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Prosiding KPSDA
Published by Universitas Sebelas Maret
ISSN : -     EISSN : -     DOI : -
Core Subject : Science,
Earth & Planetary Sciences
Arjuna Subject : -
Articles 54 Documents
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Pengaruh Model Project Based Learning Berbasis Potensi Makroalga Daerah Pesisir Terhadap Kemampuan Berpikir Kritis Siswa Di Sman 1 Tanjungari Gunungkidul D. I. Yogyakarta Shadaika, Milyarda
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
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Abstract

This research aims to know the effect of Project Based Learning (PjBL) based on macroalgae potency of seashore toward students critical thinking skills. This research was held at SMAN 1 Tanjungsari in academic year 2013/2014. All student of grade X MIA had been chosen as a research population. Randomly, two classes were selected representing the experimental class (implementation PjBL based on macroalgae potency of seashore) and the control class. A quasi-experimental method was designed to achieve the research objectives. Data were collected during one month or four meetings consist two hours (45 min each). Critical thinking was measured through essay test which were formulated based on cognitive scale of Bloom taxonomy (C4–C6). All the data were analyzed using t-test method with α=5%. Observation of learning process was also done to get information on critical thinking process. The result showed that implementation of PjBL based on localities influenced significantly on students’ critical thinking skills.
Keanekaragaman Kapang Aspergillus pada Serasah Daun Talok (Muntingia calabura L.) di Kawasan Desa Sukolilo Barat, Kecamatan Labang, Kabupaten Bangkalan, Madura Isworo_Rukmi, Arum Krisna -
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
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Abstract

Desa Sukolilo Barat Kabupaten Bangkalan, Madura mempunyai kondisi alam yang cukup ekstrim karena suhu udara yang panas dan kering, dengan tanah yang mengandung kapur, hal ini menjadikan daerah tersebut sebagai salah satu habitat untuk mendapatkan kapang dengan sifat xerofilik dan alkalotorelan. Serasah daun merupakan salah satu substrat representatif untuk mendapatkan  kapang karena mengandung berbagai macam bahan organik, yang dapat digunakan sebagai sumber nutrisi. Pohon yang cukup dominan dikawasan tersebut adalah pohon talok (Muntingia calabura L.).  Kapang Aspergillus telah dikenal mempunyai spesies-speciesnya yang penting dalam bidang industri. Penelitian untuk  mengetahui keanekaragaman kapang dilakukan untuk mengetahui jenis dan kemampuan  ensimatis kapang yang diperoleh dan mengetahui perannya dalam siklus biogeokimiawi. Enumerasi kapang dan isolasi  dilakukan dengan metode langsung dan tidak langsung pada media agar  DG18, MEA dan OA. Kapang yang terisolasi selanjutnya diuji aktivitas enzimatisnya, meliputi uji  selulolitik, uji amilolitik dan uji proteolitik pada  suhu inkubasi 310C.  Dua puluh dua isolat kapang  Aspergillus berhasil diisolasi dari 30 sampel tanah dari 3 lokasi yang ditentukan secara purposive. Berdasarkan Indeks Keanekaragaman Shannon-Wiener menunjukkan keanekaragaman spesies yang tinggi. Aktivitas selulolitik tertinggi ditunjukkan oleh  A. tamarii (ISM 1),  A. aculeatus (ISM 10) menunjukkan aktivitas amilolitik tertinggi, sedangkan aktivitas proteolitik tertinggi ditunjukkan oleh Aspergillus  sp. 2  (ISM 17).
Kadar Total Pigmen Klorofil dan Senyawa Antosianin Ekstrak Kastuba (Euphorbia pulcherrima) Berdasarkan Umur Daun Maulid, Rendy Rohmatul
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
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Abstract

