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agriTECH

agritech
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Published by Universitas Gadjah Mada

ISSN : 02160455 EISSN : 25273825 DOI : 10.22146/agritech

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Agritech with registered number ISSN 0216-0455 (print) and ISSN 2527-3825 (online) is a scientific journal that publishes the results of research in the field of food and agricultural product technology, agricultural and bio-system engineering, and agroindustrial technology. This journal is published by Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta in colaboration with Indonesian Association of Food Technologies (PATPI).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 1,392 Documents

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Potensi Protein Kecambah Kedelai dalam Menstimulasi Sekresi Insulin pada Pankreas Tikus Normal dan Diabetes Bayu Kanetro; Zuheid Noor; Sutardi Sutardi; Retno Indrati
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The objective of this investigation was to examine the potential of seed and germinated SP that were extracted at two condition precipitation, Kunitz Trypsin Inhibitor (KTI) and Bowman Birk Inhibitor (BBI) to stimulate insulin secretion of the pancreas of induced diabetic and normal rats by in vitro bioassay. Mature male Sprague Dawley (SD) rats of the in vitro bioassay were divided into two groups. They were diabetic rats that was induced by aloksan injection and normal rats (without aloksan injection). Rat was anaesthetized with ether, and the pancreas was taken out, injected by HBS solution, hydrolyzed by collagenase and then washed several times with RPMI 1640 LITE solution and Kreb Ringer bicarbonate buffer before incubation. The islets of the pancreas were incubated in Kreb buffer under seven conditions of media treatment as follows: glucose medium that was used as a reference standard (R), total protein of seed (SPT), total protein of germinated soybean (GPT), TI protein of seed (SPTI), TI protein of germinated soybean (GPTI), KTI, and BBI. After two hours of incubation, the mixture was sonicated, and centrifuged The supernatant was stored at –20 oC for determination of insulin. The insulin analysis was conducted by ELISA method. The results show that aloksan injection increased the level of blood sugar and induced diabetic rats. The media treatment altered the soluble protein profile and the insulin secretion of the islets. The germinated SP has the ability to stimulate insulin secretion of the pancreas of diabetic as well as normal rats. The insulin secretion of the islets in the medium of crude TI of germinated soybean (GPTI) was highest. The potential order of the insulin secretion of normal rats islets were GPTI, KTI, BBI, GPT, SPTI, SPT, and R media. While the potential order of the insulin secretion of diabetic rat islets were GPTI, GPT, SPTI, BBI, KTI, SPT, and R media. The potential of germinated SP to stimulate insulin secretion was better than ungerminated SP.ABSTRAKTujuan dari penelitian ini adalah mempelajari potensi protein biji dan kecambah kedelai (protein total dan TI), Kunitz Trypsin Inhbitor (KTI), dan Bowman Birk Inhibitor (BBI) dalam menstimulasi sekresi insulin pada pankreas tikus normal dan diabetes melalui pengujian biologis secara in vitro. Tikus Sprague Dawley (SD) jantan yang diguna- kan dalam pengujian biologis secara in vitro dibagi menjadi dua kelompok, yaitu tikus diabetes yang disiapkan dengan cara menginduksi melalui injeksi aloksan, dan tikus normal (tanpa diinjeksi aloksan). Selanjutnya tikus dieksekusi dengan bius ether dan dilakukan pencucian beberapa kali dengan larutan RPMI 1640 LITE. Pencucian terakhir meng- gunakan buffer Kreb Ringer Bicarbonat sebelum pancreas islet yang diperoleh diinkubasi. Pancreas islet diinkubasi dalam Kreb buffer yang dicampur dengan 7 perlakuan media inkubasi yang berbeda, yaitu medium glukosa sebagai control (R), protein total biji kedelai (SPT), protein total kecambah kedelai (GPT), protein TI biji kedelai (SPTI), pro- tein TI kecambah kedelai (GPTI), KTI dan BBI. Inkubasi dilakukan selama 2 jam, kemudian disonikasi, dan sentrifu- gasi. Supernatan yang diperoleh disimpan pada suhu -20 oC untuk disiapkan dalam analisis insulin mengggunakan metode ELISA. Hasil penelitian menunjukkan bahwa injeksi aloksan pada tikus dapat menginduksi terjadinya diabetes karena dapat meningkatkan gula darah tikus. Perlakuan media inkubasi berpengaruh terhadap kadar protein terlarut media dan kemampuannya menstimulasi islet untuk mensekresikan insulin. Protein kecambah kedelai memiliki kemam- puan menstimulasi sekresi insulin baik pada pancreas tikus normal maupun diabetes. Sekresi insulin dari islet dalam medium GPTI paling tinggi dibandingkan perlauan yang lain. Urutan potensi sekresi insulin dari pancreas tikus nor- mal, yaitu berturut-turut dari yang tertinggi GPTI, KTI, BBI, GPT, SPTI, SPT, dan R. Sedangkan pada pancreas tikus diabetes, yaitu dari yang tertinggi GPTI, GPT, SPTI, BBI, KTI, SPT, and R media. Secara umum disimpulkan bahwa potensi protein kecambah kedelai dalam menstimulasi sekresi insulin lebih baik dibandingkan protein biji kedelai.

