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Journal of Tropical Plant Protection
Published by Universitas Brawijaya
ISSN : -     EISSN : 23557974     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
We cordially invite you to submit a manuscript to Journal of Tropical Plant Protection (JTPP), an upcoming regular peer-reviewed open access on-line scientific journal that covers fundamental and applied aspects of plant pathology and entomology in agriculture and natural resources. The journal will consider submissions from all over the world, on research works not being published or submitted for publication as full paper, review article and research note elsewhere.
Arjuna Subject : -
Articles 72 Documents
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Efficacy and Compatibility Test of Pseudomonas fluorescens with Fungicides Pyraclostrobin and Fluxapyroxad Against Alternaria solani in Tomato Plants Hardiana, Sindy Sukma; Abadi, Abdul Latief
Journal of Tropical Plant Protection Vol. 6 No. 2 (2025)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2025.006.2.5

Abstract

Early Blight disease is an important disease of tomato plants caused by the fungus Alternaria solani. This disease attack can cause a decrease in production yields up to 78%. Control efforts using synthetic fungicides continuously can cause new problems for the environment. This research was conducted to determine the effect of fungicide applications with active ingredients Pyraclostrobin+Fluxapyroxad and the addition of biological agent Pseudomonas fluorescens on early blight disease and growth of tomato plants. This study consisted of in vivo and in vitro studies with observation parameters in the form of compatibility of bacteria with fungicides, fungicide inhibition, disease intensity, level of efficacy, and growth of tomato plants. The results showed that the combination of synthetic fungicide and Pseudomonas fluorescens was classified as compatible and effective in controlling Alternaria solani and able to support the growth of tomato plants. Combination treatment can reduce the use of synthetic fungicide by 25% of the recommended dose and is able to give results that are not significantly different from the recommended dose of synthetic fungicide treatment.
Detection of Onion Yellow Dwarf Virus (OYDV) in Onion Commodities Using DAS-ELISA and RT-PCR at The Point of Entry for Carrier Media at Semayang Port, Balikpapan Zulverdi, Yudhika Tsabitah Ramacendani; Martosudiro, Mintarto
Journal of Tropical Plant Protection Vol. 7 No. 1 (2026)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2026.007.1.1

Abstract

Plants of the genus Allium, such as shallots (Allium cepa var. aggregatum), garlic (Allium sativum L.), green onions (Allium fistulosum L.), and onions (Allium cepa var. cepa), are high-value commodities widely consumed in Indonesia. However, Allium production in East Kalimantan does not meet local demand, necessitating supplies from other regions. This inter-regional transport poses a risk of introducing organisms harmful to plants. Among these pests, viruses are particularly challenging to control. According to the Regulation of the Minister of Agriculture No. 25 of 2020, Onion Yellow Dwarf Virus is classified as a quarantine pest under category A2 requiring strict monitoring in Indonesia. Its distribution is currently limited to Java, Bali, West Nusa Tenggara, and Sulawesi. Therefore, accurate detection techniques are critical to prevent the spread of this pathogen to areas still free from Onion Yellow Dwarf Virus. Research results indicate that among the four types of onions, Onion Yellow Dwarf Virus (OYDV) exhibits symptoms in garlic. This finding was also confirmed through DAS-ELISA and RT-PCR testing. The detection results using DAS-ELISA on garlic plants showed an ELISA Reader absorbance value (0.6848) greater than twice the absorbance value of the negative control (0.2982). RT-PCR detection also yielded positive results with the formation of a 500 bp band.
Effect of Landscape Composition on Diversity and Abundance of Hymenoptera Parasitoids in Paddy Crops in Malang Regency, East Java Aprilia, Setiya Dwi; Rizali, Akhmad
Journal of Tropical Plant Protection Vol. 7 No. 1 (2026)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2026.007.1.3

