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Journal of Biomedicine and Translational Research
Published by Universitas Diponegoro
ISSN : -     EISSN : 25032178     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Journal of Biomedicine and Translational Research (JBTR) is an open access, international peer-reviewed journal that considers articles on: clinical medicine, molecular medicine, tropical medicine, infectious diseases, cardiovascular medicine, molecular biology, genetics, immunology, microbiology, biochemistry, and pharmacotherapy with particular interest on the link between clinical and basic research called translational research.
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Articles 8 Documents
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Search results for , issue "Vol 7, No 2 (2021): August 2021" : 8 Documents clear
Correlation between Non-Alcoholic Fatty Liver Disease (NAFLD) fibrosis score (NFS) with Left Ventricular Mass Index (LVMI) in patients with NAFLD Tri Ferry Rachmatullah; Cecilia Oktaria Permatadewi; Hesti Triwahyu Hutami; Charles Limantoro; Hery Djagat Purnomo, MD, PhD
Journal of Biomedicine and Translational Research Vol 7, No 2 (2021): August 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i2.11624

Abstract

Background: Cardiovascular-related mortality is a major concern in NAFLD. Advanced fibrosis was known to be associated with cardiovascular diseases. NAFLD Fibrosis Score (NFS) is used to identify the development of liver fibrosis in NAFLD patients. Left ventricular mass index (LVMI) is a sign of subclinical cardiovascular complications in NAFLD. The correlation between NAFLD fibrosis score with LVMI in NAFLD patients is not fully established.Objective: To analyze the correlation between NAFLD fibrosis score with LVMI  in NAFLD patients.Methods: A cross-sectional study of NAFLD patients in Kariadi Hospital Indonesia. NFS was done using a formula based on clinical and biochemical parameters. LVMI was measured with echocardiography. Pearson’s, Mann-Whitney, and logistic regression were used for analysis.Results: A total of 64 patients with primary NAFLD were enrolled, 54.7% males and 45.3% females. Mean age was 52,8 ± 10,5 years (30-77 years). Based on NFS criteria, high probability group was the highest (50%), followed by intermediate probability group (34,4%) and low probability group (15,6%). Highest increase in LVMI was obtained in the high probability group (93,8%), followed by intermediate probability (59,1%), and low probability group (10%) respectively. There was significant correlation between NFS and LVMI (P 0,002). Logistic regression showed that NFS has a more significant correlation with LVMI compared to gender (P=0,002).Conclusion: NFS is a non‐invasive liver fibrosis scores which independently corelated with Left ventricular mass index (LVMI), a marker of cardiovascular abnormality.
The Association between Asphyxia and Interleukin (IL)-6 and IL-1β Levels in Neonates Nenden Nursyamsi Agustina; Muhammad Sholeh Kosim
Journal of Biomedicine and Translational Research Vol 7, No 2 (2021): August 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i2.11185

Abstract

Background: Neonatal asphyxia is a respiratory failure during and just after birth. It can cause morbidity and mortality in neonates.Interleukin (IL)-6 and IL-1β are inflammatory cytokines produced by neuronal cells in early response to brain injury due to asphyxia. However, their role in neonatal asphyxiais remain elusive.Objective: To determine theassociation between asphyxia and serumIL-6 and IL-1β levelsin neonates.Methods: Across-sectional study was conducted on neonates diagnosed with moderate to severe asphyxia who hospitalized atthe Dr. Kariadi General Hospital Semarang Indonesia from December 2013 to May 2014. The subjects were examined for serum IL-6 and IL-1β. Blood samples were obtained from umbilical vein in the first 24 hours of life. Serum IL-6 and IL-1β levelswere measured using immunoassay. Dependent variable were IL-6 and IL-1β level. Bivariate analysis was performed using chi-square test, for the assessment of the association between dependent and independent variables. A p-value of less than 0.05 was considered statistically significant.Result: A total of 54 subjectswere enrolled in this study. No significant difference between moderate and severe asphyxia neonatesin term ofsex, birthweight,type of delivery, neonate’s mother age, gestational age, and parity. Levels of IL-6 and IL-1β levels wereincreased significantly in both moderate and severe asphyxiagroups, and the levels were significant higher in the severe asphyxia than that of in the moderate, p=0.003 and p=0.007, respectively.Conclusion:There was association between asphyxia and IL-6 and IL-1β levelsin neonates.IL-6 and IL-1β levelswere increased in neonates with moderate and severe asphyxia, with extend of increase was significant higherin the later.    
Adiponutrin and Adiponectin Gene Variants in Indonesian Patients with Non-Alcoholic Fatty Liver Disease: a Preliminary Study Rayvita AN Meagratia; Ferdy Kurniawan Cayami; Udin Bahrudin; Wiwik Lestari; Nani Maharani; Sultana MH Faradz; Hery Djagat Purnomo
Journal of Biomedicine and Translational Research Vol 7, No 2 (2021): August 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i2.11777

