of PFL treatment. The concentration of monounsaturated and polyunsaturated fatty acids increased (p < 0.05) whereas that of saturated fatty acids decreased in the control diet. The biohydrogenation of C18:2n-6 and C18 unsaturated fatty acids was enhanced (p < 0.05) by PFL. These findings suggest that PFL enhances gas production, decreases methane and increases the biohydrogenation of C18:2n6 without disrupting rumen fermentation.
Association of CYP2A6 gene related to the characteristic carcass, commercial cuts, quality of meat and cholesterol of lamb meat Istiqlal, R. F.; Listyarini, K.; Jakaria, J.; Budiman, C.; Gunawan, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 3 (2024): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.3.204-214

Abstract

As meat-producing livestock, sheep are a benchmark for communities seeking quality and healthy meat. The CYP2A6 gene has emerged as a potential tool for improving lamb meat quality. This study investigated the genetic variability of the CYP2A6 gene and its association with key determinants of lamb meat quality. The research involved 140 male sheep from five breeds: Javanese thin-tail sheep (JTTS), Garut sheep (GS), Jonggol sheep (JS), Barbados cross sheep (BCS), and Compass agrinak sheep (CAS). PCR-RFLP was employed to identify CYP2A6|BsmAI gene polymorphism and the general linear model (GLM) was used to analyze the gene's association with meat quality. The results showed a polymorphism in the CYP2A6 gene (SNP g.49170107 G>T), presenting two genotypes: GG and GT. The analysis results demonstrated that the CYP2A6 gene (P<0.05) significantly correlates with carcass characteristics (live weight, hot carcass weight, carcass percentage, cold carcass weight), commercial cuts, physical meat quality, and cholesterol content. The GT genotype exhibited superior meat quality to the GG genotype, suggesting that the CYP2A6 gene could serve as a valuable genetic marker for enhancing lamb meat quality.

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