Study of Household Biodigesters Chandra Wahyu Purnomo; Ambar Pertiwiningrum; M. Prasetya Kurniawan
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Tunnel or tubular type of bio-digester has been widely developed in many countries for producing a renewable clean fuel gas named biogas. This digester type only requires minimum maintenance and simple construction for ensuring its lower cost compared to others with similar capacity. Mostly, polyethylene (PE) plastic bag is used for construction materials of the tubular digesters. In this research, the tubular type has been constructed using large diameter PVC (Poly Vinyl Chloride) pipe for increasing the durability and safety. The performance of PVC digesters have been tested by measuring biogas production rate and CH concentration in the gas. COD (Chemical Oxygen Demand) and BOD (Biological Oxygen Demand) drop between inlet and outlet sludge have been measured for examining the digester ef- ficiency. Then, the performance was compared with PE plastic digesters data from several literatures which still needs some improvements for enhancing its capability and performance.

Efek pH dan Konsentrasi Butirat Anhidrida selama Butirilisasi Pati Garut Damat Damat; Haryadi Haryadi; Y. Marsono; Muhammad Nur Cahyanto
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Several characteristics of native starch have limited utilizations. Chemical modification improves the physical, chemi- cal, and functional properties of starch and therefore it extends the utilization. The aim of this research was to inves- tigate the effect of the dispersion of pH (8, 10 and 12) and butyrate anhydride concentration (5, 10, 15, 20 and 25 %) on the butyrilization process of arrowroot starch. The modified (butyrated) starch was analyzed for its butyril group, degree of substitution (DS), swelling power, solubility, amylography, and transmittance. The result indicated that the maximum butyriilation was achieved at pH 10 and addition of 20 % butyrate anhydride. The higher DS resulted in more transparent starch paste, the more stable viscosity and the higher swelling power.ABSTRAKPati garut, sebagaimana jenis pati alami lainnya diketahui memiliki kelemahan sifat fisik dan kimia yang menyebabkan penggunaan pati garut pada industri pangan relatif terbatas. Untuk itu maka perlu dilakukan perbaikan sifat fisik dan kimianya dengan cara melakukan modifikasi pati garut. Penelitian ini dilakukan dengan tujuan untuk mendapatkan pengaruh pH dispersi (8, 10 dan 12) dan konsentrasi butirat anhidrida (5, 10, 15, 20 dan 25 %) pada proses butirilisasi pati garut. Selain itu, penelitian ini juga dilakukan untuk mengetahui karakteristik pati-garut butirat, yang meli- puti persen butiril, derajat substitusi, daya mengembang, kelarutan, sifat amilografi dan kejernihan pasta pati-garut butirat. Hasil penelitian menunjukkan bahwa butirilisasi maksimum dicapai pada pH 10 dengan konsentrasi butirat anhidrida 20 %. Pati butirat derajat substitusi yang tinggi memiliki kejernihan pasta, daya mengembang yang lebih tinggi dan viskositas yang lebih stabil, sedangkan kelarutan lebih rendah.

