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All Journal Research Journal of Life Science Plantropica: Journal of Agricultural Science AGRIVITA, Journal of Agricultural Science Agro Bali: Agricultural Journal Jurnal HPT (Hama Penyakit Tumbuhan)
Irisa Trianti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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In Vitro Exploration of Endophytes Bacteria from Platycerium Elephantotis Plants as Nitrogen Fixers and Phosphate Solubilizers Pamungkas, Bayu Aji; Aini, Luqman Qurata; Abadi, Abdul Latief; Trianti, Irisa; Syib`li, Muhammad Akhid; Setiawan, Adi
Research Journal of Life Science Vol 10, No 2 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.02.4

Abstract

Platycerium elephantotis, an African epiphytic fern thriving in Indonesia, is prized for its ornamental value and medicinal potential. It aids in fern succession without harming its host, obtaining nutrients from leaf litter and roots. Special water-storing tissues and CAM photosynthesis enhance its resilience. Endophytic bacteria in its roots likely contribute to nutrient absorption, including phosphate solubilization and nitrogen fixation, similar to other fern species. Studies on these bacteria are crucial for enhancing agricultural practices by reducing chemical inputs and promoting plant growth as plant growth-promoting bacteria (PGPB), particularly in nitrogen fixation and phosphate solubilization. The research was conducted from November 2023 to January 2024. Plant samples were collected from Arjuna Park Conservation Garden, Malang, and endophytic bacteria were isolated using serial dilution and nutrient agar media. Hypersensitivity, phosphate solubilization, and nitrogen fixation tests were conducted to assess bacterial traits. The study obtained six bacterial isolates (PE 5, PE 7, PE 24, PE 25, PE 30, and PE 49) from P. elephantotis with potential as biofertilizers due to their nitrogen-fixing and phosphate-solubilizing abilities. The diverse colony morphologies indicate different bacterial species. All isolates are rod-shaped (bacilli). Gram staining shows that PE 25 and PE 49 are gram-negative, while PE 5, PE 7, PE 24, and PE 30 are gram-positive.
Exploration of Pathogenic Microbes and Antagonistic Agents in Apple Production Centers in Malang Regency Abadi, Abdul Latief; Choliq, Fery Abdul; Trianti, Irisa; Maknunin, Lu'lu'il; Yulianti, Novi Dwi
Plantropica: Journal of Agricultural Science Vol. 9 No. 1 (2024)
Publisher : Department of Agronomy, Faculty of Agriculture, Brawijaya University

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Abstract

The apple farming group is one of the built of Department of Plant Pests and Diseases, Agricultural Faculty, Brawijaya University. In 2019, this departmen received a request from apple farmers in Madiredo Village, Pujon District to held an assistance programs of apple cultivation and environmentally pest control. This is related with the increasing of fruit rot and other diseases which is caused a decrease of apple production especially in Malang Regency. In general, apple farmers still rely on disease control using chemical fungicides which can have a negative impact on the environment and create resistance to pathogenic fungi. Therefore, this research was carried out to explore and study the potential of biological agents in apple production centers in Malang Regency, specifically in Poncokusumo, Pujon, and Batu District on conventional fields and mixed fields. This research was held observationally through fields surveys and farmer interviews, took soil samples and disease samples then then isolating, identifying and testing for antagonism at laboratory. Based on interviews and observation of symptomology, two dominant diseases were found, namely fruit rot disease (Gloeosporium sp.) and marsonina leaf spot disease (Marssonina coronaria). The results of exploration of the rhizospheric apple plants obtained isolates on conventional fields in Kec. Pujon has the highest abundance of fungal colonies compared to other regions. Based on the results of the antagonism test for pathogenic fungi and biological agents, it was found that the isolate codes KH.C, KK.A, JK.B, JK.J, and BK.B had the highest percentage of inhibition or the most effective in controlling the pathogen Gloeosporium sp. So in the next, the species can be identified molecularly.
Efficacy of Biofungicide with Active Ingredients Trichoderma sp. Against Late Blight Disease (Phytophthora infestans) in Potato Plants Abadi, Abdul Latief; Trianti, Irisa; Choliq, Fery Abdul; Sektiono, Antok Wahyu; Yulianti, Novi Dwi
Agro Bali : Agricultural Journal Vol 7, No 1 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i1.1591

Abstract

The cultivation of potatoes in Indonesia faces challenges, particularly a decline in production attributed to the devastating impact of late blight caused by the fungus P. infestans. Traditional control methods involve the frequent use of chemical pesticides, which pose environmental and health risks. To address this, the research aimed to assess the efficacy of biofungicide containing Trichoderma sp. as a biological agent in controlling late blight on potatoes. The study, conducted in Sumberbrantas Village, Bumiaji District, Batu City, East Java, and the plant disease laboratory at Brawijaya University from January to April 2023, employed both in vivo and in vitro experiments. A Randomized Block Design (RBD) was used for in vivo research, while a Completely Randomized Design (CRD) was employed for in vitro studies, each comprising five concentration treatments with five repetitions. Parameters such as disease intensity, efficacy level, potato production, and biofungicide inhibition were observed. Analysis of variance and the Tukey Honestly Significant Difference (HSD) test were applied to the data and processed using Microsoft Excel. The results indicated that biofungicide with Trichoderma sp. concentrations of 6 ml/l, 4.5 ml/l, 3 ml/l, and 1.5 ml/l effectively reduced the intensity of late blight on potatoes, with efficacy levels exceeding 50%. The highest potato production occurred with a concentration of 6 ml/l, yielding 15.44 tons/ha, followed by 14.67 tons/ha for the 4.5 ml/l concentration. The biofungicide with a concentration of 6 ml/l exhibited the lowest disease intensity at 14.28% and the highest efficacy at 83.08%. Inhibition tests demonstrated that concentrations of 6 ml/l and 4.5 ml were highly effective, inhibiting P. infestans growth by an average of 62.04% and 59.90%, respectively. These findings highlight the potential of biofungicide with Trichoderma sp. in managing late blight on potatoes, providing a sustainable and environmentally friendly alternative to chemical pesticides.
INDUKSI KETAHANAN SISTEMIK PADA TANAMAN BAWANG MERAH (Allium ascalonicum) OLEH BAKTERI ENDOFIT YANG DIISOLASI DARI Platycerium elephantotis: INDUCED SYSTEMIC RESISTANCE IN SHALLOT (Allium ascalonicum) BY ENDOPHYTIC BACTERIA ISOLATED FROM Platycerium elephantotis Pamungkas, Bayu Aji; Aini, Luqman Qurata; Abadi, Abdul Latief; Syib`'li, Muhammad Akhid; Setiawan, Adi; Trianti, Irisa
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 13 No. 4 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2025.013.4.5

Abstract

Produksi bawang merah dihadapkan pada kendala serangan organisme pengganggu tanaman (OPT). Pemberian bakteri endofit diduga dapat menginduksi ketahanan tanaman bawang merah. Penelitian ini bertujuan meningkatkan ketahanan dan produktivitas tanaman bawang merah melalui aplikasi bakteri endofit asal Platycerium elephantotis yang berpotensi sebagai Plant Growth Promoting Bacteria (PGPB). Penelitian ini dilakukan di Desa Dadaprejo, Kota Batu, dengan menggunakan varietas bawang merah Tajuk, yang ditanam menggunakan rancangan acak kelompok dengan tujuh perlakuan isolat bakteri endofit dari P. elephantotis (PE 5, PE 7, PE 24, PE 25, PE 30, dan PE 49) serta satu kontrol. Pengamatan serangan OPT dilakukan setiap minggu, sementara analisis aktivitas enzim pertahanan tanaman dilakukan pada umur 55 hari setelah tanam. Hasil penelitian menunjukkan bahwa isolat bakteri Bacillus cereus PE 5 mampu menurunkan kejadian penyakit moler (Fusarium oxysporum f.sp. cepae) secara signifikan pada tanaman bawang merah dibandingkan dengan kontrol, sehingga membuktikan kemampuannya dalam meningkatkan ketahanan tanaman. Isolat PE 5, PE 30, dan A. nosocomonalis PE 49 juga meningkatkan aktivitas enzim peroksidase yang berperan dalam penguatan dinding sel dan sintesis senyawa antimikroba, sehingga memperkuat respons pertahanan tanaman. Meskipun demikian, aplikasi isolat bakteri tidak berpengaruh nyata terhadap produksi fenol, yang diduga dipengaruhi oleh serangan hama S. exigua yang merata pada seluruh tanaman. Temuan ini mengindikasikan bahwa aplikasi PGPB, terutama isolat Bacillus cereus  PE 5, berpotensi meningkatkan ketahanan tanaman bawang merah.
In Vitro Assessment of Endophytic Bacteria Originating from Cycas rumphii and Dioon spinulosum as Stress-Tolerant Plant Growth-Promoting Bacteria Al Husni, Muhammad Fariq; Trianti, Irisa; Yusnawan, Eriyanto; Pamungkas, Bayu Aji; Putri, Sastia Prama; Aini, Luqman Qurata
AGRIVITA Journal of Agricultural Science Vol 48, No 1 (2026): IN PRESS
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v48i1.5072

Abstract

The ability of cycads to grow in harsh environments is thought to be associated with endophytic bacteria in their roots. This study aimed to characterize endophytic bacteria from Cycas rumphii and Dioon spinulosum as stress-tolerant plant growth-promoting bacteria (PGPB). Fine roots of C. rumphii and D. spinulosum were isolated using serial dilution plating and characterized through colony morphological observation, hypersensitivity, antagonistic activity, PGP activity, and abiotic stress tolerance assays. Fourteen isolates were obtained (eight from C. rumphii and six from D. spinulosum), with higher abundance and diversity in D. spinulosum (2.51 × 10¹⁰ CFU/g; H′ = 2.016) than C. rumphii (4.64 × 10⁹ CFU/g; H′ = 1.752). All isolates were non-pathogenic; 92% fixed nitrogen, 92% solubilized phosphate, and one (DS1A) produced IAA (0.200 ppm). Four isolates showed antagonism against Xanthomonas campestris (0.40–0.67 cm inhibition) and tolerance to drought, salinity (5–10%), and moderate heat, with growth at pH 5–6. Molecular identification by 16S rRNA sequencing revealed that CR1A, CR1B, and DS1B belong to Stenotrophomonas maltophilia, whereas DS1A belongs to Bacillus sp. These findings highlight cycads as a source of endophytic PGPB candidates for supporting plant health, particularly when grown in the environmental stress condition areas.
Co-Authors Abdul Latief Abadi Achmad Noerkhaerin Putra Adi Setiawan Al Husni, Muhammad Fariq Eriyanto Yusnawan Fery Abdul Choliq Luqman Qurata Aini Maknunin, Lu'lu'il Pamungkas, Bayu Aji Putri, Sastia Prama Sektiono, Antok Wahyu Syib`'li, Muhammad Akhid Syib`li, Muhammad Akhid Yulianti, Novi Dwi