Studies on the content of the pigment chlorophyll and anthocyanin pigments in the leaves of plants kastuba at different levels of development has not been done. The leaves of this plant shows a very noticeable difference in color. At the level of developments in the oldest leaves (bottom) has a dark green color, while at the level of development of the youngest leaves (above) has a deep red color. This indicates that the difference in the color of the leaves with different levels of development shows the different types of pigments. The purpose of this study was to compare the content of the pigment chlorophyll and anthocyanin pigments in leaves kastuba (Euphorbia pulcherrima.) At different levels of development leaves.  This research was conducted by the method of extraction and maceration using 70% ethanol. The extract obtained is used to test the color stability during storage for 2 days. After the leaves are extracted with 70% alcohol, chlorophyll content was measured using a spectrophotometer at λ 649 and 665 nm and anthocyanin content was measured using a spectrophotometer at λ 510 and 700 nm. The results showed that there are differences in chlorophyll and anthocyanin content in leaves of different levels of development. Between green leaves and red leaf, the highest levels of chlorophyll pigments in leaves of green, at the level of the first leaf (leaf oldest) and the lowest in the red leaves, at the level of the third leaf (leaves most young ). Levels of anthocyanin compounds in the leaves of different age levels turned out to show different results. Between green leaves and red leaf, the highest levels of anthocyanin compounds in the leaves that are colored green, at the lowest level (the oldest) and the lowest in the leaves red, at the top level (the youngest)
Bioprospeksi dan Identifikasi Molekuler Bakteri yang Berasosiasi dengan Alga Hijau sebagai Penghasil Senyawa Antibakteri Endang_Kusdiyantini, Rizky Panji_Nugroho
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
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Abstract

Bacteria are ubiquotus and could grow in various environments, including those associated with marine organisms such as algae, sponge, sea grass and soft corals. For these organisms, bacteria help building their active defense mechanisms by producing secondary metabolites such as antibacterial compounds. This research aimed to study the potency of seaweed-associated bacteia in producing antibacterial compounds. The seaweed used were Halimeda macroloba, Caulerpa racemosa dan Ulva sp. The results of isolation found five bacteria: one from H. macroloba, three from C. racemosa, and one from Ulva sp. Antibacterial assays was done by measuring inhibition zone to the growth of three bacteria: Escherichia coli, Staphylococcus aureus, dan Pseudomonas aeruginosa. Bacteria Isolated from H. macroloba showed the highest inhibition zone towards P. aeruginosa of 18.1 mm. Using molecular 16S rRNA identification, this isolate showed similarity to Idiomarina fontislapidosi strain HME8844. Biochemical tests showed that this isolate   was negative to ferment the sugar of arabinose, fructose and sucrose, and also negative to amylum hidrolysis.
naskah awal_prosiding FKIP UNS, P. Biologi
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
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Abstract

COVER, EDITOR, SUSUNAN PANITIA, KATA PENGANTAR, SUSUNAN ACARA, JADWAL PRESENTASI MAKALAH PARALEL, DAFTAR ISI, DAFTAR PEMAKALAH,
Analisis penghambatan aktivitas mortalin dengan senyawa herbal Indonesia untuk skrening anti kanker melalui in silico UB, Widodo
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
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Abstract

The prevalence of cancer in Indonesia is still very high, especially breast and cervical cancer. However, the potent and safe drugs for cancer therapy are still not available. One strategy to find the safe cancer drug is screening active compound that able to block the function of mortalin protein. In cancer cells, mortalin inhibits p53 protein to enter the nucleus, which inhibit p53 function to stimulate apoptosis and cell cycle  arrest in cancer cells. In this study, mortalin is used as target protein to screen of active compounds from Indonesia plants. The active compounds were analysed its binding affinity to p53-binding domains of mortalin, compared to withanone as a positive control. The result suggested that four compounds, there are Hesperidin, Artoindonesianin A, glycyrrhetinic acid, and Liriodenine have better binding affinity to mortalin compared to withanone. These compounds can be isolated from Citrus aurantium, Artocarpus reticulatus, Glycyrrhiza glabra and Michelia champaca, respectively. Therefore by using the active compound as cancer drug material will preserve the plant in the nature.
Tingkat Cemaran Mikrobia pada Tanaman Biofarmaka Curcuma domestica setelah Proses Pengeringan Kusumaningrum, Hermin Pancasakti
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
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Abstract

Solar food processing is an emerging technology that provides good quality foods and economist. Traditional medicinal plants product such as turmeric (C. domestica) drying product from local industries on Gunungpati Semarang have not meet its standards quality yet, in terms of microbial contaminants. The purpose of this research is to test the drying product resulted from  heating process of simplicia by  sunlight and oven. Observations of focusing on microbial contaminant from treatment after  3 month incubation. The research method conducted by drying simplicia of  C. domestica at variant a temperature of 300C, 400C, 500C and 600C for 1, 3, 6, 9, 12, 24, and 48 hours. The results showed that drying at a temperature of 500C and 600C for 24 hours and 48 hours showed the lowest contamination and still in accordance with ISO standard for simplicia which is number of  mold and yeast <1´104 cfu, while the total plate count were <1´107cfu.
Penerapan Pendekatan Konstruktivisme Berorientasi Green Chemistry untuk Meningkatkan Keterampilan Berpikir Kritis dan Hasil Belajar Kimia SMA Astuti, Andari Puji
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
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Abstract

Learning chemistry require laboratory work to support good teaching and learning process. SMA Muhammadiyah plus Salatiga is the limited number of laboratory and chemicals are available resulting in chemistry learning process is not managed well. Critical thinking skills are underdeveloped learners and learning outcomes prior to remedial classes is low. Green chemistry approach oriented constructivism is one of the alternatives used in solving chemistry problems learning process in SMA Muhammadiyah Plus Salatiga. This approach uses nature as a learning medium. Conservation efforts in the field of education. This study aims to improve critical thinking skills and student learning outcomes. This study uses a class action research consisting of three cycles .. The results showed a significant difference to the cognitive learning and critical thinking skills in each cycle. Completeness average for cognitive learning outcomes in each cycle reaches 70%, 82% and 88%. Critical thinking skills were observed through the activities of students in the class average for each cycle is 90. More than 75% of students responded positively to the learning chemistry with green chemistry oriented constructivism approach. Learners become actively involved in the learning process and have high initiative in developing the subject in class.
Apocynoideae dan Asclepiadoideae dari Pegunungan Baturagung (Gunung Nglanggeran, Gunung Mintorogo, Gunung Parangan, Gunung Gedang, Gunung Ijo): Inisiasi Pencirian dan Konservasi (UIN), Widodo
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
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Abstract

Fenomena kepunahan jenis tumbuhan mendorong timbulnya kesadaran pentingnya usaha konservasi. Langkah eksplorasi, pendataan dan penelitian mendalam merupakan tonggak awal penentuan strategi konservasi tumbuhan. Tulisan ini mempresentasikan keragaman tumbuhan subfamili Apocynoideae dan Asclepiadoideae di Pegunungan Baturagung Yogyakarta. Sejak eksplorasi tahun 2009 telah diidentifikasi dan ditemukan sekitar 17 species dari anggota subfamili Apocynoideae dan Asclepiadoideae tersebar di Gunung Nglanggeran, Gunung Mintorogo, Gunung Parangan, Gunung Gedang, Gunung Ijo pada wilayah Pegunungan Baturagung Yogyakarta. Species-species tumbuhan tersebut pada umumnya ditemukan dalam populasi yang sangat terbatas, teramankan pada lahan-lahan liar, tebing curam, tepi hutan rakyat, lahan-lahan yang tidak dibuka untuk peladangan. Tumbuhan tersebut hampir tidak dikenali lagi identitasnya oleh masyarakat, terutama yang berhabitus bukan pohon. Informasi yang berkaitan dengan tumbuhan-tumbuhan tersebut sangat sedikit dalam literatur maupun informasi internet sehingga proses identifikasinya relatif sulit dan bahkan beberapa diantaranya memerlukan publikasi penemuan kembali, redeskripsi, pencirian dan penemuan di Jawa. Identitas dan data taksonomik tumbuhan sangat penting dalam mengawali tindakan konservasi dan pemanfaatannya.
Biolitik Enzim Bekicot (Achatina fulica) Sebagai Agen Fusi Protoplas Pichia manshurica Intraspecifik Wijanarka, Wijanarka
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
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Abstract

Enzymes were an important role both in the food or beverage industry, but there is also an enzyme that acts as a breaker of the organism or cell wall of microorganisms. The enzyme known as biolytic enzyme. Extraction enzyme taken from the snail (Achatina fulica), especially in the abdomen, as in this section contains compounds glukorononidase β, and beta-glucanase and endo arylsulphatase (Ezeronye and Okerentugba, 2001). This enzyme was an important role before the fusion process takes place, namely for protoplast isolation. Furthermore, the enzyme used to break down the cell wall of the yeast Pichia manshurica. The yeasts are indigenus located around dahlia tubers and has the ability to produce inulinase (EC 3.2.1.7). Protoplast fusion is one way to improve the strain, so hopefully we will get a new strain and superior compared to its parent. The purpose of this research was using enzymes from snail to break the cell walls of yeast (protoplast isolation) and to obtain new fusan. Protoplast isolation performed enzymatically by using enzyme biolytic  of snail. Fusion process was done by mixing the two parental pellets in solution osmotic stabilizer sorbitol solution of 1.0 mol / L containing 30% PEG 6000 (polyethylene glycol) and 10 mM CaCl2 for 20 minutes. The results showed that the concentration of biolytic 100% can be utilized as an agent biolytic enzyme and capable of producing protoplasts of 8.1 x 109 and it has been found that 3 fusan  namely were Fusan D F1, F4 and F7 D.

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