Pengaruh Varietas Apel dan Campuran Bakteri Asam Asetat terhadap Proses Fermentasi Cider Dessi Caturryanti; Sri Luwihana; Siti Tamaroh
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The objectives of the research were evaluate apple variety and acetic acid bacteria ratio used in cider fermentation. Two apple varieties (Manalagi and Rome Beauty) were used and mixed culture of two bacteria species (Acetobacter pasteurianus INT-7 and Acetobacter aceti JCM 7640) were used for inoculation. Apple extract was inoculated by ratio of acetic bacteria 1:1 and 1:2, and etanol substrat of 5 % was added respectivelly, continued aerobic condition at room temperature for 7 days. The result of the reseach indicated that cider fermentation using Rome Beauty variety with mixed culture of A. pasteurianus INT-7 : A. aceti JCM 7640 = 1:2 produced acetic acid 3.11 %, product yield 0.85 g/g and efficiency 60.56 %.ABSTRAKTujuan penelitian ini adalah untuk mengevaluasi pearan varietas apel dan rasio bakteri asam asetat dalam fermen- tasi cider. Dua varietas apel (Manalagi and Rome Beauty) dan dua kultur bakteri (Acetobacter pasteurianus INT-7 and Acetobacter aceti JCM 7640) digunakan dalam penelitian ini. Ekstrak buah apel dinokulasi dengan bakteri asam asetat dengan rasio 1:1 dan 1:2. Sebagai substrat ditambahkan ke dalam medium fermentasi etanol sebanyak 5 %. Fermentasi silakukan secara aerobik pada suhu ruang selama 7 hari. Hasil penelitian menunjukkan bahwa fermentasi cider menggunakan varietas apel Rome Beauty dengan perbandingan A. pasteurianus : A. aceti = 1:2 menghasilkan asam asetat sebanyak 3,11 %, yield produk 0,85 dan efisiensi sebesar 60,56 %.

Komponen Kimia dan Anatomi Tiga Jenis Bambu dari Seram, Maluku Mery Loiwatu; Elyazar Manuhuwa
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

