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Biomedical Engineering
Published by Universitas Brawijaya
ISSN : -     EISSN : -     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Arjuna Subject : -
Articles 2 Documents
 1 
Search results for , issue "Vol 3, No 1 (2017): Vol. 3 No. 1 Biomedical Engineering" : 2 Documents clear
Detection of Gen Chloramphenicol Acetyl Transferase (CAT ) Salmonella typhi Resistant Chloramphenicol by Polymerase Chain Reaction (PCR) Technique Supiana Dian Nurtjahyani; Retno Handajani
Biomedical Engineering Vol 3, No 1 (2017): Vol. 3 No. 1 Biomedical Engineering
Publisher : Biomedical Engineering

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.416 KB)

Abstract

Objective: Salmonella typhi as a causative agent of typhoid abdominalis many militias, which was resistant to the drug, one of which is chloramphenicol. Detection of Salmonella typhi serologically generally done so if there is a problem in the diagnosis difficult to overcome so it needs a faster diagnosis techniques with accurate results. PCR can be used as an alternative solution to these problems. The purpose of this study was to detect the DNA of Salmonella typhi resistant to chloramphenicol by PCR.Material and methods: The method in this population experimental laboratory conducted in the laboratory biology and laboratory Unirow Tuban ITD Airlangga University Surabaya. Experiment done using PCR from blood samples. Using primer from CAT gene to detect this gene.Result: The results of this study with tank PCR can detect DNA of chloramphenicol resistant Salmonella typhi with a length of 293 bp.Conclusion: The conclusion PCR technique can detect DNA of Salmonella typhi resistant to chloramphenicol.
Effectiveness Of Isometric And Range Of Motion (Rom) Exercise Toward Elderly Muscle Strenght In Pasuruan Integrated Service Unit, Elderly Social Services In Lamongan Nurus Safa'ah; Ika Desti Srimurayani
Biomedical Engineering Vol 3, No 1 (2017): Vol. 3 No. 1 Biomedical Engineering
Publisher : Biomedical Engineering

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.858 KB)

Abstract

Objective: Aging is a physiological process that will reduces the function of all organs, among other the musculoskeletal system that cause decreased muscle strenght. Under less of the muscle strenght will reduces activities and mobilization ability. Isometric and Range Of Motion (ROM) exercise is efficient alternative exercise for the elder who will begin the exercise program. This study was aimed to explain the difference of effectiveness between isometric and ROM exercise toward elderly muscle strenghtMaterial and methods: This study used to Pre Test and Post Test Design with time period approach involved 36 respondents, taken by purposive sampling. They were divided in to 2 groups; one group of 18 respondents underwent isometric exercise and the other group of 18 respondents did ROM exercise. Data were collected by checklist. Data were analyzed using Mann Whitney Test with level of significance α ≤ 0,05.Result:  Based on the result of research there were 8 (44,4%) respondents who had increased muscle strength after did isometric exercise, and were 14 (77,8%) respondents who had ones with ROM exercise. The analysis result using Mann Whitney Test showed p = 0,044 it means that H0 is rejected, so there are difference of effectiveness between isometric and Range Of Motion (ROM) exercise toward elderly muscle strenght. Range Of Motion (ROM) exercise was proven more effective to increase elderly muscle strenght who have normal movement, while isometric exercise was recommended to maintain muscle strength for the elderly who get the injured extremity and contraindications to do movement.Conclusion:  It can be used as a program to create healthy elderly and using optimally their time to do better things, such as doing ROM and isometric exercise.

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