Abstract

The existence of Hymenoptera parasitoids can be influenced by the landscape structure of agricultural land. Optimization of pest control using natural enemies can be done on a landscape scale. This study aimed to examine the characteristics of the rice cultivation landscape at each research location, examine the diversity of Hymenoptera parasitoids and their role in rice cultivation. In addition, this study aims to examine the factors that influence the diversity and abundance of Hymenoptera parasitoids, as well as examine the effect of landscape composition on the diversity and abundance of Hymenoptera parasitoids in rice cultivation in Malang Regency, East Java. The research was conducted at various designated rice planting locations. The research locations were in 4 sub-districts, namely Karangploso, Lawang, Kepanjen, and Tumpang sub-districts with three sampling points in each of these sub-districts. The research began with determining the location or ground survey. Insects were sampled using yellow pan traps,  with two traps installed in each subplot. Samples preserved in vials containing 70% alcohol were then sorted into microtubes according to sampling order, and specimens were examined morphologically to determine morphospecies. Supporting data were obtained from interviews with farmers or land owners. All data were entered into a database in Microsoft Excel and spreadsheets and analyzed using RStudio. The results indicated that the 12 study sites varied widely and were classified as complex landscapes, comprising eight land-use types dominated by natural habitats, paddy fields, and settlements. Hymenoptera parasitoids were found consisting of 8 families and 21 morphospecies. The parasitoids found act as parasitoids of eggs, larvae, eggs-larvae, and larvae-pupae. Altitude and age factors correlated with the diversity of Hymenoptera parasitoids. Landscape composition metrics, including Number of Patches (NP) and Class Area (CA) of rice fields, were correlated with the diversity and abundance of individual parasitoids.
The Potential of Cabbage (Brassica oleracea var. capitata L.) Rhizosphere Bacteria as Bioremediator of Paraquat Dichloride-Contaminated Soil Nawangsih, Handani; Aini, Luqman Qurata
Journal of Tropical Plant Protection Vol. 7 No. 1 (2026)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2026.007.1.2

Abstract

Weeds are plants that inhibit crop growth, development, and productivity. This leads farmers to use pesticides to control them. Herbicides, such as paraquat dichloride, are commonly used by farmers to control weeds. The U.S. Environmental Protection Agency (EPA) reported that 14 out of 41 commonly used pesticides in horticultural commodities are classified as carcinogenic compounds, and pesticide residues have been reported to contaminate 83% of the observed horticultural crops. Therefore, there is a need for technologies to address the issue of pesticide residues, such as bioremediation. The objective of this study was to determine the potential of bacteria in the cabbage rhizosphere, which is rich in diversity, as a bioremediator for maize fields contaminated with paraquat dichloride. The study was conducted at the Department of Plant Pest and Disease Laboratory, Brawijaya University, and farmers' cabbage fields from December 2019 to December 2020. The experimental design was a randomized block design (RBD) with 3 replications and 10 treatments, including a control group without bacteria or herbicide. The results showed that there were eight isolates showed bioremediation potential, and one isolate showed the best performance.
The Influence of Cultivation Techniques on Horticultural Cultivation on The Diversity, Abundance, and Composition of Spiders Salsabila, Shevira Satria; Rahardjo, Bambang Tri
Journal of Tropical Plant Protection Vol. 7 No. 1 (2026)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2026.007.1.5

Abstract

Organic farming is a cultivation technique that applies natural materials and does not use chemical fertilizers and pesticides in cultivation techniques. Differences in cultivation techniques can affect the diversity, abundance, as well as the composition of spiders. This study was conducted to determine the diversity, abundance, as well as composition of spiders can be influenced by different cultivation techniques. The research was conducted from September 2023 to October 2023. Observations were made on organic land and conventional land. Data collection of cultivation techniques is carried out by interviews. Population and diversity data collection is carried out every 5 days within 4 weeks. Specimen collection is carried out by passive and active methods and then identification is carried out. Tabulate data in Microsoft Excel, then analyze it using Rstudio. In the Rstudio analysis, the Shapiro-Wilk test was carried out to determine the normality of the data, further Honest Significant Difference test at the level of 5%. Data analysis carried out in the form of variety analysis or and ANOSIM using the Bray-Curtis test. The results of the study, the number of spiders on organic land was more, namely 65 individuals consisting of 5 orders and 9 morphospecies. The results showed that differences in cultivation techniques had an effect on the diversity and abundance of spiders. The composition of the land shows that there is a significant difference but there are intersecting parts that indicate a similarity in the composition of spiders. The results of data analysis show that organic land has more diversity, abundance, and spider composition than conventional land.
Effect of Plant Growth-Promoting Rhizobacteria (PGPR) Application on Helminthosporium turcicum Infection and the Growth of Sweet Corn (Zea mays saccharata Surt.) Putri, Rosy Cahyagung Dewi Basuki; Abadi, Abdul Latief
Journal of Tropical Plant Protection Vol. 7 No. 1 (2026)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2026.007.1.4

Abstract

The study aimed to determine the effect of application Plant Growth-Promoting Rhizobacteria (PGPR). The research was carried out in the greenhouse dan laboratory of plant disease at the Brawijaya University Malang. The study was arranged in a Randomized Block Design which consisted of 8 treatments and 3 replications. The results showed that the single isolate treatment have the highest average incubation period of Helminthosporium turcicum, namely the single isolate of Bacillus subtilis and Pseudomonas fluorescens bacteria for 9 day. The treatment of the consortium of 3 bacteria, namely Bacillus subtilis+Pseudomonas fluorescens +UB19 (Pseudomonas sp). showed an excellent antagonistic activity in suppressing diseases caused by the fungus Helminthospororium turcicum. Pseudomonas sp. had the ability to increase the plants fresh weight but had no effect on the roots fresh weight.  However, the bacterial consortium (B. subtilis, P. fluorescens, and UB 19 (Pseudomonas sp.)) in sweet corn plant growth was indicated by the higher average yield of plant height, number and weight of roots compared to other treatments.
Evaluation of Indigenous Fruit Juices and Trap Colours for Managing Fruit Flies (Diptera: Tephritidae) in Butternut (Cucurbita moschata) Sibanda, Mzobanzi; Musabayana, Zivanayi; Mathema, Ndabanye; Tembo, Lenon; Zanamwe, Pesanai; Mafirakureva, Tariro; Zhou, Mandhlenkosi; Moyo, Dean; Manjeru, Pepukai
Journal of Tropical Plant Protection Vol. 7 No. 2 (2026)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2026.007.2.1

Abstract

Fruit fly is serious pest that reduce yield and fruit quality in Cucurbita moschata. Cucurbita moschata is an important food and cash crop. Development of eco-friendly strategies to reduce the effects of fruit fly damage is therefore important in ensuring global food security. The present study was carried out to evaluate the potential of combinations of trap colour and indigenous fruit juices, i.e., Sclerocarya birrea and Strychnos spinosa, to attract fruit flies to the traps. A field experiment was laid out as a 4 x 3 factorial design with three replicates of each of the 12 treatments arranged in a Randomized Complete Block Design. The first factor was the type of attractant and it consisted of four levels, i.e., Strychnos spinosa juice, Sclerocarya birrea juice, sugar and water. The second factor was trap colour and it consisted of three levels, viz., red, yellow and green. The number of fruit flies trapped was counted and classified into different fruit fly genera. The results showed that the number of fruit flies attracted by a combination of sugar and the three trap colours was statistically (P < 0.05) similar to number of fruit flies attracted by a combination of Strychnos spinosa juice and the three trap colours and a combination of Sclerocarya birrea juice and the three trap colours. Bactrocera spp has the highest number of flies trapped, with a grand mean of 39.9, followed by Ceratitis spp (15.5) and Drosophila melanogaster had the least number of flies trapped (9.94). It was concluded that Strychnos spinosa and Sclerocarya birrea juices can be used as alternative attractants to sugar for baiting and trapping fruit flies with traps painted yellow, green and red.
Isolation and Morphological Characterization of Colletotrichum spp. Associated with Mango Anthracnose in Mutoko District, Zimbabwe Chabikwa , Nyarai; Chinheya, Cleopas C.; Mapope , Nyamande; Zivanai, Musabayana; Tembo, Lenon
Journal of Tropical Plant Protection Vol. 7 No. 2 (2026)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2026.007.2.2

Abstract

Anthracnose is a fungal disease that severely affects mango (Mangifera indica L.) production globally. In Zimbabwe, mango production is a source of household nutrition and income from informal markets but there has been limited information on the fungal pathogens related to anthracnose in the main production areas. Lack of local baseline data limits proper diagnosis and the development of disease control measures. The main objective of this study was to morphologically characterize fungal isolates that were associated with anthracnose symptoms on mango fruits and leaves, which were collected from different orchards in the Mutoko District of Zimbabwe. Symptomatic samples from various trees were randomly collected in the different orchards. Pieces of tissue were taken from the edge of the lesions and surfaces sterilized, and were inoculation on potato dextrose agar. Colonies of the fungi that appeared were re-cultured to obtain pure culture. The isolates exhibited different colony morphology but mostly they produced grey to dark colonies of cottony to velvety textures. Based on morphology, the isolates were considered members of the Colletotrichum genus; nevertheless, species identification by molecular means was not performed. Through this research, the morphological characteristics of the anthracnose, associated Colletotrichum isolates from the Mutoko District were initially documented. This evidence calls for molecular and pathogenicity analyses to identify the species so that disease management can be effective.
Assessment of Farmers’ Knowledge, Practices, and Effectiveness of Control Strategies Against Storage Pests and Soil Borne Diseases in Cereal and Legume Crops in Lupane District, Zimbabwe Zhou, Mandhlenkosi; Mlambo, Alec; Tibugari, Handsen
Journal of Tropical Plant Protection Vol. 7 No. 2 (2026)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2026.007.2.3

Abstract

Smallholder farmers in semi-arid sub-Saharan Africa depend largely on both formal and informal seed systems, although the contribution of various seed types towards land cultivation remains unclear. The objectives of the study were to evaluate farmer knowledge of storage pests and soil-borne diseases, seed sourcing systems, and perceived efficiency of traditional seed storage practices for cereals and legumes in Lupane District, Zimbabwe. A questionnaire survey was administered to 400 farmers across ten wards between October 2022, and March 2023 and October 2023. A multistage sampling method was adopted in ward and farmer selection. Findings indicated that despite the fact that 97.8% of respondents used seed through government input schemes and 98.8% used commercial seed at least once, the quantities were generally low for planting. As a result, most land cultivated was planted with farm-saved seed. In relation to area planted, farm-saved seed that is traditionally treated comprised 45% of land cultivated, untreated farm-saved seed comprised 42%, while commercial seed comprised only 13%. Economic factors were the key factor in the choice of farm-saved seed. Storage crop pests (maize, rice, and cowpea weevils), were well known, while there was limited knowledge on soil-borne fungal diseases. Holes and frass were identified as damage symptoms in all crops. Combretum imberbe ashes was identified as the most used and preferred traditional seed preservation treatment. Farm-saved seeds were predominant used despite the persistent use of commercial seeds, and there is need for scientific studies of common botanicals used in seed production in semi-arid farming communities.
Synergistic Insecticidal Activity of Essential Oils from Ocimum basilicum and Ocimum gratissimum Against the Cowpea Weevil, Callosobruchus maculatus Ayobami, Owolabi Tunde; Teslim, Ibrahim Oluwatomiwa; Adigun, Rafiu Toheeb; Catherine, Agu Chinwendu; Etetoghene, Omoko Awesome; Chiemezie, Okafor Izuchhukwu; Oyinlola, Dennis Olabode God’s favour
Journal of Tropical Plant Protection Vol. 7 No. 2 (2026)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2026.007.2.4

Abstract

Protecting stored cowpea from Callosobruchus maculatus remains one of the most persistent challenges faced by smallholder farmers, who depend on this staple legume for both nutrition and income. Although several insects attack stored grains, C. maculatus is widely recognized as the most destructive pest of Cowpea, causing rapid quality loss and significant yield reduction. In this study, we investigated the insecticidal, repellent, and synergistic activities of individual and blended essential oils against adult C. maculatus. Fumigation, contact toxicity, and repellency assays were conducted under laboratory conditions (27 ± 2 °C, 65 ± 5% RH, and 12:12 h (L:D) photoperiod) to assess the impact of individual oils and formulated blends on pest mortality and behavior at varying concentrations of 0.2 – 0.5% over  a period of 72 hrs. The oils varied considerably in potency. Ocimum gratissimum consistently emerged as the most effective, showing strong fumigant toxicity (48.87 ± 1.77%) and robust repellent action (100 ± 0%). Ocimum basilicum displayed moderate activity, whereas Citrus limon and Citrus sinensis produced minimal insecticidal effects (2.38 ± 1.65%) at comparable concentrations. Blended formulations, however, performed substantially better than single oils. The binary mixture FM1 exhibited the highest overall efficacy, resulting in rapid and near-total mortality (68.4 ± 1.99%), strong contact toxicity (70.0 ± 2.646%), and complete repellency within 2 hours of exposure period. Synergistic interactions were most evident in FM1, where observed effects exceeded those expected from individual components (SF; 1.94, CI; 0.005). Ternary (FM3) and quaternary (FM4) combinations also improved bioactivity, though to a lesser extent (SF; 1.73, CI; 0.045). These findings demonstrate the promise of Ocimum-based essential oil blends as eco-friendly, synergistic alternatives for protecting stored cowpea.

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