As a versatile plant, the basic properties of bamboo are various, depending on species, site, and position inside the stem. This study was conducted at three sites (Buria, Morekao, Tala), on three species (Dendrocalamus asper, Schizos- tachyium brachycladum, Schyzotachium lima), and three positions of the stem (base, middle, top). Objective of the study was to measure chemical component and anatomical feature of the three species of bamboos of West Ceram’s Sub-district (Taniwel, Piru, Kairatu) and three parts of stem (base, middle, top). Factorial experiment in split plot design was applied in the study with 3 replications. Result of the study indicated that there was a significant effect of location on the lignin content, diameter of the fiber and fiber cells lumen, and proportion of parenchyma cells; species of bamboo to the length and diameter of fiber cells; and portion of stem to the extractive soluble in hot water, soluble in alcohol benzene, length and diameter of fiber cells, and diameter of fiber lumens. Extractive soluble in cold water was 3.10-3.79 %; hot water was 5.43-6.23 %; alcohol benzene was 3.37-4.10 %; alpha cellulose was 44.22-46,94 %; holocellulose was 71.97-75.57 %; lignin was 26.00-27.37 %; length of fiber cell was 3.40-3,96 mm; diameter of fiber cell was 4.34-4.91 micron; diameter of fiber cell lumens was 2.74-3.23 micron; cell wall thickness of fiber cell was0.76-0.91 micron; proportion of parenchyma cells was 51.95-56.85 %; proportion of fiber cells was 27.81-62.66 %;and proportion of pores was 12.39-14.60 %.ABSTRAKSebagai tanaman serbaguna, sifat dasar bambu bervariasi menurut jenis, tempat tumbuh dan posisi didalam batang. Penelitian dilakukan pada 3 lokasi (Buria, Morekao, Tala), terhadap 3 jenis bambu (Dendrocalamus asper, Schizos- tachyium brachycladum, Schyzotachium lima), dan 3 posisi dalam batang (pangkal, tengah dan ujung). Tujuan pene- litian adalah untuk mengukur komponen kimia dan anatomi 3 jenis bambu (Dendrocalamus asper, Schizostachyium brachycladum, Schyzotachium lima) asal 3 kecamatan Seram bagian barat (Taniwel, Piru, Kairatu), dan 3 bagian batang (pangkal, tengah dan ujung). Percobaan faktorial dalam rancangan petak terpisah digunakan dalam penelitian dengan 3 ulangan. Hasil penelitian menunjukkan pengaruh signifikan lokasi terhadap kadar lignin, diameter sel serat dan rongga sel serat dan proporsi sel parensim; jenis bambu terhadap panjang dan diameter sel serat; posisi dalam batang terhadap kadar ekstraktif larut air dingin, ekstraktif larut alkohol benzen, panjang dan diameter sel serat, dan diameter sel serat. Ekstraktif larut air dingin berkisar 3,10-3,79 %; larut air panas 5,43-6,23 %; larut alkohol benzen3,37-4,10 %; alfa selulosa 44,22-46,94 %; holoselulosa 71,97-75,57 %; lignin 26,00-27,37 %; panjang sel serat 3,40-3,96 mm; diameter sel serat 4,34-4,91 mikron; diameter rongga sel serat 2,74-3,23 mikron; tebal dinding sel serat0,76-0,91 mikron proporsi sel parensim 51,95-56,85 %; proporsi sel sel serat 27,81-62,66 %; dan proporsi sel pori12,39-14,60 %.

Aktivitas Antioksidasi Ekstrak Fenol Umbi Sarang Semut (HYDNOPHYTUM SP.) Pada berbagai Suhu Penyeduhan Yohana S.K. Dewi; Dominika Dominika
agriTECH Vol 28, No 2 (2008)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Antioxidant activity of Sarang Semut tubers (Hydnophytum sp.) extracts toward stepping treatments was investigated. Powder of sarang semut tubers was diluted in 200 ml of distilled water then its being subjected to stepping treatment of three different treatments: cooked at 60, 80 and 100 °C for 2, 4 and 6 minutes. Effect of stepping treatment of Sarang Semut tuber juices on the antioxidant activity and the total phenol contents was investigated. Antioxidant activity of Sarang Semut tuber juices was estimated according to DPPH methods. The total phenol content in the tuber juices was measured with Folin-Ciocalteu reagent using gallic acid as a standard. The results showed that stepping treatment did not affect antioxidant activity of Sarang Semut tuber juices. Stepping treatment of Sarang Semut tuber juices at 100 °C exhibited the highest total phenol contents.ABSTRAKAktivitas antioksidasi ekstrak umbi sarang semut (Hydnophytum sp.) telah diteliti pada berbagai suhu penyeduhan. Serbuk umbi sarang semut (2,5g) diseduh dalam akuades yang telah dipanaskan pada suhu 60,8 dan 100 °C selama 2,4, dan 6 menit. Pengaruh perlakuan penyeduhan terhadap serbuk sarang semut diuji aktivitas antioksidasi dan kand- ungan total fenol. Pengujian aktivitas antioksidasi dengan metode DPPH dan kandungan total fenol dengan metode Folin Ciocalteou dengan standar asam galat. Hasil penelitian menunjukkan perlakuan penyeduhan tidak mempengar- uhi aktivitas antioksidasi serbuk sarang semut. Suhu penyeduhan 100 °C menghasilkan kandungan total fenol umbi sarng semut tertinggi.

