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Biocelebes
Published by Universitas Tadulako
ISSN : -     EISSN : 25805991     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 297 Documents
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PERTUMBUHAN MISELIUM Trichoderma sp. PADA LIMBAH CAIR TEMPE DAN LIMBAH AIR KELAPA Juliana Juliana; Umrah Umrah; Asrul Asrul
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The purpose study was to determine potential tempe industry liquid waste and coconut water waste as medium propagation miselium Trichoderma sp. The treatment was conducted based on completely randomized design, consisting of seven treatments and three replication, namely: P0 (control), P1 (tempe industry liquid waste 100%), P2 (tempe industry liquid waste 80% + coconut water waste 20%), P3 (tempe industry liquid waste 60% + coconut water waste 40% ), P4 (tempe industry liquid waste 40% + 60% coconut water waste), P5 (tempe industry liquid waste 20% + 80% coconut water waste), P6 (100% coconut water waste). Variable observation included are media pH formula and the weight of mycelium biomass Trichoderma sp. The results showed that Trichoderma sp. Can be grown on all treatments except treatments P0 (control). The treatments produced in the highest mycelium biomass is P3 : 1595,333 mg per 100 mL medium, and this not significantly different from P2 : 1566,667 mg, but significantly different with all treatments.
Pengamatan Oncobasidium theobromae secara Makroskopis dan Mikroskopis, serta Gejala Serangan Sebagai penyebab Penyakit Vascular Streak Dieback (VSD) pada Tanaman Kakao di Kabupaten Sigi, Sulawesi Tengah Sitti Ardianti; Umrah Umrah; Asrul Asrul
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Oncobasidium theobromae are fungi that causes disease Vascular Streak Dieback (VSD) on the cocoa plants which can causes a totaly death, because the mechanism attack which systemic. The purpose of this study was to observe the morphology of O. theobromae macroscopically and microscopically, as well as symptoms of VSD attack on the cacao plants. This research was conducted from March to August 2016 in the Laboratory of Biotechnology Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University (FMIPA UNTAD). The result of macroscopic observation showed that O. theobromae have white mycelium on the early growth, then the color change to cream and texture like cotton. In microscopic observation, it has branched mycelium and irregular septate. VSD attack symptoms characterized with the leaf has necrosis, the former sitting leaf and the leaf base has three dots, twigs become  toothless and the ends of twigs become dry.
INVENTARISASI JENIS-JENIS RHODODENDRON (ERICACEAE) PADA HUTAN PEGUNUNGAN TAMAN NASIONAL LORE LINDU (TNLL) SULAWESI TENGAH Masnawati Masnawati; Ramadhanil Pitopang; Samsurizal Suleman
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

A research entitled  “Inventory of Rhododendron (Ericaceae) at the Montane Forest of Lore Lindu National Park (LLNP) Central Sulawesi has been conducted from March to August 2016. The Research was located at two locations namely: path of Mt. Nokilalaki (1065-2355 m asl) and path of Mt Rorekautimbu (1699 -2070 m asl)  used survey method by using the botanical exploration methods. All Rhododendron species  were collected in sets of at least two duplicates. Plant collecting was according to the “Schweinfurth method” (Pitopang et al, 2011). Processing of the specimens was conducted at the Laboratory of Biodiversity Department of Biology, Faculty of Mathemathics and Natural Sciences Tadulako University. Identification was done in the field, in CEB, and the Herbarium Bogoriense (BO), Cibinong West Java. Vouchers were deposited in Laboratory of Biodiversity Department of Biology, Faculty of Mathemathics and Natural Sciences Tadulako University. The results showed that there were four (4) species of Rhododendron from the area of study namely Rhododendron malayanum Jack, Rhododendron quadrasianum S. Vidal var. celebicum J.J.Sm. (Nokilalaki), Rhododendron zollingeri J.J.Sm. and Rhododendron celebicum DC (Rore Kautimbu).
APLIKASI BIOKOMPOS “TRICHOSUBUR PROTECT” DAN PENGARUHNYA TERHADAP BERAT UMBI BAWANG MERAH (Allium ascalonicum L) VARIETAS LEMBAH PALU Nurzakia Nurzakia; Umrah Umrah; Abdul Rahim Thaha
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research of the test of bio-compost effectiveness “Trichosubur Protect” to the red onion varieties growth (Allium ascalonicum L) in Palu valley has been conducted since from November 2015- to merch 2016. It was located in Hortus Botanicus land area (Botanical Park) departemen of Biology, Mathematics and Natural Sciences Faculty of Tadulako University. This research was designed through complete randomized design that consisted of 5 treatments and 3 repetitions. The treatmen was planting media (19 kg soil mix + 1 kg paddy husk) through bio-compost application “Trichosubur Protect” : P1 (planting media + 10 g “Trichosubur Protect”), P2 (planting media + 20 g “Trichosubur Protect”), P3 (planting media + 30 g “Trichosubur Protect”), P4 (planting media + 40 g “Trichosubur Protect”).The Parameters include the observation of leaf weight at harvest (g), the weight of tubers at harvest (g), root weight at harvest (g). The results showed that the application biokompos "Trichosubur Protect" effective (significant) to the weight of onion bulbs (Allium ascalonicum L) varieties Palu valley during harvest. P4 is the best treatment compared with other treatments, is seen in the average weight of leaves at harvest (7.21 g), the weight of tubers at harvest (18.73 g), root weight at harvest (0.87 g).
Formulasi Substrat Dasar Kotoran Sapi dan Limbah Cair Tempe dengan menggunakan Inokulum Rumen Sapi Untuk Studi Awal Produksi Biogas Bidayanti Ruru; Umrah Umrah; Orryani Lambui
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research of “Biogas Production by Utilizing of Basic Substrate of  Fermented Liquid waste of tempeh and Cow Waste by Using Inoculum from Cow Rumen” for the basic study of biogas production was held on May until July 2016 at department Biology Laboratory unit Biotechnology, Faculty of Mathematic and Natural Sciences, University of Tadulako. The purposed of this research is to produce biogas by utilizing the basic of liguid waste of tempeh and cow waste, and also to know the concentration of using the rumen inoculum of cow rumen. This research is designed with completely randomized design which consists of six treatment with three repeating. the basic substance are composed of 500 grams of cow dung and 500 ml of liquid waste tempe.The composition of treatment is : ( P0 100% Basic Substrate Without inoculum),  ( P1 80% Basic Substrate + 20% inoculums) ,(P2 60% Basic Substrate + 40% inoculums), (P3 40% Basic Substrate + 60% inoculums) , (P3 20% Basic Substrate + 80% inoculums), (P5 100% inoculum Without basic substrate). Observation variable are consist of biogas volume measuring, pH measuring, light test and temperature measuring. anaerobic fermentation process is carried out in a culture bottle with a volume of 1000 ml bottle mouth top covered with a balloon for gas storage. The results of this research show that Biogas could be produced by using the basic substrate of fermented liguid waste of tempeh and cow waste with the inoculum from cow rumen, at, P2, P3, dan P4 treatment. The highest volume of gas in P2 treatment (60% Basic Substrate : 40% inoculum) which has 553,33 cm3. The pH which in the culture is about 6,33-6,46 and the result flame with temperature in 152ºC.
UJI KEEFEKTIFAN PUPUK BIOKOMPOS “ Trichosubur Protect “ TERHADAP PERTUMBUHAN BIBIT KAKAO ( Theobroma Cacao L ) Evisilvia Evisilvia; Umrah Umrah; Yusran Yusran
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Research on the effectivenes of the test biokompos ’’ Trichosubur Protect’’on the growth of the cacao plant seeds have been conducted from November 2015 through march 2016 at the cacao gardens hortus Botanicus ( Botanical Garden ) department of Biology, Faculty of Mathematics and Natural Sciences Tadulako University, Palu- Central Sulawesi. The objective is to determine the dose biokompos “ Trichosubur Protect “ which is effective against the growth of cacao seedlings. The study was designed in a compososition of the treament as fllows : P0 ( Basal medium without “ Trichosubur Protect “),P1 ( Basic medium + ’’ Trichosubur Protect “ 10 g), P2 ( basic medium + “ Trichosubur Protect “ 20 g), P3 ( Basic medium + “ Trichosubur Protect “ 30 g), P4 ( Basic medium + “ Trichosubur Protect” 40 g), P5 ( medium Ground + NPK 2 g). Basic media used in the form of a mixture of soil 19 kg + 1 kg of rice husk, put in a polybag 25 x 30 cm 13 kg as the planting of cacao. Parameters include observavation every week; seedling height (cm), number of leaves ( leaf),the girth (mm).observation at harvest inculude: Biomassa (g),leaf weight (g),the weight rod (g), root weight(g),and root dry weight (g). The result shwed that treament P4 ( basic + “ Trichosubur Protect “ 40 g) the treatment is more efeective when seen from the results of last observatoins covering, the everage 29,07 cm plant height, leaf number 40 strands, stem diameter 3,73 cm, weight of leaves at harvest time 12,03 g ( Moisture in the leaves 44,30 g ),weight at harvest stalks of 7,62 g( moisture in the trunk 56,30 g), root weight at harvest 5,93g ( Moisture at the root of 92,80 g), the weight of biomassa when harvested 27,22 g, 4,42 g dry weight of leaf, stem dry weight of 1,98 g, 8,06 g dry weight biomassa.
STUDI TANAMAN BERKHASIAT OBAT SUKU MORI DI KECAMATAN PETASIA, PETASIA BARAT, DAN PETASIA TIMUR KABUPATEN MOROWALI UTARA SULAWESI TENGAH Idris Idris; Nurlina Ibrahim; Arsa Wahyu Nugrahani
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

This study aims to conduct an inventory, knowing how to use and organs of plants used as medicine by Mori Tribal in Petasia, Petasia and East Petasia District, West North Morowali Regency of Central Sulawesi. This study used qualitative methods and sampling techniques that snowball sampling, with open-ended interview techniques interview on media 9 informants obtained using a questionnaire. Based on the results of research known as 79 species of medicinal plants, 2 of which can not be identified and divided into 45 familia used as a medicine. Plants are most widely used is of familia Euphorbiaceae as many as 8 species. Organs of plants used include leaves, stems, fruits, rhizomes, seeds, bark, flowers, bulbs, herbaceous roots and sap. Organ plant is widely used is the leaves that percentage utilization is 58%. Mori Tribal communities in the District, West Petasia and East Petasia North Morowali District use medicinal herbs to treat the disease, healing and health maintenance. The cooking methods include boiling water, brewed, boxed, taped, squeezed, scrubbed/smeared, chewed and consumed directly, drops, and sprayed. How to use include drunk, eaten, chewed and consumed directly, polished/smeared, attached, dropped, and chewed and sprayed on the affected area.
KAJIAN ADAPTASI VARIETAS UNGGUL BARU PADI DAN TINGKAT SERANGAN ORGANISMA PENGGANGGU TANAMAN PADA DUA LOKASI SENTRA PADI DI PROVINSI GORONTALO Warda Warda
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Averaged productivity of paddy in Gorontalo on 2009 was 4.56 t / ha. The productivity of paddy plants is lower compared with the potential yields that can produce 8.0 t / ha. One of the causing problems of low productivity is the attack of pests. Paddy plants have many pests, primary and non-primary. Litbang has released many new superior varieties (NSV) of paddy rice with various advantages of each variety, especially resistance to certain pests. However, some varieties do not show resistance to pests in certain agroecosystems. The objective of the assessment was to obtain resistant or tolerant tolerant paddy field NSV in the Gorontalo agroecosystem. The assessment consisted of two experiments ie : (1) Assessment of NSV resistance to pest attack and (2) Survey of the type and level of rice pest populations in the two selected districts. The study used Randomized Block Design and survey methods in Kabupaten Gorontalo and Boalemo from January to December 2012. The paddy field NSVs in the test were Inpari 2, Inpari 3, Inpari 4, Inpari 6, Inpari 7, Inpari 9, Inpari 10 and Inpari 13 and Ciherang and memberamo as control / comparison. Inpari 4 and Inpari 10 varieties tend to have resistance to pests and have high yield potential in the field. The dominant and potentially destructive pests in each plant stadia are stem borer, crackle, HPP, grayak caterpillar and earthen bundle. The natural enemies that are dominant in helping balance natural ecosystems are spiders and telenomus.
EFEK ANTIPIRETIK EKSTRAK DAUN PALIASA (Kleinhovia hospita L.) TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus L.) YANG DIINDUKSI VAKSIN DIFTERI PERTUSIS TETANUS Shella Desiana; Yuliet Yuliet; Ihwan Ihwan
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Paliasa (Kleinhovia hospita L.) is a plant that is empirically used as a fever-lowering drugs. Antipyretic activity test Paliasa leaf ethanol extract of the white male rats (Rattus norvegicus) aims to determine the effect of the antipyretic effect of ethanol extract of leaves Paliasa and determine the effective dose as an antipyretic. Tests were carried out using inducers of fever vaccine Diphtheria Tetanus Pertussis (DPT) 0.2 ml. The antipyretic tests effect is divided into six treatment groups. The first group was given Na CMC 1% and DPT vaccine induced as a negative control, a group of the 2nd given paracetamol as a positive control, all three groups were given Na CMC 1% as the normal control group 4th, 5th, and 6th each given Paliasa leaf ethanol extract at a dose of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW. Rectal temperature measurements carried out for 6 hours with a 30 minute interval. Temperature measurement data were statistically analyzed using One way ANOVA followed by Duncan test at 95% confidence level. Antipyretic activity test results showed that the ethanol extract of leaves Paliasa have antipyretic activity in male rats and the effective dose is 200 mg/kg BW.
Uji Aktivitas Antibakteri Ekstrak Serbuk Gergaji Kayu Eboni (Diospyros celebicaBakh.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli Wahyuni Wahyuni; Nurlina Ibrahim; Arsa Wahyu Nugrahani
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Ebony (Diospyros celebica Bakh.) is one of the beneficial plants from ebenaceae, especially in traditional medicine. The sawdust extract containing chemical compounds such as tannins, saponins and terpenoids. This research were aimed to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) also to identified the active compounds from sawdust extracts of D. celebica Bakh. against Staphylococcus aureus and Escherichia coli. The Research methods include extraction using maceration method by ethanol 96%. MIC and MBC determination using dilution and diffusion methods. The Results showed that MIC value of S.aureus and E. coli were 6% and 7%, respectively. While MBC value of S. aureus and E. coli were 12% and 13%, respectively. Identification of chemical compounds were determined by Thin Layer Chromatography (TLC) bioautography method by 10% H2SO4 and anisaldehid-sulfuric acid reagent spray. There were two chemichal compounds that produced, saponin and terpenoid.

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