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Research Journal of Life Science
Published by Universitas Brawijaya
ISSN : -     EISSN : 23559926     DOI : https://doi.org/10.21776/ub.rjls
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
 1 
Search results for , issue "Vol 9, No 3 (2022)" : 5 Documents clear
Identification of Aflatoxigenic Fungi in Myristica fragrans using V8 and CAM Media Arifah, Fitri; Aini, Luqman Qurata; Muhibuddin, Anton; Dianti, Sylvia; Prabowo, Matheus Randy
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.3

Abstract

Nutmeg is a spice that might be susceptible to being infected with aflatoxigenic fungi. Aflatoxins are mycotoxins synthesized by certain strains of Aspergillus section Flavi. However, not all strains are able to produce aflatoxins. The approach frequently employed for this survey comprises the cultivation of strains in a suitable liquid or solid medium and their subsequent extraction and analysis for the presence of aflatoxins. To date, very few studies have been conducted on the identification of contaminate and aflatoxigenic fungi in Myristica fragrans. Therefore, this study aimed to identify the contaminate and aflatoxigenic fungi in Myristica fragrans growing on V8 and CAM Media. Isolation of the fungus was carried out by direct agar plating. Lasiodiplodia theobromae, Aspergillus niger, A. tamarii, Penicillium citrinum, Rhizopus delemar, A. nomiae, and A. aflatoxiformans were recovered from nutmeg kernels sampled from North Minahasa, North Sulawesi, Indonesia. The findings of fungal isolation demonstrated that L. theobromae was the most prevalent form of fungus detected in the nutmeg kernels (50%), followed by A. niger (38.13%) and A. tamarii (10%). A. nomiae and A. aflatoxiformans were the aflatoxigenic fungi isolated from M. fragrans kernels.
Association between Predatory Arthropods and Weeds on Sugarcane Plants Devi, Mia Prastika; Rahardjo, Bambang Tri; Tarno, Hagus
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.4

Abstract

While a large number of farmers in the tropics consider weeds to be just a nuisance, some weeds can potentially have benefits for cultivated crops. Weeds around cultivated plants can act as a biocide, soil improvement, and a food source for humans and animals, as well as a habitat for some insects. Based on the regression analysist, weed density has a relationship to the diversity of predatory arthropod species (R2= 0.026; P<0.001) and the abundance of individual predatory arthropods (R2= 0.010; P<0.001). Beneficial weeds around sugarcane plantations create mutually beneficial interactions. Weeds can be used as a place to live, reproduce, and produce nutrients for predatory arthropods. Research on the role of weeds against natural enemies from predators in sugarcane agroecosystems is also rarely studied. In this study, the focus was on the association of weeds with the diversity and abundance of predatory arthropods in the sugarcane agroecosystem.
Analysis of Chitinase Enzyme Trichoderma sp. in Degrading Fusarium oxysporum Rosyida, Risya; Martosudiro, Mintarto; Muhibuddin, Anton
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.5

Abstract

The chitinase enzyme in Trichoderma sp. play an important role in pathogen control. This is because chitin is the main component of the fungal cell wall which the chitinase enzyme can degrade. One Trichoderma sp. isolate was obtained (UBPK6) with the highest percentage of inhibition against Fusarium oxysporum, 76.71%. In addition, UBPK6 isolates also showed the highest bromocresol purple reagent test results (indicating the presence of chitinase enzymes). Chitinase enzyme activity was measured for Trichoderma asperellum isolates. The results showed that the optimum incubation period for enzyme production was the 4th day, with an enzyme activity value of 4.05 U/mL. It indicates that this time is the right time for harvesting enzymes. Furthermore, the effect of pH on the chitinase enzyme activity of Trichoderma asperellum fungus. The test results showed that the optimum value was produced at pH 5 with a value of 3.4 U/mL and decreased afterward. The pH five treatment was the best in inhibiting the growth of pathogens, with an inhibition value of 60.63%. The higher the content of the chitinase enzyme, the higher its ability to degrade damage to the germination of pathogenic spores, thus causing the growth of the pathogen to be inhibited.
Yield and Yield Components of Red Onion (Allium ascalonicum L.) Influenced by Application of Amino Acid Fertilizer And Triacontanol Putra, Anggara Ista; Waluyo, Budi; Nihayati, Ellis
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.1

Abstract

Red onions are one of the agricultural products with a relatively high demand for the consumer market in Indonesia, but the productivity figure is quite low when compared to other countries (FAO, 2021). This study seeks to explore the effectiveness of the application of amino acid fertilizer and triacontanol in increasing the yield and yield components of red onions. The study took place in Karangploso District, Malang from April to August 2021. Employing a randomized block design, this study used 2 factors, which are amino acid fertilizers (A1: 1000 ppm; A2: 3000 ppm; A3: 5000 ppm) and triacontanol (T0: 0 ppm; T1: 5ppm; T2: 10 ppm). Each combination is repeated 3 times. As a results, amino acid fertilizers can significantly affect the number of red onions bulbs, yields per hectare, economic weight, and economic weight loss. A significant result was seen in the application of triacontanol on red onions yields per hectare and economic weight. The interaction of characters of bulb length was shown in the combination of 5-10 ppm triacontanol with amino acid fertilizer. The best response, which ranged from 10.64% to 11.67%, was noted in fertilizer with 5000 ppm of an amino acid fertilizer. As the concentration of amino acid fertilizer in combination with triacontanol increased, the total bulb fresh weight increased as well, in which, 5000 ppm of amino acid fertilizer could increase the weight by 45,23-45,43 g clump-1, which was greater than the response of the 1000 ppm amino acid fertilizer combination.   
Fourier Transform Infra-Red (FTIR) Spectrum Characterization of Bacillus mycoides Trisnawati, Luh Putu Adi; Aini, Luqman Qurata; Abadi, Abdul Latief; Prillianti, Kestrillia Rega; Prabowo, Matheus Randy
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.2

Abstract

The presence of Bacillus mycoides and its ability to grow and spread quickly certainly affect the growth of the target pathogen and it can cause invalid detection results. Therefore, the presence of contaminant bacteria needs to be detected to ensure the specificity of the detection results against the target pathogenic bacteria. Various kinds of detection methods are commonly used, such as ELISA (enzyme-linked immunosorbent assay) and PCR (polymerase chain reaction) are time-consuming and not always very specific. Fourier-transform infrared (FTIR) spectroscopy methods were adopted to provide a comprehensive and reliable method for bacterial analysis. In this study, FTIR spectroscopy was used as an initial guess for the identification of bacterial isolates. Our results showed that there are dominant peaks from the FTIR spectrum obtained that were most associated with protein and carbohydrate in the range of wave number 400-4000 cm-1.  

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