Pengaruh Pelapis dapat Dimakan dari Karagenan terhadap Mutu Melon Potong dalam Penyimpanan Dingin Doddy Andy Darmajana; Nok Afifah; Enny Solihah; Novita Indriyanti
agriTECH Vol 37, No 3 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Recently, fresh-cut melon consumption has been increasing due to the health benefits. One of methods to reduce the decay of quality by minimal processing of fruits is edible packaging. This method may extend the shelf-life of fresh-cut melon. The objective of this research was to study the effectiveness of the edible coatings and edible films to maintain the quality of fresh-cut melon during storage at 10°C for 6 days. The edible solutions were prepared by dissolving carrageenan of 2%, glycerol of 1%, and tween 80 of 0.2% into aquadest. These solutions were used as coating systems by dipping and wrapping method. Uncoated fruits served as controls. Quality was measured in terms of weight loss, hardness, soluble solids, pH value, and total plate count. The result revealed that for all treatments the value of weight loss, soluble solid content, and total plate count increased during storage whilst hardness, pH, decreased. In terms of microbiological, the coating improved the shelf-life of fresh-cut melon (up to 2 days) compared to the controls. ABSTRAKKonsumsi buah melon potong segar meningkat beberapa tahun terakhir karena manfaat kesehatannya. Salah satu alternatif terbaru untuk mengurangi penurunan kualitas yang disebabkan oleh pengolahan minimal buah-buahan adalah pengembangan edible packaging (kemasan dapat dimakan). Mereka diharapkan dapat memperpanjang umur simpan buah melon potong. Tujuan penelitian untuk mempelajari efektivitas edible coating dan edible film untuk menjaga kualitas buah melon potong segar selama penyimpanan pada temperatur 10oC selama 6 hari. Larutan edible dibuat dengan melarutkan 2% karagenan, 1% gliserol, dan 0,5% tween 80 dalam aquadest pada temperatur 70-80 °C. Larutan ini digunakan sebagai sistem pelapisan dengan metode pencelupan dan metode pembungkusan. Buah melon tanpa pelapis disiapkan sebagai kontrol. Pengukuran kualitas buah melon meliputi susut bobot, kekerasan, total padatan terlarut, nilai pH, dan total plate count. Hasil penelitian menunjukkan bahwa untuk semua perlakuan terjadi peningkatan susut bobot, total padatan terlarut, dan angka lempeng total selama penyimpanan, sementara kekerasan dan pH mengalami penurunan. Dalam hal mikrobiologi, pelapisan meningkatkan masa simpan buah melon potong (hingga 2 hari) dibandingkan dengan kontrol.Kata kunci: Karagenan; edible coating; edible film; melon

