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CDHA Ceramic Microspheres for Periodontitis Treatment: Synthesis, Characterization and Doxycycline Release Profiles Sunita Prem Victor; TS Sampath Kumar
The Indonesian Journal of Dental Research Vol 1, No 2 (2011)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

The present study is focused on the development of doxycycline loaded calcium–deficient hydroxyapatite (CDHA) microspheres for the treatment of periodontitis. The CDHA microspheres were formed by liquid immiscibility effect using gelatin and paraffin oil with varying Ca/P ratios using calcium hydroxide and diammonium hydrogen orthophosphate as precursors. The morphology of the microspheres as characterized by SEM was optimized by varying the gelatin content. The doxycycline incorporation and its release profiles were studied by UV-Visible spectroscopy in phosphate buffer at physiological conditions. The pH of the buffer solution was initially optimized to have maximum amount of drug loading. Doxycycline loading around the physiological pH of 7 has the highest amount of drug incorporation. All the microspheres exhibit similar release profiles with an initial gradual increase reaching a maximum value and then nearly constant release. The microspheres formed using 6% gelatin shows maximum amount of drug release of 80%.

The Effect of Calcium Hydroxide on Fibroblast Cells Viability Vincentia Adya Paramitta; Tetiana Haniastuti Heni; Susilowati Susilowati
The Indonesian Journal of Dental Research Vol 1, No 2 (2011)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Calcium hydroxide [Ca(OH)2] is widely used as medicament in dental pulp and root canal therapy. Previous studies demonstrate the ability of calcium hydroxide to induce necrosis in dental pulp tissue. However, the mechanism of tissue destruction remains unknown. The aim of this study was to investigate fibroblast cell viability in response to calcium hydroxide exposure. In this study, Vero fibroblast cell line was treated with various concentrations of calcium hydroxide for 24 hours. Cell viability was measured by using MTT assay. Our results showed significant decrease in cell viability after exposed with calcium hydroxide at concentration 62.5 and 125 µg/ml. The result indicated that calcium hydroxide induced cell death in Vero cell line in a dosedependent manner. This study suggests that fibroblast cell death may involved in the mechanism of pulp tissue necrosis caused by calcium hydroxide

Effect of Allicin for Re-epithelialization During Healing in Oral Ulcer Model Renny Ayu Novianty; Bernadetta Esty Chrismawaty; Goeno Subagyo
The Indonesian Journal of Dental Research Vol 1, No 2 (2011)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Allicin (diallyl thiosulfinate) is the main component of fresh garlic with multi-bioactivities as therapeutic agent. Recently, allicin is being proposed as alternative therapy for aphthous ulcer. The purpose of this study was to determine the effect of topical allicin in aqueous garlic extract on re-epithelialization during healing of acetic acid induced oral ulcer model. Thirty males SD rats aged 2 months were equally divided into two groups as experimental and control groups. After sedated with 0.2 ml intramuscular Ketamine injection, ulcer model was made by applying 99.5% glacial acetic acid-moistened paper disc on rat buccal mucosa. This would gene- rate an immediate necrotic tissue, which produced a single crateriform ulcer in each of the experimental rats for the next two days. In the experimental group, one drop of allicin was applied twice daily onto the lesion. Three rats in each group were sacrificed on days 2, 3, 6, 9 and 12 after induction and were recorded as H0, H1, H4,H7 dan H10 groups, respectively. The tissue samples from buccal mucosa were processed for HematoxylinEosin (HE) staining, and then evaluated histologically. Re-epithelialization was assessed by measuring the thickness of the epithelium using an ocular micrometer under a light microscope. Data were analyzed statistically by Independent t-test with significant p-values of <0.05 (95%). Based on histological findings and trend graphs, it can be concluded that the topical application of allicin can accelerate re-epithelialization of ulcer healing process. This study suggests that allicin in aqueous garlic extract could be developed as therapeuticagent for aphthous ulcer

Analysis of Patients Visiting Niigata University Medical and Dental Hospital with Chief Complaints of Metal Allergy and/or Focal Infection in the Previous 8 Years Yosuke Akiba; Ken Tomizuka; Masaru Kaku; Maiko Kawasaki; Masako Nagasawa; Ryohei Takano; Katsumi Uoshima
The Indonesian Journal of Dental Research Vol 1, No 2 (2011)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Dental metal allergy and dental focal infection are possible causes of dermatological diseases, but have been the subjects of few reports to date. We have been treating such patients in our special clinic for more than 20 years.The purpose of the present study was to investigate the mouths of patients visiting our dental hospital over an 8-year period, with the aim of clarifying whether dental metal allergy and/or dental focal infection affects their dermatologic conditions.We surveyed all clinical records of the 185 patients who visited Niigata University Medical and Dental Hospital with chief complaints of dental metal allergy since 2002. Diagnostics of skin diseases, periodontal records, periapical lesions, dental caries, dental metal series patch test results and Electron Probed Micro-Analysis (EPMA) data were investigated. Ninety-two (49%) patients were suffering from pustulosis palmaris et plantaris and 20 (11%) patients had lichen planus. Eighty-two (49%) patients showed positive reactions on patch testing. Based on the result of patch tests, Ni showed the highest positivity rate (62%, 51 patients), but on EPMA, the number of patients with Ni as an allergen was 14 (27%). On the other hand, more than 98% of patients who showed positive reactions on patch test to Pd and Au had these metals in their dental prostheses. In addition, 112 (60%) patients showed the possibility of dental focal infections

The Effect of Visible Light Cure (VLC) Exposure to Gingival Tissue’s Sprague dawley Rats Kwartarini Murdiastuti; Suryono Suryono; Aini Moeljono; Mefi Priba Sari; Rani Gamawati
The Indonesian Journal of Dental Research Vol 1, No 2 (2011)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Visible Light Cure (VLC) is a blue light used in dentistry as an activator for restorative material and fixed orthodontic bonding. The wavelength of VLC is between 400-500 nm and considered non-ionizing radiation that can produce free radicals. According to previous research, the light at wavelength < 500 nm could inhibit cells mitosis, cause cells damage, and reduce cells growth and inflammation. The purpose of this study was to investigate the effect of VLC exposure on gingival epithelial thickness, total neutrophil and macrophage count of gingival connective tissue of Sprague dawley rats. The subjects of this study consisted of 20 Sprague dawley rats, in 2-3 months of age and divided into 4 groups. Each group was 5 rats. The rats in each group were sacrificed before (0 day, as group A) and after 1st (group B), 3rd (group C), 5th (group D) day of VLC exposure, respectively. The exposure of VLC was done in labial aspect of cervical anterior teeth of mandible. The distance of exposure was as thick as 2 layers of celluloid strip and the histological specimens were stained by Hematoxylin Eosin. Each specimen was measured for its gingival epithelial thickness by using a micrometer and the number of neutrophil and macrophage were counted. The data of gingival epithelial thickness from 4 groups were analyzed by Kruskal Wallis. The number of neutrophil and macrophage were analyzed by u sing one way ANOVA. The results of this study showed that there were significant differences among groups on the thickness of gingival epithelial, the number of neutrophil and macrophage in the gingival connective tissue of Sprague dawley rats. The result of this study indicated that VLC exposure might decrease the thickness of gingival epithelial but increase the number of neutrophil and macrophage of gingival connective tissue of Sprague dawley rats.

Remodeling Capacity of Femoral Bone Defect by POP-CHA Bone Substitute: A Study in Rats’ Osteoclast (First Series of POP-based Bone Graft Improvement) Steven Kumar; Anne Handrini Dewi; Dyah Listyarifah; Ika Dewi Ana
The Indonesian Journal of Dental Research Vol 1, No 2 (2011)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Reconstruction of large bone defects caused by trauma, excision of tumors, and congenital malformations can be very difficult to perform. Bone engineering offers an option to improve bone reconstruction procedures. This interdisciplinary field applies the principles of biology and engineering to the development of functional substitutes for damaged bone. Our research aimed to find the ideal scaffold for bone regeneration, focusing on Calcium and Phosphate combination. In this study, Plaster of Paris (POP) was combined with CHA and implanted in femoral condyles of rats. According to the experimental result, it can be concluded that there was no significant difference in response to the implantation of POP and POP-CHA in Sprague Dawley rat femur condyle (p<0.05). It can be stated that both POP-CHA and POP shows similar trait in bone healing.

Oral Health Status Among Schoolchildren: Does Partnership With School of Dentistry Make A Difference? Sri Widiati
The Indonesian Journal of Dental Research Vol 1, No 2 (2011)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

The purpose of this study was to evaluate whether the partnership between 26 primary schools with the Faculty of Dentistry, Universitas Gadjah Mada, during the past 12 years improved oral health status of the schoolchildren. A sample survey was carried out, involving 106 fifth and sixth graders from 9 schools which have been participating in the partnership and 90 schoolchildren from non-participating schools. They were examined by trained dental students. Oral health status was represented by OHI-S and DMF-t measurement. Knowledge and attitude of oral health were measured using structured questionnaires. The study indicated that the means of OHI-S and DMF-t among schoolchildren participating in the partnership were 0.11 and 0.01 lower respectively compared to those of their counterparts, although the differences were not significant statistically. The knowledge and attitude among schoolchildren in the partnership were 2.49, 4.18, and 3.86 higher, all were highly significant (p < 0.001). Path analyses showed that the partnership reduced OHI-S and DMF-t with an overall path coefficients – 0.086 and -0.076 respectively. Although knowledge was associated with DMF-t and attitude with OHI-S, there were other unmeasured variables which were more strongly associated with oral health status of the schoolchildren.

Gender Dependence In Mouth Opening Dimensions In Normal Adult Malaysians Population Ramizu Shaari; Teoh Eng Hwa; Shaifulizan Abdul Rahman
The Indonesian Journal of Dental Research Vol 1, No 2 (2011)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

While measurement of mouth opening is an important clinica examination in diagnosis and management of oral disease, data on non-Western populations are limited. This study was therefore conducted to determine the range of mouth opening in normal Malaysian male and female adults. A total of 34 dental students of Universiti Sains Malaysia (USM) were chosen randomly and their maximum mouth opening was measured after being asked to open their mouth sufficiently to accommodate three fingers. Measurement was performed from the edge of the upper incisor to the lower incisor using a caliper divider. The difference of median values between male (47.6 mm) and female (40.8 mm) were significant respectively (p<0.05). Thus the width of mouth opening in Malaysian student population is gender dependent although further study with a larger sample size and with other ethnic groups should be carried out, focusing on age.

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Abstract

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Contact Name
-

Contact Email
-

Phone
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Journal Mail Official
-

Editorial Address
-

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA