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Sain Akuatik
Published by Universitas Muhammadiyah Purwokerto
ISSN : -     EISSN : -     DOI : -
Core Subject : Education,
Education
Arjuna Subject : -
Articles 2 Documents
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Search results for , issue " Vol 12, No 1 (2009): SAINS AKUATIK VOLUME 12 NO. 1 2009" : 2 Documents clear
OPTIMASI DOSIS VAKSIN PROTEIN SITOPLASMA SEL Aeromonas hydrophila UNTUK PENGENDALIAN PENYAKIT MAS (Motile Aeromonas Septicemia) PADA GURAMI (Osphronemus gouramy Lac.) Mulia, Dini Siswani; Purbomartono, Cahyono; Wuliandari, Juli R.
Sain Akuatik Vol 12, No 1 (2009): SAINS AKUATIK VOLUME 12 NO. 1 2009
Publisher : Universitas Muhammadiyah Purwokerto

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Abstract

Penelitian ini bertujuan untuk mengungkap dua masalah, yaitu (1) mengetahui efektivitas vaksin sitoplasma sel A. hydrophila untuk pengendalian penyakit MAS pada gurami, dan (2) mengetahui dosis optimal vaksin sitoplasma sel A. hydrophila untuk pengendalian penyakit MAS pada gurami. Penelitian dilaksanakan menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan tiga ulangan, yaitu : (1) K : kontrol yang divaksin dengan larutan PBS steril, (2) D1: vaksin protein sitoplasma sel  A. hydrophila dosis 2,5 µg/ekor, (3) D2 : vaksin protein sitoplasma sel  A. hydrophila dosis 5 µg/ekor, (4) D3 : vaksin protein sitoplasma sel  A. hydrophila dosis 7,5 µg/ekor, (5) D4 : vaksin protein sitoplasma sel  A. hydrophila dosis 10 µg/ekor  Gurami yang digunakan berukuran panjang 10-12 cm dengan berat 25,8-28,5 g. Vaksinasi dilakukan secara suntikan intramuskular. Satu minggu setelah vaksinasi dilakukan vaksinasi booster, dan uji tantang dilakukan dua minggu setelah vaksinasi booster. Parameter yang diamati meliputi titer antibodi, sintasan, tingkat perlindungan relatif (Relative Percent Survival/RPS), dan rerata waktu kematian (RWK). Data dianalisis dengan analisis varian dan Duncan’s Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa vaksin protein sitoplasma sel  A. hydrophila efektif untuk pengendalian penyakit MAS pada gurami. Vaksin ini dapat meningkatkan produksi titer antibodi, sintasan, dan RPS (P<0,5) gurami. Dosis vaksin protein sitoplasma  sel  A. hydrophila optimum adalah 7,4 mg/ekor yang memberikan nilai RPS tertinggi sebesar 89,31 %. 
PENGARUH PENAMBAHAN KOMBINASI LEVAMOSIL DAN VAKSIN Aeromonas hydrophila TERHADAP PERTUMBUHAN, JUMLAH NEUTROFIL, DAN SINTASAN IKAN GURAMI (Osphronemus gouramy Lac.) Purbomartono, Cahyono; Mulia, Dini Siswani
Sain Akuatik Vol 12, No 1 (2009): SAINS AKUATIK VOLUME 12 NO. 1 2009
Publisher : Universitas Muhammadiyah Purwokerto

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Abstract

The research conducted n gouramy fish with size among 12-15 cm in length and average of 30 g in wight. the treatment used levamisol and their combination with vaccine Aeromonas hydrophila which applied by immersion during 6 hours. Methods of research used experimentally laboratories using completely randomized design with 6 replication. The fishes placed in aquaria, each of its 12 fishes. The data’s observed consisted of growth both of length and weight, number of neutrophyl, survival rate, and water quality. The aim of the research was to know the effect of levamisol and its combination with vaccine on the respons against their growth, neutrophyl responses and survival rate of gouramy fishes. The results showed, treatment of levamisol and their combination with vaccine could increase on the growth and the best result revealed on P1, as 1.2 cm and 0.68 g for their length and weight respectively. Cellular response of neutrophyl showed better reached at P3 and P4 as number of 5.3 neutrophyl cell between on day H18 and H24. Higher survival rate reached on P2 and P3. During researched, water quality was on qualified standard requirements for larvae rearing.

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