Karakterisasi Kimia dan Efek Hipoglikemik Beras Analog Berbahan Dasar Jagung, Sorgum, dan Sagu Aren Slamet Budijanto; Yanica Ivory Andri; Didah Nur Faridah; Santi Noviasari
agriTECH Vol 37, No 4 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Rice analogue is an alternative food similar to rice which is made from other sources of carbohydrate apart from rice. This research was aimed to determine the glycemic index (GI) value and chemical characteristic (total phenolics content and dietary fiber) of rice analogues made from: (1) corn, sorghum, and arenga starch (rice analogue A): (2) corn and arenga starch (rice analogue B). GI was tested in human blood samples and was determined by the comparison of curve area between food sample (analogues rice) and standard (25 g glucose) which was equivalent to 25 g of carbohydrates. The GI values of Rice analogue A and B were 47,09 and 52,31 respectively. Both were considered as low GI foods. The low GI values were due to the phenolic compounds and dietary fiber contained in the rice analoguesAnalogue rice was an alternative food similar to rice which is made from carbohydrate sources beside of paddy. This research were aimed to determine the glycemic index (GI) value and to quantify chemical characterization (total phenolics content and dietary fiber) of analogues rice made from 1) corn, sorghum, and arenga starch (analogue rice A) and 2) corn and arenga starch (analogue rice B). GI was tested by human blood samples and determined by area under the curve for test food (analogues rice) which is equivalent to 25 grams of carbohydrates and area under the curve for the reference food (25 grams of glucose) compared. Analogue rice A has 47.09 GI value, whilst analogue rice B has 52.31 GI value. Both were grouped into low GI food. A low GI value was due to the phenols compounds and dietary fiber contained in the analogues rice. ABSTRAKBeras analog merupakan pangan alternatif mirip beras yang dibuat dari sumber karbohidrat selain padi. Penelitian ini bertujuan untuk menentukan nilai indeks glikemik (IG) dan karakterisasi kimia (total fenolik dan serat pangan) pada beras analog berbahan baku: (1) jagung, sorgum, dan sagu aren (beras analog A); (2) jagung dan sagu aren (beras analog B). Pengujian IG menggunakan sampel darah manusia dan ditentukan dengan membandingkan luas area kurva antara sampel pangan (beras analog) dan standar (25 g glukosa) yang setara dengan 25 g karbohidrat. Beras analog A memiliki nilai IG sebesar 47,09, sedangkan beras analog B memiliki IG sebesar 52,31. Kedua beras analog termasuk dalam kategori pangan IG rendah. Rendahnya nilai IG dikarenakan adanya komponen fenol dan serat pangan yang terkandung pada beras analog.

Pemodelan Kinetika Pengeringan Beberapa Komoditas Pertanian Menggunakan Pengering Inframerah Nok Afifah; Ari Rahayuningtyas; Seri Intan Kuala
agriTECH Vol 37, No 2 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The drying kinetics study was carried out on agricultural commodities using an infrared dryer at temperature 60 °C. The aim of this study was to determine a drying model and effective moisture diffusivity that showed the drying characteristics of cassava slices, banana slices and groundnut pods. The experiments were conducted at three agricultural commodities (cassava, banana, and groundnut) at two capacity levels of 12 kg and 24 kg. Drying was done by arranging the agricultural material on tray of 0.5 kg and 1 kg for the treatment of 12 kg capacity and 24 kg capacity, respectively. A total of 24 trays were inserted into the dryer and every hour sample was taken to analyze the water content by gravimetric method. Data was fitted to four drying models and non-linear regression analysis was used to determine model parameters. The models were compared based on their coefficients of determination (R2) between the experimental and predicted moisture ratios. The results showed that the increase of drying capacity would decrease the drying rate for all agricultural commodities. The Page model was showed to the best for describing the drying behaviour for these agricultural products. Effective moisture diffusivity ranged between 1.34x10-10 m2/s - 3.23x10-10 m2/s. ABSTRAKStudi kinetika pengeringan telah dilakukan pada beberapa komoditas pertanian menggunakan pengering inframerah pada temperatur 60 °C. Penelitian ini bertujuan untuk menetapkan model pengeringan dan effective moisture diffusivity yang menunjukkan karakteristik pengeringan irisan singkong, irisan pisang dan polong kacang tanah. Perlakuan yang digunakan adalah jenis komoditas pertanian (umbi singkong, buah pisang, dan polong kacang) pada dua level kapasitas 12 kg dan 24 kg. Pengeringan dilakukan dengan menata bahan pertanian di atas loyang masing-masing sebanyak 0,5 kg dan 1 kg untuk perlakuan kapasitas 12 kg dan 24 kg. Sebanyak 24 loyang dimasukkan ke dalam pengering dan setiap jam diambil sampel untuk dianalisa kadar airnya dengan metode gravimetri. Data diujikan dengan empat model pengeringan dan analisis regresi non-linear digunakan untuk menentukan parameter model. Nilai rasio kandungan air eksperimental dan hasil prediksi model selanjutnya dibandingkan berdasarkan koefisien determinasi (R2). Hasil penelitian menunjukkan bahwa kenaikan kapasitas pengeringan akan menurunkan laju pengeringan untuk semua komoditas pertanian. Model Page terlihat paling baik untuk menggambarkan perilaku pengeringan bahan-bahan pertanian tersebut. Nilai difusivitas efektif kandungan air berkisar pada 1,34x10-10 m2/detik - 3,23x10-10 m2/detik.