Abstract

BackgroundVariants of adiponutrin (PNPLA3) and adiponectin (ADIPOQ) genes were considered to be associated with non-alcoholic fatty liver disease (NAFLD). Although the prevalence of NAFLD is increasing, there are limited numbers of studies about the association in Indonesian population.ObjectiveTo confirm whether specific variants of adiponutrin (PNPLA3) and adiponectin (ADIPOQ) genes are associated with NAFLD in Indonesian patients.MethodsData and DNA of 152 participants were obtained from a previous study in Dr. Kariadi Hospital, Semarang, Indonesia. PCR-RFLP analysis was performed for detection of PNPLA3 rs738409 and ADIPOQ rs2241767 variants. The diagnosis and severity of NAFLD were assessed according to NAFLD activity score (NAS) based on histopathology assessment of liverbiopsy.ResultsAllele G of PNPLA3rs738409 was associated with NAFLD in both bivariate (p=0.009, OR 2.52, CI 95% 1.25–5.07) and multivariate (p=0.008, OR 2.62, CI 95% 1.29%–5.32%) analysis, while ADIPOQ rs2241767 had no significant association. In NAFLD participants, both genotypes showed allele G was higher in the group of possible non-alcoholic steatohepatitis (NASH) – NASH (NAS >2) than in the simple steatosis group (NAS <2) i.e. 40.0% vs. 3.75% for the rs2241767 variant and 23.75% vs. 1.25% for the rs738409 variant, without significant association.ConclusionVariant PNPLA3 rs738409 was associated with NAFLD incidence in studied population. Among NAFLD participants, the frequency of both variants were found higher in the possible NASH – NASH group, yet needs to be confirmed with more participants and a multicenter study.Data and DNA of 152 participants were obtained from a previous study in Dr. Kariadi Hospital, Semarang, Indonesia. PCR-RFLP analysis was performed for detection of PNPLA3 rs738409 and ADIPOQ rs2241767 variants. The diagnosis and severity of NAFLD were assessed according to NAFLD activity score (NAS) based on histopathology assessment of liverbiopsy.
Antioxidant Total and HOMA-IR of Diabetic Rats Given Crocatum piper and Andrographis paniculata Leaf Extracts Nesti Rahmawati; Kusmiyati DK; Diana Nur Afifah
Journal of Biomedicine and Translational Research Vol 7, No 2 (2021): August 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i2.11524

Abstract

Background: Type-2 diabetes mellitus (T2DM) is a silent killer which the prevalence continues to increase in every year. Increased oxidative stress occurs in T2DM. Red betel and bitter herb leaf extracts (RBBH) contain a lot of antioxidants. This combination is expected to provide better safety than if used singly because the content of andrographolid in bitter herb has effect such as nausea, vomiting, loss of appetite, and antifertility if consumed in high doses.Objective: The study aimed to prove the effect of red betel and bitter herb leaf extracts on antioxidant total and Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) in T2DM rats given high-fat diet and Streptozotocin (STZ) induction.Methods: Experimental randomized study with pre-post-test control group design using 25 Sprague Dawley male rats. T2DM model was conducted by providing high-fat feed for 14 days and induction of Streptozotocin-Nicotinamide, then given a combination of red betel and bitter herb leaf extracts at doses of 237.5 mg/kg BW, 225 mg/kg BW, and 212.5 mg/kg BW for 21 days. The measurement of antioxidant total used 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) method. HOMA-IR measured by ELISA (Enzyme-linked immunosorbent assay) method using insulin level equation and fasting blood glucose level measured by glucose oxidase-peroxidase aminoantrypirin (GOD-PAP). Data analysis used paired t-test, wilcoxon test, and ANOVA test to analyze differences in antioxidant total and HOMA-IR value among groups and followed by Bonferroni post-hoc test.Results: All treatments could reduce HOMA-IR and significantly increase antioxidant total (p<0.05). The most decrease in HOMA-IR and increase in antioxidant total at dose 237.5 mg/kg BW of red betel and bitter herb leaf extracts.Conclusion: The combination of red betel and bitter herb leaf extracts with dose 237.5 mg/kg BW, 225 mg/kg BW, and 212.5 mg/kg BW can improve blood glucose, insulin, and HOMA-IR levels in type-2 diabetes mellitus rats. 
Delayed Puberty in Girls with Primary Amenorrhea: A Report of Cases Fatinah Shahab; Inu Mulyantoro; Hary Tjahjanto; Tri Indah Winarni; Sultana MH Faradz
Journal of Biomedicine and Translational Research Vol 7, No 2 (2021): August 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i2.12054

Abstract

Background:Female puberty starts when the pituitary hormone producing follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which will stimulate the ovaries to produce estrogen. Delayed puberty with primary amenorrhea in female is the lack of breast development followed by the absence of menses 3 years after the initiation of breast development. Sex chromosomes have an important role in determining the sex, germ cell differentiation of foetus, and reproductive functions of an offspring, thus, sex chromosomal aberrations frequently cause primary amenorrheaCase presentation: We report two delayed puberty cases with the chief complain of primary amenorrhea. Both cases showed hypoplasia of uterus and ovaries on pelvic imaging and hormonal assay showed low of FSH. The first case was gonadal dysgenesis with 46,XX karyotype and low level of estrogen and the second case was a turner syndrome with 45,X karyotype and normal level of estrogen. Conclusion:This study reported delayed puberty with primary amenorrhea cases due to different chromosomal aberration pattern which have similar clinical features. Therefore, cytogenetic examination is needed for any primary amenorrhea cases in order to accomplish the confirmatory diagnosis and for the clinicians to make a correct intervention and treatment. 
The Effect of Black Garlic (Allium sativum Linn) on Cardiac and Aortic Histopathology in Experimental Studies in Obesity Rats Andreas Arie Setiawan; Fairuz Azmila Purnomo; Vega Karlowee; Noor Wijayahadi
Journal of Biomedicine and Translational Research Vol 7, No 2 (2021): August 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i2.11686

Abstract

ABSTRACTBackground: Obesity is a disorder or disease characterized by the accumulation of excess fat in the body due to an imbalance in energy intake that is used for a long time. Accumulation of fat can reduce adiponectin, causing cardiac hypertrophy, endothelial vasodilation, and other cardiovascular diseases. Black garlic have high antioxidants in the form of S-Allylcysteine(SAC) which functions to increase adiponectin. Objective: To determine the effect of Black garlic on the histopathological picture of the heart and aorta of obese rats. Methods: This study was an experimental study with a randomized post-test only design with control group design with 5 groups of male white rats Sprague Dawley (Rattus novergicus) fed High Fat Fructose. Diet (HFFD) enriched with 1.25% cholesterol and 0.5% cholic acid for 8 weeks and was given black garlic intervention at doses of 450 mg / 200BW, 900mg / 200BW and 1350mg200 / BW for 4 weeks. Results: Giving black garlic significantly reduced body weight of rats (p = 0.001), and the results did not significantly reduce heart weight (p = 0.147), aortic weight (p = 0.061), histopathological changes in heart wall thickness (p = 0.423) and aortic wall thickness (p = 0.802). The effective doses of black garlic in this study were 450 mg / 200 grams BW, 900 mg / 200 grams rat BW and 1350 mg / 200 grams BW of rats. The optimal dose is 900 mg / 200 grams BW. Conclusion: Black garlic gave a significant reduction in body weight of rats and no significant reduction in heart weight, aortic weight, cardiac and aortic histopathological features. 
Effect of Probiotic Supplementation on Sprague Dawley Rat Liver Histopathology Fed by High Fat High Fructose Diet Kusumawati, Ninung Rose Diana; Panunggal, Damianus Galih; Mexitalia, Maria; Sidhartani, Magdalena; Pratiwi, Juwita; Utari, Agustini
Journal of Biomedicine and Translational Research Vol 7, No 2 (2021): August 2021
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i2.11918

Abstract

Background: Non-alcoholic fatty liver disease (NAFLD) is an important cause of liver disease burden worldwide. The gastrointestinal microbiota has a close relationship with the liver as the liver is most exposed to intestinal bacteria. Microbial manipulation is a potential and effective therapy as an alternative in the management of NAFLD/NASH. It has been found that probiotics prevent NAFLD/NASH. However, the study about the protective effect of probiotics on NAFLD/NASH is still limited. Objective: The objective of this study is to evaluate the effect of probiotics on liver histopathology Sprague Dawley rats which given high-fat high fructose (HFHFr) dietMethods: This study is a murine-model post-test-only control study group design. The samples were 21 Sprague Dawley male rats in 7 – 8 weeks of age and were divided into three groups. The Control Group (C) was provided with a standard chow diet for eight weeks. The Non-Probiotic (NP) group was given a High-Fat High Fructose (HFHFr) diet for eight weeks. The Probiotic group (P) was given a HFHFr diet for eight weeks, and a combination of HFHFr and probiotic supplementation consisted of Lactobacillus acidophilus, Bifidobacterium longum, and Streptococcus thermophilus for the next eight weeks. Histopathological samples were obtained from liver biopsy to assess steatosis, NAFLD activity score (NAS), and fibrosis stages. Wilcoxon test was done to analyze body weight before and after treatment. We analyzed the difference in histopathological results using the Mann-Whitney test.Results: We found a significant difference in NAFL and NAS Score between NP and P group. The P group was shown to have lower trends for NAFLD and NASH than the NP group, but not for fibrosis. There is no significant difference between pre and post-test body weight. Conclusion: Probiotics supplementation has a protective effect on liver histopathology against disturbances caused by the HFHFr diet.
The Correlations between Cord Blood Leptin and Leptin Level at Six Months with Infant Growth Ika Rara Rosita; Agustini Utari; Maria Mexitalia
Journal of Biomedicine and Translational Research Vol 7, No 2 (2021): August 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i2.11821

Abstract

Background: Leptin plays an important role in regulating body weight, metabolism, and reproductive functions. Leptin affects metabolism by reducing nutrient intake and increasing energy expenditure which eventually also plays a role in infant growth.Objective: This study aims to determine the relationship between leptin levels and infant growth age 0-6 months.Methods: A prospective cohort study was done for six months on 38 infants, age 0-6 months, from breastfeeding mothers with normal pregnancies. The samples were taken twice, firstly when the infant was born using an umbilical cord blood sample, and secondly at the age of six months, using a vein blood sample. Serum leptin levels were measured using the ELISA method. Infant growth was assessed using WHO 2005’s z-scores.Results: A total of 50 babies were included in the study, 38 of them had been studied completely. Significant correlations were found between the mean of the umbilical cord and six months of age leptin levels (p <0.001), between delta leptin with WHZ and delta leptin with WAZ at six months of age (p = 0.002 and p = 0.003, respectively), and between leptin levels with WHZ (p<0.001) and leptin levels with WAZ (p = 0.004) at six months of age. Leptin levels at the age of six months are lower than umbilical cord blood leptin. Conclusion: The greater decrease of leptin level in the first six months is associated with better infant growth.

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