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Research Journal of Life Science
Published by Universitas Brawijaya
ISSN : -     EISSN : 23559926     DOI : https://doi.org/10.21776/ub.rjls
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
 1 
Search results for , issue "Vol 10, No 2 (2023)" : 5 Documents clear
Antifungal activity of clove (Eugenia caryophyllata) extract against Fusarium oxysporum cutinase enzyme in Tomato (Solanum lycopersicum) from in vitro study Khairunnisa', Amalia; Syib’li, Muhammad Akhid; Abadi, Abdul Latief
Research Journal of Life Science Vol 10, No 2 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.02.3

Abstract

Fusarium oxysporum f.sp. lycopersici presents a notable threat to tomato plants due to the hydrolytic activity of its cutinase enzyme, which facilitates the penetration of plant root tissues. Thus, reducing cutinase activity through the application of cloves as inhibitors offers an environmentally friendly alternative to hazardous chemical fungicides, showing great promise in controlling Fusarium wilt disease, particularly by targeting the cutinase enzyme of Fusarium oxysporum. This study involved GC-MS analysis of clove extract, fungal growth and cutinase activity assay using rhodamine-b olive oil media. Hence, this study aims to reveal the potential and mechanism of clove extracts as inhibitors of cutinase enzymes to reduce the penetration of the Fusarium oxysporum pathogen in tomato plants. The result shows that clove extract, containing approximately 20% antifungal compounds primarily eugenol (11% of total peak area), inhibits Fusarium oxysporum growth. It also reduces the hydrolytic activity of cutinase crucial for fungal penetration, evidenced by decreased fluorescence halos in tests with rhodamine-B and olive oil media under UV light after adding 1%, 2%, and 3% clove extract. Further in vivo studies are needed to explore its direct effects as a plant inhibitor.
In Vitro Exploration of Endophytes Bacteria from Platycerium Elephantotis Plants as Nitrogen Fixers and Phosphate Solubilizers Pamungkas, Bayu Aji; Aini, Luqman Qurata; Abadi, Abdul Latief; Trianti, Irisa; Syib`li, Muhammad Akhid; Setiawan, Adi
Research Journal of Life Science Vol 10, No 2 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.02.4

Abstract

Platycerium elephantotis, an African epiphytic fern thriving in Indonesia, is prized for its ornamental value and medicinal potential. It aids in fern succession without harming its host, obtaining nutrients from leaf litter and roots. Special water-storing tissues and CAM photosynthesis enhance its resilience. Endophytic bacteria in its roots likely contribute to nutrient absorption, including phosphate solubilization and nitrogen fixation, similar to other fern species. Studies on these bacteria are crucial for enhancing agricultural practices by reducing chemical inputs and promoting plant growth as plant growth-promoting bacteria (PGPB), particularly in nitrogen fixation and phosphate solubilization. The research was conducted from November 2023 to January 2024. Plant samples were collected from Arjuna Park Conservation Garden, Malang, and endophytic bacteria were isolated using serial dilution and nutrient agar media. Hypersensitivity, phosphate solubilization, and nitrogen fixation tests were conducted to assess bacterial traits. The study obtained six bacterial isolates (PE 5, PE 7, PE 24, PE 25, PE 30, and PE 49) from P. elephantotis with potential as biofertilizers due to their nitrogen-fixing and phosphate-solubilizing abilities. The diverse colony morphologies indicate different bacterial species. All isolates are rod-shaped (bacilli). Gram staining shows that PE 25 and PE 49 are gram-negative, while PE 5, PE 7, PE 24, and PE 30 are gram-positive.
Development of Cadra cautella Walker (Lepidoptera: Pyralidae) on Peanuts, Almonds, and Cashews Rahayu, Fitria Ariani; Astuti, Ludji Pantja; Rizali, Akhmad
Research Journal of Life Science Vol 10, No 2 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.02.5

