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INDONESIA
Biomedical Engineering
Published by Universitas Brawijaya
ISSN : -     EISSN : -     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
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Articles 2 Documents
 1 
Search results for , issue "Vol 2, No 2 (2016): Biomedical Engineering" : 2 Documents clear
A Cross Correlation Between Karyotyping and Anthropometric Measurement of Down Syndrome at Malang, Indonesia Antonius Christianto Christianto; Haryudi Aji Cahyono; Djoko Wahono Soemadtji; Fatchiyah Fatchiyah Fatchiyah
Biomedical Engineering Vol 2, No 2 (2016): Biomedical Engineering
Publisher : Biomedical Engineering

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.221 KB)

Abstract

Objective:   Herein, we reported a first study of Down syndrome chromosome analysis in Malang, Indonesia and correlation between phenotype, anthropometric measurement and karyotyping dataMaterial and methods: Karyotyping was conducted using peripheral blood culture.Result:   The karyotyping result showed that from nine patients with DS, we found two types of DS, trisomy 21 in seven patients and mosaicism in two patients. Interestingly, two patients with mosaicism have different percentages of cells with abnormal chromosomes (trisomy 21). In our cases, lower percentages of cells with abnormal chromosomes, the morphologies of patient were more likely normal control.Conclusion:    At least in, at least in part the ratio between lower and upper segment was proved to be different between normal and DS patients.
Detection of Hepatitis C Virus RNA in Blood Donors with Nested PCR Technique Supiana Dian Nurtjahyani; Mochammad Amin Amin; Retno Handajani Handajani
Biomedical Engineering Vol 2, No 2 (2016): Biomedical Engineering
Publisher : Biomedical Engineering

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.443 KB)

Abstract

Objective: This study was conducted to detect hepatitis C virus (HCV) RNA among blood donors in Tuban, East Java, Indonesia,  using nested Polymerase Chain Reaction (PCR) technique.Material and methods: Seven blood donors  with positives anti-HCV were obtained from 500 blood donors from Red Cross Blood Donor Center (Palang Merah Indonesia = PMI) Tuban, East Java, Indonesia, consisting of 375 men (75%) and 125 women (25%). Detection of anti-HCV antibody prevalence in the blood donors was done by using Enzyme-Linked Immunosorbent Assay (ELISA).  The detection of HCV RNA in blood donors  sera  with positives  anti-HCV were performed using nested-PCR technique with three set of primer pairs targeting the NS5B and 5’UTR regions of HCV.  Further amplification products were visualized on a 2% agarose gel containing ethidium bromide under  UV illumination.Result: Seven positives anti-HCV blood donors sera (1.4%) were obtained from 500 blood donors sera, in PMI Tuban, East Java, Indonesia. Out of seven blood donors sera with positives anti-HCV, 6 sera (85,7%)  were positives HCV RNA by using nested PCR technique. Five HCV RNAs were detected with two set of primers based on NS5B HCV region and 1 positive HCV RNA was detected with another set of primer based on 5’UTR HCV region.Conclusion: HCV RNAs were detected in 85,7% positives anti-HCV blood donors using nested PCR technique, in PMI Tuban, East Java. Further research is needed to determine the  genotype  and  subtype  of  HCV. 

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