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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 676 Documents
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OVARIAN RESPONSE OF DAIRY COWS TO PROGESTERONE COMBINED ON Toleng, A.L.; Yusuf, M.; Hasbi, H.; Putri, A.R.M.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 3 (2013): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.3.156-162

Abstract

The objective of this study was to evaluate the response of ovaries in estrus synchronizationprotocol using GnRH-PGF2α based protocol combined progestin in a herd with tie-stall housing system.A total of 20 Holstein Friesian cows at different stages of estrus cycle in a dairy herd were enrolled inthe present study. The cows were randomly allocated into two treatment groups; with and without CIDR(Controlled internal drug release) insertion. All cows received 100 μg of GnRH I.M. at the beginning ofthe treatment (d 0) without regard to the stages of the estrus cycle followed by 25 mg PGF2α IM 7dayslater. Cows in CIDR group were inserted CIDR into the vagina at the time of GnRH administration andwere removed on the day of PGF2α administration. estrus signs were checked twice daily starting onday-2 after initiation of the protocol, except cows in CIDR group the estrus signs were checked startingon day-8 of the protocol. The animals showing estrus signs were noted. Blood samples were collectedfrom all animals on the days of GnRH and PGF2α treatments and on day-10 after initiation of protocolfor progesterone concentration. The results of this study showed that 30% animals in cows withoutCIDR insertion showed estrus signs prior to PGF2α injection. Involving CIDR to synchronized estruswith GnRH-PGF2α based protocol avoided the occurrence of premature estrus and maintained highprogesterone concentration on the day of PGF2α administration. It is concluded that ovarian responseafter involving CIDR in the protocol for estrus synchronization especially using combination of GnRHand PGF2α were much effective to synchronize the initiation of estrus in dairy cows. Likewise, the useof progesterone avoided premature estrus.
Polymorphism identification of Diacylglycerol Acyltransferase1 gene and its correlation with fat content of Saanen goat Darmoatmodjo, L. M. Y. D.; Widodo, W.; Asmara, W.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.211-217

Abstract

Gene markers are often usefull for selection process of cattle on commercial certain phenotypes. Saanen goat has predominance in milk production traits. Milk fat content is one of the commercial traits of milk production. Diacylglycerol Acyltransferase1 (DGAT1) gene was involved in the final process of triglyceride synthesis and associated with milk fat content. Single nucleotide subtitution or insertion-deletion in certain sequence of DGAT1 gene could be gene markers for milk fat content. The objective of this study was to identify differences variation in milk fat content among individual Saanen goats, to identify exon 14-16 polymorphism of DGAT1 gene and to examine its correlation between polymorphism and milk fat content on Saanen goats. The methodologies was collection blood and milk samples, DNA isolation from blood, amplification of DGAT1 genes, sequencing, detection of polymorphism of exon 14-16 DGAT1 gene with Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP), and examination milk quality on three lactation months using Lactoscan. The average of daily milk production from 70 Saanen goat was 2340±709 mL, 1880±632 mL, 1432±536 mL and average of milk fat content was 3.07±0.52%, 2.53±0.55%, 3.00±0.55% for the first, second and third months of lactation, respectively. The amplicon size of exon 14-16 of DGAT1 gene was ± 350 bp and showed monomorphic pattern from PCR-SSCP. Variation of milk fat content on Saanen goats not caused by polymorphism of exon 14-16 DGAT1 gene.
THE USE OF Pomacea canaliculata SNAILS IN FEED TO IMPROVE QUALITY OF ALABIO DUCK (Anas plathyrinchos Borneo) MEAT Subhan, A.; Yuwanta, T.; Zuprizal, Z.; Supadmo, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 4 (2015): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.4.238-244

Abstract

This research was aimed to improve the physical and chemical quality of Alabio ducks which was fed with Pomacea canaliculata snails. Those ducks were raised intensively. There were nine treatments  included R0 (control feed), R1 (control feed + 2.5% Pomacea canaliculata snails from swampy area), R2 (control feed + 5% Pomacea canaliculata snails from swampy area), R3 (control feed + 7.5% Pomacea canaliculata snails from swampy area) R4 (control feed + 10% Pomacea canaliculata snails from swampy area) R5 (control feed + 2.5% Pomacea canaliculata snails from tidal swampy area), R6 (control feed + 5% Pomacea canaliculata snails from tidal swampy area, R7 (control feed + 7.5% Pomacea canaliculata snails from tidal swampy area, and R8 (control feed + 10% Pomacea canaliculata snails from tidal swampy area. The variables observed included meat chemical and physical quality. A Completely Randomized Design was used in this study. Analysis of variance and Duncan’s multiple range test were used to analyze data. The research results revealed that using Pomacea canaliculata snails in duck feed had a significant effect (P<0.05) towards the physical characteristics (water holding capacity, cooking loss, and tenderness), and chemical characteristics of Alabio duck meat (water, protein, collagen, fat, and cholesterol content). However, there was no significant effect towards meat pH. It can be concluded that using 5% Pomacea canaliculata snails in a mixture of Alabio duck feed decreased cooking loss and meat cholesterol content.
RUMINAL FERMENTATION KINETICS OF MORINGA AND PELTIPHYLLUM SUPPLEMENTS DURING EARLY INCUBATION PERIOD IN THE IN VITRO READING PRESSURE TECHNIQUE Jayanegara, A.; Sabhan, T.; Takyi, A.K.; Salih, A.O.; Hoffmann, E.M.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 3 (2010): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.3.165-171

Abstract

This experiment was aimed to observe rumen fermentation kinetics of alternative supplements, i.e.Moringa oleifera and Peltiphyllum peltatum leaves added to maize silage diet as compared to aconventional supplement (barley-soya). A total of six treatments were investigated in the present study,which consisted of: maize silage (A), M. oleifera (B), P. peltatum (C), maize silage + concentrate (56:44,w/w; D), maize silage + M. oleifera (56:44, w/w; E), and maize silage + concentrate + P. peltatum(47:37:16, w/w/w; F). The feeds were incubated in vitro in three replicates (completely randomized)using the Reading Pressure Technique system. Approximately 800 mg of the feeds were mixed by 75 mlof buffered rumen liquor. The incubation was carried out up to 12 h in bottles and gas pressure wasrecorded and released in regular intervals. Repeated sampling was conducted for analysis offermentation products at 1, 6 and 12 h of incubation period. The results showed that the nutritionalquality of Moringa and its fermentation pattern was comparable to that of barley-soya concentrate. PlainMoringa incubation resulted the highest production of iso-SCFA and soluble protein concentrationamong all treatments after 12 h incubation (P<0.05). Incubation of sole Peltiphyllum peltatum highlyhampered the fermentation rate. Production of iso-SCFA both for plain Peltiphyllum and its mixturewere comparatively low. Supplementation of Peltiphyllum increased significantly soluble proteinconcentration during 12 h incubation (P<0.05). Peltiphyllum also had a very low C2+C4/C3 ratiocompared to other treatments. It could be concluded that Moringa oleifera is a potential alternativesupplement to replace either partially or completely concentrate as a conventional supplement, andPeltiphyllum peltatum supplementation could reduce excessive protein degradation and fermentation ofthe concentrate in the rumen.
Study of physic and organoleptic of butter developed using milk from cow and goat reared in Sleman Regency, Yogyakarta, Indonesia Murti, T. W.; Pradana, M. W. E.; Nurasri, A. D.; Arlinda, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 4 (2020): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.4.338-347

Abstract

This study was to see physic-organoleptic of butter from goat as compared to cow butter reared in the Mt Merapi, Sleman regency, Yogyakarta. Both butter has been evaluated their fat size and numbers, the value of hardness, melting point, spreading and organoleptic performance. Fat size and numbers have evaluated microscopically, hardness by penetrometer, butter melting point at different temperature 30, 40, and 50 oC, as well as spreading by pressing down with ± 300 gram of weights. Organoleptic performances have been evaluated by panelist. The result of both butter were good. Goat butter contained more number of small-size fat globule lead to softer butter with hardness 0.3 kg/cm2 than cow butter with hardness more than 1.0 kg/cm2. Goat butter have melted faster than cow butter. Cow butter spreading were less rapid with 6 minutes than 1.5 minutes of goat butter. Panelist acceptance of cow butter were bigger than goat butter. It has been concluded that cow butter have had physically hard performance and well accepted than goat butter. Goat butter have presented well as fresh butter than storage butter.
MORPHOLOGY AND GENETIC DIVERSITY OF MITOCHONDRIAL DNA D-LOOP REGION USING PCR-RFLP ANALYSIS IN MAGELANG DUCK AND OTHER NATIVE DUCK Purwantini, D.; Yuwanta, T.; Hartatik, T.; Ismoyowati, I.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 1 (2013): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.1.1-9

Abstract

The aim of this study was to investigate the different of plumage colors on morphological diversityof Magelang duck and genetic diversity using PCR-RFLP mtDNA D-loop region analysis of Magelangduck and four others native duck population (Tegal, Mojosari, Bali and Alabio duck) in Indonesia. Bloodsample was taken from 50 Magelang ducks and 20 of each native ducks. Morphological characteristicsof body measurement, production ability and egg quality of Magelang duck were analyzed usingCompletely Randomized Design with 11 plumage colors as treatments. PCR technique was administeredto amplify fragments in mtDNA D-loop region and PCR products were digested with endonucleaserestriction enzyme AluI and HaeIII. The result showed that morphology diversity of Magelang duck wasstatistically affected by different plumage colors. PCR-RFLP analysis using AluI and HaeIII restrictionenzyme resulted in six combinations of restriction fragment pattern shown in six haplotypes (A, B, C, D,E and F). Haplotype difference showed genetic diversity in the population of Magelang duck and theother native ducks. In conclusion, the different plumage colors affected morphology diversity ofMagelang duck. Genetic diversity of Indonesian native duck population could be identified by usingPCR-RFLP analysis on mtDNA D-loop region.
Genetic polymorphism of Pit-1|HinfI gene in Grati-Ongole Grade cattle at Indonesian Beef Cattle Research Station Hartati, H.; Anwar, S.; Soewandi, B.D.P.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.315-322

Abstract

The pituitary transcription factor (Pit-1) gene is one of the considered genes that responsible to growth in cattle. A specific Pit-1|HinfI gene polymorphism located within exon 6(g.1256G>A) has been shown to be associated with growth and milk traits in cattle. This study aimed to identify the Pit-1|Hinf1 gene polymorphism in Grati-Ongole Grade cattle (Grati-OG cattle). A total of 107 genomic DNA of Grati-OG cattle were used in this study. The detection of polymorphism was performed by PCR-RFLP method. Only AB and BB genotypes were succesfully identified with the frequency of 0.009 and 0.991, respectively. Whereas, the frequency of A and B alleles were 0.005 and 0.995, respectively. The observed genotype frequencies in this population do not deviated from HWE. The value of Ho, He and PIC were 0.009, 0.009 and 0.009, respectively.In conclusion, the Pit-1|HinfI gene polymorphism in Grati-OG cattle is monomorphic and hence it should not be used in further association studies.
LEAF QUALITY AND YIELD OF Gliricidia sepium (Jacq) Steud UNDER DIFFERENT POPULATION DENSITY AND CUTTING INTERVAL IN COCONUT PLANTATION Anis, S. D.; Kaligis, D. A.; Tulung, B.; Aryanto, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 2 (2016): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.2.91-98

Abstract

The objective of this study was to evaluate leaf quality of Gliricidia sepium (Jacq) Steud and vegetative growth under different population density and cutting interval in coconut plantation. Plant material of 30 cm length with diameter of 2-3 cm were planted in the poly-bag of 15 x 25 cm size. The area were fertilized using Triple Super Phosphate (TSP) and Kalium Cloride (KCl) fertilizer at 75 kg/ha during land  plough and using N fertilizer (Urea) at 100 kg/ha applied at two months age of plant. Treatment of population density of Gliricidia sepium (Jacq) Steud at 20, 30 and 40 plants per area of 9 m2 combined with cutting interval of 3; 6; 9 and 12 weeks were conducted in this study. Treatments were set in factorial arrangement based on block randomized design. Variables measured were including quality of leaf, dried matter production rate and vegetative growth. Results showed that nutrient content of the leaf of Gliricidia sepium (Jacq) Steud dictated by cutting interval, and vegetative performance influenced by the interaction of population density and cutting interval. In conclusion, to sustain high dry matter leaf production and quality of Gliricidia sepium (Jacq) Steud underneath coconut plantation management should be focused on the planting and defoliation regimes. 
FEASIBILITY STUDY OF ESTABLISHING AN ARTIFICIAL INSEMINATION (AI) CENTER FOR CARABAOS IN SAN ILDEFONSO, BULACAN, PHILIPPINES Arrienda II, F.Q.; Cruz, C.V.; Perilla, M.V.; Setiawan, B.M.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 2 (2010): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.2.134-141

Abstract

The productivity of the carabao subsector is influenced by several constraints such as social,technical, economic and policy factors. The need to enhance the local production of carabaos will helplocal farmers to increase their income. Thus, producing thorough breeds of carabaos and improving itgenetically is the best response to these constraints. This study was conducted to present the feasibilitystudy of establishing an Artificial Insemination (AI) Center and its planned area of operation in Brgy.San Juan, Ildefonso, Bulacan. The market, production, organizational and financial viability of operatingthe business would also be evaluated. This particular study will provide insights in establishing an AICenter. Included in this study is the identification of anticipated problems that could affect the businessand recommendation of specific courses of action to counteract these possible problems. Primary datawere obtained through interviews with key informants from the Philippine. Carabao Center (PCC). Togain insights about the present status of an AI Center, interviews with the technicians of PCC and privatefarm were done to get additional information. Secondary data were acquired from various literatures andfrom San Ildefonso Municipal Office. The proposed area would be 1,500 square meters that would beallotted for the laboratory and bullpen. The AI Center will operate six days a week and will be openedfrom 8 AM until 5 PM. However, customers or farmers can call the technicians beyond the office hoursin case of emergency. The total initial investment of Php 3,825,417.39 is needed in establishing the AICenter. The whole amount will be sourced from the owner’s equity. Financial projection showed an IRRof 30% with a computed NPV of Php 2,415,597.00 and a payback period of 3.97 years. Based on all themarket, technical, organizational, financial factors, projections and data analysis, it is said that thisbusiness endeavor is viable and feasible.
SCREENING APPROACHES FOR METHANE MITIGATING POTENTIAL OF TANNIN-CONTAINING PLANTS UNDER IN VITRO RUMEN ENVIRONMENT Jayanegara, A.; Ridla, M.; Nahrowi, N.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 4 (2012): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.4.278-285

Abstract

The aim of the present study was to conduct univariate, bivariate and multivariate (principalcomponent analysis, PCA) approaches in the screening of tannin-containing plants from variouscollection sites for their CH4 mitigating properties. Plant samples were obtained from various collectionsites in different countries, i.e. Indonesia (n = 27 species), Mongolia (n = 14), Switzerland (n = 16) andGermany (n = 3). The plants were incubated in vitro with buffered-rumen fluid at 39oC for 24 h. Totalgas production was recorded as an indicator of feed quality and emission of CH4 was measured. Resultsshowed that, based on bivariate screening, generally, plants possessed low CH4 production had lowquality or low total gas production except Rhus typhina, i.e. 43 ml/200 mg DM. The loading plot of PCAshowed that all phenolic fractions were in the opposite direction with CH4 and total gas production.Plants clustered together in reverse direction to that of CH4 were Bergenia crassifolia root and leaf,Swietenia mahagoni, Clidemia hirta, Peltiphyllum peltatum, Acacia villosa and R. typhina. It wasconluded that, for tannin-containing plants, screenings based on univariate, bivariate and multivariateapproaches in relation to ruminal CH4 emission led to similar results.

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