Abstract

Cadra cautella Walker pest (Lepidoptera: Pyralidae) is a group of significant pests that can attack various stored products. The presence of C. cautella can cause damage to stored products, i.e. holes, contamination, and weight loss of stored products. This study aimed to determine the development time and life cycle of C. cautella in peanuts, almonds, and cashews. This research consisted of three diets repeated ten times and arranged in a Completely Randomized Design. This research carried out by preparing 30 eggs (0–24 h old) in each insect diet. The observed variables consist of the development of each phase (eggs, I-V instar larvae, prepupae pupae, adult), life cycle, longevity, fecundity, and fertility. The result showed that the diet type significantly affected the development of C. cautella. The developmental time and life cycle of C. cautella on cashews was shorter than on peanuts and almonds. Furthermore, the fecundity and fertility of C. cautella on cashews was higher than on peanuts and almonds. The results showed that cashews were of the suitable diets for the development of C. cautella.
Resistance of two sugarcane genotypes towards Sporisorium scitamineum Scoring Using Novel Method on Sugarcane buds tissue and Phenol Plant dynamics Supriyono, Supriyono; Abadi, Abdul Latief; Syib’li, Muhammad Akhid; Hidayah, Nurul
Research Journal of Life Science Vol 10, No 2 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.02.1

Abstract

Smut disease caused by a basidiomycete fungus, Sporisorium scitamineum, is one of major diseases on sugarcane worldwide. The disease could cause considerable yield losses up to 60% on susceptible varieties. Understanding of resistance characteristics of sugarcane clones is essential to support development of resistant sugarcane varieties to smut fungus infection. This study aimed to determine characteristics of sugarcane genotypes J19R17-325/15/3-9 and ASA Agribun toward S. scitamineum infection and the role of phenolic compound on defense mechanism of sugarcane to smut infection. The research consisted of four treatments and was arranged according to the randomized block design with five replicates. The results revealed that J19R17-325/15/3-9 produced higher phenolic compound (0,192 mg/gr) rather than ASA Agribun (0,182 mg/gr). In addition, the distribution of fungal mycelia within sugarcane tissues on J19R17-325/15/3-9 was also lower than those on ASA Agribun. These results indicated that J19R17-325/15/3-9 might have resistance properties to S. scitamineum infection.
Effect of Weather Conditions to Vascular Streak Dieback Disease Severity on Some Cocoa Clones with Different Resistant Levels Aini, Febrilia Nur; Aini, Luqman Qurata; Syibli, Muhammad Akhi; Sari, Indah Anita; Susilo, Agung Wahyu; Setyawan, Bayu; Malik, Abdul; Budiman, Aris
Research Journal of Life Science Vol 10, No 2 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.02.2

Abstract

Vascular streak dieback is a major disease in cocoa trees that affects at in various stages, and effect in significant yield losses. This study aimed to identify the area under the disease progress curve (AUDPC), infection rate (r), and the influence of weather factors on the development of vascular streak dieback disease caused by Ceratobasidium theobromae in some cocoa clones with different resistant levels. The research used a randomized complete block design consisted six clones as treatment and each treatment consisted of three replications. Disease severity was observed on six different cocoa clones and observations were conducted monthly. The observation of disease severity was observed monthly for 12 months and then used to analyze AUDPC and infection rate. Monthly data on the weather conditions were taken from the climatology station of the Indonesian Coffee and Cocoa Research Center, East Java. The results showed that Sulawesi 01 and ICCRI 09, categorized as resistant clones, showed low AUDPC values, 0.02 and 2.85 respectively. The cocoa clones Sulawesi 02 and MCC 02, categorized as tolerant clones, had higher AUDPC values, 19.87 and 34.27 respectively. The cocoa clones BCL and BL 50 showed the highest AUDPC values, 277.05 and 265.70 respectively. Path analysis on resistant clones indicated that rainfall is the most influential weather factor in increasing the severity of vascular streak dieback, followed by temperature. Higher relative humidity can significantly reduce disease severity, while sunlight and wind speed have smaller effects. In contrast, path analysis on susceptible clones showed that temperature, rainfall, relative humidity, and wind speed all positively influence the severity of vascular streak dieback. At the same time, sunlight has a mitigating effect.

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