Ekstraksi Komponen Bioaktif Daun Alpukat dengan Bantuan Ultrasonik pada Berbagai Jenis dan Konsentrasi Pelarut I Wayan Rai Widarta; I Wayan Arnata
agriTECH Vol 37, No 2 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Avocado leaves contain high bioactive components that can be utilised as a source of antioxidants. The purpose of this research was to obtain the appropriate solvent types and concentration to recover high level of bioactive compounds and high antioxidant activity from the avocado leaves’ extract. This study was conducted in two stages. The first stage was the determination of the solvent types and concentration in producing the extract of avocado leaves with the highest antioxidant activity. The research was designed using complete randomised factorial design with the type of solvent (methanol, ethanol, acetone) as the first factor and the second factor was the solvent concentration (30%, 50%, 70%). The second stage of this research was the IC50 determination of avocado leaves which has highest antioxidant activity. The result showed that the appropriate solvent used in obtaining the extract of avocado leaves with the highest antioxidant activity was 70% ethanol. The total contents of phenolic, flavonoids, tannins, and DPPH radical inhibition activity of this extract were 23.28 mg/g materials, 93.97 mg/g materials, 9.47 mg/g materials and 90.80%, respectively. Whereas, IC50 value measured using DPPH, Fe2+ chelating, and reducing power were 1870 mg/L, 1180 mg/L and 85.24 mg/L, respectively. ABSTRAKDaun alpukat mengandung komponen bioaktif yang tinggi sehingga dapat dimanfaatkan sebagai sumber antioksidan. Tujuan penelitian ini adalah mendapatkan jenis dan konsentrasi pelarut yang tepat untuk menghasilkan komponen bioaktif dan aktivitas antioksidan yang tinggi dari ekstrak daun alpukat. Penelitian dilakukan dalam dua tahapan. Pada tahap I dilakukan penentuan jenis dan konsentrasi pelarut untuk menghasilkan ekstrak daun alpukat dengan aktivitas antioksidan tertinggi. Rancangan yang digunakan adalah rancangan acak lengkap pola faktorial dengan faktor I adalah jenis pelarut (metanol, etanol dan aseton) dan faktor II adalah konsentrasi pelarut (30%, 50%, dan 70%). Pada tahap II dilakukan penentuan IC50 dari ekstrak daun alpukat yang memiliki aktivitas antioksidan tertinggi. Hasil penelitian menunjukkan bahwa pelarut yang tepat digunakan untuk memperoleh ekstrak daun alpukat dengan aktivitas antoksidan yang tertinggi adalah etanol 70% dengan kadar total fenolik, flavonoid, tanin, dan aktivitas penghambatan radikal DPPH masing-masing adalah 23,28 mg/g bahan, 93,97 mg/g bahan, 9,47 mg/g bahan, dan 90,80%. Sementara itu, nilai IC50 baik yang diukur dengan metode DPPH, pengkelatan Fe2+ maupun reducing power masing-masing adalah 1870 mg/L, 1180 mg/L, dan 85,24 mg/L.

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
Fiametta Ayu Purwandari

Contact Email
fiametta@ugm.ac.id

Phone
+6285712601130

Journal Mail Official
agritech@ugm.ac.id

Editorial Address
Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Fiametta Ayu Purwandari

Contact Email fiametta@ugm.ac.id

Phone +6285712601130

Journal Mail Official agritech@ugm.ac.id

Editorial Address Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia

Location Kab. sleman, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Fiametta Ayu Purwandari

Contact Email
fiametta@ugm.ac.id

Phone
+6285712601130

Journal Mail Official
agritech@ugm.ac.id

Editorial Address
Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA